Vaccine composition

ABSTRACT

The present invention relates to the field of Gram-negative bacterial vaccine compositions, their manufacture, and the use of such compositions in medicine. More particularly it relates to the field of useful Gram-negative bacterial outer membrane vesicle (or bleb) compositions comprising heterologously expressed Chlamydia antigens, and advantageous methods of rendering these compositions more effective and safer as a vaccine.

CROSS REFERENCE TO RELATED APPLICATIONS

[0001] This application is a National Stage Application filed under 35 U.S.C. §371 of International Application No: PCT/EP02/01356, filed 08 Feb. 2002.

FIELD OF THE INVENTION

[0002] The present invention relates to the field of Gram-negative bacterial vaccine compositions, their manufacture, and the use of such compositions in medicine. More particularly it relates to the field of useful Gram-negative bacterial outer membrane vesicle (or bleb) compositions comprising heterologously expressed Chlamydia antigens, and advantageous methods of rendering these compositions more effective and safer as a vaccine.

BACKGROUND OF THE INVENTION

[0003] Chlamydiae are obligate intracellular Gram negative bacteria which replicate only in cytoplasmic inclusions of eukaryotic cells. They have a unique developmental cycle which is represented by two major forms, the spore-like elementary body (EB) which is the infectious form transmitted from cell to cell, and the non infectious, metabolically active reticulate body (RB) which replicates within the host-cell.

[0004] Of the four known chlamydial species, Chlamydia trachomatis and C. pneumoniae are the important human pathogens. The recently defined species C. pneumoniae (Grayston 1989) is now recognized as a major cause of respiratory tract infections (Grayston 1993) and data are now growing for an association with atherosclerosis. The association is supported by seroepidemiological studies, studies demonstrating the presence of the bacterium in the atherosclerotic lesions, studies showing C. pneumoniae capability to replicate in the different cell types present in the atheroclerotic lesions, interventional trials with antibiotics in patients with coronary artery disease and experimental respiratory tract infection in rabbits or apolipoprotein-E deficient mice which leads to inflammatory changes in the aorta (Danesh 1997, Fong 1997, Laitinen 1997, Moazed 1997). Overall, those data implicate C. pneumoniae as a causative and/or aggravating factor of atherosclerosis.

[0005]C. trachomatis is a major human pathogen; transmitted from human to human (there is no known animal reservoir), it causes ocular and genital infections which can result in long term sequelae. Trachoma, a chlamydial ocular infection, is endemic in several developing countries and is the world's leading cause of preventable blindness with millions people affected by the disease. Genital chlamydial infections constitute the most common bacterial sexually transmitted disease (STD) worldwide. In 1996, WHO generated a new set of global estimates for four major STDs drawing an extensive review of the published and unpublished prevalence data (Gerbase 1998). It has been estimated that in 1995, 4 and 5.2 million new cases of C. trachomatis infection occured in individuals aged 15-49 for North America and Western Europe, respectively; worlwide, C. trachomatis totalized an estimate of 89.1 million new cases. Collectively, data show higher infection rates in women as compared to men (Washington 1987, Peeling 1995, Cates 1991); higher incidence is found in adolescent and young adults, approximately 70% of the chlamydial infections being reported in the 15-24 years of age group (Peeling 1995).

[0006] There is a clear need for effective vaccines against Chlamydia trachomatis and Chlamydia pneumoniae.

[0007] Outer Membrane Vesicles (blebs)

[0008] Gram-negative bacteria are separated from the external medium by two successive layers of membrane structures. These structures, referred to as the cytoplasmic membrane and the outer membrane (OM), differ both structurally and functionally. The outer membrane plays an important role in the interaction of pathogenic bacteria with their respective hosts. Consequently, the surface exposed bacterial molecules represent important targets for the host immune response, making outer-membrane components attractive candidates in providing vaccine, diagnostic and therapeutics reagents.

[0009] Whole cell bacterial vaccines (killed or attenuated) have the advantage of supplying multiple antigens in their natural micro-environment. Drawbacks around this approach are the side effects induced by bacterial components such as endotoxin and peptidoglycan fragments. On the other hand, acellular subunit vaccines containing purified components from the outer membrane may supply only limited protection and may not present the antigens properly to the immune system of the host.

[0010] Proteins, phospholipids and lipopolysaccharides are the three major constituents found in the outer-membrane of all Gram-negative bacteria. These molecules are distributed asymmetrically: membrane phospholipids (mostly in the inner leaflet), lipooligosaccharides (exclusively in the outer leaflet) and proteins (inner and outer leaflet lipoproteins, integral or polytopic membrane proteins). For many bacterial pathogens which impact on human health, lipopolysaccharide and outer-membrane proteins have been shown to be immunogenic and amenable to confer protection against the corresponding disease by way of immunization.

[0011] The OM of Gram-negative bacteria is dynamic and, depending on the environmental conditions, can undergo drastic morphological transformations. Among these manifestations, the formation of outer-membrane vesicles or “blebs” has been studied and documented in many Gram-negative bacteria (Zhou, L et al. 1998. FEMS Microbiol. Lett. 163: 223-228). Among these, a non-exhaustive list of bacterial pathogens reported to produce blebs include: Bordetella pertussis, Borrelia burgdorferi, Brucella melitensis, Brucella ovis, Chlamydia psittaci, Chlamydia trachomatis, Esherichia coli, Haemophilus influenzae, Legionella pneumophila, Neisseria gonorrhoeae, Neisseria meningitidis, Pseudomonas aeruginosa and Yersinia enterocolitica. Although the biochemical mechanism responsible for the production of OM blebs is not fully understood, these outer membrane vesicles have been extensively studied as they represent a powerful methodology in order to isolate outer-membrane protein preparations in their native conformation.

[0012] Examples of bacterial species from which bleb vaccines can be made have been reviewed in WO 01/09350 (incorporated by reference herein). For example, N. meningitidis serogroup B (menB) excretes outer membrane blebs in sufficient quantities to allow their manufacture on an industrial scale. Such multicomponent outer-membrane protein vaccines from naturally-occurring menB strains have been found to be efficacious in protecting teenagers from menB disease and have become registered in Latin America. An alternative method of preparing outer-membrane vesicles is via the process of detergent extraction of the bacterial cells (EP 11243).

SUMMARY OF THE INVENTION

[0013] The present inventors have found that Gram-negative bacterial blebs are an ideal context to present Chlamydia outer membrane proteins. In particular gonococcal blebs are useful in the case of presenting C. trachomatis OMPs and meningococcal blebs are useful in the case of presenting C. pneumoniae OMPs. This is because a) these outer-membrane proteins can integrate into such blebs in a native (or near-native) conformation thus retaining a useful immunological effect; b) blebs (particularly from Neisseria strains) can be produced in industrial quantities, c) blebs may be mucosally administered, and d) the combination of Chlamydia antigens with native bleb antigens can have important interactions for certain conditions such as salpingitis.

[0014] The present invention thus provides advantageous Gram-negative bacterial bleb preparations (derived from bleb-producing bacterial strains listed above, and preferably not derived from Chlamydia) presenting on its surface one or more recombinant (and preferably heterologous) protein antigens from Chlamydia trachomatis or Chlamydia pneumoniae. Advantagous vaccine formulations and methods of administration are also provided.

DESCRIPTION OF THE INVENTION

[0015] The present invention provides a Gram-negative bacterial bleb presenting on its surface one or more outer membrane protein from Chlamydia.

[0016] In the context of this application the term “presenting on its surface” indicates that the Chlamydia protein should be exposed to the outer surface of the bleb and tethered to the outer membrane (preferably by being integrated into the outer membrane). Most preferably it should take up its native fold within the heterologous bleb context.

[0017] An efficient strategy to modulate the composition of a Bleb preparation in this way is to deliver one or more copies of a DNA segment containing an expression cassette comprising a gene encoding said Chlamydia outer membrane protein into the genome of a Gram-negative bacterium.

[0018] A non exhaustive list of preferred bacterial species that could be used as a recipient for such a cassette includes: Bordetella pertussis, Borrelia burgdorferi, Brucella melitensis, Brucella ovis, Chlamydia psittaci, Chlamydia trachomatis, Esherichia coli, Haemophilus influenzae, Legionella pneumophila, Neisseria gonorrhoeae, Neisseria meningitidis, Pseudomonas aeruginosa and Yersinia enterocolitica. Neisseria meningitidis, Neisseiria gonorrhoeae, Moraxella catarrhalis, Haemophilus influenzae, Pseudomonas aeruginosa, Chlamydia trachomatis, Chlamydia pneumoniae are more preferred for this purpose, and Neisseria gonorrhoeae and Neisseria meningitidis are most preferred for making the blebs of this invention. Preferably the Chlamydia OMPs are expressed heterologously, and in such situations Chlamydia strains should not be used to make the blebs of the invention.

[0019] The gene(s) contained in the expression cassette may be homologous (or endogenous) (i.e. exist naturally in the genome of the manipulated bacterium) or, preferably, heterologous (i.e. do not exist naturally in the genome of the manipulated bacterium). The introduced expression cassette may consist of unmodified, “natural” promoter/gene/operon sequences or engineered expression cassettes in which the promoter region and/or the coding region or both have been altered. A non-exhaustive list of preferred promoters (preferably strong) that could be used for expression includes the promoters porA, porB, lbpB, tbpB, p110, lst, hpuAB from N. meningitidis or N. gonorroheae, the promoters p2, p5, p4, ompF, p1, ompH, p6, hin47 from H. influenzae, the promoters ompH, ompG, ompCD, ompE, ompB1, ompB2, ompA of M. catarrhalis, the promoter •pL, lac, tac, araB of Escherichia coli or promoters recognized specifically by bacteriophage RNA polymerase such as the E. coli bacteriophage T7.

[0020] In a preferred embodiment of the invention the expression cassette is delivered and integrated in the bacterial chromosome by means of homologous and/or site specific recombination (as discussed in WO 01/09350 incorporated by reference herein). Integrative vectors used to deliver such genes and/or operons can be conditionally replicative or suicide plasmids, bacteriophages, transposons or linear DNA fragments obtained by restriction hydrolysis or PCR amplification. Integration is preferably targeted to chromosomal regions dispensable for growth in vitro. A non exhaustive list of preferred loci that can be used to target DNA integration includes the porA, porB, opa, opc, rmp, omp26, lecA, cps, lgtB genes of Neisseiria meningitidis and Neisseria gonorrhoeae, the P1, P5, hmw1/2, IgA-protease, fimE genes of NTHi; the lecA1, lecA2, omp106, uspA1, uspA2 genes of Moraxella catarrhalis. Alternatively, the expression cassette used to modulate the expression of bleb component(s) can be delivered into a bacterium of choice by means of episomal vectors such as circular/linear replicative plasmids, cosmids, phasmids, lysogenic bacteriophages or bacterial artificial chromosomes. Selection of the recombination event can be selected by means of selectable genetic marker such as genes conferring resistance to antibiotics (for instance kanamycin, erythromycin, chloramphenicol, or gentamycin), genes conferring resistance to heavy metals and/or toxic compounds or genes complementing auxotrophic mutations (for instance pur, leu, met, aro). Blebs may be made from the resulting modified strain.

[0021] The expression of some heterologous proteins in bacterial blebs may require the addition of outer-membrane targeting signal(s). The preferred method to solve this problem is by creating a genetic fusion between a heterologous gene and a gene coding for a resident OMP as a specific approach to target recombinant proteins to blebs. Most preferably, the heterologous gene is fused to the signal peptides sequences of such an OMP.

[0022] A particularly preferred application of this invention is the introduction of Chlamydia (trachomatis or pneumoniae) protective antigens (preferably outer membrane proteins) into Gram-negative bacterial blebs (preferably not from Chlamydia strains). This has several advantages including the fact that such blebs (and vaccines comprising them) are extremely suitable for mucosal administration, which is beneficial as a mucosal (IgA) immune response against the Chlamydia antigens present in the bleb will be more protective against Chlamydia infections which manifest themselves in the mucosa. Recombinant bacteria capable of producing blebs of the invention, processes of making such bacteria, and processes of making bleb preparations are further aspects of this invention.

[0023]Chlamydia trachomatis Antigens Integrated into a Gram Negative Bacterial bleb

[0024] A particularly preferred embodiment is in the field of the prophylaxis or treatment of sexually-transmitted diseaseses (STDs). It is often difficult for practitioners to determine whether the principal cause of a STD is due to gonococcus or Chlamydia trachomatis infection. These two organisms are major causes of salpingitis—a disease which can lead to sterility in the host. It would be useful if a STD could be vaccinated against or treated with a combined vaccine effective against disease caused by both organisms. The Major Outer Membrane Protein (MOMP or OMP1 or OMPI) of C. trachomatis has been shown to be the target of protective antibodies. However, the structural integrity of this integral membrane protein is important for inducing such antibodies. In addition, the epitopes recognised by these antibodies are variable and define more than 10 serovars. The bleb context of the invention allows the proper folding of one or more MOMP or other Chlamydia membrane proteins for vaccine purposes. The engineering of (preferably) a gonococcal strain expressing one or more C. trachomatis MOMP serovars and/or one or more other protective Chlamydia OMPs in the outer membrane, and the production of blebs therefrom, produces a single solution to the multiple problems of correctly folded membrane proteins, the presentation of sufficient MOMP serovars and/or other Chlamydia OMPs to protect against a wide spectrum of serovars, and the simultaneous prophylaxis/treatment of gonococcal infection (and consequently the non-requirement of practitioners to initially decide which organism is causing particular clinical symptoms—both organisms can be vaccinated against simultaneously thus allowing the treatment of the STD at a very early stage). Preferred loci for gene insertion in the gonoccocal chromosome are give above. Other preferred, protective C. trachomatis genes that could be incorporated are HMWP, PmpG and those OMPs disclosed in WO 99/28475 (incorporated by reference herein).

[0025] A particularly preferrred embodiment of the invention provides a Gram-negative bacterial bleb (preferably gonococcal) presenting on its surface the PorB outer membrane protein (see below) from Chlamydia trachomatis. A bacterial strain capable of producing such a bleb is a further aspect of the invention. PorB Chlamydia trachomatis serovar D (D/UW-3/Cx) DNA sequence [SEQ ID NO:1] ATGAGTAGCAAGCTAGTGAACTATCTCCGTTTGACTTTCCTATCTTTTTTAGGGATCGCATCTACTTCATT AGACGCTATGCCTGCGGGGAATCCGGCGTTTCCAGTCATCCCGGGGATTAATATTGAACAGAAAAATGCCT GTTCTTTCATTTATGTAATTCTTATGATGTACTATCCGCACTGTCCGGTAACCTGAAGCTCTGCTTCTGCG GAGATTATATCTTTTCGAAGAAGCTCAGGTAAAAGATGTCCCTGTCGTTACCTCTGTGACAACAGCTGGGG TTGGTCCTTCTCCTGATATTACTTCGACAACCAAAACGCGAAATTTCGATCTCGTGAACTGTAATCTCAAT ACAAACTGTGTAGCTGTAGCTTTTTCCCTTCCTATCGTTCGCTGAGCGCGATTCCTCTGTTTGATGTGAGT TTCGAAGTGAAAGTAGGAGGACTGAAACAATACTACCGCCTTCCCATGAATGCCTATCGAGACTTCACCTC GGAACCTCTCAATTCTGAATCAGAAGTTACGGACGGGATGATTGAAGTACGTCCAATTACGGATTTGTTTG GGATGTTAGCTTGAAAAAAGTCATATGGAAAGATGGCGTTTCCTTTGTAGGCGTCGGTCAGACTATCGCCA TGCTTCTTGCCCTATTGACTACATCATTGCAAACAGTCAAGCTAATCCAGAAGTATTCATCGCTGATCGGA TGGGAAACTGAACTTCAAGGAGTGGAGTGTCTGCGTAGGTCTTACTACCTATGTGAATGACTACGTTCTTC CTTCTTAGCGTTTTCTATAGGGAGTGTTTCTCGCCAAGCTCCGGACGACAGCTTCAAAAAATTAGAAGATC GCTTCACTAACTCAAATTTAAAGTTCGTAAAATTACCAGCTCTCATCGTGGAAACATCTGCATCGGAGCGA CAAACTATGTCGCCGATAATTCTTCTACAACGTAGAAGGAAGATGGGGAAGCCAGCGCGCTGTGAACGTCT CCGGAGGATTCCAATTCTAA Translated amino acid sequence [SEQ ID NO:2]            MSSKLVNYLR LTFLSFLGIA STSLDAMPAG NPAFPVIPGI NIEQKNACSF DLCNSYDVLS ALSGNLKLCF CGDYIFSEEA QVKDVPVVTS VTTAGVGPSP DITSTTKTRN FDLVNCNLNT NCVAVAFSLP DRSLSAIPLF DVSFEVKVGG LKQYYRLPMN AYRDFTSEPL NSESEVTDGM IEVQSNYGFV WDVSLKKVIW KDGVSFVGVG ADYRHASCPI DYIIANSQAN PEVFIADSDG KLNFKEWSVC VGLTTYVNDY VLPYLAFSIG SVSRQAPDDS FKKLEDRFTN LKFKVRKITS SHRGNICIGA TNYVADNFFY NVEGRWGSQR AVNVSGGFQF

[0026] The presence of PorB in the blebs means that the antigen can be mucosally administered more easily, and provides more effective protection than if administered alone.

[0027] The present invention additionally provides a Gram-negative bacterial bleb (preferably gonococcal) presenting on its surface one or more of the following proteins from Chlamydia trachomatis, or C. trachomatis PorB in combination with one or more of the following proteins. It will be clear to a skilled person that instead of the sequences below (and the PorB sequence above), the natural analogue of the sequences from other C. trachomatis serovars or serotypes could be used, as could genes encoding functional analogues of the proteins comprising insertions, deletions or substitutions from the recited sequences which unaffect the immunological properties of the encoded protein. Preferably a sequence from a serovar D strain should be selected. A bacterial strain capable of producing such a bleb is a further aspect of the invention. >gi|6578118|gb|AAC68456.2| predicted Protease containing IRBP and DHR domains [Chlamydia trachomatis][SEQ ID NO:3] MKMNRIWLLLLTFSSAIHSPVQGESLVCKNALQDLSFLEHLLQVKYAPKTWKEQYLGWDLVQSSVSAQQK LRTQENPSTSFCQQVLADFIGGLNDFHAGVTFFAIESAYLPYTVQKSSDGRFYFVDIMTFSSEIRVGDEL LEVDGAPVQDVLATLYGSNHKGTAAEESAALRTLFSRMASLGHKVPSGRTTLKIRRPFGTTREVRVKWRY VPEGVGDLATIAPSIRAPQLQKSMRSFFPKKDDAFHRSSSLFYSPMVPHFWAELRNHYATSGLKSGYNIG STDGFLPVIGPVIWESEGLFRAYISSVTDGDGKSHKVGFLRIPTYSWQDMEDFDPSGPPPWEEFAKIIQV FSSNTEALIIDQTNNPGGSVLYLYALLSMLTDRPLELPKHRMILTQDEVVDALDWLTLLENVDTNVESRL ALGDNMEGYTVDLQVAEYLKSFGRQVLNCWSKGDIELSTPIPLFGFEKIHPHPRVQYSKPICVLINEQDF SCADFFPVVLKDNDRALIVGTRTAGAGGFVFNVQFPNRTGIKTCSLTGSLAVREHGAFIENIGVEPHIDL PFTANDIRYKGYSEYLDKVKKLVCQLINNDGTIILAEDGSF >gi|3329331|gb|AE001359.1:101-1906[SEQ ID NO:4] ATGAAAATGAATAGGATTTGGCTATTACTGCTTACCTTTTCTTCTGCCATACATTCTCCTGTACAAGGAG AAAGCTTGGTTTGCAAGAATGCTCTTCAAGATTTGAGTTTTTTAGAGCATTTATTACAGGTTAAATATGC TCCTAAAACATGGAAAGAGCAATACTTAGGATGGGATCTTGTTCAAAGCTCCGTTTCTGCACAGCAGAAG CTTCGTACACAAGAAAATCCATCAACAAGTTTTTGCCAGCAGGTCCTTGCTGATTTTATCGGAGGATTAA ATGACTTTCACGCTGGAGTAACTTTCTTTGCGATAGAAAGTGCTTACCTTCCTTATACCGTACAAAAAAG TAGTGACGGCCGTTTCTACTTTGTAGATATCATGACTTTTTCTTCAGAGATCCGTGTTGGAGATGAGTTG CTAGAGGTGGATGGGGCGCCTGTCCAAGATGTACTCGCTACTCTATATGGAAGCAATCACAAAGGGACTG CAGCTGAAGAGTCGGCTGCTTTAAGAACACTATTTTCTCGCATGGCCTCTTTAGGGCACAAAGTACCTTC TGGGCGCACTACTTTAAAGATTCGTCGTCCTTTTGGTACTACGAGAGAAGTTCGTGTGAAATGGCGTTAT GTTCCTGAAGGTGTAGGAGATTTGGCTACCATAGCTCCTTCTATCAGGGCTCCACAGTTACAGAAATCGA TGAGAAGCTTTTTCCCTAAGAAAGATGATGCGTTTCATCGGTCTAGTTCGCTATTCTACTCTCCAATGGT TCCGCATTTTTGGGCAGAGCTTCGCAATCATTATGCAACGAGTGGTTTGAAAAGCGGGTACAATATTGGG AGTACCGATGGGTTTCTCCCTGTCATTGGGCCTGTTATATGGGAGTCGGAGGGTCTTTTCCGCGCTTATA TTTCTTCGGTGACTGATGGGGATGGTAAGAGCCATAAAGTAGGATTTCTAAGAATTCCTACATATAGTTG GCAGGACATGGAAGATTTTGATCCTTCAGGACCGCCTCCTTGGGAAGAATTTGCTAAGATTATTCAAGTA TTTTCTTCTAATACAGAAGCTTTGATTATCGACCAAACGAACAACCCAGGTGGTAGTGTCCTTTATCTTT ATGCACTGCTTTCCATGTTGACAGACCGTCCTTTAGAACTTCCTAAACATAGAATGATTCTGACTCAGGA TGAAGTGGTTGATGCTTTAGATTGGTTAACCCTGTTGGAAAACGTAGACACAAACGTGGAGTCTCGCCTT GCTCTGGGAGACAACATGGAAGGATATACTGTGGATCTACAGGTTGCCGAGTATTTAAAAAGCTTTGGAC GTCAAGTATTGAATTGTTGGAGTAAAGGGGATATCGAGTTATCAACGCCTATTCCTCTTTTTGGTTTTGA GAAGATTCATCCACATCCTCGAGTTCAATACTCTAAACCGATTTGTGTTTTGATCAATGAGCAAGACTTT TCTTGTGCTGACTTCTTCCCTGTAGTTTTGAAAGACAATGATCGAGCTCTTATTGTTGGTACTCGAACAG CTGGAGCTGGAGGATTTGTCTTTAATGTGCAGTTCCCAAATAGAACTGGAATAAAAACTTGTTCTTTAAC AGGATCATTAGCTGTTAGAGAGCATGGTGCCTTCATTGAGAACATCGGAGTCGAACCGCATATCGATCTG CCTTTTACAGCGAATGATATTCGCTATAAAGGCTATTCCGAGTATCTTGATAAGGTCAAAAAATTGGTTT GTCAGCTGATCAATAACGACGGTACCATTATTCTTGCGGAAGATGGTAGTTTTTAA >gi|6578109|gb|AAC68227.2| CHLPN 76kDa Homolog [Chlamydia trachomatis][SEQ ID NO:5] MKKYFYKGFVGALLLACGSTNLAFAQASSMDSQLWSVEDLDSYLSSKGFVETRKRDGVLRLAGDVRARWI YAKEDLETTQTPAKPMLPTNRYRSEFNLYVDYTAANSWMTSKMNWVTIAGGESSAAGLDINRAFLGYRFY KNPETQAEVFAEIGRSGLGDIFDSDVQFNSNFDGIHLYAARRISEKLPFTMIVHGGPFVVNMAEKEYAWV VEAILNKLPGNFVVKTSVVDWNTLTAKTNDPADASAAQPAKPNTKYDYLVWQWLVGKSTAMPWFNGQTKN LYTYGAYLFNPLAEIPENWKQSTTPTTKITNGKENHAWFIGCSLGGVRRAGDWSATVRYEYVEALAIPEI DVAGIGRGNQMKYWFAQAIKQGLDPKESNGFTNYKGVSYQFVMGLTDSVSFRAYAAYSKPANDNLGSDFT YRKYDLGLISSF >gi|3329068|gb|AE001333.1:c3495-2197[SEQ ID NO:6] ATGAAAAAATACTTTTATAAAGGGTTTGTAGGCGCGCTTTTATTAGCTTGTGGGTCTACAAACTTGGCTT TTGCGCAGGCTAGTTCGATGGATAGCCAGCTATGGTCTGTTGAAGATTTAGATTCTTATTTGAGTTCCAA AGGTTTTGTCGAGACTCGTAAGCGCGATGGAGTTCTACGTTTAGCTGGAGATGTCCGCGCTCGATGGATT TATGCAAAAGAGGATCTTGAGACAACTCAGACTCCTGCTAAACCTATGTTACCTACCAATCGGTATCGTA GTGAATTCAATTTGTATGTGGATTACACCGCTGCTAATAGTTGGATGACTTCGAAAATGAATTGGGTAAC GATTGCTGGCGGAGAATCTTCTGCAGCAGGGTTAGATATTAATCGTGCCTTCTTAGGATACCGATTCTAC AAAAACCCAGAAACGCAAGCAGAAGTATTTGCAGAGATTGGTCGCTCTGGATTGGGAGATATTTTTGATT CCGACGTTCAGTTTAATAGTAATTTCGACGGAATTCATTTATACGCTGCGCGACGTATTAGTGAGAAACT TCCTTTCACCATGATTGTTCATGGTGGTCCTTTTGTCGTGAATATGGCAGAGAAAGAGTATGCTTGGGTC GTGGAAGCTATTTTGAATAAACTCCCAGGAAATTTCGTTGTGAAAACGAGTGTTGTTGACTGGAATACGT TAACAGCAAAAACGAATGATCCAGCAGACGCAAGCGCTGCACAACCAGCTAAACCTAATACCAAGTACGA TTATTTAGTATGGCAATGGTTGGTTGGGAAGAGCACAGCTATGCCATGGTTTAATGGACAAACAAAAAAT CTTTACACTTACGGAGCCTATCTCTTTAATCCATTAGCGGAAATACCAGAGAACTGGAAACAATCAACAA CTCCTACAACCAAAATTACAAATGGTAAGGAAAACCATGCTTGGTTCATCGGCTGCTCTCTAGGCGGTGT TCGACGAGCTGGAGACTGGTCTGCAACAGTTCGTTATGAGTATGTTGAAGCTTTAGCGATTCCAGAAATT GATGTCGCGGGTATTGGTCGCGGAAACCAAATGAAATATTGGTTTGCTCAAGCTATCAAACAAGGATTGG ATCCTAAAGAATCTAACGGCTTTACTAACTATAAAGGAGTTTCCTATCAGTTTGTTATGGGTCTGACAGA TTCGGTTTCTTTCCGAGCTTATGCTGCTTATTCTAAGCCTGCTAACGATAACCTTGGTAGCGACTTCACC TATCGTAAGTATGACCTAGGTTTAATTTCTTCATTCTAA >gi|3329350|gb|AAC68472.1| Putative Outer Membrane Protein I [Chlamydia trachomatis][SEQ ID NO:7] MRPDHMNFCCLCAAILSSTAVLFGQDPLGETALLTKNPNHVVCTFFEDCTMESLFPALCAHASQDDPLYV LGNSYCWFVSKLHITDPKEALFKEKGDLSIQNFRFLSFTDCSSKESSPSIIHQKNGQLSLRNNGSMSFCR NHAEGSGGAISADAFSLQHNYLFTAFEENSSKGNGGAIQAQTFSLSRNVSPISFARNRADLNGGAICCSN LICSGNVNPLFFTGNSATNGGAICCISDLNTSEKGSLSLACNQETLFASNSAKEKGGAIYAKHMVLRYNG PVSFINNSAKIGGAIAIQSGGSLSILAGEGSVLFQNNSQRTSDQGLVRNAIYLEKDAILSSLEARNGDIL FFDPIVQESSSKESPLPSSLQASVTSPTPATASPLVIQTSANRSVIFSSERLSEEEKTPDNLTSQLQQPI ELKSGRLVLKDRAVLSAPSLSQDPQALLIMEAGTSLKTSSDLKLATLSIPLHSLDTEKSVTIHAPNLSIQ KIFLSNSGDENFYENVELLSKEQNNIPLLTLSKEQSHLHLPDGNLSSHFGYQGDWTFSWKDSDEGHSLIA NWTPKNYVPHPERQSTLVANTLWNTYSDMQAVQSMINTIAHGGAYLFGTWGSAVSNLFYAHDSSGKPIDN WHHRSLGYLFGISTHSLDDHSFCLAAGQLLGKSSDSFITSTETTSYIATVQAQLATPLMKISAQACYNES IHELKTKYRSFSKEGFGSWHSVAVSGEVCASIPIVSNGSGLFSSFSIFSKLQGFSGTQDGFEESSGEIRS FSASSFRNISLPMGITFEKKSQKTRNYYYFLGAYIQDLKRDVESGPVVLLKNAVSWDAPMANLDSRAYMF RLTNQRALHRLQTLLNVSYVLRGQSHSYSLDLGTTYRF >gi|3329348|gb|AE001361.1:c3451-815[SEQ ID NO:8] ATGCGACCTGATCATATGAACTTCTGTTGTCTATGTGCTGCTATTTTGTCATCCACAGCGGTCCTCTTTG GCCAGGATCCCTTAGGTGAAACCGCCCTCCTCACTAAAAATCCTAATCATGTCGTCTGTACATTTTTTGA GGACTGTACCATGGAGAGCCTCTTTCCTGCTCTTTGTGCTCATGCATCACAAGATGATCCTTTGTATGTA CTTGGAAATTCCTACTGTTGGTTCGTATCTAAACTCCATATCACGGACCCCAAAGAGGCTCTTTTTAAAG AAAAAGGAGATCTTTCCATTCAAAATTTTCGCTTCCTTTCCTTCACAGATTGCTCTTCCAAGGAAAGCTC TCCTTCTATTATTCATCAAAAGAATGGTCAGTTATCCTTGCGCAATAATGGTAGCATGAGTTTCTGTCGA AATCATGCTGAAGGCTCTGGAGGAGCCATCTCTGCGGATGCCTTTTCTCTACAACACAACTATCTTTTCA CAGCTTTTGAAGAGAATTCTTCTAAAGGAAATGGCGGAGCCATTCAGGCTCAAACCTTCTCTTTATCTAG AAATGTGTCGCCTATTTCTTTCGCCCGTAATCGTGCGGATTTAAATGGCGGCGCTATTTGCTGTAGTAAT CTTATTTGTTCAGGGAATGTAAACCCTCTCTTTTTCACTGGAAACTCCGCCACGAATGGAGGCGCTATTT GTTGTATCAGCGATCTAAACACCTCAGAAAAAGGCTCTCTCTCTCTTGCTTGTAACCAAGAAACGCTATT TGCAAGCAATTCTGCTAAAGAAAAAGGCGGGGCTATTTATGCCAAGCACATGGTATTGCGTTATAACGGT CCTGTTTCCTTCATTAACAACAGCGCTAAAATAGGTGGAGCTATCGCCATCCAGTCCGGAGGGAGTCTCT CTATCCTTGCAGGTGAAGGATCTGTTCTGTTCCAGAATAACTCCCAACGCACCTCCGACCAAGGTCTAGT AAGAAACGCCATCTACTTAGAGAAAGATGCGATTCTTTCTTCCTTAGAAGCTCGCAACGGAGATATTCTT TTCTTTGATCCTATTGTACAAGAAAGTAGCAGCAAAGAATCGCCTCTTCCCTCCTCTTTGCAAGCCAGCG TGACTTCTCCCACCCCAGCCACCGCATCTCCTTTAGTTATTCAGACAAGTGCAAACCGTTCAGTGATTTT CTCGAGCGAACGTCTTTCTGAAGAAGAAAAAACTCCTGATAACCTCACTTCCCAACTACAGCAGCCTATC GAACTGAAATCCGGACGCTTAGTTTTAAAAGATCGCGCTGTCCTTTCCGCGCCTTCTCTCTCTCAGGATC CTCAAGCTCTCCTCATTATGGAAGCGGGAACTTCTTTAAAAACTTCCTCTGATTTGAAGTTAGCTACGCT AAGTATTCCCCTTCATTCCTTAGATACTGAAAAAAGCGTAACTATCCACGCCCCTAACCTTTCTATCCAA AAGATCTTCCTCTCTAATTCTGGAGATGAGAATTTTTATGAAAATGTAGAGCTTCTCAGTAAAGAGCAAA ACAATATTCCTCTCCTTACTCTCTCTAAAGAGCAATCTCATTTACATCTTCCTGATGGGAACCTCTCTTC TCACTTTGGATATCAAGGAGATTGGACTTTTTCTTGGAAAGATTCTGATGAAGGGCATTCTCTGATTGCT AATTGGACGCCTAAAAACTATGTGCCTCATCCAGAACGTCAATCTACACTCGTTGCGAACACTCTTTGGA ACACCTATTCCGATATGCAAGCTGTGCAGTCGATGATTAATACAATAGCGCACGGAGGAGCCTATCTATT TGGAACGTGGGGATCTGCTGTTTCTAATTTATTCTATGCTCACGACAGCTCTGGGAAACCTATCGATAAT TGGCATCATAGAAGCCTTGGCTACCTATTCGGTATCAGTACTCACAGTTTAGATGACCATTCTTTCTGCT TGGCTGCAGGACAATTACTCGGGAAATCGTCCGATTCCTTTATTACGTCTACAGAAACGACCTCCTATAT AGCTACTGTACAAGCGCAACTCGCTACCCCTCTAATGAAAATCTCTGCACAGGCATGCTATAATGAAAGT ATCCATGAGCTAAAAACAAAATATCGCTCCTTCTCTAAAGAAGGATTCGGATCCTGGCATAGCGTTGCAG TATCCGGAGAAGTGTGCGCATCGATTCCTATTGTATCCAATGGTTCCGGACTGTTCAGCTCCTTCTCTAT TTTCTCTAAACTGCAAGGATTTTCAGGAACACAGGACGGTTTTGAGGAGAGTTCGGGAGAGATTCGGTCC TTTTCTGCCAGCTCTTTCAGAAATATTTCACTTCCTATGGGAATAACATTTGAAAAAAAATCCCAAAAAA CACGAAACTACTATTACTTTCTGGGAGCCTACATCCAAGACCTAAAACGTGATGTGGAATCGGGACCTGT AGTGTTACTCAAAAATGCCGTCTCCTGGGATGCTCCTATGGCGAACTTGGATTCGCGAGCCTACATGTTC AGGCTTACGAATCAAAGAGCTCTGCATAGACTTCAGACGCTGTTAAATGTGTCTTACGTACTGCGCGGGC AAAGCCATAGTTACTCCCTGGATCTGGGGACCACTTACAGGTTCTAG >gi|3329346|gb|AAC68469.1| Putative Outer Membrane Protein G [Chlamydia trachomatis][SEQ ID NO:9] MQTSFHKFFLSMILAYSCCSLSGGGYAAEIMIPQGIYDGETLTVSFPYTVIGDPSGTTVFSAGELTLKNL DNSIAALPLSCFGNLLGSFTVLGRGHSLTFENIRTSTNGAALSDSANSGLFTIEGFKELSFSNCNSLLAV LPAATTNNGSQTPTTTSTPSNGTIYSKTDLLLLNNEKFSFYSNLVSGDGGAIDAKSLTVQGISKLCVFQE NTAQADGGACQVVTSFSAMANEAPIAFIANVAGVRGGGIAAVQDGQQGVSSSTSTEDPVVSFSRNTAVEF DGNVARVGGGIYSYGNVAFLNNGKTLFLNNVASPVYIAAEQPTNGQASNTSDNYGDGGAIFCKNGAQAAG SNNSGSVSFDGEGVVFFSSNVAAGKGGAIYAKKLSVANCGPVQFLGNIANDGGAIYLGESGELSLSADYG DIIFDGNLKRTAKENAADVNGVTVSSQAISMGSGGKITTLRAKAGHQILFNDPIEMANGNNQPAQSSEPL KINDGEGYTGDIVFANGNSTLYQNVTIEQGRIVLREKAKLSVNSLSQTGGSLYMEAGSTLDFVTPQPPQQ PPAANQLITLSNLHLSLSSLLANNAVTNPPTNPPAQDSHPAIIGSTTAGSVTISGPIFFEDLDDTAYDRY DWLGSNQKIDVLKLQLGTQPSANAPSDLTLGNEMPKYGYQGSWKLAWDPNTANNGPYTLKATWTKTGYNP GPERVASLVPNSLWGSILDIRSAHSAIQASVDGRSYCRGLWVSGVSNFFYHDRDALGQGYRYISGGYSLG ANSYFGSSMFGLAFTEVFGRSKDYVVCRSNHHACIGSVYLSTKQALCGSYLFGDAFIRASYGFGNQHMKT SYTFAEESDVRWDNNCLVGEIGVGLPIVITPSKLYLNELRPFVQAEFSYADHESFTEEGDQARAFRSGHL MNLSVPVGVKFDRCSSTHPNKYSFMGAYICDAYRTISGTQTTLLSHQETWTTDAFHLARHGVIVRGSMYA SLTSNIEVYGHGRYEYRDTSRGYGLSAGSKVRF >gi|3329342|gb|AE001360.1:7736-10777[SEQ ID NO:10] ATGCAAACGTCTTTCCATAAGTTCTTTCTTTCAATGATTCTAGCTTATTCTTGCTGCTCTTTAAGTGGGG GGGGGTATGCAGCAGAAATCATGATTCCTCAAGGAATTTACGATGGGGAGACGTTAACTGTATCATTTCC CTATACTGTTATAGGAGATCCGAGTGGGACTACTGTTTTTTCTGCAGGAGAGTTAACGTTAAAAAATCTT GACAATTCTATTGCAGCTTTGCCTTTAAGTTGTTTTGGGAACTTATTAGGGAGTTTTACTGTTTTAGGGA GAGGACACTCGTTGACTTTCGAGAACATACGGACTTCTACAAATGGAGCTGCACTAAGTGACAGCGCTAA TAGCGGGTTATTTACTATTGAGGGTTTTAAAGAATTATCTTTTTCCAATTGCAACTCATTACTTGCCGTA CTGCCTGCTGCAACGACTAATAATGGTAGCCAGACTCCGACGACAACATCTACACCGTCTAATGGTACTA TTTATTCTAAAACAGATCTTTTGTTACTCAATAATGAGAAGTTCTCATTCTATAGTAATTTAGTCTCTGG AGATGGGGGAGCTATAGATGCTAAGAGCTTAACGGTTCAAGGAATTAGCAAGCTTTGTGTCTTCCAAGAA AATACTGCTCAAGCTGATGGGGGAGCTTGTCAAGTAGTCACCAGTTTCTCTGCTATGGCTAACGAGGCTC CTATTGCCTTTATAGCGAATGTTGCAGGAGTAAGAGGGGGAGGGATTGCTGCTGTTCAGGATGGGCAGCA GGGAGTGTCATCATCTACTTCAACAGAAGATCCAGTAGTAAGTTTTTCCAGAAATACTGCGGTAGAGTTT GATGGGAACGTAGCCCGAGTAGGAGGAGGGATTTACTCCTACGGGAACGTTGCTTTCCTGAATAATGGAA AAACCTTGTTTCTCAACAATGTTGCTTCTCCTGTTTACATTGCTGCTGAGCAACCAACAAATGGACAGGC TTCTAATACGAGTGATAATTACGGAGATGGAGGAGCTATCTTCTGTAAGAATGGTGCGCAAGCAGCAGGA TCCAATAACTCTGGATCAGTTTCCTTTGATGGAGAGGGAGTAGTTTTCTTTAGTAGCAATGTAGCTGCTG GGAAAGGGGGAGCTATTTATGCCAAAAAGCTCTCGGTTGCTAACTGTGGCCCTGTACAATTCTTAGGGAA TATCGCTAATGATGGTGGAGCGATTTATTTAGGAGAATCTGGAGAGCTCAGTTTATCTGCTGATTATGGA GATATTATTTTCGATGGGAATCTTAAAAGAACAGCCAAAGAGAATGCTGCCGATGTTAATGGCGTAACTG TGTCCTCACAAGCCATTTCGATGGGATCGGGAGGGAAAATAACGACATTAAGAGCTAAAGCAGGGCATCA GATTCTCTTTAATGATCCCATCGAGATGGCAAACGGAAATAACCAGCCAGCGCAGTCTTCCGAACCTCTA AAAATTAACGATGGTGAAGGATACACAGGGGATATTGTTTTTGCTAATGGAAACAGTACTTTGTACCAAA ATGTTACGATAGAGCAAGGAAGGATTGTTCTTCGTGAAAAGGCAAAATTATCAGTGAATTCTCTAAGTCA GACAGGTGGGAGTCTGTATATGGAAGCTGGGAGTACATTGGATTTTGTAACTCCACAACCACCACAACAG CCTCCTGCCGCTAATCAGTTGATCACGCTTTCCAATCTGCATTTGTCTCTTTCTTCTTTGTTAGCAAACA ATGCAGTTACGAATCCTCCTACCAATCCTCCAGCGCAAGATTCTCATCCTGCAATCATTGGTAGCACAAC TGCTGGTTCTGTTACAATTAGTGGGCCTATCTTTTTTGAGGATTTGGATGATACAGCTTATGATAGGTAT GATTGGCTAGGTTCTAATCAAAAAATCGATGTCCTGAAATTACAGTTAGGGACTCAGCCCTCAGCTAATG CCCCATCAGATTTGACTCTAGGGAATGAGATGCCTAAGTATGGCTATCAAGGAAGCTGGAAGCTTGCGTG GGATCCTAATACAGCAAATAATGGTCCTTATACTCTGAAAGCTACATGGACTAAAACTGGGTATAATCCT GGGCCTGAGCGAGTAGCTTCTTTGGTTCCAAATAGTTTATGGGGATCCATTTTAGATATACGATCTGCGC ATTCAGCAATTCAAGCAAGTGTGGATGGGCGCTCTTATTGTCGAGGATTATGGGTTTCTGGAGTTTCGAA TTTCTTCTATCATGACCGCGATGCTTTAGGTCAGGGATATCGGTATATTAGTGGGGGTTATTCCTTAGGA GCAAACTCCTACTTTGGATCATCGATGTTTGGTCTAGCATTTACCGAAGTATTTGGTAGATCTAAAGATT ATGTAGTGTGTCGTTCCAATCATCATGCTTGCATAGGATCCGTTTATCTATCTACCAAACAAGCTTTATG TGGATCCTATTTGTTCGGAGATGCGTTTATCCGTGCTAGCTACGGGTTTGGGAACCAGCATATGAAAACC TCATACACATTTGCAGAGGAGAGCGATGTTCGTTGGGATAATAACTGTCTGGTTGGAGAGATTGGAGTGG GATTACCGATTGTGATTACTCCATCTAAGCTCTATTTGAATGAGTTGCGTCCTTTCGTGCAAGCTGAGTT TTCTTATGCCGATCATGAATCTTTTACAGAGGAAGGCGATCAAGCTCGGGCATTCAGGAGTGGACATCTC ATGAATCTATCAGTTCCTGTTGGAGTAAAATTTGATCGATGTTCTAGTACACACCCTAATAAATATAGCT TTATGGGGGCTTATATCTGTGATGCTTATCGCACCATCTCTGGGACTCAGACAACACTCCTATCCCATCA AGAGACATGGACAACAGATGCCTTTCATTTGGCAAGACATGGAGTCATAGTTAGAGGGTCTATGTATGCT TCTCTAACAAGCAATATAGAAGTATATGGCCATGGAAGATATGAGTATCGAGATACTTCTCGAGGTTATG GTTTGAGTGCAGGAAGTAAAGTCCGGTTCTAA >gi|3329345|gb|AAC68468.1| Putative Outer Membrane Protein F [Chlamydia trachomatis][SEQ ID NO:11] MIKRTSLSFACLSFFYLSTISILQANETDTLQFRRFTFSDREIQFVLDPASLITAQNIVLSNLQSNGTGA CTISGNTQTQIFSNSVNTTADSGGAFDMVTTSFTASDNANLLFCNNYCTHNKGGGAIRSGGPIRFLNNQD VLFYNNISAGAKYVGTGDHNEKNRGGALYATTITLTGNRTLAFINNMSGDCGGAISADTQISITDTVKGI LFENNHTLNHIPYTQAENMARGGAICSRRDLCSISNNSGPIVFNYNQGGKGGAISATRCVIDNNKERIIF SNNSSLGWSQSSSASNGGAIQTTQGFTLRNNKGSIYFDSNTATHAGGAINCGYIDIRDNGPVYFLNNSAA WGAAFNLSKPRSATNYIHTGTGDIVFNNNVVFTLDGNLLGKRKLFHINNNEITPYTLSLGAKKDTRIYFY DLFQWERVKENTSNNPPSPTSRNTITVNPETEFSGAVVFSYNQMSSDIRTLMGKEHNYIKEAPTTLKFGT LAIEDDAELEIFNIPFTQNPTSLLALGSGATLTVGKHGKLNITNLGVILPIILKEGKSPPCIRVNPQDMT QNTGTGQTPSSTSSISTPMIIFNGRLSIVDENYESVYDSMDLSRGKAEQLILSIETTNDGQLDSNWQSSL NTSLLSPPHYGYQGLWTPNWITTTYTITLNNNSSAPTSATSIAEQKKTSETFTPSNTTTASIPNIKASAG SGSGSASNSGEVTITKHTLVVNWAPVGYIVDPIRRGDLIANSLVHSGRNMTMGLRSLLPDNSWFALQGAA TTLFTKQQKRLSYHGYSSASKGYTVSSQASGAHGHKFLLSFSQSSDKMKEKETNNRLSSRYYLSALCFEH PMFDRIALIGAAACNYGTHNMRSFYGTKKSSKGKFHSTTLGASLRCELRDSMPLRSIMLTPFAQALFSRT EPASIRESGDLARLFTLEQAHTAVVSPIGIKGAYSSDTWPTLSWEMELAYQPTLYWKRPLLNTLLIQNNG SWVTTNTPLAKHSFYGRGSHSLKFSHLKLFANYQAEVATSTVSHYINAGGALVF >gi|3329342|gb|AE001360.1:c7571-4467[SEQ ID NO:12] ATGATTAAAAGAACTTCTCTATCCTTTGCTTGCCTCAGTTTTTTTTATCTTTCAACTATATCCATTTTGC AAGCTAATGAAACGGATACGCTACAGTTCCGGCGATTTACTTTTTCGGATAGAGAGATTCAGTTCGTCCT AGATCCCGCCTCTTTAATTACCGCCCAAAACATCGTTTTATCTAATTTACAGTCAAACGGAACCGGAGCC TGTACCATTTCAGGCAATACGCAAACTCAAATCTTTTCTAATTCCGTTAACACCACCGCAGATTCTGGTG GAGCCTTTGATATGGTTACTACCTCATTCACGGCCTCTGATAATGCTAATCTACTCTTCTGCAACAACTA CTGCACACATAATAAAGGCGGAGGAGCTATTCGTTCCGGAGGACCTATTCGATTCTTAAATAATCAAGAC GTGCTTTTTTATAATAACATATCGGCAGGGGCTAAATATGTTGGAACAGGAGATCACAACGAAAAAAATA GGGGCGGTGCGCTTTATGCAACTACTATCACTTTGACAGGGAATCGAACTCTTGCCTTTATTAACAATAT GTCTGGAGACTGCGGTGGAGCCATCTCTGCTGACACTCAAATATCAATAACTGATACCGTTAAAGGAATT TTATTTGAAAACAATCACACGCTCAATCATATACCGTACACGCAAGCTGAAAATATGGCACGAGGAGGAG CAATCTGTAGTAGAAGAGACTTGTGCTCAATCAGCAATAATTCTGGTCCCATAGTTTTTAACTATAACCA AGGCGGGAAAGGTGGAGCTATTAGCGCTACCCGATGTGTTATTGACAATAACAAAGAAAGAATCATCTTT TCAAACAATAGTTCCCTGGGATGGAGCCAATCTTCTTCTGCAAGTAACGGAGGAGCCATTCAAACGACAC AAGGATTTACTTTACGAAATAATAAAGGCTCTATCTACTTCGACAGCAACACTGCTACACACGCCGGGGG AGCCATTAACTGTGGTTACATTGACATCCGAGATAACGGACCCGTCTATTTTCTAAATAACTCTGCTGCC TGGGGAGCGGCCTTTAATTTATCGAAACCACGTTCAGCGACAAATTATATCCATACAGGGACAGGCGATA TTGTTTTTAATAATAACGTTGTCTTTACTCTTGACGGTAATTTATTAGGGAAACGGAAACTTTTTCATAT TAATAATAATGAGATAACACCATATACATTGTCTCTCGGCGCTAAAAAAGATACTCGTATCTATTTTTAT GATCTTTTCCAATGGGAGCGTGTTAAAGAAAATACTAGCAATAACCCACCATCTCCTACCAGTAGAAACA CCATTACCGTTAACCCGGAAACAGAGTTTTCTGGAGCTGTTGTGTTCTCCTACAATCAAATGTCTAGTGA CATACGAACTCTGATGGGTAAAGAACACAATTACATTAAAGAAGCCCCAACTACTTTAAAATTCGGAACG CTAGCCATAGAAGATGATGCAGAATTAGAAATCTTCAATATCCCGTTTACCCAAAATCCGACTAGCCTTC TTGCTTTAGGAAGCGGCGCTACGCTGACTGTTGGAAAGCACGGTAAGCTCAATATTACAAATCTTGGTGT TATTTTACCCATTATTCTCAAAGAGGGGAAGAGTCCGCCTTGTATTCGCGTCAACCCACAAGATATGACC CAAAATACTGGTACCGGCCAAACTCCATCAAGCACAAGTAGTATAAGCACTCCAATGATTATCTTTAATG GGCGCCTCTCAATTGTAGACGAAAATTATGAATCAGTCTACGACAGTATGGACCTCTCCAGAGGGAAAGC AGAACAACTAATTCTATCCATAGAAACCACTAATGATGGGCAATTAGACTCCAATTGGCAAAGTTCTCTG AATACTTCTCTACTCTCTCCTCCACACTATGGCTATCAAGGTCTATGGACTCCTAATTGGATAACAACAA CCTATACCATCACGCTTAATAATAATTCTTCAGCTCCAACATCTGCTACCTCCATCGCTGAGCAGAAAAA AACTAGTGAAACTTTTACTCCTAGTAACACAACTACAGCTAGTATCCCTAATATTAAAGCTTCCGCAGGA TCAGGCTCTGGATCGGCTTCCAATTCAGGAGAAGTTACGATTACCAAACATACCCTTGTTGTAAACTGGG CACCAGTCGGCTACATAGTAGATCCTATTCGTAGAGGAGATCTGATAGCCAATAGCTTAGTACATTCAGG AAGAAACATGACCATGGGCTTACGATCATTACTCCCGGATAACTCTTGGTTTGCTTTGCAAGGAGCTGCA ACAACATTATTTACAAAACAACAAAAACGTTTGAGTTATCATGGCTACTCTTCTGCATCAAAGGGGTATA CCGTCTCTTCTCAAGCATCAGGAGCTCATGGTCATAAGTTTCTTCTTTCCTTCTCCCAGTCATCTGATAA GATGAAAGAAAAAGAAACAAATAACCGCCTTTCTTCTCGTTACTATCTTTCTGCTTTATGTTTCGAACAT CCTATGTTTGATCGCATTGCTCTTATCGGAGCAGCAGCTTGCAATTATGGAACACATAACATGCGGAGTT TCTATGGAACTAAAAAATCTTCTAAAGGGAAATTTCACTCTACAACCTTAGGAGCTTCTCTTCGCTGTGA ACTACGCGATAGTATGCCTTTACGATCAATAATGCTCACCCCATTTGCTCAGGCTTTATTCTCTCGAACA GAACCAGCTTCTATCCGAGAAAGCGGTGATCTAGCTAGATTATTTACATTAGAGCAAGCCCATACTGCCG TTGTCTCTCCAATAGGAATCAAAGGAGCTTATTCTTCTGATACATGGCCAACACTCTCTTGGGAAATGGA ACTAGCTTACCAACCCACCCTCTACTGGAAACGTCCTCTACTCAACACACTATTAATCCAAAATAACGGT TCTTGGGTCACCACAAATACCCCATTAGCTAAACATTCCTTTTATGGGAGAGGTTCTCACTCCCTCAAAT TTTCTCATCTGAAACTATTTGCTAACTATCAAGCAGAAGTGGCTACTTCCACTGTCTCACACTACATCAA TGCAGGAGGAGCTCTGGTCTTTTAA >gi|3329344|gb|AAC68467.1| Putative Outer Membrane Protein E [Chlamydia trachomatis][SEQ ID NO:13] MKKAFFFFLIGNSLSGLAREVPSRIFLMPNSVPDPTKESLSNKISLTGDTHNLTNCYLDNLRYILAILQK TPNEGAAVTITDYLSFFDTQKEGIYFAKNLTPESGGAIGYASPNSPTVEIRDTIGPVIFENNTCCRLFTW RNPYAADKIREGGAIHAQNLYINHNHDVVGFMKNFSYVQGGAISTANTFVVSENQSCFLFMDNICIQTNT AGKGGAIYAGTSNSFESNNCDLFFINNACCAGGAIFSPICSLTGNRGNIVFYNNRCFKNVETASSEASDG GAIKVTTRLDVTGNRGRIFFSDNITKNYGGAIYAPVVTLVDNGPTYFINNIANNKGGAIYIDGTSNSKIS ADRHAIIFNENIVTNVTNANGTSTSANPPRRNAITVASSSGEILLGAGSSQNLIFYDPIEVSNAGVSVSF NKEADQTGSVVFSGATVNSADFHQRNLQTKTPAPLTLSNGFLCIEDHAQLTVNRFTQTGGVVSLGNGAVL SCYKNGTGDSASNASITLKHIGLNLSSILKSGAEIPLLWVEPTNNSNNYTADTAATFSLSDVKLSLIDDY GNSPYESTDLTHALSSQPMLSISEASDNQLQSENIDFSGLNVPHYGWQGLWTWGWAKTQDPEPASSATIT DPQKANRFHRTLLLTWLPAGYVPSPKHRSPLIANTLWGNMLLATESLKNSAELTPSGHPFWGITGGGLGM MVYQDPRENHPGFHMRSSGYSAGMIAGQTHTFSLKFSQTYTKLNERYAKNNVSSKNYSCQGEMLFSLQEG FLLTKLVGLYSYGDHNCHHFYTQGENLTSQGTFRSQTMGGAVFFDLPMKPFGSTHILTAPFLGALGIYSS LSHFTEVGAYPRSFSTKTPLINVLVPIGVKGSFMNATHRPQAWTVELAYQPVLYRQEPGIAAQLLASKGI WFGSGSPSSRHAMSYKISQQTQPLSWLTLHFQYHGFYSSSTFCNYLNGEIALRF >gi|3329342|gb|AE001360.1:c4464-1570[SEQ ID NO:14] ATGAAAAAAGCGTTTTTCTTTTTCCTTATCGGAAACTCCCTATCAGGACTAGCTAGAGAGGTTCCTTCTA GAATCTTTCTTATGCCCAACTCAGTTCCAGATCCTACGAAAGAGTCGCTATCAAATAAAATTAGTTTGAC AGGAGACACTCACAATCTCACTAACTGCTATCTCGATAACCTACGCTACATACTGGCTATTCTACAAAAA ACTCCCAATGAAGGAGCTGCTGTCACAATAACAGATTACCTAAGCTTTTTTGATACACAAAAAGAAGGTA TTTATTTTGCAAAAAATCTCACCCCTGAAAGTGGTGGTGCGATTGGTTATGCGAGTCCCAATTCTCCTAC CGTGGAGATTCGTGATACAATAGGTCCTGTAATCTTTGAAAATAATACTTGTTGCAGACTATTTACATGG AGAAATCCTTATGCTGCTGATAAAATAAGAGAAGGCGGAGCCATTCATGCTCAAAATCTTTACATAAATC ATAATCATGATGTGGTCGGATTTATGAAGAACTTTTCTTATGTCCAAGGAGGAGCCATTAGTACCGCTAA TACCTTTGTTGTGAGCGAGAATCAGTCTTGTTTTCTCTTTATGGACAACATCTGTATTCAAACTAATACA GCAGGAAAAGGTGGCGCTATCTATGCTGGAACGAGCAATTCTTTTGAGAGTAATAACTGCGATCTCTTCT TCATCAATAACGCCTGTTGTGCAGGAGGAGCGATCTTCTCCCCTATCTGTTCTCTAACAGGAAATCGTGG TAACATCGTTTTCTATAACAATCGCTGCTTTAAAAATGTAGAAACAGCTTCTTCAGAAGCTTCTGATGGA GGAGCAATTAAAGTAACTACTCGCCTAGATGTTACAGGCAATCGTGGTAGGATCTTTTTTAGTGACAATA TCACAAAAAATTATGGCGGAGCTATTTACGCTCCTGTAGTTACCCTAGTGGATAATGGCCCTACCTACTT TATAAACAATATCGCCAATAATAAGGGGGGCGCTATCTATATAGACGGAACCAGTAACTCCAAAATTTCT GCCGACCGCCATGCTATTATTTTTAATGAAAATATTGTGACTAATGTAACTAATGCAAATGGTACCAGTA CGTCAGCTAATCCTCCTAGAAGAAATGCAATAACAGTAGCAAGCTCCTCTGGTGAAATTCTATTAGGAGC AGGGAGTAGCCAAAATTTAATTTTTTATGATCCTATTGAAGTTAGCAATGCAGGGGTCTCTGTGTCCTTC AATAAGGAAGCTGATCAAACAGGCTCTGTAGTATTTTCAGGAGCTACTGTTAATTCTGCAGATTTTCATC AACGCAATTTACAAACAAAAACACCTGCACCCCTTACTCTCAGTAATGGTTTTCTATGTATCGAAGATCA TGCTCAGCTTACAGTGAATCGATTCACACAAACTGGGGGTGTTGTTTCTCTTGGGAATGGAGCAGTTCTG AGTTGCTATAAAAATGGTACAGGAGATTCTGCTAGCAATGCCTCTATAACACTGAAGCATATTGGATTGA ATCTTTCTTCCATTCTGAAAAGTGGTGCTGAGATTCCTTTATTGTGGGTAGAGCCTACAAATAACAGCAA TAACTATACAGCAGATACTGCAGCTACCTTTTCATTAAGTGATGTAAAACTCTCACTCATTGATGACTAC GGGAACTCTCCTTATGAATCCACAGATCTGACCCATGCTCTGTCATCACAGCCTATGCTATCTATTTCTG AAGCTAGCGATAACCAGCTACAATCAGAAAATATAGATTTTTCGGGACTAAATGTCCCTCATTATGGATG GCAAGGACTTTGGACTTGGGGCTGGGCAAAAACTCAAGATCCAGAACCAGCATCTTCAGCAACAATCACT GATCCACAAAAAGCCAATAGATTTCATAGAACCTTACTACTAACATGGCTTCCTGCCGGGTATGTTCCTA GCCCAAAACACAGAAGTCCCCTCATAGCTAACACCTTATGGGGGAATATGCTGCTTGCAACAGAAAGCTT AAAAAATAGTGCAGAGCTGACACCTAGTGGTCATCCTTTCTGGGGAATTACAGGAGGAGGACTAGGCATG ATGGTTTACCAAGATCCTCGAGAAAATCATCCTGGATTCCATATGCGCTCTTCCGGATACTCTGCGGGGA TGATAGCAGGGCAGACACACACCTTCTCATTGAAATTCAGTCAGACCTACACCAAACTCAATGAGCGTTA CGCAAAAAACAACGTATCTTCTAAAAATTACTCATGCCAAGGAGAAATGCTCTTCTCATTGCAAGAAGGT TTCTTGCTGACTAAATTAGTTGGGCTTTACAGCTATGGAGACCATAACTGTCACCATTTCTATACTCAAG GAGAAAATCTAACATCTCAAGGGACGTTCCGCAGTCAAACGATGGGAGGTGCTGTCTTTTTTGATCTCCC TATGAAACCCTTTGGATCAACGCATATACTGACAGCTCCCTTTTTAGGTGCTCTTGGTATTTATTCTAGC CTGTCTCACTTTACTGAGGTGGGAGCCTATCCGCGAAGCTTTTCTACAAAGACTCCTTTGATCAATGTCC TAGTCCCTATTGGAGTTAAAGGTAGCTTTATGAATGCTACCCACAGACCTCAAGCCTGGACTGTAGAATT GGCATACCAACCCGTTCTGTATAGACAAGAACCAGGGATCGCAGCCCAGCTCCTAGCCAGTAAGGGTATT TGGTTCGGTAGTGGAAGCCCCTCATCGCGTCATGCCATGTCCTATAAAATCTCACAGCAAACACAACCTT TGAGTTGGTTAACTCTCCATTTCCAGTATCATGGATTCTACTCCTCTTCAACCTTCTGTAATTATCTCAA TGGGGAAATTGCTCTGCGATTCTAG >gi|3329279|gb|AAC68408.1| Putative Outer Membrane Protein D [Chlamydia trachomatis][SEQ ID NO:15] MSSEKDIKSTCSKFSLSVVAAILASVSGLASCVDLHAGGQSVNELVYVGPQAVLLLDQIRDLFVGSKDSQ AEGQYRLIVGDPSSFQEKDADTLPGKVEQSTLFSVTNPVVFQGVDQQDQVSSQGLICSFTSSNLDSPRDG ESFLGIAFVGDSSKAGITLTDVKASLSGAALYSTEDLIFEKIKGGLEFASCSSLEQGGACAAQSILIHDC QGLQVKHCTTAVNAEGSSANDHLGFGGGAFFVTGSLSGEKSLYMPAGDMVVANCDGAISFEGNSANFANG GAIAASGKVLFVANDKKTSFIENRALSGGAIAASSDIAFQNCAELVFKGNCAIGTEDKGSLGGGAISSLG TVLLQGNHGITCDKNESASQGGAIFGKNCQISDNEGPVVFRDSTACLGGGAIAAQEIVSIQNNQAGISFE GGKASFGGGIACGSFSSAGGASVLGTIDISKNLGAISFSRTLCTTSDLGQMEYQGGGALFGENISLSENA GVLTFKDNIVKTFASNGKILGGGAILATGKVEITNNSEGISFTGNARAPQALPTQEEFPLFSKKEGRPLS SGYSGGGAILGREVAILHNAAVVFEQNRLQCSEEEATLLGCCGGGAVHGMDSTSIVGNSSVRFGNNYAMG QGVSGGALLSKTVQLAGNGSVDFSRNIASLGGGALQASEGNCELVDNGYVLFRDNRGRVYGGAISCLRGD VVISGNKGRVEFKDNIATRLYVEETVEKVEEVEPAPEQKDNNELSFLGRAEQSFITAANQALFASEDGDL SPESSISSEELAKRRECAGGAIFAKRVRIVDNQEAVVFSNNFSDIYGGAIFTGSLREEDKLDGQIPEVLI SGNAGDVVFSGNSSKRDEHLPHTGGGAICTQNLTISQNTGNVLFYNNVACSGGAVRIEDHGNVLLEAFGG DIVFKGNSSFRAQGSDAIYFAGKESHITALNATEGHAIVFHDALVFENLEERKSAEVLLINSRENPGYTG SIRFLEAESKVPQCIHVQQGSLELLNGATLCSYGFKQDAGAKLVLAAGAKLKILDSGTPVQQGHAISKPE AEIESSSEPEGAHSLWIAKNAQTTVPMVDIHTISVDLASFSSSQQEGTVEAPQVIVPGGSYVRSGELNLE LVNTTGTGYENHALLKNEAKVPLMSFVASGDEASAEISNLSVSDLQIHVVTPEIEEDTYGHMGDWSEAKI QDGTLVISWNPTGYRLDPQKAGALVFNALWEEGAVLSALKNARFAHNLTAQRMEFDYSTNVWGFAFGGFR TLSAENLVAIDGYKGAYGGASAGVDIQLMEDFVLGVSGAAFLGKMDSQKFDAEVSRKGVVGSVYTGFLAG SWFFKGQYSLGETQNDMKTRYGVLGESSASWTSRGVLADALVEYRSLVGPVRPTFYALHFNPYVEVSYAS MKFPGFTEQGREARSFEDASLTNITIPLGMKFELAFIKGQFSEVNSLGISYAWEAYRKVEGGAVQLLEAG FDWEGAPMDLPRQELRVALENNTEWSSYFSTVLGLTAFCGGFTSTDSKLGYEANTGLRLIF >gi|3329271|gb|AE001353.1:9710-14305[SEQ ID NO:16] ATGAGTTCCGAGAAAGATATAAAAAGCACCTGTTCTAAGTTTTCTTTGTCTGTAGTAGCAGCTATCCTTG CCTCTGTTAGCGGGTTAGCTAGTTGCGTAGATCTTCATGCTGGAGGACAGTCTGTAAATGAGCTGGTATA TGTAGGCCCTCAAGCGGTTTTATTGTTAGACCAAATTCGAGATCTATTCGTTGGGTCTAAAGATAGTCAG GCTGAAGGACAGTATAGGTTAATTGTAGGAGATCCAAGTTCTTTCCAAGAGAAAGATGCGGATACTCTTC CCGGGAAGGTAGAGCAAAGTACTTTGTTCTCAGTAACCAATCCCGTGGTTTTCCAAGGTGTGGACCAACA GGATCAAGTCTCTTCCCAAGGGTTAATTTGTAGTTTTACGAGCAGCAACCTTGATTCTCCTCGTGACGGA GAATCTTTTTTAGGTATTGCTTTTGTTGGGGATAGTAGTAAGGCTGGAATCACATTAACTGACGTGAAAG CTTCTTTGTCTGGAGCGGCTTTATATTCTACAGAAGATCTTATCTTTGAAAAGATTAAGGGTGGATTGGA ATTTGCATCATGTTCTTCTCTAGAACAGGGGGGAGCTTGTGCAGCTCAAAGTATTTTGATTCATGATTGT CAAGGATTGCAGGTTAAACACTGTACTACAGCCGTGAATGCTGAGGGGTCTAGTGCGAATGATCATCTTG GATTTGGAGGAGGCGCTTTCTTTGTTACGGGTTCTCTTTCTGGAGAGAAAAGTCTCTATATGCCTGCAGG AGATATGGTAGTTGCGAATTGTGATGGGGCTATATCTTTTGAAGGAAACAGCGCGAACTTTGCTAATGGA GGAGCGATTGCTGCCTCTGGGAAAGTGCTTTTTGTCGCTAATGATAAAAAGACTTCTTTTATAGAGAACC GAGCTTTGTCTGGAGGAGCGATTGCAGCCTCTTCTGATATTGCCTTTCAAAACTGCGCAGAACTAGTTTT CAAAGGCAATTGTGCAATTGGAACAGAGGATAAAGGTTCTTTAGGTGGAGGGGCTATATCTTCTCTAGGC ACCGTTCTTTTGCAAGGGAATCACGGGATAACTTGTGATAAGAATGAGTCTGCTTCGCAAGGAGGCGCCA TTTTTGGCAAAAATTGTCAGATTTCTGACAACGAGGGGCCAGTGGTTTTCAGAGATAGTACAGCTTGCTT AGGAGGAGGCGCTATTGCAGCTCAAGAAATTGTTTCTATTCAGAACAATCAGGCTGGGATTTCCTTCGAG GGAGGTAAGGCTAGTTTCGGAGGAGGTATTGCGTGTGGATCTTTTTCTTCCGCAGGTGGTGCTTCTGTTT TAGGGACCATTGATATTTCGAAGAATTTAGGCGCGATTTCGTTCTCTCGTACTTTATGTACGACCTCAGA TTTAGGACAAATGGAGTACCAGGGAGGAGGAGCTCTATTTGGTGAAAATATTTCTCTTTCTGAGAATGCT GGTGTGCTCACCTTTAAAGACAACATTGTGAAGACTTTTGCTTCGAATGGGAAAATTCTGGGAGGAGGAG CGATTTTAGCTACTGGTAAGGTGGAAATTACTAATAATTCCGAAGGAATTTCTTTTACAGGAAATGCGAG AGCTCCACAAGCTCTTCCAACTCAAGAGGAGTTTCCTTTATTCAGCAAAAAAGAAGGGCGACCACTCTCT TCAGGATATTCTGGGGGAGGAGCGATTTTAGGAAGAGAAGTAGCTATTCTCCACAACGCTGCAGTAGTAT TTGAGCAAAATCGTTTGCAGTGCAGCGAAGAAGAAGCGACATTATTAGGTTGTTGTGGAGGAGGCGCTGT TCATGGGATGGATAGCACTTCGATTGTTGGCAACTCTTCAGTAAGATTTGGTAATAATTACGCAATGGGA CAAGGAGTCTCAGGAGGAGCTCTTTTATCTAAAACAGTGCAGTTAGCTGGGAATGGAAGCGTCGATTTTT CTCGAAATATTGCTAGTTTGGGAGGAGGAGCTCTTCAAGCTTCTGAAGGAAATTGTGAGCTAGTTGATAA CGGCTATGTGCTATTCAGAGATAATCGAGGGAGGGTTTATGGGGGTGCTATTTCTTGCTTACGTGGAGAT GTAGTCATTTCTGGAAACAAGGGTAGAGTTGAATTTAAAGACAACATAGCAACACGTCTTTATGTGGAAG AAACTGTAGAAAAGGTTGAAGAGGTAGAGCCAGCTCCTGAGCAAAAAGACAATAATGAGCTTTCTTTCTT AGGGAGAGCAGAACAGAGTTTTATTACTGCAGCTAATCAAGCTCTTTTCGCATCTGAAGATGGGGATTTA TCACCTGAGTCATCCATTTCTTCTGAAGAACTTGCGAAAAGAAGAGAGTGTGCTGGAGGAGCTATTTTTG CAAAACGGGTTCGTATTGTAGATAACCAAGAGGCCGTTGTATTCTCGAATAACTTCTCTGATATTTATGG CGGCGCCATTTTTACAGGTTCTCTTCGAGAAGAGGATAAGTTAGATGGGCAAATCCCTGAAGTCTTGATC TCAGGCAATGCAGGGGATGTTGTTTTTTCCGGAAATTCCTCGAAGCGTGATGAGCATCTTCCTCATACAG GTGGGGGAGCCATTTGTACTCAAAATTTGACGATTTCTCAGAATACAGGGAATGTTCTGTTTTATAACAA CGTGGCCTGTTCGGGAGGAGCTGTTCGTATAGAGGATCATGGTAATGTTCTTTTAGAAGCTTTTGGAGGA GATATTGTTTTTAAAGGAAATTCTTCTTTCAGAGCACAAGGATCCGATGCTATCTATTTTGCAGGTAAAG AATCGCATATTACAGCCCTGAATGCTACGGAAGGACATGCTATTGTTTTCCACGACGCATTAGTTTTTGA AAATCTAGAAGAAAGGAAATCTGCTGAAGTATTGTTAATCAATAGTCGAGAAAATCCAGGTTACACTGGA TCTATTCGATTTTTAGAAGCAGAAAGTAAAGTTCCTCAATGTATTCATGTACAACAAGGAAGCCTTGAGT TGCTAAATGGAGCCACATTATGTAGTTATGGTTTTAAACAAGATGCTGGAGCTAAGTTGGTATTGGCTGC TGGAGCTAAACTGAAGATTTTAGATTCAGGAACTCCTGTACAACAAGGGCATGCTATCAGTAAACCTGAA GCAGAAATCGAGTCATCTTCTGAACCAGAGGGTGCACATTCTCTTTGGATTGCGAAGAATGCTCAAACAA CAGTTCCTATGGTTGATATCCATACTATTTCTGTAGATTTAGCCTCCTTCTCTTCTAGTCAACAGGAGGG GACAGTAGAAGCTCCTCAGGTTATTGTTCCTGGAGGAAGTTATGTTCGATCTGGAGAGCTTAATTTGGAG TTAGTTAACACAACAGGTACTGGTTATGAAAATCATGCTTTATTGAAGAATGAGGCTAAAGTTCCATTGA TGTCTTTCGTTGCTTCTGGTGATGAAGCTTCAGCCGAAATCAGTAACTTGTCGGTTTCTGATTTACAGAT TCATGTAGTAACTCCAGAGATTGAAGAAGACACATACGGCCATATGGGAGATTGGTCTGAGGCTAAAATT CAAGATGGAACTCTTGTCATTAGTTGGAATCCTACTGGATATCGATTAGATCCTCAAAAAGCAGGGGCTT TAGTATTTAATGCATTATGGGAAGAAGGGGCTGTCTTGTCTGCTCTGAAAAATGCACGCTTTGCTCATAA TCTCACTGCTCAGCGTATGGAATTCGATTATTCTACAAATGTGTGGGGATTCGCCTTTGGTGGTTTCCGA ACTCTATCTGCAGAGAATCTGGTTGCTATTGATGGATACAAAGGAGCTTATGGTGGTGCTTCTGCTGGAG TCGATATTCAATTGATGGAAGATTTTGTTCTAGGAGTTAGTGGAGCTGCTTTCCTAGGTAAAATGGATAG TCAGAAGTTTGATGCGGAGGTTTCTCGGAAGGGAGTTGTTGGTTCTGTATATACAGGATTTTTAGCTGGA TCCTGGTTCTTCAAAGGACAATATAGCCTTGGAGAAACACAGAACGATATGAAAACGCGTTATGGAGTAC TAGGAGAGTCGAGTGCTTCTTGGACATCTCGAGGAGTACTGGCAGATGCTTTAGTTGAATACCGAAGTTT AGTTGGTCCTGTGAGACCTACTTTTTATGCTTTGCATTTCAATCCTTATGTCGAAGTATCTTATGCTTCT ATGAAATTCCCTGGCTTTACAGAACAAGGAAGAGAAGCGCGTTCTTTTGAAGACGCTTCCCTTACCAATA TCACCATTCCTTTAGGGATGAAGTTTGAATTGGCGTTCATAAAAGGACAGTTTTCAGAGGTGAACTCTTT GGGAATAAGTTATGCATGGGAAGCTTATCGAAAAGTAGAAGGAGGCGCGGTGCAGCTTTTAGAAGCTGGG TTTGATTGGGAGGGAGCTCCAATGGATCTTCCTAGACAGGAGCTGCGTGTCGCTCTGGAAAATAATACGG AATGGAGTTCTTACTTCAGCACAGTCTTAGGATTAACAGCTTTTTGTGGAGGATTTACTTCTACAGATAG TAAACTAGGATATGAGGCGAATACTGGATTGCGATTGATCTTTTAA >gi|3329169|gb|AAC68308.1| Outer Membrane Protein Analog [Chlamydia trachomatis][SEQ ID NO:17] MSSKLVNYLRLTFLSFLGIASTSLDAMPAGNPAFPVIPGINIEQKNACSFDLCNSYDVLSALSGNLKLCF CGDYIFSEEAQVKDVPVVTSVTTAGVGPSPDITSTTKTRNFDLVNCNLNTNCVAVAFSLPDRSLSAIPLF DVSFEVKVGGLKQYYRLPMNAYRDFTSEPLNSESEVTDGMIEVQSNYGFVWDVSLKKVIWKDGVSFVGVG ADYRHASCPIDYIIANSQANPEVFIADSDGKLNFKEWSVCVGLTTYVNDYVLPYLAFSIGSVSRQAPDDS FKKLEDRFTNLKFKVRKITSSHRGNICIGATNYVADNFFYNVEGRWGSQRAVNVSGGFQF >gi|3329166|gb|AE001342.1:c4638-3616[SEQ ID NO:18] ATGAGTAGCAAGCTAGTGAACTATCTCCGTTTGACTTTCCTATCTTTTTTAGGGATCGCATCTACTTCAT TAGACGCTATGCCTGCGGGGAATCCGGCGTTTCCAGTCATCCCGGGGATTAATATTGAACAGAAAAATGC CTGTTCTTTCGATTTATGTAATTCTTATGATGTACTATCCGCACTGTCCGGTAACCTGAAGCTCTGCTTC TGCGGAGATTATATCTTTTCAGAAGAAGCTCAGGTAAAAGATGTCCCTGTCGTTACCTCTGTGACAACAG CTGGGGTTGGTCCTTCTCCTGATATTACTTCGACAACCAAAACGCGAAATTTCGATCTCGTGAACTGTAA TCTCAATACAAACTGTGTAGCTGTAGCTTTTTCCCTTCCTGATCGTTCGCTGAGCGCGATTCCTCTGTTT GATGTGAGTTTCGAAGTGAAAGTAGGAGGACTGAAACAATACTACCGCCTTCCCATGAATGCCTATCGAG ACTTCACCTCGGAACCTCTCAATTCTGAATCAGAAGTTACGGACGGGATGATTGAAGTACAGTCCAATTA CGGATTTGTTTGGGATGTTAGCTTGAAAAAAGTCATATGGAAAGATGGCGTTTCCTTTGTAGGCGTCGGT GCAGACTATCGCCATGCTTCTTGCCCTATTGACTACATCATTGCAAACAGTCAAGCTAATCCAGAAGTAT TCATCGCTGACTCGGATGGGAAACTGAACTTCAAGGAGTGGAGTGTCTGCGTAGGTCTTACTACCTATGT GAATGACTACGTTCTTCCTTACTTAGCGTTTTCTATAGGGAGTGTTTCTCGCCAAGCTCCGGACGACAGC TTCAAAAAATTAGAAGATCGCTTCACTAACCTCAAATTTAAAGTTCGTAAAATTACCAGCTCTCATCGTG GAAACATCTGCATCGGAGCGACAAACTATGTCGCCGATAACTTCTTCTACAACGTAGAAGGAAGATGGGG AAGCCAGCGCGCTGTGAACGTCTCCGGAGGATTCCAATTCTAA >gi|3328866|gb|AAC68034.1| Sulfite Reductase [Chlamydia trachomatis][SEQ ID NO:19] MSLFSKFKAQWMFLHSRELCSSTSDIGNTCSDPVFQVLCNPVRSEISYKVGDSLGVFPTNPSILVDSVLD ALQYGPRSPVVSRHADSVLPLHEFLTSYVDLDKIPKSLRPFFPGDLDDTWSLAEAILVYQPRIPFEEFIR SAMPLLPRFYSIASSPTCSHGKLELLVRCVSFQGKTQLRYGLCSAFLCKDLQEGESFRGFIQPTRHFTLE QKNFGKPLIMIGAGTGIAPYKGFLQHRIYHQDVGSNILFFGERFEKSNFYYRDFLQELIVSGKLQLFTAF SRDSESKLYVQNVIEQQKELIQEVYEQEAFFFVCGKKILGTEVKRALEQILGPKAVRELIAQKRLVSDVY >gi|3328863|gb|AE001317:c2573-1521[SEQ ID NO:20] ATGTCTTTATTTTCTAAATTCAAAGCTCAGTGGATGTTTTTACATTCACGTGAGCTTTGTTCTTCCACAT CGGATATTGGGAATACTTGTTCGGATCCTGTTTTTCAGGTTTTATGTAATCCGGTTCGTTCTGAGATTTC CTATAAAGTTGGGGATTCTTTGGGGGTATTCCCAACAAATCCTTCCATATTAGTCGATTCAGTTCTAGAT GCTTTACAGTATGGCCCCAGGTCTCCTGTCGTATCTCGGCATGCAGATTCTGTTCTCCCTCTTCACGAAT TTCTTACTAGTTACGTAGACTTAGATAAAATTCCAAAATCGTTAAGACCTTTTTTCCCAGGGGATTTAGA CGATACCTGGTCTTTAGCTGAAGCTATTTTGGTTTACCAGCCGCGTATTCCTTTTGAAGAGTTTATTCGG AGTGCGATGCCTTTATTGCCTCGATTTTATTCTATAGCTTCTTCTCCAACATGTTCTCATGGGAAGCTAG AGTTGCTCGTGCGCTGTGTTAGTTTCCAAGGTAAAACGCAGCTGCGCTATGGATTATGTTCGGCTTTTTT ATGTAAGGACTTACAAGAGGGAGAGTCTTTTCGTGGGTTTATACAACCGACGCGGCATTTTACTTTGGAG CAGAAAAATTTTGGGAAACCTTTAATTATGATCGGAGCAGGGACAGGTATCGCTCCGTACAAAGGGTTCT TACAACATCGAATATACCATCAGGACGTAGGCTCCAATATTCTATTCTTTGGAGAGCGTTTTGAGAAAAG TAACTTCTATTACCGGGATTTTCTCCAGGAGCTGATCGTTTCAGGAAAACTCCAGTTATTCACAGCCTTT TCCAGAGATTCCGAGTCTAAATTGTATGTTCAGAATGTTATAGAGCAACAAAAAGAACTTATACAAGAAG TCTACGAACAAGAAGCTTTCTTTTTTGTTTGTGGGAAAAAAATCCTTGGTACGGAAGTTAAACGTGCTTT AGAGCAGATATTAGGTCCTAAGGCGGTACGAGAGCTGATTGCACAGAAGAGACTAGTTTCAGACGTATAC TAA >gi|3328843|gb|AAC68011.1| Putative outer membrane protein C [Chlamydia trachomatis][SEQ ID NO:21] MKFMSATAVFAAALSSVTEASSIQDQIKNTDCNVSKLGYSTSQAFTDMMLADNTEYRAADSVSFYDFSTS SRLPRKHLSSSSEASPTTEGVSSSSSGETDEKTEEELDNGGIIYAREKLTISESQDSLSNQSIELHDNSI FFGEGEVIFDHRVALKNGGAIYGEKEVVFENIKSLLVEVNIAVEKGGSVYAKERVSLENVTEATFSSNGG EQGGGGIYSEQDMLISDCNNVHFQGNAAGATAVKQCLDEEMIVLLAECVDSLSEDTLDSTPETEQTESNG NQDGSSETEDTQVSESPESTPSPDDVLGKGGGIYTEKSLTITGITGTIDFVSNIATDSGAGVFTKENLSC TNTNSLQFLKNSAGQHGGGAYVTQTMSVTNTTSESITTPPLIGEVIFSENTAKGHGGGICTNKLSLSNLK TVTLTKNSAKESGGAIFTDLASIPITDTPESSTPSSSSPASTPEVVASAKINRFFASTAKPAAPSLTEAE SDQTDQTETSDTNSDIDVSIENILNVAINQNTSAKKGGAIYGKKAKLSRINNLELSGNSSQDVGGGLCLT ESVEFDAIGSLLSHYNSAAKEGGAIHSKTVTLSNLKSTFTFADNTVKAIVESTPEAPEEIPPVEGEESTA TEDPNSNTEGSSANTNLEGSQGDTADTGTGDVNNESQDTSDTGNAESEEQLQDSTQSNEENTLPNSNIDQ SNENTDESSDSHTEEITDESVSSSSESGSSTPQDGGAASSGAPSGDQSISANACLAKSYAASTDSSPVSN SSGSEEPVTSSSDSDVTASSDNPDSSSSGDSAGDSEEPTEPEAGSTTETLTLIGGGAIYGETVKIENFSG QGIFSGNKAIDNTTEGSSSKSDVLGGAVYAKTLFNLDSGSSRRTVTFSGNTVSSQSTTGQVAGGAIYSPT VTIATPVVFSKNSATNNANNTTDTQRKDTFGGAIGATSAVSLSGGAHFLENVADLGSAIGLVPGTQNTET VKLESGSYYFEKNKALKRATIYAPVVSIKAYTATFNQNRSLEEGSAIYFTKEASIESLGSVLFTGNLVTL TLSTTTEGTPATTSGDVTKYGAAIFGQIASSNGSQTDNLPLKLIASGGNICFRNNEYRPTSSDTGTSTFC SIAGDVKLTMQAAKGKTISFFDAIRTSTKKTGTQATAYDTLDINKSEDSETVNSAFTGTILFSSELHENK SYIPQNVVLHSGSLVLKPNTELHVISFEQKEGSSLVMTPGSVLSNQTVADGALVINNMTIDLSSVEKNGI AEGNIFTPPELRIIDTTTGGSGGTPSTDSESNQNSDDTEEQNNNDASNQGESANGSSSPAVAAAHTSRTR NFAAAATATPTTTPTATTTTSNQVILGGEIKLIDPNGTFFQNPALRSDQQISLLVLPTDSSKMQAQKIVL TGDIAPQKGYTGTLTLDPDQLQNGTISVLWKFDSYRQWAYVPRDNHFYANSILGSQMLMVTVKQGLLNDK MNLARFEEVSYNNLWISGLGTMLSQVGTPTSEEFTYYSRGASVALDAKPAHDVIVGAAFSKMIGKTKSLK RENNYTHKGSEYSYQASVYGGKPFHFVINKKTEKSLPLLLQGVISYGYIKHDTVTHYPTIRERNKGEWED LGWLTALRVSSVLRTPAQGDTKRITVYGELEYSSIRQKQFTETEYDPRYFDNCTYRNLAIPMGLAFEGEL SGNDILMYNRFSVAYMLSIYRNSPTCKYQVLSSGEGGEIICGVPTRNSARGEYSTQLYLGPLWTLYGSYT IEADAHTLAHMMNCGARMTF >gi|3328842|gb|AE001315.1:120-5432[SEQ ID NO:22] ATGAAATTTATGTCAGCTACTGCTGTATTTGCTGCAGCACTCTCCTCCGTTACTGAGGCGAGCTCGATCC AAGATCAAATAAAGAATACCGACTGCAATGTTAGCAAATTAGGATATTCAACTTCTCAAGCATTTACTGA TATGATGCTAGCAGACAACACAGAGTATCGAGCTGCTGATAGTGTTTCATTCTATGACTTTTCGACATCT TCCAGATTACCTAGAAAACATCTTAGTAGTAGTAGTGAAGCTTCTCCAACGACAGAAGGAGTGTCTTCAT CTTCATCTGGAGAAACTGATGAGAAAACAGAAGAAGAACTAGACAATGGCGGAATCATTTATGCTAGAGA GAAACTAACTATCTCAGAATCTCAGGACTCTCTCTCTAATCAAAGCATAGAACTCCATGACAATAGTATT TTCTTCGGAGAAGGTGAAGTTATCTTTGATCACAGAGTTGCCCTCAAAAACGGAGGAGCTATTTATGGAG AGAAAGAGGTAGTCTTTGAAAACATAAAATCTCTACTAGTAGAAGTAAATATCGCGGTCGAGAAAGGGGG TAGCGTCTATGCAAAAGAACGAGTATCTTTAGAAAATGTTACCGAAGCAACCTTCTCCTCCAATGGTGGG GAACAAGGTGGTGGTGGAATCTATTCAGAACAGGATATGTTAATCAGTGATTGCAACAATGTACATTTCC AAGGGAATGCTGCAGGAGCAACAGCAGTAAAACAATGTCTGGATGAAGAAATGATCGTATTGCTCGCAGA ATGCGTTGATAGCTTATCCGAAGATACACTGGATAGCACTCCAGAAACGGAACAGACTGAGTCAAATGGA AATCAAGACGGTTCGTCTGAAACAGAAGATACACAAGTATCAGAATCACCAGAATCAACTCCTAGCCCCG ACGATGTTTTAGGTAAAGGTGGTGGTATCTATACAGAAAAATCTTTGACCATCACTGGAATTACACGGAC TATAGATTTTGTCAGTAACATAGCTACCGATTCTGGAGCAGGTGTATTCACTAAAGAAAACTTGTCTTGC ACCAACACGAATAGCCTACAGTTTTTGAAAAACTCGGCAGGTCAACATGGAGGAGGAGCCTACGTTACTC AAACCATGTCTGTTACTAATACAACTAGTGAAAGTATAACTACTCCCCCTCTCATAGGACAAGTGATTTT CTCTGAAAATACAGCTAAAGGGCACGGTGGTGGTATCTGCACTAACAAACTTTCTTTATCTAATTTAAAA ACGGTGACTCTCACTAAAAACTCTGCAAAGGAGTCTGGAGGAGCTATTTTTACAGATCTGGCGTCTATAC CAATAACAGATACCCCAGAATCTTCTACCCCCTCTTCCTCCTCGCCTGCAAGCACTCCTGAAGTAGTTGC TTCTGCTAAAATAAATCGATTCTTTGCCTCTACGGCAAAACCGGCAGCCCCTTCTCTAACAGAGGCTGAG TCTGATCAAACGGATCAAACAGAAACTTCTGATACTAATAGCGATATAGACGTGTCGATTGAGAACATTT TGAATGTCGCTATCAATCAAAACACTTCTGCGAAAAAAGGAGGGGCTATTTACGGGAAAAAAGCTAAACT TTCCCGTATTAACAATCTTGAACTTTCAGGGAATTCATCCCAGGATGTAGGAGGAGGTCTCTGTTTAACT GAAAGCGTAGAATTTGATGCAATTGGATCGCTCTTATCCCACTATAACTCTGCTGCTAAAGAAGGTGGGG CTATTCATTCTAAAACGGTTACTCTATCTAACCTCAAGTCTACCTTCACTTTTGCAGATAACACTGTTAA AGCAATAGTAGAAAGCACTCCTGAAGCTCCAGAAGAGATTCCTCCAGTAGAAGGAGAAGAGTCTACAGCA ACAGAAGATCCAAATTCTAATACAGAAGGAAGTTCGGCTAACACTAACCTTGAAGGATCTCAAGGGGATA CTGCTGATACAGGGACTGGTGATGTTAACAATGAGTCTCAAGACACATCAGATACTGGAAACGCTGAATC TGAAGAACAACTACAAGATTCTACACAATCTAATGAAGAAAATACCCTTCCCAATAGTAATATTGATCAA TCTAACGAAAACACAGACGAATCATCTGATAGCCACACTGAGGAAATAACTGACGAGAGTGTCTCATCGT CCTCTGAAAGTGGATCATCTACTCCTCAAGATGGAGGAGCAGCTTCTTCAGGGGCTCCCTCAGGAGATCA ATCTATCTCTGCAAACGCTTGTTTAGCTAAAAGCTATGCTGCGAGTACTGATAGCTCCCCCGTATCTAAT TCTTCAGGTTCAGAAGAGCCTGTCACTTCTTCTTCAGATTCAGACGTTACTGCATCTTCTGATAATCCAG ACTCTTCCTCATCTGGAGATAGCGCTGGAGACTCTGAAGAACCGACTGAGCCAGAAGCTGGTTCTACAAC AGAAACTCTTACTTTAATAGGAGGAGGTGCTATCTATGGAGAAACTGTTAAGATTGAGAACTTCTCTGGC CAAGGAATATTTTCTGGAAACAAAGCTATCGATAACACCACAGAAGGCTCCTCTTCCAAATCTGACGTCC TCGGAGGTGCGGTCTATGCTAAAACATTGTTTAATCTCGATAGCGGGAGCTCTAGACGAACTGTCACCTT CTCCGGGAATACTGTCTCTTCTCAATCTACAACAGGTCAGGTTGCTGGAGGAGCTATCTACTCTCCTACT GTAACCATTGCTACTCCTGTAGTATTTTCTAAAAACTCTGCAACAAACAATGCTAATAACACTACAGATA CTCAGAGAAAAGACACCTTTGGAGGAGCTATCGGAGCTACTTCTGCTGTTTCTCTATCAGGAGGGGCTCA TTTCTTAGAAAACGTTGCTGACCTCGGATCTGCTATTGGGTTGGTGCCAGGCACACAAAATACAGAAACA GTGAAATTAGAGTCTGGCTCCTACTACTTTGAAAAAAATAAAGCTTTAAAACGAGCTACTATTTACGCAC CTGTCGTTTCCATTAAAGCCTATACTGCGACATTTAACCAAAACAGATCTCTAGAAGAAGGAAGCGCGAT TTACTTTACAAAAGAAGCATCTATTGAGTCTTTAGGCTCTGTTCTCTTCACAGGAAACTTAGTAACCCTA ACGCTAAGCACAACTACAGAAGGCACACCAGCCACAACCTCAGGAGATGTAACAAAATATGGTGCTGCTA TCTTTGGACAAATAGCAAGCTCAAACGGATCTCAGACGGATAACCTTCCCCTGAAACTCATTGCTTCAGG AGGAAATATTTGTTTCCGAAACAATGAATACCGTCCTACTTCTTCTGATACCGGAACCTCTACTTTCTGT AGTATTGCGGGAGATGTTAAATTAACCATGCAAGCTGCAAAAGGGAAAACGATCAGTTTCTTTGATGCAA TCCGGACCTCTACTAAGAAAACAGGTACACAGGCAACTGCCTACGATACTCTCGATATTAATAAATCTGA GGATTCAGAAACTGTAAACTCTGCGTTTACAGGAACGATTCTGTTCTCCTCTGAATTACATGAAAATAAA TCCTATATTCCACAAAACGTAGTTCTACACAGTGGATCTCTTGTATTGAAGCCAAATACCGAGCTTCATG TTATTTCTTTTGAGCAGAAAGAAGGCTCTTCTCTCGTTATGACACCTGGATCTGTTCTTTCGAACCAGAC TGTTGCTGATGGAGCTTTGGTCATAAATAACATGACCATTGATTTATCCAGCGTAGAGAAAAATGGTATT GCTGAAGGAAATATCTTTACTCCTCCAGAATTGAGAATCATAGACACTACTACAGGTGGAAGCGGTGGAA CCCCATCTACAGATAGTGAAAGTAACCAGAATAGTGATGATACCGAGGAGCAAAATAATAATGACGCCTC GAATCAAGGAGAAAGCGCGAATGGATCGTCTTCTCCTGCAGTAGCTGCTGCACACACATCTCGTACAAGA AACTTTGCCGCTGCAGCTACAGCCACACCTACGACAACACCAACGGCTACAACTACAACAAGCAACCAAG TAATCCTAGGAGGAGAAATTAAACTCATCGATCCTAATGGGACCTTCTTCCAGAACCCTGCATTAAGATC CGACCAACAAATCTCCTTGTTAGTGCTCCCTACAGACTCATCAAAAATGCAAGCTCAGAAAATAGTACTG ACGGGTGATATTGCTCCTCAGAAAGGATATACAGGAACACTCACTCTGGATCCTGATCAACTACAAAATG GAACGATCTCAGTGCTCTGGAAATTTGACTCTTATAGACAATGGGCTTATGTACCTAGAGACAATCATTT CTATGCGAACTCGATTCTGGGATCTCAAATGTTAATGGTCACAGTCAAACAAGGCTTGCTCAACGATAAA ATGAATCTAGCTCGCTTTGAGGAAGTTAGCTATAACAACCTGTGGATATCAGGACTAGGAACGATGCTAT CGCAAGTAGGAACACCTACTTCTGAAGAATTCACTTATTACAGCAGAGGAGCTTCTGTTGCCTTAGATGC TAAACCAGCCCATGATGTGATTGTTGGAGCTGCATTTAGTAAGATGATCGGGAAAACAAAATCCTTGAAA AGAGAGAATAACTACACTCACAAAGGATCCGAATATTCTTACCAAGCATCGGTATACGGAGGCAAACCAT TCCACTTTGTAATCAATAAAAAAACGGAAAAATCGCTACCGCTATTGTTACAAGGAGTCATCTCTTACGG ATATATCAAACATGATACAGTGACTCACTATCCAACGATCCGTGAACGAAACAAAGGAGAATGGGAAGAC TTAGGATGGCTGACAGCTCTCCGTGTCTCCTCTGTCTTAAGAACTCCTGCACAAGGGGATACTAAACGTA TCACTGTTTACGGAGAATTGGAATACTCCAGTATCCGTCAGAAACAATTCACAGAAACAGAATACGATCC TCGTTACTTCGACAACTGCACCTATAGAAACTTAGCAATTCCTATGGGGTTAGCATTCGAAGGAGAGCTC TCTGGTAACGATATTTTGATGTACAACAGATTCTCTGTAGCATACATGCTATCAATCTATCGAAATTCTC CAACATGCAAATACCAAGTGCTCTCTTCAGGAGAAGGCGGAGAAATTATTTGTGGAGTACCGACAAGAAA CTCAGCTCGCGGAGAATACAGCACGCAGCTGTACCTGGGACCTTTGTGGACTCTGTATGGATCCTACACG ATAGAAGCAGACGCACATACACTAGCTCATATGATGAACTGCGGTGCTCGTATGACATTCTAA >gi|3328815|gb|AAC67986.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO:23] MMKPLRFGYFFCAIYFTLLQAAFAKEPNSCPDCQNNWKEVTHTDQLPENIIHADDACYHSGYVQALIDMH FLDSCCQVIVENQTAYLFSLPTDDVTRNAIINLIKDLPFIHSVEICQASYQTCHHQGPHGKTSLPEQRSF CTKVCGKEAIWLPQNTILFSPLVADPRQATNSAGIRFNDEVLGKRVGSATFGGDFIFLRLFDISRFHGDM DIGLQGAVFSVFDLDHPEACMVNSDFFVAALCNFAVNKWSYRFRLWHLSSHLGDEFILANQLPPKKRYNR SDEAVDFFASFRYTPQIRVYGGIGYIISRDLTFPEDPLYFEGGIELRPFGLREDNLHAQPVFAMHFRFWE EHDFSIDQTYIVGMEWSKFQDVGRKVRAVLEYHQGFSHEGQFVREECDYYGFRLSYGF >gi|3328812|gb|AE001312.1:2790-4016[SEQ ID NO:24] ATGATGAAACCTCTACGTTTCGGTTATTTCTTTTGCGCAATCTATTTTACTTTGTTACAGGCAGCGTTTG CTAAAGAACCGAATTCTTGTCCCGACTGCCAGAATAATTGGAAAGAAGTCACCCACACGGATCAACTCCC AGAAAACATCATTCATGCTGATGATGCTTGTTATCACTCTGGTTATGTACAGGCTCTCATTGATATGCAT TTCTTAGATAGCTGCTGCCAGGTCATCGTTGAAAACCAAACTGCTTACTTATTTTCTCTTCCTACAGATG ATGTTACGCGCAACGCCATTATCAACCTAATTAAAGACCTTCCATTCATTCACTCCGTAGAAATCTGCCA AGCATCCTATCAAACCTGTCATCATCAAGGCCCTCATGGAAAGACTTCTCTTCCAGAACAACGTTCTTTC TGTACAAAGGTCTGTGGAAAAGAAGCTATTTGGTTACCACAGAATACCATCCTATTCTCGCCTCTTGTAG CAGATCCTAGACAAGCAACTAATAGTGCAGGTATCCGTTTTAACGACGAAGTCTTAGGAAAACGTGTTGG CTCTGCTACCTTCGGTGGAGATTTCATCTTCTTACGATTATTTGATATCTCCCGATTCCATGGAGACATG GATATTGGTCTCCAAGGAGCTGTATTCTCTGTTTTCGACCTGGATCATCCAGAAGCTTGCATGGTCAACT CTGACTTTTTTGTCGCCGCTTTGTGCAACTTTGCAGTGAACAAATGGAGCTACCGCTTCAGACTATGGCA TCTTTCTTCTCATCTTGGCGACGAATTTATTCTTGCCAACCAGTTACCTCCTAAAAAACGTTATAATCGA AGCGATGAAGCCGTCGATTTCTTTGCTTCTTTTCGTTACACTCCACAGATCCGTGTTTATGGAGGTATTG GGTATATCATTAGTCGAGATTTAACATTCCCTGAAGATCCTCTTTACTTTGAAGGAGGTATCGAACTACG TCCTTTCGGATTACGGGAAGACAACCTTCATGCCCAACCCGTCTTTGCTATGCATTTTCGCTTTTGGGAA GAGCATGACTTTTCTATAGACCAAACTTATATAGTAGGCATGGAGTGGTCCAAATTCCAGGATGTAGGGA GAAAAGTGCGCGCTGTATTGGAATACCACCAAGGTTTCTCCCACGAAGGACAATTTGTCCGAGAAGAATG CGATTATTATGGCTTTCGATTAAGTTATGGCTTCTAG >gi|3328651|gb|AAC67834.1| Omp85 Analog [Chlamydia trachomatis][SEQ ID NO:25] MLGIRKKTILQLAVLLLLTFSRSSFCSTSEGRMVVESITITTQGENTQNKRAIPKIKTKQGTLFSQADFD EDLRTLSKDFDRVEPIVEFRNGQAVISLILTAKPVIREINISGNEAIPTHKILKTLELYKNDLFDRELFF KNFDALRTLYLKRGYYDSQLSYSHNHNEKEGFIDISIEIKEGRHGRIKKLTISGITRTEASDLGDIVLTK QYSTTTSWFTGAGVYHPDMVEQDLFAITNYFQNKGYADAKVSKEVSTDAKGNITLLIVVDKGPLYTLGHV HIEGFTALSKRLLDKQLLVGPNSLYCPDKIWTGAQKIRSAYARYGYVNTNVDVSFSAHPTLPVYDVTYRV SEGSPYKIGLIKIKGNTHTKHDVILHETSLFPGDTFDRLKLEGTETRLRNTGYFKSVSVYTVRSQLDPLD SNDLYRDVFIEVKETETGNLGLFLGFSSIDHLFGGAEIAESNFDLFGARNFLKKGFKSLRGGGEYLFLKA NLGDKVTDYTVKWTKPHFLNTPWILGVELDKSINKALSKDYSVDTYGGNISTTYILNDKLKYGMYYRGSQ TSLSLRKKTSSSNRPGPDLDSNKGFVSAAGLNVLYDSIDNPRKPTMGIRSSLNFELSGLGGTYQFTKLTA SGSIYRLLTKKGVLKVRAEAKFIKPFGTTTAQGIPVSERFFLGGETTVRGYKPFIIGPKFSPTEPQGGLS SLLLTEEFQYPLISQPCINAFVFLDSGFIGIEEYTIRLKDLCSSAGFGLRFDMMNNVPIMLGWGWPFRPT EILNNEKIDVSQRFFFALGGVF >gi|3328646|gb|AE001297.1:4000-6378[SEQ ID NO:26] ATGCTTGGAATACGCAAAAAAACGATTCTGCAACTCGCTGTTTTACTGTTGCTCACCTTTTCACGAAGTT CTTTCTGTTCAACTTCAGAAGGACGTATGGTCGTAGAGTCTATCACCATTACGACTCAAGGAGAGAATAC TCAAAATAAACGAGCTATTCCTAAAATAAAAACAAAGCAGGGGACGTTGTTCTCTCAAGCAGATTTTGAT GAAGATCTAAGAACACTTTCGAAAGATTTTGATCGAGTAGAGCCTATCGTAGAGTTTCGTAATGGACAAG CTGTGATCTCTCTGATTCTGACGGCAAAACCTGTTATCAGAGAGATCAATATTTCAGGAAATGAAGCTAT CCCCACTCATAAAATTCTGAAAACTTTAGAGCTTTATAAAAATGATCTTTTTGATCGGGAATTATTCTTT AAAAATTTTGATGCGCTAAGAACTCTTTATTTGAAACGAGGGTACTACGATTCTCAACTCTCCTATTCTC ATAATCATAATGAGAAAGAGGGCTTTATCGATATTTCCATCGAGATTAAAGAAGGACGTCACGGTCGCAT AAAAAAATTAACGATTTCGGGAATTACGCGAACAGAAGCATCAGACTTAGGTGACATTGTTTTAACTAAA CAATACTCCACAACAACGAGCTGGTTCACTGGTGCCGGAGTGTATCATCCGGACATGGTAGAGCAAGACT TATTTGCTATCACAAATTACTTCCAAAATAAAGGATATGCTGATGCTAAAGTAAGCAAAGAGGTCTCTAC AGATGCTAAAGGAAACATTACTTTGCTTATCGTTGTAGACAAAGGACCTTTATACACATTAGGTCACGTA CATATAGAAGGATTCACAGCGTTATCCAAAAGACTGCTCGATAAACAACTATTGGTTGGACCTAACTCCT TATATTGCCCAGATAAAATTTGGACTGGAGCACAAAAGATTCGTAGCGCATACGCTAGATATGGCTACGT GAACACTAACGTTGATGTCTCCTTCTCAGCGCACCCCACTCTACCTGTTTACGATGTTACCTATCGAGTG AGTGAAGGATCTCCCTACAAAATCGGGTTAATTAAAATCAAAGGGAACACTCATACTAAGCATGATGTGA TTTTGCATGAGACTAGTCTTTTCCCTGGAGACACTTTTGATAGATTAAAACTGGAAGGTACAGAGACTCG TTTACGCAACACCGGCTACTTTAAAAGTGTAAGTGTCTATACGGTTCGTTCCCAATTAGATCCTCTTGAT TCTAACGACCTTTATCGAGATGTTTTTATTGAAGTCAAAGAGACTGAAACAGGAAATCTTGGGCTATTCT TAGGATTCAGCTCCATTGACCATTTATTTGGAGGGGCAGAAATTGCAGAAAGCAACTTTGATTTATTTGG AGCCCGAAACTTTCTCAAAAAAGGATTCAAATCTTTAAGAGGTGGTGGAGAATACCTCTTCCTAAAAGCT AATTTAGGAGATAAGGTCACCGATTACACTGTTAAATGGACGAAACCACACTTCTTAAATACCCCTTGGA TTCTTGGAGTAGAATTAGATAAATCAATTAATAAAGCTTTATCAAAAGACTACTCTGTGGATACCTATGG AGGGAATATCAGTACCACCTACATTCTTAACGATAAGTTAAAATATGGGATGTATTACCGTGGTAGCCAA ACAAGCTTAAGTTTGCGCAAAAAAACGTCCAGCTCTAATAGACCTGGACCAGATTTAGATAGTAATAAAG GATTTGTTTCCGCAGCGGGACTCAATGTTCTCTATGATTCTATTGATAATCCTAGAAAACCTACTATGGG AATCCGCAGCTCCTTAAACTTTGAATTATCTGGTTTAGGCGGAACTTACCAATTTACTAAACTAACAGCT AGTGGTTCTATCTATCGCTTATTAACTAAAAAAGGTGTTTTGAAAGTCCGTGCAGAAGCTAAGTTTATCA AACCTTTCGGAACAACAACTGCACAAGGCATTCCTGTCAGCGAACGGTTCTTCTTAGGAGGTGAAACCAC TGTTCGCGGTTACAAACCTTTTATTATTGGACCGAAATTTTCTCCTACTGAACCACAAGGAGGCTTGTCT TCCCTACTATTAACAGAAGAATTTCAATATCCTTTGATTTCTCAACCTTGCATTAATGCCTTTGTATTTC TAGATTCCGGATTCATTGGGATAGAAGAGTACACTATTCGCCTGAAAGACCTTTGCAGTAGCGCCGGATT TGGTCTACGCTTTGATATGATGAATAATGTGCCAATTATGCTAGGCTGGGGTTGGCCGTTCCGCCCAACA GAAATCCTCAATAATGAAAAAATTGATGTATCTCAAAGATTCTTTTTTGCCTTGGGAGGAGTATTCTAG >gi|3328587|gb|AAC67774.1| CMP-2-keto-3-deoxyoctulosonic acid synthetase [Chlamydia trachomatis][SEQ ID NO:27] MFAFLTSKKVGILPSRWGSSRFPGKPLAKILGKILVQRSYENALSSQSLDCVVVATDDQRIFDHVVEFGG LCVMTSTSCANGTERVEEVVSRHFPQAEIVVNIQGDEPCLSPTVIDGLVSTLENNPAADMVTSVTETTDP EAILTDHKVKCVFDKNGKALYFSRSAIPHNFKHPTPIYLHIGVYAFRKAFLSEYVKIPPSSLSLAEDLEQ LRVLEIGRSIYVHVVQNATGPSVDYPEDITKVEQYLLCLSKASF >gi|3328586|gb|AE001292.1:216-980[SEQ ID NO:28] GTGTTTGCGTTTTTAACCAGCAAAAAAGTCGGCATTCTCCCCTCTAGATGGGGAAGCTCCCGCTTCCCCG GAAAACCTCTAGCAAAAATTCTAGGGAAAACCCTTGTTCAAAGATCCTATGAAAATGCCTTAAGCAGTCA ATCTCTAGATTGCGTTGTTGTGGCAACAGATGATCAACGAATTTTTGACCATGTCGTTGAATTTGGGGGG CTCTGTGTCATGACTAGCACATCTTGCGCTAACGGAACTGAGCGAGTAGAAGAGGTTGTGTCTCGACATT TTCCTCAAGCAGAGATTGTTGTGAACATCCAAGGAGACGAGCCCTGTTTATCTCCTACCGTCATAGATGG GCTTGTGAGCACGCTAGAGAACAATCCTGCTGCAGATATGGTCACATCTGTTACAGAAACAACAGACCCC GAAGCGATATTGACAGATCACAAAGTGAAGTGTGTTTTCGATAAGAATGGCAAAGCTCTTTACTTTAGCA GAAGCGCTATTCCTCACAACTTTAAACACCCAACGCCTATTTATCTGCATATTGGTGTTTATGCTTTTAG AAAAGCTTTTCTAAGTGAATATGTTAAAATTCCTCCTTCCTCGTTAAGCCTAGCCGAAGATCTTGAACAA TTACGAGTATTAGAAATAGGTCGTTCTATCTACGTTCATGTCGTTCAGAATGCAACGGGCCCTTCTGTTG ATTATCCCGAAGATATAACCAAAGTGGAGCAGTATTTATTATGTCTTTCAAAAGCATCTTTTTGA >gi|3329039|gb|AAC68197.1| Thio:disulfide Interchange Protein [Chlamydia trachomatis][SEQ ID NO:29] MIRQWYGFFLCLLFSYTSCFGVEENSGRATPTVELVSESEQAVEGEVLRIGVLIAIPEGEHIYWKNPGKL GMPLRISWDLPSGCRLLEEHWPTPEIFEEDGVVYFGYKHSTMVVADIRVSKEIETRPLEIKAQVEWLSCG ASCLPGSSSRVLVIPIDQGPLIPNSKETFTFSRALAAQPRPLDAAIKISYQPDGLDVFVPAGKADRATQA WFIAENTRDFAYAQEVPLEQATTYIWKLKHPEGNMPKGIGLSGILIFKDDAGKVVASYQVEENQVEQLSA LSWRFLSILLMAFIGGILLNIMPCVLPLITLKVFSLIKSAADHHSSSVIGGIGFTLGAIVSFWGLAFCAF LLKVLGQNIGWGFQLQEPMFVAVLIIVFFLFALSSLGVFEMGIICLSLGKKLQEEGGASVRKNQIWGAFF NGMLTTLVTTPCTGPFLGSVFGLVMAVSFVKQLAIFTAIGLGMASPYLLFASFPKMLAILPKPGPWMSTF KQLTGFMLLATATWLIWIFGVETSATAVTILLVGLWLAAVGAWILGRWGTLVSPRNQRLLASVVFIFCIL SSLVITSIGVRYFDENVPPAHSSDWQSFSPEKLADLREKGIPVFVNFTAKWCLTCQLNKPLLHANMQAFA AKGVVTLEADWTKKDPKITEELARLGRASVPSYVYYPAGNKAPLILPERLSQSALEEMVFSQ >gi|3329034|gb|AE001330.1:c6695-4617[SEQ ID NO:30] ATGATTCGGCAATGGTATGGATTTTTTCTTTGCTTGCTGTTCAGCTATACGTCTTGTTTTGGTGTAGAAG AAAATAGTGGAAGAGCTACGCCTACAGTAGAACTTGTTAGTGAAAGCGAACAAGCTGTTGAAGGAGAAGT GCTTCGTATCGGAGTATTGATTGCTATTCCAGAAGGAGAGCATATCTACTGGAAAAATCCAGGGAAGCTT GGAATGCCTTTGCGCATTTCTTGGGATTTGCCATCAGGATGTAGGTTGCTGGAGGAACATTGGCCGACTC CAGAGATTTTCGAAGAGGATGGGGTTGTTTATTTTGGTTATAAACATTCTACAATGGTGGTTGCGGATAT TCGCGTTTCTAAAGAGATAGAAACGCGTCCATTGGAGATAAAAGCGCAAGTTGAATGGTTGTCTTGCGGT GCATCTTGTCTCCCAGGTTCTTCGTCAAGGGTTCTTGTGATTCCTATAGATCAGGGGCCGTTAATTCCTA ATAGTAAAGAGACATTCACTTTTTCCCGTGCGTTAGCGGCTCAACCTCGACCTTTGGATGCTGCCATAAA GATTTCTTATCAGCCTGATGGCTTAGATGTTTTTGTGCCAGCAGGGAAAGCGGATCGGGCAACCCAGGCA TGGTTCATTGCTGAAAACACGCGAGATTTTGCTTATGCTCAAGAGGTTCCTCTTGAGCAAGCGACTACGT ACATATGGAAGTTGAAACATCCTGAAGGAAATATGCCTAAGGGTATTGGGTTGTCGGGGATTCTTATATT CAAGGATGATGCAGGGAAAGTAGTCGCTTCGTATCAAGTAGAAGAGAATCAAGTCGAACAGCTTTCGGCA TTGAGCTGGAGGTTTCTCTCTATTCTTCTTATGGCTTTCATTGGTGGAATCTTATTAAACATCATGCCCT GTGTATTGCCTCTGATTACTTTGAAAGTATTTAGTTTAATTAAATCGGCGGCAGATCACCATTCTTCCTC TGTGATTGGAGGGATTGGGTTTACTTTAGGGGCTATTGTAAGCTTTTGGGGACTCGCTTTTTGTGCGTTT TTGTTAAAGGTTTTAGGGCAAAATATTGGATGGGGATTCCAGCTTCAAGAACCCATGTTTGTTGCCGTTT TAATTATTGTCTTCTTCTTATTTGCTCTGAGTTCGTTAGGCGTTTTTGAGATGGGAATAATTTGTCTGAG CCTAGGGAAAAAATTGCAAGAAGAGGGAGGGGCATCGGTAAGGAAGAATCAGATCTGGGGAGCTTTTTTC AATGGGATGTTGACTACCCTGGTTACAACTCCTTGCACTGGGCCTTTTCTTGGCTCTGTATTTGGATTAG TTATGGCAGTGTCTTTTGTTAAGCAGCTGGCAATTTTTACTGCTATAGGATTAGGAATGGCAAGTCCCTA TCTATTATTTGCTTCTTTTCCGAAGATGCTAGCCATTTTACCTAAACCTGGTCCTTGGATGAGTACGTTT AAACAGTTGACTGGGTTTATGTTGCTTGCTACTGCAACTTGGCTTATCTGGATTTTTGGGGTAGAGACGA GTGCAACCGCTGTAACTATTCTTCTTGTAGGATTGTGGTTGGCTGCTGTAGGTGCATGGATTCTAGGGAG ATGGGGAACCCTTGTATCTCCGCGTAATCAGCGGCTTCTTGCTTCCGTTGTATTCATTTTCTGTATTTTA AGTTCCTTAGTGATTACCTCTATAGGTGTCCGTTATTTTGATGAGAACGTCCCTCCTGCACATAGCTCTG ATTGGCAATCTTTTTCTCCCGAAAAGCTAGCTGATTTACGCGAAAAAGGGATTCCAGTTTTTGTAAATTT CACTGCAAAGTGGTGTTTAACGTGTCAACTCAATAAGCCTCTTCTTCATGCCAATATGCAAGCTTTTGCT GCTAAGGGCGTAGTTACTTTAGAAGCAGATTGGACGAAAAAAGATCCAAAAATTACAGAAGAACTCGCTC GTTTAGGCCGAGCCAGTGTACCTTCTTATGTGTATTACCCTGCGGGGAACAAAGCTCCGCTTATTCTTCC AGAAAGATTATCGCAATCTGCTTTGGAAGAGATGGTTTTTTCTCAGTAG >gi|3329000|gb|AAC68161.1| Yop proteins translocation lipoprotein J [Chlamydia trachomatis][SEQ ID NO:31] MFRYTLSRSLFFILALFFCSACDSRSMITHGLSGRDANEIVVLLVSKGVAAQKVPQAASSTGGSGEQLWD ISVPAAQITEALAILNQAGLPRMKGTSLLDLFAKQGLVPSEMQEKIRYQEGLSEQMATTIRKMDGIVDAS VQISFSPEEEDQRPLTASVYIKHRGVLDNPNSIMVSKIKRLVASAVPGLCPENVSVVSDRASYSDITING PWGLSDEMNYVSVWGIILAKHSLTKFRLVFYFLILLLFILSCGLLWVIWKTHTLISALGGTKGFFDPAPY SQLSFTQNKPAPKETPGAAEGAEAQTASEQPSKENAEKQEENNEDA >gi|3328999|gb|AE001327.1:84-1064[SEQ ID NO:32] ATGTTTCGTTATACTCTTTCTCGATCCTTATTTTTCATTTTGGCTCTTTTCTTCTGCTCGGCTTGTGATA GTCGTTCCATGATTACACACGGCTTGTCAGGACGTGATGCTAATGAAATCGTAGTGCTTCTAGTCAGTAA AGGGGTCGCTGCACAGAAAGTTCCCCAAGCAGCGTCCTCAACAGGAGGATCTGGAGAACAACTCTGGGAT ATTTCGGTTCCTGCAGCACAAATTACAGAGGCTCTAGCTATTCTGAACCAAGCTGGGCTTCCAAGAATGA AAGGAACCAGCCTTCTTGATCTATTCGCTAAACAAGGGCTGGTCCCTTCTGAAATGCAAGAAAAAATCCG CTACCAAGAAGGTCTTTCAGAACAAATGGCTACGACCATTAGAAAGATGGACGGTATCGTCGATGCGAGC GTACAGATTTCCTTTTCTCCTGAAGAAGAAGATCAACGGCCGCTAACAGCCTCTGTATATATCAAACACA GAGGGGTATTAGACAACCCTAACAGTATTATGGTGTCTAAGATTAAACGTTTAGTTGCGAGTGCTGTCCC AGGACTATGTCCCGAGAACGTTTCCGTAGTCAGTGACCGAGCTTCTTATAGTGACATTACTATTAATGGC CCTTGGGGACTCTCCGATGAAATGAATTATGTTTCTGTATGGGGGATCATTCTAGCTAAGCATTCCCTTA CTAAATTCCGCCTTGTTTTCTATTTCTTAATTCTCCTTCTCTTCATTCTTTCCTGTGGGCTACTCTGGGT CATTTGGAAAACACACACACTGATTTCTGCTCTGGGTGGAACAAAAGGATTCTTTGATCCTGCTCCTTAC TCACAGCTCTCTTTCACTCAGAATAAGCCAGCTCCAAAAGAAACTCCTGGAGCAGCAGAAGGTGCAGAAG CGCAAACCGCTTCGGAACAACCCTCTAAAGAAAACGCAGAAAAACAAGAAGAGAATAACGAGGACGCTTA A >gi|3328905|gb|AAC68071.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO:33] MEKRGVIVHILVCLLTIFGTFSLPAFGAHFLAEEEQFYMDRFVFSGQYPDMETMEIHAERKKRVQFDVTG SFPKLESVVYKGSFGLLRSKIKGECPELSSVNLSCTSCRMDLDFRGEWKKNASIYIRNEQEPITIMLPKD IGVVVYTQVDMNSKVVAEGSLIKRGRGFWKKTFRNSLVGESPVTLTFHVETRNGGVIFLR >gi|3328891|gb|AE001320.1:c11104-10502[SEQ ID NO:34] ATGGAGAAGAGAGGCGTTATTGTGCATATACTAGTTTGTTTGTTGACAATCTTCGGAACGTTCAGTTTAC CCGCTTTCGGCGCGCATTTTCTCGCGGAAGAAGAGCAGTTTTATATGGATCGGTTTGTTTTCTCTGGGCA GTATCCAGATATGGAAACTATGGAAATCCATGCAGAAAGAAAAAAACGTGTACAATTTGATGTGACGGGA AGCTTCCCTAAGTTGGAGAGCGTGGTTTATAAGGGATCTTTTGGATTGCTGCGTTCGAAAATAAAGGGAG AGTGTCCAGAACTGTCTTCTGTAAATCTTTCTTGTACCTCCTGCAGAATGGATTTAGATTTTCGAGGGGA GTGGAAAAAGAATGCGTCTATTTATATTCGTAATGAGCAAGAGCCAATTACAATTATGTTGCCTAAAGAC ATTGGTGTAGTTGTCTATACGCAGGTTGATATGAATAGTAAAGTAGTTGCAGAGGGATCACTAATCAAGA GAGGAAGAGGTTTTTGGAAGAAAACTTTTCGGAATTCTTTGGTAGGAGAATCCCCTGTGACGCTAACTTT TCATGTAGAGACTCGTAATGGAGGAGTTATTTTTCTCCGTTAG >gi|3328884|gb|AAC68051.1| Phosphatidate Cytidylytransferase [Chlamydia trachomatis][SEQ ID NO:35] MFDSDHNSIFQSDLCQRLVVHSILLTFLVILLCTSLYPSSAFIVGLLSSACAALGTYEMGAMVRIKFPFS FTRYSALGSAIFIALTCLTARCKMCFPEHIDLLPWFFLFFWTIRLVFKSRHYKLGPIGSTGLALFCMLYV SVPIRLFLHILYGFVHTDTPFVGIWWAIFLIATTKSSDIFGYFFGKAFGKKRIAPVISPNKTVVGFIAGC CGSILVSLLFYSHLPKAFADQIAVPWILIALGTVLGVSGFFGDIIESTFKRDAQIKNSSDLESIGGMLDV LDSLLLSTPIVYAILLITQNRTFLG >gi|3328881|gb|AE001319.1:1804-2721[SEQ ID NO:36] ATGTTCGATTCGGATCATAATTCCATTTTTCAAAGCGATTTGTGTCAGCGTCTGGTCGTCCACTCGATTC TTCTTACTTTCCTTGTCATTCTTCTCTGTACATCTTTATATCCCAGCTCAGCCTTTATTGTAGGGCTTCT TTCCTCCGCTTGCGCAGCTCTAGGAACATATGAGATGGGGGCTATGGTTAGAATCAAGTTTCCATTTTCT TTCACACGCTATAGTGCATTAGGATCCGCTATTTTCATTGCTCTGACCTGTCTTACAGCTCGTTGTAAAA TGTGTTTTCCAGAGCATATAGACCTACTTCCTTGGTTCTTTCTCTTCTTTTGGACGATTCGCTTAGTATT TAAAAGTCGCCATTATAAACTTGGTCCCATAGGCTCAACTGGGCTCGCGTTGTTTTGTATGCTTTATGTA TCAGTCCCTATCCGCTTGTTCCTCCACATTTTGTATGGGTTTGTGCATACCGATACTCCATTTGTAGGAA TTTGGTGGGCGATTTTTCTTATCGCTACAACAAAAAGCTCTGATATTTTTGGTTACTTCTTTGGAAAAGC TTTTGGGAAAAAACGCATTGCACCAGTCATTAGCCCGAACAAAACAGTAGTAGGCTTCATTGCTGGTTGC TGTGGATCTATCTTGGTTAGCCTTCTTTTCTACTCCCATCTTCCTAAAGCCTTTGCTGATCAGATTGCGG TGCCTTGGATCCTCATTGCTTTAGGTACTGTGTTGGGCGTTAGTGGATTCTTTGGAGACATTATCGAATC TACGTTCAAACGGGATGCACAGATCAAGAACAGCAGTGATCTGGAGTCTATCGGAGGAATGCTAGATGTG CTAGACTCCTTGCTTCTTTCGACTCCTATCGTTTACGCTATCCTCCTTATCACTCAAAATAGGACATTTT TAGGATGA >gi|3328855|gb|AAC68022.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO:37] MRRSVCYVTPSVARAGQISTWRFEYSSANFLPEGTLLKFDLGIDGRPIDWEIPSTDLSQPCNTIYLETPS EDIVAAKAVYAPGGYIPTFEFTLPCDVEAGDTFSIILGSSPNFPQEDSSGNGAQLFTQRRKPFSLYVDPS GKGSFEDPDIFTMDIRGNVLKNIRIFAPSYVIKNKRFDITVRFEDEFGNLTNFSPEETHIELSYEHLREN LNWQLFIPETGFVILPNLYFNEPGIYRIQLRNQATKEVFTSAPIKCFAETSSHLLWGLLHGESERVDSEG NIESCLRYFRDDCALNFFATSSFEIQDGLTPETIKTINQTVADFNEEDRFIALSGAQYLSEEPGEGIREV LLMKEPKSPGKHKECKLFPLSKLYKQSTSHELISIPSFTASKKFGYNFNNFHPEFERVVEIYNAWGCSER TEAEGNPFPIKGSIDSENPEGTVLSALKRNLRFGFVAGGLDDRNLYNHFFDSDQQQYSPGLTAVICNKYS RDSLLEALYQRQCYATTGQRIIVNFQITSAPMGSELSTAIKPGLVINRHISGYVAGTAKIASIEIIRNED ILHTFHPDGNNFEYEYDDLSPFAQVTLKDPQNGAPFAFYYLRVTQENGAMAWSSPIWIDLN >gi|3328850|gb|AE001316.1:4105-5970[SEQ ID NO:38] ATGCGCAGATCTGTTTGTTACGTTACTCCTTCAGTTGCTAGGGCTGGTCAAATTTCTACCTGGCGTTTCG AATATTCTTCAGCTAATTTCCTTCCCGAAGGCACATTGCTAAAATTTGACCTGGGAATAGACGGACGCCC TATAGACTGGGAGATTCCTTCTACAGATCTTTCTCAACCATGTAATACAATTTATTTAGAAACGCCTTCC GAGGATATTGTGGCTGCAAAAGCTGTGTATGCTCCCGGAGGCTATATCCCTACTTTCGAATTTACTCTCC CTTGTGATGTGGAAGCTGGGGACACTTTCTCTATTATTCTTGGCTCCTCTCCCAACTTCCCTCAAGAGGA CTCTTCAGGTAATGGTGCTCAATTATTTACTCAACGCCGTAAACCTTTCTCTCTTTATGTTGACCCATCA GGGAAAGGATCTTTTGAAGATCCCGATATCTTCACAATGGATATCAGAGGAAATGTATTAAAAAATATCC GGATTTTTGCTCCTTCTTATGTGATCAAAAACAAACGCTTTGATATTACAGTGCGCTTCGAAGATGAATT TGGGAACTTAACCAATTTCTCCCCAGAAGAGACCCATATCGAGCTTTCGTACGAACATCTTCGCGAAAAC CTCAATTGGCAATTGTTCATCCCTGAAACAGGCTTTGTGATCCTTCCAAACCTGTATTTCAATGAACCAG GTATTTATCGTATTCAACTACGCAATCAAGCAACAAAAGAGGTCTTTACATCAGCGCCTATCAAATGTTT TGCAGAAACCTCCTCTCATCTTTTGTGGGGGCTTCTACATGGAGAATCTGAACGTGTCGACTCTGAAGGT AATATCGAGTCTTGCTTGCGTTATTTTCGTGATGACTGCGCGTTAAACTTTTTTGCAACATCCTCTTTCG AAATTCAAGATGGCCTGACCCCAGAAACCATTAAAACCATTAACCAAACCGTTGCTGATTTTAATGAAGA AGATCGTTTCATTGCCTTATCCGGAGCACAGTACCTTTCTGAAGAGCCTGGCGAGGGAATTCGTGAAGTA TTGCTGATGAAGGAACCCAAATCCCCAGGGAAACATAAAGAATGCAAACTATTTCCTTTATCTAAGCTAT ATAAGCAATCAACTAGTCATGAGTTAATCTCAATCCCCAGCTTCACTGCTTCAAAGAAATTTGGATACAA TTTTAATAATTTCCATCCTGAATTTGAAAGAGTTGTTGAAATTTATAATGCCTGGGGATGCTCTGAAAGA ACTGAAGCTGAAGGAAACCCTTTCCCTATTAAAGGTTCTATCGACTCAGAAAATCCAGAGGGAACTGTTC TATCTGCTTTAAAGAGAAACCTGCGTTTTGGATTCGTAGCCGGTGGTCTTGATGATAGAAATCTATACAA TCACTTTTTTGATTCCGATCAACAGCAATACTCCCCTGGATTAACAGCTGTGATCTGCAATAAATATTCT CGGGATTCCTTACTCGAGGCATTATACCAACGACAATGCTATGCTACAACCGGCCAAAGAATTATCGTGA ATTTCCAGATTACATCTGCTCCTATGGGCTCCGAACTCTCCACAGCCATTAAACCAGGGCTCGTGATCAA TAGACATATTTCGGGATATGTAGCAGGAACTGCCAAGATTGCGTCGATCGAAATCATCCGCAATGAGGAT ATTCTCCATACCTTCCACCCAGATGGAAATAACTTTGAGTATGAGTACGACGATCTCTCTCCTTTTGCAC AAGTCACTCTAAAAGATCCTCAAAATGGAGCTCCTTTTGCTTTTTACTACTTACGAGTCACTCAAGAGAA TGGAGCTATGGCTTGGAGCTCTCCTATTTGGATAGATCTTAACTAA >gi|3328772|gb|AAC67946.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO:39] MKRFFPLFIGVLLAHTLPSEGLSHQQAVQKKISYLSHFKGITGIMDVEDGVLHIHDDLRLQANKAYVENR TDCGIKIVAHGNVMVNYRGKILICDYLEYYEDTDSCLLTNGRCSLYPWFIGGSTITISPSSIIIHKGYIS TSEGPQKHICLSGDYLKYSSDSVLSMGPSRLSICNTPVLLLPQISIMPMEIPKPPITFRGGSGGFLGSYL GVSYSPISKKHCSTTLFLDGFFKHGIGLGYNMRFSSQENPSNAINIKSYYAHRLAIDSSGAKDRYRLHGD FTFSKERAHLAGEFHLSDSWETVVDIFPNNFSLKNTGPTEVSLSWRDNNLFGKMTSSVKVNSFQNVKQEL PQAILHHRPVRIRRSRIFLENRLEAGFLDFHFSSNIPGSNFSSWRFSSAHKVYRGLVLPIGTLTPSLSGT AIYYTRMLSPNAAHCQLSGSLSFDYRVALQKEYRHARHIVEPFCSFLKTTRPVLSSDEPHIFSIKDAFHS INLLHVGLESKVLNKHSTPSHLKLWTTYIFDEPHAKDTFPKTACWFSLPLTLQNTLSLDAEWIWKKSRWD HLNVIWEWILNDNLGLTLEFLHRSKYGFIKCAKDNYTLDVSRSLDTLLASPLSDRRNLITGKLFVRPHPH WNYNLNLRYGWHRPDSPSYLEYQMILGHKIFEHWQLFSVYEKREADKRCFFYLKLDKRKQKHRHPFG >gi|3328766|gb|AE001308.1:6085-8178[SEQ ID NO:40] GTGAAACGATTTTTCCCACTTTTTATTGGAGTGCTGCTCGCGCACACTTTGCCGTCAGAAGGTCTTTCTC ATCAACAAGCTGTCCAAAAAAAAATTTCTTATCTGAGCCATTTTAAAGGCATTACAGGAATTATGGATGT TGAGGATGGGGTATTACATATCCATGATGATCTACGTCTTCAAGCCAATAAAGCCTATGTTGAAAATCGC ACGGATTGTGGGATCAAAATCGTTGCTCATGGCAACGTTATGGTCAATTATCGCGGGAAAATTTTAATCT GTGATTATCTTGAGTACTATGAAGATACAGATTCTTGTTTACTCACCAATGGCCGCTGTTCGTTATACCC ATGGTTCATTGGAGGATCCACTATAACGATCTCACCATCTTCTATTATCATTCATAAAGGGTATATCTCG ACTTCTGAAGGTCCTCAGAAACATATTTGTTTATCCGGAGATTATTTAAAATACTCTTCAGACAGCGTAT TATCTATGGGACCCTCACGTCTTTCTATCTGTAATACGCCTGTGTTATTGCTTCCTCAAATCTCCATTAT GCCTATGGAGATTCCTAAGCCTCCGATTACTTTTCGAGGTGGGAGTGGAGGATTTCTGGGATCCTACTTA GGTGTTAGTTATTCCCCTATATCTAAAAAGCATTGTTCTACGACTTTGTTCTTGGATGGTTTTTTTAAAC ATGGAATAGGTCTCGGCTATAACATGCGCTTTTCCTCTCAGGAAAATCCAAGCAATGCCATAAATATTAA AAGCTATTACGCACATCGATTAGCTATTGATTCATCAGGAGCAAAAGATCGCTATCGATTACATGGAGAC TTCACTTTTTCCAAAGAACGAGCCCATCTTGCTGGTGAATTCCATTTAAGTGATAGCTGGGAAACAGTTG TGGATATCTTCCCAAATAACTTCTCTTTAAAAAATACAGGCCCTACAGAAGTTAGCCTATCATGGCGCGA TAACAATTTATTTGGGAAAATGACTTCCTCTGTCAAAGTCAACTCCTTTCAAAATGTTAAACAAGAATTG CCTCAAGCAATTCTTCATCACCGACCAGTACGTATCAGGCGCTCTCGCATTTTCCTAGAGAATCGCTTAG AAGCTGGTTTTTTAGATTTTCATTTCAGTAGTAATATTCCAGGCTCTAACTTCTCATCATGGAGGTTCTC ATCCGCTCACAAAGTCTACCGTGGGCTTGTTCTTCCTATAGGAACGTTAACCCCTTCGCTATCTGGAACT GCTATCTACTATACCCGCATGCTCTCCCCAAATGCAGCCCATTGTCAATTATCTGGATCGCTATCTTTTG ATTATCGCGTTGCTTTACAAAAAGAATATCGGCATGCAAGACATATTGTAGAGCCTTTTTGCTCCTTTTT AAAAACCACTCGTCCTGTATTATCCTCTGATGAGCCTCATATTTTCTCGATTAAAGATGCTTTTCACTCT ATCAACCTTCTACATGTAGGATTGGAGTCAAAAGTCTTAAACAAACATTCCACTCCTTCGCATCTGAAAC TATGGACGACCTATATCTTTGATGAACCTCACGCTAAGGACACTTTCCCTAAAACTGCTTGCTGGTTCTC TCTTCCTCTTACACTCCAAAATACTTTATCCTTAGATGCGGAATGGATTTGGAAAAAAAGCCGATGGGAT CATCTCAATGTAATCTGGGAATGGATTTTGAATGATAATCTCGGTCTTACTTTAGAATTTTTACATAGAA GTAAGTATGGCTTTATTAAGTGCGCTAAAGATAACTACACACTCGATGTAAGCCGATCTTTAGACACATT ACTAGCCTCTCCTCTTTCCGATCGAAGAAATTTGATTACTGGCAAACTTTTTGTTCGTCCACATCCTCAT TGGAATTATAATCTTAATCTTCGTTATGGATGGCATCGTCCAGACTCTCCATCCTATTTAGAATACCAGA TGATTCTGGGTCATAAAATCTTTGAGCACTGGCAGCTATTCTCTGTCTACGAAAAACGTGAAGCTGATAA GCGCTGCTTCTTTTATCTAAAATTAGATAAACGAAAACAGAAACACCGCCATCCTTTTGGATAA >gi|3329347|gb|AAC68470.| Putative Outer Membrane Protein H [Chlamydia trachomatis][SEQ ID NO:41] MPFSLRSTSFCFLACLCSYSYGFASSPQVLTPNVTTPFKGDDVYLNGDCAFVNVYAGAENGSIISANGDN LTITGQNHTLSFTDSQGPVLQNYAFISAGETLTLKDFSSLMFSKNVSCGEKGMISGKTVSISGAGEVIFW DNSVGYSPLSIVPASTPTPPAPAPAPAASSSLSPTVSDARKGSIFSVETSLEISGVKKGVMFDNNAGNFG TVFRGNSNNNAGSGGSGSATTPSFTVKNCKGKVSFTDNVASCGGGVVYKGTVLFKDNEGGIFFRGNTAYD DLGILAATSRDQNTETGGGGGVICSPDDSVKFEGNKGSIVFDYNFAKGRGGSILTKEFSLVADDSVVFSN NTAEKGGGAIYAPTIDISTNGGSILFERNRAAEGGAICVSEASSGSTGNLTLSASDGDIVFSGNMTSDRP GERSAARILSDGTTVSLNASGLSKLIFYDPVVQNNSAAGASTPSPSSSSMPGAVTINQSGNGSVIFTAES LTPSEKLQVLNSTSNFPGALTVSGGELVVTEGATLTTGTITATSGRVTLGSGASLSAVAGAANNNYTCTV SKLGIDLESFLTPNYKTAILGADGTVTVNSGSTLDLVMESEAEVYDNPLFVGSLTIPFVTLSSSSASNGV TKNSVTINDADAAHYGYQGSWSADWTKPPLAPDAKGMVPPNTNNTLYLTWRPASNYGEYRLDPQRKGELV PNSLWVAGSALRTFTNGLKEHYVSRDVGFVASLHALGDYILNYTQDDRDGFLARYGGFQATAASHYENGS IFGVAFGQLYGQTKSRMYYSKDAGNMTMLSCFGRSYVDIKGTETVMYWETAYGYSVHRMHTQYFNDKTQK FDHSKCHWHNNNYYAFVGAEHNFLEYCIPTRQFARDYELTGFMRFEMAGGWSSSTRETGSLTRYFARGSG HNMSLPIGIVAHAVSHVRRSPPSKLTLNMGYRPDIWRVTPHCNMEIIANGVKTPIQGSPLARHAFFLEVH DTLYIHHFGRAYMNYSLDARRRQTAHFVSMGLNRIF >gi|3329342|gb|AE001360.1:10808-13858[SEQ ID NO:42] ATGCCTTTTTCTTTGAGATCTACATCATTTTGTTTTTTAGCTTGTTTGTGTTCCTATTCGTATGGATTCG CGAGCTCTCCTCAAGTGTTAACACCTAATGTAACCACTCCTTTTAAGGGGGACGATGTTTACTTGAATGG AGACTGCGCTTTTGTCAATGTCTATGCAGGGGCAGAGAACGGCTCAATTATCTCAGCTAATGGCGACAAT TTAACGATTACCGGACAAAACCATACATTATCATTTACAGATTCTCAAGGGCCAGTTCTTCAAAATTATG CCTTCATTTCAGCAGGAGAGACACTTACTCTGAAAGATTTTTCGAGTTTGATGTTCTCGAAAAATGTTTC TTGCGGAGAAAAGGGAATGATCTCAGGGAAAACCGTGAGTATTTCCGGAGCAGGCGAAGTGATTTTTTGG GATAACTCTGTGGGGTATTCTCCTTTGTCTATTGTGCCAGCATCGACTCCAACTCCTCCAGCACCAGCAC CAGCTCCTGCTGCTTCAAGCTCTTTATCTCCAACAGTTAGTGATGCTCGGAAAGGGTCTATTTTTTCTGT AGAGACTAGTTTGGAGATCTCAGGCGTCAAAAAAGGGGTCATGTTCGATAATAATGCCGGGAATTTTGGA ACAGTTTTTCGAGGTAATAGTAATAATAATGCTGGTAGTGGGGGTAGTGGGTCTGCTACAACACCAAGTT TTACAGTTAAAAACTGTAAAGGGAAAGTTTCTTTCACAGATAACGTAGCCTCCTGTGGAGGCGGAGTAGT CTACAAAGGAACTGTGCTTTTCAAAGACAATGAAGGAGGCATATTCTTCCGAGGGAACACAGCATACGAT GATTTAGGGATTCTTGCTGCTACTAGTCGGGATCAGAATACGGAGACAGGAGGCGGTGGAGGAGTTATTT GCTCTCCAGATGATTCTGTAAAGTTTGAAGGCAATAAAGGTTCTATTGTTTTTGATTACAACTTTGCAAA AGGCAGAGGCGGAAGCATCCTAACGAAAGAATTCTCTCTTGTAGCAGATGATTCGGTTGTCTTTAGTAAC AATACAGCAGAAAAAGGCGGTGGAGCTATTTATGCTCCTACTATCGATATAAGCACGAATGGAGGATCGA TTCTATTTGAAAGAAACCGAGCTGCAGAAGGAGGCGCCATCTGCGTGAGTGAAGCAAGCTCTGGTTCAAC TGGAAATCTTACTTTAAGCGCTTCTGATGGGGATATTGTTTTTTCTGGGAATATGACGAGTGATCGTCCT GGAGAGCGCAGCGCAGCAAGAATCTTAAGTGATGGAACGACTGTTTCTTTAAATGCTTCCGGACTATCGA AGCTGATCTTTTATGATCCTGTAGTACAAAATAATTCAGCAGCGGGTGCATCGACACCATCACCATCTTC TTCTTCTATGCCTGGTGCTGTCACGATTAATCAGTCCGGTAATGGATCTGTGATTTTTACCGCCGAGTCA TTGACTCCTTCAGAAAAACTTCAAGTTCTTAACTCTACTTCTAACTTCCCAGGAGCTCTGACTGTGTCAG GAGGGGAGTTGGTTGTGACGGAAGGAGCTACCTTAACTACTGGGACCATTACAGCCACCTCTGGACGAGT GACTTTAGGATCCGGAGCTTCGTTGTCTGCCGTTGCAGGTGCTGCAAATAATAATTATACTTGTACAGTA TCTAAGTTGGGGATTGATTTAGAATCCTTTTTAACTCCTAACTATAAGACGGCCATACTGGGTGCGGATG GAACAGTTACTGTTAACAGCGGCTCTACTTTAGACCTAGTGATGGAGAGTGAGGCAGAGGTATATGATAA TCCGCTTTTTGTGGGATCGCTGACAATTCCTTTTGTTACTCTATCTTCTAGTAGTGCTAGTAACGGAGTT ACAAAAAATTCTGTCACTATTAATGATGCAGACGCTGCGCACTATGGGTATCAAGGCTCTTGGTCTGCAG ATTGGACGAAACCGCCTCTGGCTCCTGATGCTAAGGGGATGGTACCTCCTAATACCAATAACACTCTGTA TCTGACATGGAGACCTGCTTCGAATTACGGTGAATATCGACTGGATCCTCAGAGAAAGGGAGAACTAGTA CCCAACTCTCTTTGGGTAGCGGGATCTGCATTAAGAACCTTTACTAATGGTTTGAAAGAACACTATGTTT CTAGAGATGTTGGATTTGTAGCATCTCTGCATGCTCTCGGGGATTATATTTTGAATTATACGCAAGATGA TCGGGATGGCTTTTTAGCTAGATATGGGGGATTCCAGGCGACCGCAGCCTCCCATTATGAAAATGGGTCA ATATTTGGAGTGGCTTTTGGACAACTCTATGGTCAGACAAAGAGCAGAATGTATTACTCTAAAGATGCTG GGAACATGACGATGTTGTCCTGTTTCGGAAGAAGTTACGTAGATATTAAAGGAACAGAAACTGTTATGTA TTGGGAGACGGCTTATGGCTATTCTGTGCACAGAATGCATACGCAGTATTTTAATGACAAAACGCAGAAG TTCGATCATTCGAAATGTCATTGGCACAACAATAACTATTATGCGTTTGTGGGTGCCGAGCATAATTTCT TAGAGTACTGCATTCCTACTCGTCAGTTCGCTAGAGATTATGAGCTTACAGGGTTTATGCGTTTTGAAAT GGCCGGAGGATGGTCCAGTTCTACACGAGAAACTGGCTCCCTAACTAGATATTTCGCTCGCGGGTCAGGG CATAATATGTCGCTTCCAATAGGAATTGTAGCTCATGCAGTTTCTCATGTGCGAAGATCTCCTCCTTCTA AACTGACACTAAATATGGGATATAGACCAGACATTTGGCGTGTCACTCCACATTGCAATATGGAAATTAT TGCTAACGGAGTGAAGACACCTATACAAGGATCTCCGCTGGCACGGCATGCCTTCTTCTTAGAAGTGCAT GATACTTTGTATATTCATCATTTTGGAAGAGCCTATATGAACTATTCGCTGGATGCTCGTCGTCGACAAA CGGCACATTTTGTATCCATGGGCTTGAATAGAATCTTTTAA >gi|3328874|gb|AAC68042.1|60kDa Cysteine-Rich OMP [Chlamydia trachomatis][SEQ ID NO:43] MRIGDPMNKLIRRAVTIFAVTSVASLFASGVLETSMAESLSTNVISLADTKAKDNTSHKSKKARKNHSKE TPVDRKEVAPVHESKATGPKQDSCFGRMYTVKVNDDRNVEITQAVPEYATVGSPYPIEITATGKRDCVDV IITQQLPCEAEFVRSDPATTPTADGKLVWKIDRLGQGEKSKITVWVKPLKEGCCFTAATVCACPEIRSVT KCGQPAICVKQEGPENACLRCPVVYKINIVNQGTATARNVVVENPVPDGYAHSSGQRVLTFTLGDMQPGE HRTITVEFCPLKRGRATNIATVSYCGGHKNTASVTTVINEPCVQVSIAGADWSYVCKPVEYVISVSNPGD LVLRDVVVEDTLSPGVTVLEAAGAQISCNKVVWTVKELNPGESLQYKVLVRAQTPGQFTNNVVVKSCSDC GTCTSCAEATTYWKGVAATHMCVVDTCDPVCVGENTVYRICVTNRGSAEDTNVSLMLKFSKELQPVSFSG PTKGTITGNTVVFDSLPRLGSKETVEFSVTLKAVSAGDARGEAILSSDTLTVPVSDTENTHIY >gi|3328863|gb|AE001317.1:c11039-9378[SEQ ID NO:44] ATGCGAATAGGAGATCCTATGAACAAACTCATCAGACGAGCAGTGACGATCTTCGCGGTGACTAGTGTGG CGAGTTTATTTGCTAGCGGGGTGTTAGAGACCTCTATGGCAGAGTCTCTCTCTACAAACGTTATTAGCTT AGCTGACACCAAAGCGAAAGACAACACTTCTCATAAAAGCAAAAAAGCAAGAAAAAACCACAGCAAAGAG ACTCCCGTAGACCGTAAAGAGGTTGCTCCGGTTCATGAGTCTAAAGCTACAGGACCTAAACAGGATTCTT GCTTTGGCAGAATGTATACAGTCAAAGTTAATGATGATCGCAATGTTGAAATCACACAAGCTGTTCCTGA ATATGCTACGGTAGGATCTCCCTATCCTATTGAAATTACTGCTACAGGTAAAAGGGATTGTGTTGATGTT ATCATTACTCAGCAATTACCATGTGAAGCAGAGTTCGTACGCAGTGATCCAGCGACAACTCCTACTGCTG ATGGTAAGCTAGTTTGGAAAATTGACCGCTTAGGACAAGGCGAAAAGAGTAAAATTACTGTATGGGTAAA ACCTCTTAAAGAAGGTTGCTGCTTTACAGCTGCAACAGTATGCGCTTGTCCAGAGATCCGTTCGGTTACA AAATGTGGACAACCTGCTATCTGTGTTAAACAAGAAGGCCCAGAGAATGCTTGTTTGCGTTGCCCAGTAG TTTACAAAATTAATATAGTGAACCAAGGAACAGCAACAGCTCGTAACGTTGTTGTTGAAAATCCTGTTCC AGATGGTTACGCTCATTCTTCTGGACAGCGTGTACTGACGTTTACTCTTGGAGATATGCAACCTGGAGAG CACAGAACAATTACTGTAGAGTTTTGTCCGCTTAAACGTGGTCGTGCTACCAATATAGCAACGGTTTCTT ACTGTGGAGGACATAAAAATACAGCAAGCGTAACAACTGTGATCAACGAGCCTTGCGTACAAGTAAGTAT TGCAGGAGCAGATTGGTCTTATGTTTGTAAGCCTGTAGAATATGTGATCTCCGTTTCCAATCCTGGAGAT CTTGTGTTGCGAGATGTCGTCGTTGAAGACACTCTTTCTCCCGGAGTCACAGTTCTTGAAGCTGCAGGAG CTCAAATTTCTTGTAATAAAGTAGTTTGGACTGTGAAAGAACTGAATCCTGGAGAGTCTCTACAGTATAA AGTTCTAGTAAGAGCACAAACTCCTGGACAATTCACAAATAATGTTGTTGTGAAGAGCTGCTCTGACTGT GGTACTTGTACTTCTTGCGCAGAAGCGACAACTTACTGGAAAGGAGTTGCTGCTACTCATATGTGCGTAG TAGATACTTGTGACCCTGTTTGTGTAGGAGAAAATACTGTTTACCGTATTTGTGTCACCAACAGAGGTTC TGCAGAAGATACAAATGTTTCTTTAATGCTTAAATTCTCTAAAGAACTGCAACCTGTATCCTTCTCTGGA CCAACTAAAGGAACGATTACAGGCAATACAGTAGTATTCGATTCGTTACCTAGATTAGGTTCTAAAGAAA CTGTAGAGTTTTCTGTAACATTGAAAGCAGTATCAGCTGGAGATGCTCGTGGGGAAGCGATTCTTTCTTC CGATACATTGACTGTTCCAGTTTCTGATACAGAGAATACACACATCTATTAA >gi|3328841|gb|AAC68010.1| Putative outer membrane protein B [Chlamydia trachomatis][SEQ ID NO:45] MKWLSATAVFAAVLPSVSGFCFPEPKELNFSRVGTSSSTTFTETVGEAGAEYIVSGNASFTKFTNIPTTD TTTPTNSNSSSSNGETASVSEDSDSTTTTPDPKGGGAFYNAHSGVLSFMTRSGTEGSLTLSEIKITGEGG AIFSQGELLFTDLTGLTIQNNLSQLSGGAIFGESTISLSGITKATFSSNSAEVPAPVKKPTEPKAQTASE TSGSSSSSGNDSVSSPSSSRAEPAAANLQSHFICATATPAAQTDTETSTPSHKPGSGGAIYAKGDLTIAD SQEVLFSINKATKDGGAIFAEKDVSFENITSLKVQTNGAEEKGGAIYAKGDLSIQSSKQSLFNSNYSKQG GGALYVEGDINFQDLEEIRIKYNKAGTFETKKITLPKAQASAGNADAWASSSPQSGSGATTVSNSGDSSS GSDSDTSETVPATAKGGGLYTDKNLSITNITGIIEIANNKATDVGGGAYVKGTLTCENSHRLQFLKNSSD KQGGGIYGEDNITLSNLTGKTLFQENTAKEEGGGLFIKGTDKALTMTGLDSFCLINNTSEKHGGGAFVTK EISQTYTSDVETIPGITPVHGETVITGNKSTGGNGGGVCTKRLALSNLQSISISGNSAAENGGGAHTCPD SFPTADTAEQPAAASAATSTPESAPVVSTALSTPSSSTVSSLTLLAASSQASPATSNKETQDPNADTDLL IDYVVDTTISKNTAKKGGGIYAKKAKMSRIDQLNISENSATEIGGGICCKESLELDALVSLSVTENLVGK EGGGLHAKTVNISNLKSGFSFSNNKANSSSTGVATTASAPAAAAASLQAAAAAVPSSPATPTYSGVVGGA IYGEKVTFSQCSGTCQFSGNQAIDNNPSQSSLNVQGGAIYAKTSLSIGSSDAGTSYIFSGNSVSTGKSQT TGQIAGGAIYSPTVTLNCPATFSNNTASMATPKTSSEDGSSGNSIKDTIGGAIAGTAITLSGVSRFSGNT ADLGAAIGTLANANTPSATSGSQNSITEKITLENGSFIFERNQANKRGAIYSPSVSIKGNNITFNQNTST HDGSAIYFTKDATIESLGSVLFTGNNVTATQASSATSGQNTNTANYGAAIFGDPGTTQSSQTDAILTLLA SSGNITFSNNSLQNNQGDTPASKFCSIAGYVKLSLQAAKGKTISFFDCVHTSTKKIGSTQNVYETLDINK EENSNPYTGTIVFSSELHENKSYIPQNAILHNGTLVLKEKTELHVVSFEQKEGSKLIMKPGAVLSNQNIA NGALVINGLTIDLSSMGTPQAGEIFSPPELRIVATTSSASGGSGVSSSIPTNPKRISAAAPSGSAATTPT MSENKVFLTGDLTLIDPNGNFYQNPMLGSDLDVPLIKLPTNTSDVQVYDLTLSGDLFPQKGYMGTWTLDS NPQTGKLQARWTFDTYRRWVYIPRDNHFYANSILGSQNSMIVVKQGLINNMLNNARFDDIAYNNFWVSGV GTFLAQQGTPLSEEFSYYSRGTSVAIDAKPRQDFILGAAFSKMVGKTKAIKKMHNYFHKGSEYSYQASVY GGKFLYFLLNKQHGWALPFLIQGVVSYGHIKHDTTTLYPSIHERNKGDWEDLGWLADLRISMDLKEPSKD SSKRITVYGELEYSSIRQKQFTEIDYDPRHFDDCAYRNLSLPVGCAVEGAIMNCNILMYNKLALAYMPSI YRNNPVCKYRVLSSNEAGQVICGVPTRTSARAEYSTQLYLGPFWTLYGNYTIDVGMYTLSQMTSCGARMI F >gi|3328833|gb|AE001314.1:9601-14856[SEQ ID NO:46] ATGAAATGGCTGTCAGCTACTGCGGTGTTTGCTGCTGTTCTCCCCTCAGTTTCAGGGTTTTGCTTCCCAG AACCTAAAGAATTAAATTTCTCTCGCGTAGGAACTTCTTCCTCTACCACTTTTACTGAAACAGTTGGAGA AGCTGGGGCAGAATATATCGTCTCTGGTAACGCATCTTTCACAAAATTTACCAACATTCCTACTACCGAT ACAACAACTCCCACGAACTCAAACTCCTCTAGCTCTAACGGAGAGACTGCTTCCGTTTCTGAGGATAGTG ACTCTACAACAACGACTCCTGATCCTAAAGGTGGCGGCGCCTTTTATAACGCGCACTCCGGAGTTTTATC CTTTATGACACGATCAGGAACAGAAGGTTCCTTAACTCTGTCTGAGATAAAAATAACTGGTGAAGGCGGT GCTATCTTCTCTCAAGGAGAGCTGCTATTTACAGATCTGACAGGTCTAACCATCCAAAATAACTTATCCC AGCTATCCGGAGGAGCGATTTTTGGAGAATCTACAATCTCCCTATCAGGGATTACTAAAGCGACTTTCTC CTCCAACTCTGCAGAAGTTCCTGCTCCTGTTAAGAAACCTACAGAACCTAAAGCTCAAACAGCAAGCGAA ACGTCGGGTTCTAGTAGTTCTAGCGGAAATGATTCGGTGTCTTCCCCCAGTTCCAGTAGAGCTGAACCCG CAGCAGCTAATCTTCAAAGTCACTTTATTTGTGCTACAGCTACTCCTGCTGCTCAAACCGATACAGAAAC ATCAACTCCCTCTCATAAGCCAGGATCTGGGGGAGCTATCTATGCTAAAGGCGACCTTACTATCGCAGAC TCTCAAGAGGTACTATTCTCAATAAATAAAGCTACTAAAGATGGAGGAGCGATCTTTGCTGAGAAAGATG TTTCTTTCGAGAATATTACATCATTAAAAGTACAAACTAACGGTGCTGAAGAAAAGGGAGGAGCTATCTA TGCTAAAGGTGACCTCTCAATTCAATCTTCTAAACAGAGTCTTTTTAATTCTAACTACAGTAAACAAGGT GGTGGGGCTCTATATGTTGAAGGAGATATAAACTTCCAAGATCTTGAAGAAATTCGCATTAAGTACAATA AAGCTGGAACGTTCGAAACAAAAAAAATCACTTTACCAAAAGCTCAAGCATCTGCAGGAAATGCAGATGC TTGGGCCTCTTCCTCTCCTCAATCTGGTTCTGGAGCAACTACAGTCTCCAACTCAGGAGACTCTAGCTCT GGCTCAGACTCGGATACCTCAGAAACAGTTCCAGCCACAGCTAAAGGCGGTGGGCTTTATACTGATAAGA ATCTTTCGATTACTAACATCACAGGAATTATCGAAATTGCAAATAACAAAGCGACAGATGTTGGAGGTGG TGCTTACGTAAAAGGAACCCTTACTTGTGAAAACTCTCACCGTCTACAATTTTTGAAAAACTCTTCCGAT AAACAAGGTGGAGGAATCTACGGAGAAGACAACATCACCCTATCTAATTTGACAGGGAAGACTCTATTCC AAGAGAATACTGCCAAAGAAGAGGGCGGTGGACTCTTCATAAAAGGTACAGATAAAGCTCTTACAATGAC AGGACTGGATAGTTTCTGTTTAATTAATAACACATCAGAAAAACATGGTGGTGGAGCCTTTGTTACCAAA GAAATCTCTCAGACTTACACCTCTGATGTGGAAACAATTCCAGGAATCACGCCTGTACATGGTGAAACAG TCATTACTGGCAATAAATCTACAGGAGGTAATGGTGGAGGCGTGTGTACAAAACGTCTTGCCTTATCTAA CCTTCAAAGCATTTCTATATCCGGGAATTCTGCAGCTGAAAATGGTGGTGGAGCCCACACATGCCCAGAT AGCTTCCCAACGGCGGATACTGCAGAACAGCCCGCAGCAGCTTCTGCCGCGACGTCTACTCCCGAGTCTG CCCCAGTGGTCTCAACTGCTCTAAGCACACCTTCATCTTCTACCGTCTCTTCATTAACCTTACTACCAGC CTCTTCACAAGCCTCTCCTGCAACCTCTAATAAGGAAACTCAAGATCCTAATGCTGATACAGACTTATTG ATCGATTATGTAGTTGATACGACTATCAGCAAAAACACTGCTAAGAAAGGCGGTGGAATCTATGCTAAAA AAGCCAAGATGTCCCGCATAGACCAACTGAATATCTCTGAGAACTCCGCTACAGAGATAGGTGGAGGTAT CTGCTGTAAAGAATCTTTAGAACTAGATGCCCTAGTCTCCTTATCTGTAACAGAGAACCTTGTTGGGAAA GAAGGTGGAGGCTTACATGCTAAAACTGTAAATATTTCTAATCTGAAATCAGGCTTCTCTTTCTCGAACA ACAAAGCAAACTCCTCATCCACAGGAGTCGCAACAACAGCTTCAGCACCTGCTGCAGCTGCTGCTTCCCT ACAAGCAGCCGCAGCAGCCGTACCATCATCTCCAGCAACACCAACTTATTCAGGTGTAGTAGGAGGAGCT ATCTATGGAGAAAAGGTTACATTCTCTCAATGTAGCGGGACTTGTCAGTTCTCTGGGAACCAAGCTATCG ATAACAATCCCTCCCAATCATCGTTGAACGTACAAGGAGGAGCCATCTATGCCAAAACCTCTTTGTCTAT TGGATCTTCCGATGCTGGAACCTCCTATATTTTCTCGGGGAACAGTGTCTCCACTGGGAAATCTCAAACA ACAGGGCAAATAGCGGGAGGAGCGATCTACTCCCCTACTGTTACATTGAATTGTCCTGCGACATTCTCTA ACAATACAGCCTCTATGGCTACACCAAAGACTTCTTCTGAAGATGGATCCTCAGGAAATTCTATTAAAGA TACCATTGGAGGAGCCATTGCAGGGACAGCCATTACCCTATCTGGAGTCTCTCGATTTTCAGGGAATACG GCTGATTTAGGAGCTGCAATAGGAACTCTAGCTAATGCAAATACACCCAGTGCAACTAGCGGATCTCAAA ATAGCATTACAGAAAAAATTACTTTAGAAAACGGTTCTTTTATTTTTGAAAGAAACCAAGCTAATAAACG TGGAGCGATTTACTCTCCTAGCGTTTCCATTAAAGGGAATAATATTACCTTCAATCAAAATACATCCACT CATGATGGAAGTGCTATCTACTTTACAAAAGATGCTACGATTGAGTCTTTAGGATCTGTTCTTTTTACAG GAAATAACGTTACAGCTACACAAGCTAGTTCTGCAACATCTGGACAAAATACAAATACTGCCAACTATGG GGCAGCCATCTTTGGAGATCCAGGAACCACTCAATCGTCTCAAACAGATGCCATTTTAACCCTTCTTGCT TCTTCTGGAAACATTACTTTTAGCAACAACAGTTTACAGAATAACCAAGGTGATACTCCCGCTAGCAAGT TTTGTAGTATTGCAGGATACGTCAAACTCTCTCTACAAGCCGCTAAAGGGAAGACTATTAGCTTTTTCGA TTGTGTGCACACCTCTACCAAAAAAATAGGTTCAACACAAAACGTTTATGAAACTTTAGATATTAATAAA GAAGAGAACAGTAATCCATATACAGGAACTATTGTGTTCTCTTCTGAATTACATGAAAACAAATCTTACA TCCCACAGAATGCAATCCTTCACAACGGAACTTTAGTTCTTAAAGAGAAAACAGAACTCCACGTAGTCTC TTTTGAGCAGAAAGAAGGGTCTAAATTAATTATGAAACCCGGAGCTGTGTTATCTAACCAAAACATAGCT AACGGAGCTCTAGTTATCAATGGGTTAACGATTGATCTTTCCAGTATGGGGACTCCTCAAGCAGGGGAAA TCTTCTCTCCTCCAGAATTACGTATCGTTGCCACGACCTCTAGTGCATCCGGAGGAAGCGGGGTCAGCAG TAGTATACCAACAAATCCTAAAAGGATTTCTGCAGCAGCGCCTTCAGGTTCTGCCGCAACTACTCCAACT ATGAGCGAGAACAAAGTTTTCCTAACAGGAGACCTTACTTTAATAGATCCTAATGGAAACTTTTACCAAA ACCCTATGTTAGGAAGCGATCTAGATGTACCACTAATTAAGCTTCCGACTAACACAAGTGACGTCCAAGT CTATGATTTAACTTTATCTGGGGATCTTTTCCCTCAGAAAGGGTACATGGGAACCTGGACATTAGATTCT AATCCACAAACAGGGAAACTTCAAGCCAGATGGACATTCGATACCTATCGTCGCTGGGTATACATACCTA GGGATAAATCATTTTTATGCGAACTCTATCTTAGGCTCCCAAACTCAATGATTGTTGTGAAGCAAGGGCT TATCAACAACATGTTGAATAATGCCCGCTTCGATGATATCGCTTACAATAACTTCTGGGTTTCAGGAGTA GGAACTTTCTTAGCTCAACAAGGAACTCCTCTTTCCGAAGAATTCAGTTACTACAGCCGCGGAACTTCAG TTGCCATCGATGCCAAACCTAGACAAGATTTTATCCTAGGAGCTGCATTTAGTAAGATGGTGGGGAAAAC CAAAGCCATCAAAAAAATGCATAATTACTTCCATAAGGGCTCTGAGTACTCTTACCAAGCTTCTGTCTAT GGAGGTAAATTCCTGTATTTCTTGCTCAATAAGCAACATGGTTGGGCACTTCCTTTCCTAATACAAGGAG TCGTGTCCTATGGACATATTAAACATGATACAACAACACTTTACCCTTCTATCCATGAAAGAAATAAAGG AGATTGGGAAGATTTAGGATGGTTAGCGGATCTTCGTATCTCTATGGATCTTAAAGAACCTTCTAAAGAT TCTTCTAAACGGATCACTGTCTATGGGGAACTTGAGTATTCCAGCATTCGCCAGAAACAGTTCACAGAAA TCGATTACGATCCAAGACACTTCGATGATTGTGCTTACAGAAATCTGTCGCTTCCTGTGGGATGCGCTGT CGAAGGAGCTATCATGAACTGTAATATTCTTATGTATAATAAGCTTGCATTAGCCTACATGCCTTCTATC TACAGAAATAATCCTGTCTGTAAATATCGGGTATTGTCTTCGAATGAAGCTGGTCAAGTTATCTGCGGAG TGCCAACTAGAACCTCTGCTAGAGCAGAATACAGTACTCAACTATATCTTGGTCCCTTCTGGACTCTCTA CGGAAACTATACTATCGATGTAGGCATGTATACGCTATCGCAAATGACTAGCTGCGGTGCTCGCATGATC TTCTAA >gi|3328840|gb|AAC68009.1| Putative outer membrane protein A [Chlamydia trachomatis][SEQ ID NO:47] MNRVIEIHAHYDQRQLSQSPNTNFLVHHPYLTLIPKFLLGALIVYAPYSFAEMELAISGHKQGKDRDTFT MISSCPEGTNYIINRKLILSDFSLLNKVSSGGAFRNLAGKISFLGKNSSASIHFKHININGFGAGVFSES SIEFTDLRKLVAFGSESTGGIFTAKEDISFKNNHHIAFRNNITKGNGGVIQLQGDMKGSVSFVDQRGAII FTNNQAVTSSSMKHSGRGGAISGDFAGSRILFLNNQQITFEGNSAVHGGAIYNKNGLVEFLGNAGPLAFK ENTTIANGGAIYTSNFKANQQTSPILFSQNHANKKGGAIYAQYVNLEQNQDTIRFEKNTAKEGGGAITSS QCSITAHNTIIFSDNAAGDLGGGAILLEGKKPSLTLIAHSGNIAFSGNTMLHITKKASLDRHNSILIKEA PYKIQLAANKNHSIHFFDPVMALSASSSPIQINAPEYETPFFSPKGMIVFSGANLLDDAREDVANRTSIF NQPVHLYNGTLSIENGAHLIVQSFKQTGGRISLSPGSSLALYTMNSFFHGNISSKEPLEINGLSFGVDIS PSNLQAEIRAGNAPLRLSGSPSIHDPEGLFYENRDTAASPYQMEILLTSDKIVDISKFTTDSLVTNKQSG FQGAWHFSWQPNTINNTKQKILRASWLPTGEYVLESNRVGRAVPNSLWSTFLLLQTASHNLGDHLCNNRS LIPTSYFGVLIGGTGAEMSTHSSEEESFISRLGATGTSIIRLTPSLTLSGGGSHMFGDSFVADLPEHITS EGIVQNVGLTHVWGPLTVNSTLCAALDHNAMVRICSKKDHTYGKWDTFGMRGTLGASYTFLEYDQTMRVF SFANIEATNILQRAFTETGYNPRSFSKTKLLNIAIPIGIGYEFCLGNSSFALLGKGSIGYSRDIKRENPS TLAHLAMNDFAWTTNGCSVPTSAHTLANQLILRYKACSLYITAYTINREGKNLSNSLSCGGYVGF >gi|3328833|gb|AE001314.1:6535-9462[SEQ ID NO:48] ATGAATCGAGTTATAGAAATCCATGCTCACTACGATCAAAGACAACTTTCTCAATCTCCAAATACAAACT TCTTAGTACATCATCCTTATCTTACTCTTATTCCCAAGTTTCTACTAGGAGCTCTAATCGTCTATGCTCC TTATTCGTTTGCAGAAATGGAATTAGCTATTTCTGGACATAAACAAGGTAAAGATCGAGATACCTTTACC ATGATCTCTTCCTGTCCTGAAGGCACTAATTACATCATCAATCGCAAACTCATACTCAGTGATTTCTCGT TACTAAATAAAGTTTCATCAGGGGGAGCCTTTCGGAATCTAGCAGGGAAAATTTCCTTCTTAGGAAAAAA TTCTTCTGCGTCCATTCATTTTAAACACATTAATATCAATGGTTTTGGAGCCGGAGTCTTTTCTGAATCC TCTATTGAATTTACTGATTTACGAAAACTTGTTGCTTTTGGATCTGAAAGCACAGGAGGAATTTTTACTG CGAAAGAGGACATCTCTTTTAAAAACAACCACCACATTGCCTTCCGCAATAATATCACCAAAGGGAATGG TGGCGTTATCCAGCTCCAAGGAGATATGAAAGGAAGCGTATCCTTTGTAGATCAACGTGGAGCTATCATC TTTACCAATAACCAAGCTGTAACTTCTTCATCAATGAAACATAGTGGTCGTGGAGGAGCAATTAGCGGTG ACTTCGCAGGATCCAGAATTCTTTTTCTTAATAACCAACAAATTACTTTCGAAGGCAATAGCGCTGTGCA TGGAGGTGCTATCTACAATAAGAATGGCCTTGTCGAGTTCTTAGGAAATGCAGGACCTCTTGCCTTTAAA GAGAACACAACAATAGCTAACGGGGGAGCTATATACACAAGTAATTTCAAAGCGAATCAACAAACATCCC CCATTCTATTCTCTCAAAATCATGCGAATAAGAAAGGCGGAGCGATTTACGCGCAATATGTGAACTTAGA ACAGAATCAAGATACTATTCGCTTTGAAAAAAATACCGCTAAAGAAGGCGGTGGAGCCATCACCTCTTCT CAATGCTCAATTACTGCTCATAATACCATCATTTTTTCCGATAATGCTGCCGGAGATCTTGGAGGAGGAG CAATTCTTCTAGAAGGGAAAAAACCTTCTCTAACCTTGATTGCTCATAGTGGTAATATTGCATTTAGCGG CAATACCATGCTTCATATCACCAAAAAAGCTTCCCTAGATCGACACAATTCTATCTTAATCAAAGAAGCT CCCTATAAAATCCAACTTGCAGCGAACAAAAACCATTCTATTCATTTCTTTGATCCTGTCATGGCATTGT CAGCATCATCTTCCCCTATACAAATCAATGCTCCTGAGTATGAAACTCCCTTCTTCTCACCTAAGGGTAT GATCGTTTTCTCGGGTGCGAATCTTTTAGATGATGCTAGGGAAGATGTTGCAAATAGAACATCGATTTTT AACCAACCCGTTCATCTATATAATGGCACCCTATCTATCGAAAATGGAGCCCATCTGATTGTCCAAAGCT TCAAACAGACCGGAGGACGTATCAGTTTATCTCCAGGATCCTCCTTGGCTCTATACACGATGAACTCGTT CTTCCATGGCAACATATCCAGCAAAGAACCCCTAGAAATTAATGGTTTAAGCTTTGGAGTAGATATCTCT CCTTCTAATCTTCAAGCAGAGATCCGTGCCGGCAACGCTCCTTTACGATTATCCGGATCCCCATCTATCC ATGATCCTGAAGGATTATTCTACGAAAATCGCGATACTGCAGCATCACCATACCAAATGGAAATCTTGCT CACCTCTGATAAAATTGTAGATATCTCCAAATTTACTACTGATTCTCTAGTTACGAACAAACAATCAGGA TTCCAAGGAGCCTGGCATTTTAGCTGGCAGCCAAATACTATAAACAATACTAAACAAAAAATATTAAGAG CTTCTTGGCTCCCAACAGGAGAATATGTCCTTGAATCCAATCGAGTGGGGCGTGCCGTTCCTAATTCCTT ATGGAGCACATTTTTACTTTTACAGACAGCCTCTCATAACTTAGGCGATCATCTATGTAATAATCGATCT CTTATTCCTACTTCATACTTCGGAGTTTTAATTGGAGGAACTGGAGCAGAAATGTCTACCCACTCCTCAG AAGAAGAAAGCTTTATATCTCGTTTAGGAGCTACAGGAACCTCTATCATACGCTTAACTCCCTCCCTGAC ACTCTCTGGAGGAGGCTCACATATGTTCGGAGATTCGTTCGTTGCAGACTTACCAGAACACATCACTTCA GAAGGAATTGTTCAGAATGTCGGTTTAACCCATGTCTGGGGACCCCTTACTGTCAATTCTACATTATGTG CAGCCTTAGATCACAACGCGATGGTCCGCATATGCTCCAAAAAAGATCACACCTATGGGAAATGGGATAC ATTCGGTATGCGAGGAACATTAGGAGCCTCTTATACATTCCTAGAATATGATCAAACTATGCGCGTATTC TCATTCGCCAACATCGAAGCCACAAATATCTTGCAAAGAGCTTTTACTGAAACAGGCTATAACCCAAGAA GTTTTTCCAAGACAAAACTTCTAAACATCGCCATCCCCATAGGGATTGGTTATGAATTCTGCTTAGGGAA TAGCTCTTTTGCTCTACTAGGTAAGGGATCCATCGGTTACTCTCGAGATATTAAACGAGAAAACCCATCC ACTCTTGCTCACCTGGCTATGAATGATTTTGCTTGGACTACCAATGGCTGTTCAGTTCCAACCTCTGCAC ACACATTGGCAAATCAATTGATTCTTCGCTATAAAGCATGTTCCTTATACATCACGGCATATACTATCAA CCGTGAAGGGAAGAACCTCTCCAATAGCTTATCCTGCGGAGGCTATGTTGGCTTCTAA >gi|3328763|gb|AAC67938.1| O-Sialoglycoprotein Endopeptidase family [Chlamydia trachomatis][SEQ ID NO:49] MYKYFIVDTSGSQPFLAYVDCRDVLEVWSLPTGPDQGVVLNFIFNSLDLPFQGIGVSVGPGGFSATRVGV AFAQGLSLAKNVPLVGYSSLEGYLSLGQEEEALLLPLGKKGGIVALNSELSLDGFLLTDTTSTPGILLSY SEALEYCLDKGCCHVISPDPTYFVELFSSRISVRKVVPCIDRIRKYVVSQFVLSQNLPLCLDYRSISSFF >gi|3328757|gb|AE001307.1:c6730-6098[SEQ ID NO:50] GTGTACAAATATTTTATTGTAGACACTTCCGGTTCTCAGCCGTTTTTGGCCTATGTCGATTGTCGAGATG TTTTAGAAGTATGGTCTTTGCCTACAGGGCCAGATCAAGGAGTTGTGTTAAATTTCATTTTCAACAGCCT GGATTTGCCTTTCCAAGGTATTGGAGTCTCTGTTGGTCCTGGGGGATTTTCTGCAACTAGAGTGGGAGTT GCTTTTGCTCAAGGGCTCTCTCTGGCTAAAAATGTCCCTTTAGTTGGCTATAGCTCTTTAGAAGGATATC TTTCTTTGGGTCAAGAAGAGGAGGCTTTGCTTTTGCCTCTAGGGAAAAAGGGTGGGATTGTAGCTTTAAA CTCAGAGCTTTCTCTTGATGGTTTTCTGCTTACAGATACTACTTCTACTCCGGGAATTTTATTGTCTTAT TCTGAAGCTCTAGAGTATTGTTTAGATAAGGGATGTTGTCATGTGATCTCTCCGGATCCAACGTATTTCG TAGAACTATTTTCTTCGCGTATTTCGGTAAGGAAGGTGGTTCCTTGTATCGATCGAATCCGTAAGTACGT TGTTTCGCAGTTTGTTCTGTCTCAAAATCTTCCGTTGTGTTTAGATTACCGGAGCATCTCTTCCTTTTTT TAG >gi|6578102|gb|AAC67897.2| ATP Synthase Subunit K [Chlamydia trachomatis][SEQ ID NO:51] MIDVSVVGPVLAMALAMIGSAVGCGMAGVASHAVMSRIDEGHGKIIGLSAMPSSQSIYGLIFMLLLNDAI KDGKVSAVSGIVMGIAVGSALLLSAFMQGKCCVSAIQAYARSSAIYGKSFASIGIVESFALFAFVFALLL F >gi|3328718|gb|AE001303.1:c956-531[SEQ ID NO:52] ATGATAGATGTATCAGTAGTGGGGCCTGTATTGGCTATGGCTTTGGCAATGATTGGTAGCGCTGTTGGAT GTGGAATGGCTGGAGTCGCTTCTCACGCAGTGATGTCTCGAATCGATGAAGGACACGGGAAGATTATTGG TCTGTCTGCTATGCCCTCATCACAATCCATTTACGGGTTGATTTTCATGTTACTGCTGAATGATGCAATT AAGGATGGAAAAGTCTCTGCAGTCAGTGGTATCGTAATGGGTATAGCTGTAGGATCTGCGTTATTGCTTT CTGCTTTTATGCAAGGGAAGTGCTGTGTGAGTGCTATTCAAGCCTATGCGCGTTCCTCTGCAATATATGG TAAATCATTTGCTTCGATTGGGATTGTTGAGTCTTTTGCGTTATTTGCTTTCGTTTTTGCGCTATTGTTA TTCTAA >gi|3329252|gb|AAC68382.1| S14 Ribosomal Protein [Chlamydia trachomatis][SEQ ID NO:53] MAKKSAVAREVKRRKLVEANFQKRAELRKLAKSLSVSEEERERAREALNKMRRDTSPSRLHNRCLLTGRP RGYLRKFAISRICFRQMASMGDIPGVVKASW >gi|3522908|gb|AE001351.1:2436-2741[SEQ ID NO:54] ATGGCGAAGAAGTCAGCAGTAGCTAGAGAAGTTAAACGTCGAAAGTTAGTAGAAGCTAATTTTCAGAAGA GAGCAGAGCTTCGAAAACTTGCGAAGAGTTTATCTGTTAGCGAGGAAGAAAGAGAAAGAGCTCGGGAAGC TCTCAATAAAATGAGAAGAGATACTTCTCCTTCTCGTTTACATAATAGATGCCTATTAACAGGCCGTCCT CGTGGATACCTTAGAAAGTTTGCTATCTCAAGAATTTGTTTTAGACAAATGGCTTCTATGGGAGATATCC CAGGCGTTGTGAAAGCAAGTTGGTAG >gi|3329133|gb|AAC68276.1| Major Outer Membrane Protein [Chlamydia trachomatis][SEQ ID NO:55] MKKLLKSVLVFAALSSASSLQALPVGNPAEPSLMIDGILWEGFGGDPCDPCATWCDAISMRVGYYGDFVF DRVLKTDVNKEFQMGAKPTTDTGNSAAPSTLTARENPAYGRHMQDAEMFTNAACMALNIWDRFDVFCTLG ATSGYLKGNSASFNLVGLFGDNENQKTVKAESVPNMSFDQSVVELYTDTTFAWSVGARAALWECGCATLG ASFQYAQSKPKVEELNVLCNAAEFTINKPKGYVGKEFPLDLTAGTDAATGTKDASIDYHEWQASLALSYR LNMFTPYIGVKWSRASFDADTIRIAQPKSATAIFDTTTLNPTIAGAGDVKTGAEGQLGDTMQIVSLQLNK MKSRKSCGIAVGTTIVDADKYAVTVETRLIDERAAHVNAQFRF >gi|3329126|gb|AE001338.1:c6759-5578[SEQ ID NO:56] ATGAAAAAACTCTTGAAATCGGTATTAGTATTTGCCGCTTTGAGTTCTGCTTCCTCCTTGCAAGCTCTGC CTGTGGGGAATCCTGCTGAACCAAGCCTTATGATCGACGGAATTCTGTGGGAAGGTTTCGGCGGAGATCC TTGCGATCCTTGCGCCACTTGGTGTGACGCTATCAGCATGCGTGTTGGTTACTACGGAGACTTTGTTTTC GACCGTGTTTTGAAAACTGATGTGAATAAAGAATTTCAGATGGGTGCCAAGCCTACAACTGATACAGGCA ATAGTGCAGCTCCATCCACTCTTACAGCAAGAGAGAATCCTGCTTACGGCCGACATATGCAGGATGCTGA GATGTTTACAAATGCCGCTTGCATGGCATTGAATATTTGGGATCGTTTTGATGTATTCTGTACATTAGGA GCCACCAGTGGATATCTTAAAGGAAACTCTGCTTCTTTCAATTTAGTTGGATTGTTTGGAGATAATGAAA ATCAAAAAACGGTCAAAGCGGAGTCTGTACCAAATATGAGCTTTGATCAATCTGTTGTTGAGTTGTATAC AGATACTACTTTTGCGTGGAGCGTCGGCGCTCGCGCAGCTTTGTGGGAATGTGGATGTGCAACTTTAGGA GCTTCATTCCAATATGCTCAATCTAAACCTAAAGTAGAAGAATTAAACGTTCTCTGCAATGCAGCAGAGT TTACTATTAATAAACCTAAAGGGTATGTAGGTAAGGAGTTTCCTCTTGATCTTACAGCAGGAACAGATGC TGCGACAGGAACTAAGGATGCCTCTATTGATTACCATGAATGGCAAGCAAGTTTAGCTCTCTCTTACAGA CTGAATATGTTCACTCCCTACATTGGAGTTAAATGGTCTCGAGCAAGCTTTGATGCCGATACGATTCGTA TAGCCCAGCCAAAATCAGCTACAGCTATTTTTGATACTACCACGCTTAACCCAACTATTGCTGGAGCTGG CGATGTGAAAACTGGCGCAGAGGGTCAGCTCGGAGACACAATGCAAATCGTTTCCTTGCAATTGAACAAG ATGAAATCTAGAAAATCTTGCGGTATTGCAGTAGGAACAACTATTGTGGATGCAGACAAATACGCAGTTA CAGTTGAGACTCGCTTGATCGATGAGAGAGCAGCTCACGTAAATGCACAATTCCGCTTCTAA >gi|3328987|gb|AAC68150.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO:57] MLKMFWLNSLVFFSLLLSACGYTVLSPHYVEKKFSLSEGIYVCPIEGDSLGDLVSSLSYELEKRGLHTRS QGTSSGYVLKVSLFNETDENIGFAYTPQKPDEKPVKHFIVSNEGRLALSAKVQLIKNRTQEILVEKCLRK SVTFDFQPDLGTANAHQLALGQFEMHNEAIKSASRILYSQLAETIVQQVYYDLF >gi|3328980|gb|AE001325.1:10880-11464[SEQ ID NO:58] ATGCTGAAAATGTTTTGGTTGAATAGCCTCGTTTTCTTCTCGTTACTACTATCAGCCTGCGGCTATACAG TGCTCTCCCCCCACTATGTAGAAAAGAAATTCTCGCTTTCCGAAGGCATCTATGTCTGCCCTATCGAAGG AGATTCATTAGGAGATCTCGTATCCTCTCTTTCTTACGAATTAGAAAAGCGAGGACTCCACACACGATCT CAAGGAACCTCTTCTGGTTATGTACTCAAAGTCTCTCTTTTCAATGAGACTGATGAAAATATTGGATTCG CATACACTCCCCAAAAACCTGATGAAAAACCTGTAAAACACTTCATTGTCTCTAATGAAGGGCGCTTAGC GTTATCAGCAAAAGTCCAACTAATCAAAAACCGCACACAAGAAATATTAGTGGAGAAATGCCTGAGAAAA TCGGTTACTTTTGATTTTCAACCTGACCTCGGAACCGCGAATGCTCATCAGCTAGCTCTCGGACAATTTG AAATGCATAATGAAGCAATAAAAAGCGCTTCTCGTATATTGTATTCGCAATTAGCAGAGACTATTGTACA ACAGGTATACTATGACCTTTTCTGA >gi|3328972|gb|AAC68136.1| Apolipoprotein N-Acetyltransferase [Chlamydia trachomatis][SEQ ID NO:59] MFKLVSYIILSWVLVCLAQPDVSVVASVVSCICGYSLLWAGLFALVEQLSWKKVWCIAFIWTWTVEGAHF SWMLEDLYVGTSIYFVWGILLSYLATLFASFSCLVVWCCRKQYRGALVWLPGVWVAIEAIRYYGLLSGVS FDFIGWPLTATAYGRQFGSFFGWAGQSFLVIAANICCFAVCLLKHSFSKGLWLTLCAFPYLLGGAHYEYL KKHFSDSEVLRVAIVQPGYSPHMHAGRTASAIWRGLVSLCQTIQTPVDVIVFPEVSVPFGLHRQAYTLHE NQPVLESLLPNKSWGEFFTNLDWIQAIAERYQCTVIMGMERWENKGGILHLYNAAECVSREGEITSYDKR ILVPGGEYIPGGKIGFSLCQTFFPEFALPFQRLPGEFSGVVNITERIKAGISICYEETFGYAIRPYKRQQ ADILVNLTNDGWYPRSRLPLVHFYHGMLRNQELGIPCIRACRTGVSAAVDSLGRIVGILPWESRTCPVST GVLQVSVPLYSYHTVYARLGDAPLLLIAVCSVIGAIAYFYRKKKETPPQTFF >gi|3328966|gb|AE001324.1:c6152-4524[SEQ ID NO:60] GTGTTTAAACTTGTGTCATACATCATCCTTTCTTGGGTGCTGGTCTGTTTGGCTCAGCCGGATGTAAGTG TTGTAGCTTCTGTTGTTAGTTGTATTTGCGGTTACAGCTTACTTTGGGCTGGGCTTTTTGCTTTAGTAGA GCAATTATCTTGGAAGAAAGTTTGGTGCATCGCTTTTATTTGGACTTGGACTGTCGAAGGCGCTCATTTC TCTTGGATGCTTGAAGATCTTTATGTAGGGACAAGCATCTATTTTGTTTGGGGTATACTGCTTTCTTATC TCGCCACCCTATTTGCTAGTTTTTCTTGTTTGGTTGTGTGGTGTTGTCGCAAGCAATATAGGGGAGCTCT TGTTTGGCTTCCAGGGGTTTGGGTGGCGATAGAAGCAATACGCTATTATGGGTTGCTTTCAGGAGTTTCT TTTGATTTTATTGGCTGGCCTCTTACAGCGACAGCCTATGGCCGGCAATTCGGCAGCTTTTTTGGATGGG CTGGACAAAGCTTTCTAGTTATTGCTGCCAATATATGCTGTTTTGCAGTATGTTTATTAAAACACTCTTT TTCCAAAGGTTTGTGGTTGACGTTGTGCGCGTTCCCTTATCTGTTAGGCGGAGCGCATTACGAATACCTA AAGAAGCATTTTTCCGACTCTGAAGTGCTTCGAGTTGCCATCGTGCAGCCTGGATATAGTCCTCATATGC ATGCAGGGAGGACGGCTAGTGCTATTTGGAGAGGTTTGGTTTCTTTGTGCCAGACTATTCAAACTCCTGT AGATGTGATCGTTTTCCCAGAAGTAAGTGTTCCTTTTGGCTTACATAGACAAGCCTATACTCTTCATGAA AATCAGCCTGTATTAGAAAGTTTGCTTCCTAACAAATCTTGGGGCGAGTTTTTCACAAATTTGGATTGGA TCCAAGCGATAGCTGAACGTTATCAATGCACCGTTATCATGGGAATGGAACGATGGGAAAATAAAGGGGG AATACTGCATTTGTATAATGCTGCTGAATGCGTATCGCGAGAAGGGGAAATAACTAGCTATGATAAGCGG ATTCTTGTTCCTGGAGGTGAGTACATCCCTGGAGGGAAAATAGGTTTTTCCTTGTGTCAAACCTTTTTCC CAGAATTTGCTCTTCCCTTTCAACGTTTGCCAGGAGAGTTTTCTGGAGTTGTGAATATAACAGAGCGAAT AAAAGCTGGGATCTCTATTTGTTATGAGGAGACATTTGGGTATGCAATTCGCCCTTACAAAAGGCAACAA GCCGATATTTTAGTAAATCTTACTAATGACGGTTGGTATCCGCGTTCAAGGCTGCCTCTAGTACATTTTT ATCATGGCATGTTACGTAATCAAGAGTTGGGTATACCTTGTATTCGCGCCTGTCGCACAGGAGTTTCTGC TGCAGTGGATTCTTTGGGTAGAATTGTCGGCATACTTCCCTGGGAATCGAGAACTTGCCCAGTTTCTACA GGAGTACTCCAAGTTTCCGTCCCTCTTTACAGTTATCATACTGTATATGCAAGGCTGGGTGATGCTCCTC TGTTACTGATTGCAGTTTGTTCGGTTATCGGAGCGATTGCCTATTTTTATAGGAAAAAGAAAGAGACCCC ACCACAAACATTTTTTTGA >gi|3328612|gb|AAC67797.1| Fructose-6-P Phosphotransferase [Chlamydia trachomatis][SEQ ID NO:61] MSSNKHASLCQKTPSLCRELQKAPALLLTEDIRFKALLNERIDSVAELFPCTYNSPYYKFISKSDLSAET SPLKVGVMLSGGPAPGGHNVILGLLHSIKKLHPNSQLLGFIRNGEGLLNNNTVEITDEFIEEFRNSGGFN CIGTGRTNIITEENKARCLQTANELDLDGLVIIGGDGSNTATAILAEYFAKHQAKTVLVGVPKTIDGDLQ HLFLDLTFGFDTATKFYSSIISNISRDALSCKGHYHFIKLMGRSSSHITLECALQTHPNIALIGEEIAEK SISLETLIHDICETIADRAAMGKYHGVILIPEGVIEFIPEIQSLVKEIESIPEQENLYQALSLSSQQLLC QFPEDICHQLLYNRDAHGNVYVSKISVDKLLIHLVRQHLETHFRQVPFNAISHFLGYEGRSGTPTHFDNV YSYNLGYGAGVLVFNRCNGYLSTIEGLTSPIEKWRLRALPIVRMLTTKQGKDSKHYPLIKKRLVDIASPV FNKFSLYRKIWALEDSYRFVGPLQIHSPEDAHSDDFPPLILFLNHNEWQKRCSICLEIPDQDY >gi|3328609|gb|AE001294.1:2452-4113[SEQ ID NO:62] ATGTCGTCGAATAAACATGCTTCTCTTTGTCAAAAGACGCCTTCTTTGTGTCGGGAGCTTCAAAAAGCTC CTGCTCTTCTATTAACAGAAGACATAAGGTTTAAAGCTCTTCTTAATGAACGCATTGACTCTGTTGCAGA ACTATTTCCATGCACTTATAACTCTCCCTACTACAAATTTATTTCGAAGTCCGATCTTTCCGCTGAGACC TCTCCCCTTAAAGTGGGCGTTATGCTTTCTGGAGGCCCAGCTCCTGGTGGGCACAATGTCATCTTAGGAT TGCTACACAGTATTAAAAAGCTCCATCCGAATAGTCAGCTTTTAGGATTTATTCGCAATGGAGAAGGACT TCTCAATAATAATACTGTAGAAATCACAGATGAATTCATTGAAGAGTTTCGTAACTCTGGAGGCTTTAAT TGCATAGGAACAGGTCGCACTAATATCATAACCGAAGAAAATAAAGCGCGCTGTTTACAAACAGCAAATG AACTCGATTTAGATGGATTAGTGATTATTGGAGGCGATGGTTCGAATACAGCCACGGCGATTCTTGCTGA ATATTTTGCTAAGCATCAAGCAAAAACGGTATTAGTTGGTGTTCCCAAAACTATTGATGGAGATTTGCAG CACCTATTTTTAGACCTCACATTTGGGTTTGATACTGCTACTAAATTTTATTCATCCATCATCAGCAACA TTTCTAGAGACGCATTATCGTGTAAAGGCCACTATCATTTTATTAAACTAATGGGCCGGTCTTCTTCTCA TATCACGCTAGAATGCGCACTACAGACTCACCCAAATATTGCTCTTATAGGCGAAGAGATTGCAGAAAAA AGCATCTCCTTAGAAACATTAATCCATGATATTTGTGAAACAATAGCAGATCGAGCTGCTATGGGGAAAT ACCATGGCGTTATTCTCATCCCTGAAGGAGTCATTGAGTTTATTCCTGAAATACAGTCTCTGGTTAAAGA AATTGAATCCATTCCAGAGCAGGAGAATCTTTACCAAGCTTTATCCTTATCTTCTCAGCAACTTTTATGC CAATTTCCGGAAGATATTTGCCATCAGCTCTTGTATAATAGAGATGCTCATGGCAACGTCTATGTATCAA AAATTAGTGTTGATAAACTTCTGATTCATCTAGTTCGTCAACATTTAGAAACACATTTTAGACAAGTTCC CTTCAATGCAATCTCCCATTTTTTAGGTTATGAAGGGCGTTCAGGAACTCCTACACATTTTGATAATGTG TATAGCTATAACTTAGGATATGGTGCTGGGGTTCTCGTTTTTAACCGCTGTAATGGGTATTTATCCACGA TCGAAGGTCTAACTAGCCCTATTGAAAAATGGCGATTGCGCGCTTTACCCATTGTTCGAATGTTGACGAC CAAGCAGGGGAAAGACAGTAAACATTATCCTCTGATAAAAAAAAGATTGGTAGATATTGCTAGTCCTGTT TTTAATAAGTTCTCACTGTATCGGAAAATCTGGGCTTTAGAAGACTCCTATCGCTTTGTAGGGCCATTAC AAATACATTCTCCGGAGGATGCTCATTCTGATGATTTTCCTCCTTTAATTTTGTTTTTGAATCATAATGA ATGGCAAAAACGCTGTTCTATTTGTTTAGAAATCCCCGATCAGGATTATTAA >gi|3328517|gb|AAC67709.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO:63] MICCDKVLSSVQSMPVIDKCSVTKCLQTAKQAAVLALSLFAVFASGSLSILSAAVLFSGTAAVLPYLLIL TTALLGFVCAVIVLLRNLSAVVQSCKKRSPEEIEGAARPSDQQESGGRLSEESASPQASPTSSTFGLESA LRSIGDSVSGAFDDINKDNSRSRSHSF >gi|3328516|gb|AE001286.1:75-578[SEQ ID NO:64] ATGATCTGCTGTGACAAAGTCTTGTCGAGCGTACAATCAATGCCTGTTATAGATAAATGCTCTGTAACGA AATGCTTACAAACGGCTAAGCAAGCAGCTGTTCTTGCGTTGTCTTTGTTTGCGGTGTTTGCTTCAGGAAG TTTATCCATATTATCAGCGGCGGTACTGTTTAGTGGCACTGCTGCTGTTCTTCCATATCTGCTGATATTA ACAACAGCTCTTCTAGGATTTGTTTGTGCTGTTATTGTGCTTTTAAGAAATTTATCAGCAGTTGTTCAGA GTTGTAAAAAGAGATCACCTGAAGAAATTGAAGGGGCTGCTCGTCCCTCTGATCAGCAGGAATCAGGAGG ACGTTTGTCCGAGGAGAGCGCTTCACCACAAGCATCTCCTACTTCGTCTACTTTTGGTCTTGAATCCGCT TTGCGCTCAATAGGAGATA >gi|3328482|gb|AAC67677.1| L28 Ribosomal Protein [Chlamydia trachomatis][SEQ ID NO:65] MSKKCALTGRKPRRGYSYAIRGISKKKKGIGLKVTGRTKRRFFPNMMTKRLWSTEENRFLKLKISAAALR LVDKLGLDQVVARAKSKGF >gi|3328480|gb|AE001283.1:c2251-1982[SEQ ID NO:66] ATGTCGAAAAAATGTGCGCTTACAGGAAGAAAGCCTCGTCGCGGTTATAGCTATGCTATCCGAGGGATTT CTAAAAAGAAAAAAGGGATCGGTTTGAAAGTTACAGGAAGAACAAAACGTCGATTCTTCCCTAATATGAT GACTAAGAGACTATGGTCTACTGAGGAAAATCGCTTCCTCAAACTCAAAATTTCTGCAGCAGCTTTACGC CTTGTTGATAAACTAGGGTTAGATCAGGTTGTTGCTAGAGCTAAAAGCAAGGGTTTTTAG >gi|3328436|gb|AAC67635.1| SS DNA Binding Protein [Chlamydia trachomatis][SEQ ID NO:67] MLFGYLVGFLAADPEERMTSGGKRVVVLRLGVKSRVGSKDETVWCRCNIWNNRYDKMLPYLKKGSSVIVA GELSLESYVGRDGSPQASISVSVDTLKFNSGSSRPDARGSDEGRQRANDNVSIGFDGESLDTDSALDKEV YAGFGEDQQYASEDVPF >gi|3328434|gb|AE001279.1:1060-1533[SEQ ID NO:68] ATGTTGTTCGGATATTTGGTAGGATTTCTAGCTGCCGATCCTGAAGAAAGAATGACATCCGGAGGTAAAC GGGTTGTTGTTTTACGTTTGGGTGTAAAATCTCGTGTAGGATCTAAAGATGAAACAGTGTGGTGCAGATG CAATATCTGGAACAACCGTTATGATAAGATGCTTCCTTATTTGAAGAAAGGTTCTTCAGTCATTGTTGCT GGAGAGCTTTCTTTAGAAAGCTATGTAGGTAGAGACGGTTCTCCACAAGCTTCTATTTCTGTAAGCGTAG ATACATTAAAATTTAATTCCGGATCTTCTCGTCCTGATGCTAGAGGTTCAGATGAAGGTCGTCAGAGAGC TAATGATAATGTCTCTATTGGATTTGATGGAGAAAGTTTAGATACAGACTCTGCGCTTGATAAGGAAGTC TATGCAGGGTTTGGAGAAGACCAACAGTATGCTAGTGAGGATGTTCCTTTTTAG >gi|3328411|gb|AAC67611.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO:69] MKKQEKMHPQNLLKVFIFFLAFFISYPSAEAHSPLQSSIQEKILTARPGDYAVLSRGSQKFFFLIRQSSS EATWVEMSEFASLTQQEKKLVEQSSWKNAFHQLQSSKKVYLLRISKNPLMIFVLKNAQWMPLSEKDPLPF FVKILRLPLSPAPSHLIKYKGKERTPWSPRTSLNGELITLPSSAWISVWPKDSSPLSEKNILIYFSNNER LAFPLWTSIDTPTGTVIIKTIEMGHQAASSYPALPNF >gi|3522886|gb|AE001277.1:c6191-5448[SEQ ID NO:70] ATGAAAAAGCAAGAAAAAATGCACCCTCAAAACCTTCTTAAAGTTTTTATTTTTTTCTTGGCATTTTTCA TATCCTATCCCTCGGCTGAAGCCCATTCTCCTCTCCAATCATCAATCCAAGAAAAAATTCTAACTGCCCG CCCCGGAGACTATGCCGTCTTAAGCCGAGGATCTCAAAAATTTTTCTTTTTAATTCGCCAAAGTTCTTCG GAAGCGACTTGGGTCGAAATGTCTGAATTTGCCTCCCTAACACAGCAAGAAAAAAAATTAGTAGAACAGT CTTCCTGGAAGAATGCCTTCCATCAACTCCAATCTTCAAAAAAAGTGTACTTGTTACGAATTTCCAAAAA TCCTCTTATGATTTTTGTTCTCAAAAATGCGCAATGGATGCCTCTCTCAGAAAAAGATCCTTTGCCTTTC TTTGTAAAAATCCTTCGACTCCCTTTATCTCCAGCCCCCTCTCACTTAATTAAATACAAAGGGAAAGAAC GCACCCCCTGGTCTCCGCGAACATCTTTGAATGGAGAACTCATAACCCTTCCTTCCAGTGCTTGGATTTC TGTTTGGCCAAAAGATTCTTCTCCTCTATCAGAAAAAAATATTCTCATATATTTTTCTAACAATGAACGT TTAGCGTTTCCTCTATGGACTAGTATTGATACTCCTACAGGGACAGTGATTATTAAGACTATTGAAATGG GGCACCAAGCCGCCTCCTCCTATCCAGCTCTTCCCAATTTCTAG crpA, CHLTR 15 kD cysteine-rich protein (Chlamydia trachomatis serovar D (D/UW- 3/Cx) DNA sequence[SEQ ID NO:71] AATATGAGCACTGTACCCGTTGTTCAAGGAGCTGGATCTTCCAATTCGGCACAGGATATTTCCACTAGACCAT TAACACTGAAAGAGCGTATATCGAATCTTCTATCTTCCACTGCATTTAAGGTGGGATTAGTGGTGATAGGACT ACTTTTGTGATTGCTACTTTGATATTCCTAGTTTCGGCAGCTTCGTTTGTAAATGCCATCTATCTAGTAGCTA TTCCTGCTATTTGGGATGCGTGAATATCTGCGTAGGAATTTTATCCATGGAAGGACACTGTTCTCCGGAGAGA TGGATCTTATGTAAGAAGTATTAAAGACTTCAGAAGATATCATCGATGATGGGCAGATAAACAACTCTAATAA AGTGTTTACTGATGAGAGGTTGAAGCCATAGGTGGGGTAGTGGAATCTCTATCTAGAAGAAATAGTCTGGTGG ATCAGACCCAATGA Translated amino acid sequence[SEQ ID NO:72]      NMS TVPVVQGAGS SNSAQDISTR PLTLKERISN LLSSTAFKVG LVVIGLLLVI ATLIFLVSAA SFVNAIYLVA IPAILGCVNI CVGILSMEGH CSPERWILCK KVLKTSEDII DDGQINNSNK VFTDERLNAI GGVVESLSRR NSLVDQTQ* OmcA, CHLTR 9 kD cystein-rich outer membrane complex lipoprotein (Chlamydia trachomatis serovar D (D/UW-3/Cx) DNA sequence[SEQ ID NO:73] GGGCTAGTTTCTTTTATTGTTAAAAGAATTGCTTTTATCGATAAAAGAAACTTCAAGAGCCCTTTTCTAG AAAGGAGTCTGGAAGTTATGAAAAAAACTGCTTTACTCGCTGCTTTATGTAGTGTTGTTTCTTTAAGTAG TTGTTGTCGATCGTTGACTGTTGCTTCGAAGATCCATGCGCACCTATCCAATGTTCACCTTGTGAATCTA AGAAGAAAGACGTAGACGTGGTTGCAACTCTTGTAACGGGTATGTCCCAGCTTGCAAACCTTGCGGAGGG GATACGCACCAAGATGCTAAACATGGCCTCAAGCTAGAGGAATTCCAGTTGACGGCAAATGCAGACAATA G Translated amino acid sequence[SEQ ID NO:74]                               GL VSFIVKRIAF IDKRNFKSPF LERSLEVMKK TALLAALCSV VSLSSCCRIV DCCFEDPCAP IQCSPCESKK KDVDGGCNSC NGYVPACKPC GGDTHQDAKH GPQARGIPVD GKCRQ* cutE, apolipoprotein N-acyltransferase (Chlamydia trachomatis serovar D (D/UW- 3/Cx) DNA sequence[SEQ ID NO:75] GCTAGTAAGGGAGCCCCTTTAGTGTTTAAACTTGTGTCATACATCATCCTTTCTTGGGTGCTGGTCTGTTTG GCTCAGCCGGATGTAAGTGTTGTAGCTTCTGTTGTTAGTTGTATTTGCGGTTACAGCTTACTTTGGGCTGGG CTTTTTGTTTAGTAGAGCAATTATCTTGGAAGAAAGTTTGGTGCATCGCTTTTATTTGCACTTGGACTGTCG AAGGCGCTCATTTCCTTGGATGCTTGAAGATCTTTATGTAGGGACAAGCATCTATTTTGTTTGGGGTATACT GCTTTCTTATCTCGCCACCCTTTTGCTAGTTTTTCTTGTTTGGTTGTGTGGTGTTGTCGCAAGCAATATAGG GGAGCTCTTGTTTGGCTTCCAGGGGTTTGGTGGCGATAGAAGCAATACGCTATTATGGGTTGCTTTCAGGAG TTTCTTTTGATTTTATTGGCTGGCCTCTTACAGCGCAGCCTATGGCCGGCAATTCGGCAGCTTTTTTGGATG GGCTGGACAAAGCTTTCTAGTTATTGCTGCCAATATATGCTGTTTGCAGTATGTTTATTAAAACACTCTTTT TCCAAAGGTTTGTGGTTGACGTTGTGCGCGTTCCCTTATCTGTTAGGCGAGCGCATTACGATACCTAAATGA AGCATTTTTCCGACTCTGAAGTGCTTCGAGTTGCCATCGTGCAGCCTGGATATAGTCTCATATGCATGCAGG GAGGACGGCTAGTGCTATTTGGAGAGGTTTGGTTTCTTTGTGCCAGACTATTCAAACTCCTGTGATGTGATC GTTTTCCCAGAAGTAAGTGTTCCTTTTGGCTTACATAGACAAGCCTATACTCTTCATGAAAATCAGCCTGAT TAGAAAGTTTGCTTCCTAACAAATCTTGGGGCGAGTTTTTCACAAATTTGGATTGGATCCAAGCGATAGCTG AACGTATCAATGCACCGTTATCATGGGAATGGAACGATGGGAAAATAAAGGGGGAATACTGCATTTGTATAA TGCTGCTGAATGGTATCGCGAGAAGGGGAAATAACTAGCTATGATAAGCGGATTCTTGTTCCTGGAGGTGAG TACATCCCTGGAGGGAAAAAGGTTTTTCCTTGTGTCAAACCTTTTTCCCAGAATTTGCTCTTCCCTTTCAAC GTTTGCCAGGAGAGTTTTCTGGAGTTTGAATATAACAGAGCGAATAAAAGCTGGGATCTCTATTTGTTATGA GGAGACATTTGGGTATGCAATTCGCCCTTACAAAGGCAACAAGCCGATATTTTAGTAAATCTTACTAATGAC GGTTGGTATCCGCGTTCAAGGCTGCCTCTAGTACATTTTTTCATGGCATGTTACGTAATCAAGAGTTGGGTA TACCTTGTATTCGCGCCTGTCGCACAGGAGTTTCTGCTGCAGTGGATCTTTGGGTAGAATTGTCGGCATACT TCCCTGGGAATCGAGAACTTGCCCAGTTTCTACAGGAGTACTCCAAGTTTCCGTCCTCTTTACAGTTATCAT ACTGTATATGCAAGGCTGGGTGATGCTCCTCTGTTACTGATTGCAGTTTGTTCGGTTATCGAGCGATTGCCT ATTTTTATAGGAAAAAGAAAGAGACCCCACCACAAACATTTTTTTGA Translated amino acid sequence[SEQ ID NO:76]                                                   ASKG APLVFKLVSY IILSWVLVCL AQPDVSVVAS VVSCICGYSL LWAGLFALVE QLSWKKVWCI AFIWTWTVEG AHFSWMLEDL YVGTSIYFVW GILLSYLATL FASFSCLVVW CCRKQYRGAL VWLPGVWVAI EAIRYYGLLS GVSFDFIGWP LTATAYGRQF GSFFGWAGQS FLVIAANICC FAVCLLKHSF SKGLWLTLCA FPYLLGGAHY EYLKKHFSDS EVLRVAIVQP GYSPHMHAGR TASAIWRGLV SLCQTIQTPV DVIVFPEVSV PFGLHRQAYT LHENQPVLES LLPNKSWGEF FTNLDWIQAI AERYQCTVIM GMERWENKGG ILHLYNAAEC VSREGEITSY DKRILVPGGE YIPGGKIGFS LCQTFFPEFA LPFQRLPGEF SGVVNITERI KAGISICYEE TFGYAIRPYK RQQADILVNL TNDGWYPRSR LPLVHFYHGM LRNQELGIPC IRACRTGVSA AVDSLGRIVG ILPWESRTCP VSTGVLQVSV PLYSYHTVYA RLGDAPLLLI AVCSVIGAIA YFYRKKKETP PQTFF* pal, peptidoglycan-associated lipoprotein (Chlamydia trachomatis serovar D (D/UW- 3/Cx) DNA sequence[SEQ ID NO:77]                                G AAAATTGTTA TAGGATCAGG AGAGAAACGT TTCCCATGCT GGGGAGCATT TCCTTCACAA CATATAAAGA AAACCTCATG AGAAAGACTA TTTTTAAAGC GTTTAATTTA TTATTCTCCC TTCTTTTTCT TTCTTCATGC TCTTATCCTT GCAGAGATTG GGAATGCCAT GGTTGCGACT CCGCAAGACC TCGTAAATCC TCTTTTGGAT TCGTACCTTT CTACTCCGAT GAAGAAATTC AACAAGCTTT TGTTGAAGAT TTTGATTCCA AAGAAGAGCA GCTGTACAAA ACGAGCGCAC AGAGTACCTC TTTCCGAAAT ATCACTTTCG CTACAGATAG TTATTCTATT AAAGGAGAGG ATAACCTCAC GATTCTTGCA AGCTTAGTTC GTCATTTGCA TAAATCTCCT AAAGCTACGC TATATATAGA GGGCCATACA GATGAACGTG GAGCTGCAGC TTATAACCTA GCTTTAGGAG CTCGTCGTGC GAATGCTGTA AAACAATACC TCATCAAACA GGGAATCGCT GCAGACCGCT TATTCACTAT TTCTTACGGA AAAGAACATC CTGTTCATCC AGGCCATAAT GAATTAGCTT GGCAACAAAA TCGTCGTACT GAATTTAAGA TCCATGCTCG CTAA Translated amino acid sequence[SEQ ID NO:78]                                                ENCYRIR RETFPMLGSI SFTTYKENLM RKTIFKAFNL LFSLLFLSSC SYPCRDWECH GCDSARPRKS SFGFVPFYSD EEIQQAFVED FDSKEEQLYK TSAQSTSFRN ITFATDSYSI KGEDNLTILA SLVRHLHKSP KATLYIEGHT DERGAAAYNL ALGARRANAV KQYLIKQGIA ADRLFTISYG KEHPVHPGHN ELAWQQNRRT EFKIHAR*

[0028] The following Chlamydia trachomatis outer membrane proteins (full sequences above) are disclosed for the first time as being useful in a C. trachomatis vaccine. A vaccine comprising one or more of these proteins (or native or functional analogues thereof) is a further aspect of this invention (particularly in the context of being presented on the surface of a bleb). >gi|6578118|gb|AAC68456.2| predicted Protease containing IRBP and DHR domains [Chlamydia trachomatis][SEQ ID NO: 3] >gi|6578109|gb|AAC68227.2| CHLPN 76kDa Homolog [Chlamydia trachomatis][SEQ ID NO: 5] >gi|3328866|gb|AAC68034.1| Sulfite Reductase [Chlamydia trachomatis][SEQ ID NO: 19] >gi|3328815|gb|AAC67986.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO: 23] >gi|3328587|gb|AAC67774.1| CMP-2-keto-3-deoxyoctulosonic acid synthetase [Chlamydia trachomatis][SEQ ID NO: 27] >gi|3329039|gb|AAC68197.1| Thio: disulfide Interchange Protein [Chlamydia trachomatis][SEQ ID NO: 29] >gi|3329000|gb|AAC68161.1| Yop proteins translocation lipoprotein J [Chlamydia trachomatis][SEQ ID NO: 31] >gi|3328905|gb|AAC68071.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO: 33] >gi|3328884|gb|AAC68051.1| Phosphatidate Cytidylytransferase [Chlamydia trachomatis][SEQ ID NO: 35] >gi|3328855|gb|AAC68022.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO: 37] >gi|3328772|gb|AAC67946.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO: 39] >gi|3328763|gb|AAC67938.1| O-Sialoglycoprotein Endopeptidase family [Chlamydia trachomatis][SEQ ID NO: 49] >gi|6578102|gb|AAC67897.2| ATP Synthase Subunit K [Chlamydia trachomatis][SEQ ID NO: 51] >gi|3329252|gb|AAC68382.1| S14 Ribosomal Protein [Chlamydia trachomatis][SEQ ID NO: 53] >gi|3328987|gb|AAC68150.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO: 57] >gi|3328972|gb|AAC68136.1| Apolipoprotein N-Acetyltransferase [Chlamydia trachomatis][SEQ ID NO: 59] >gi|3328612|gb|AAC67797.1| Fructose-6-P Phosphotransferase [Chlamydia trachomatis][SEQ ID NO: 61] >gi|3328517|gb|AAC67709.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO: 63] >gi|3328482|gb|AAC67677.1| L28 Ribosomal Protein [Chlamydia trachomatis][SEQ ID NO: 65] >gi|3328436|gb|AAC67635.1| SS DNA Binding Protein [Chlamydia trachomatis][SEQ ID NO: 67] >gi|3328411|gb|AAC67611.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO: 69]

[0029] Amino Acid Sequences:

[0030] >gi|6578118|gb|AAC68456.2| predicted Protease containing IRBP and DHR domains [Chlamydia trachomatis][SEQ ID NO:3]

[0031] >gi|6578109|gb|AAC68227.2| CHLPN 76 kDa Homolog [Chlamydia trachomatis][SEQ ID NO:5]

[0032] >gi|3328866|gb|AAC68034.1| Sulfite Reductase [Chlamydia trachomatis][SEQ ID NO:19]

[0033] >gi|3328815|gb|AAC67986.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO:23]

[0034] >gi|3328587|gb|AAC67774.1| CMP-2-keto-3-deoxyoctulosonic acid synthetase [Chlamydia trachomatis][SEQ ID NO:27]

[0035] >gi|3329039| gb|AAC68197.1| Thio:disulfide Interchange Protein [Chlamydia trachomatis][SEQ ID NO:29]

[0036] >gi|3329000|gb|AAC68161.1| Yop proteins translocation lipoprotein J [Chlamydia trachomatis][SEQ ID NO:31]

[0037] >gi|3328905|gb|AAC68071.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO:33]

[0038] >gi|3328884|gb|AAC68051.1| Phosphatidate Cytidylytransferase [Chlamydia trachomatis][SEQ ID NO:35]

[0039] >gi|3328855|gb|AAC68022.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO:37]

[0040] >gi|3328772|gb|AAC67946.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO:39]

[0041] >gi|3328763|gb|AAC67938.1| O-Sialoglycoprotein Endopeptidase family [Chlamydia trachomatis][SEQ ID NO:49]

[0042] >gi|6578102|gb|AAC67897.2| ATP Synthase Subunit K [Chlamydia trachomatis][SEQ ID NO:51]

[0043] >gi|3329252|gb|AAC68382.1| S14 Ribosomal Protein [Chlamydia trachomatis][SEQ ID NO:53]

[0044] >gi|3328987|gb|AAC68150.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO:57]

[0045] >gi|3328972|gb|AAC68136.1| Apolipoprotein N-Acetyltransferase [Chlamydia trachomatis][SEQ ID NO:59]

[0046] >gi|3328612|gb|AAC67797.1| Fructose-6-P Phosphotransferase [Chlamydia trachomatis][SEQ ID NO:61]

[0047] >gi|3328517|gb|AAC67709.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO:63]

[0048] >gi|3328482|gb|AAC67677.1| L28 Ribosomal Protein [Chlamydia trachomatis][SEQ ID NO:65]

[0049] >gi|3328436|gb|AAC67635.1| SS DNA Binding Protein [Chlamydia trachomatis][SEQ ID NO:67]

[0050] >gi|3328411|gb|AAC67611.1| hypothetical protein [Chlamydia trachomatis][SEQ ID NO:69]

[0051] Again, when such blebs are present in a vaccine formulation they may be more protective against Chlamydia trachomatis infection than the use of the protein in isolation.

[0052] Particularly beneficial pairs of Chlamydia trachomatis antigens are further preferred embodiments of this invention. Thus in a further aspect a Gram-negative bleb (preferably from gonococcus) is provided presenting on its surface both the PorB and PmpG outer membrane proteins from Chlamydia trachomatis. Furthermore, a Gram-negative bleb (preferably from gonococcus) is provided presenting on its surface both the PorB and MOMP (from one or more serovars) outer membrane proteins from Chlamydia trachomatis. Lastly, a Gram-negative bleb (preferably from gonococcus) is provided presenting on its surface both the PmpG and MOMP (from one or more serovars) outer membrane proteins from Chlamydia trachomatis.

[0053] By MOMP (or OMP1 or OMP I) from one or more (1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more) serovars it is preferred that one or more should be selected from a list serovars consisting of: B, Ba, D, Da, E, L1, L2, L2a, F, G, K, L3, A, C, H, I, Ia, & J; more preferably from a list consisting of D, E, F, G, K, H, I, & J. Most preferably one or more MOMPs should at least comprise MOMP from serovar D or E (most preferably D). A further preferred strategy is the selection of one or more MOMP from each of the following 3 serogroups: B-serogroup (consisting of serovars B, Ba, D, Da, E, L1, L2 and L2a, and preferably selected from serovars D, Da, & E); F-G-serogroup (consisting of serovars F and G); and C-serogroup (consisting of servars A, C, H, I, Ia, J, K, and L3, and preferably selected from serovars H, I, Ia, J and K).

[0054] Most preferably the genes for the Chlamydia trachomatis antigens should be inserted at the PorA locus of Neisseria (preferably gonococcus).

[0055] Such a prepartion formulated as a vaccine may give enhanced protection to a host against Chlamydia trachomatis than when a single antigen is administered.

[0056] Preferably the bleb has been derived from a strain (preferably gonococcus) which has been modified to upregulate one or more protective outer membrane antigens (as described below).

[0057] Preferably the bleb has been derived from a strain (preferably gonococcus) which has been modified to downregulate one or more immunodominant variable or non-protective outer membrane antigens (as described below).

[0058] Preferably the blebs are derived from a strain (preferably gonococcus) which has a detoxified lipid A portion of bacterial LPS, due to the strain having been engineered to reduce or switch off expression of one or more genes which cause LPS to be toxic (preferably selected from the following genes, or homologues thereof htrB, msbB and lpxK; see section below).

[0059] Preferably the blebs are derived from a strain (preferably gonococcus) which has a detoxified lipid A portion of bacterial LPS, due to the strain having been engineered to express at a higher level of one or more genes producing a gene product that is capable of detoxifying LPS (preferably selected from the following genes, or homologues thereof: pmrA, pmrB, pmrE and pmrF; see section below).

[0060] Vaccine compositions comprising the bleb of the invention and a pharmaceutically suitable excipient or carrier is also envisaged. Preferably the vaccine additionally comprises a mucosal adjuvant. Mucosal adjuvants are well known in the art (see Vaccine Design “The subunit and adjuvant approach” (eds Powell M. F. & Newman M. J.) (1995) Plenum Press New York). A preferred mucosal adjuvant is LT2 (or LTII, which can be split into LTIIa and LTIIb—see Martin et al. Infection and Immunity, 2000, 68:281-287). Preferably such vaccines should be formulated and administered as described below in “vaccine formulations”.

[0061] The content of blebs per dose in the vaccine will typically be in the range 1-100 μg, preferably 5-50 μg, most typically in the range 5-25 μg.

[0062] Optimal amounts of components for a particular vaccine can be ascertained by standard studies involving observation of appropriate immune responses in subjects. Following an initial vaccination, subjects may receive one or several booster immunisations adequately spaced.

[0063] A method of preventing Chlamydia trachomatis infection in a host is also provided comprising the steps of administering an effective amount of the above vaccine to a host in need thereof. Preferably the vaccine is mucosally administered via either a intranasal, oral, intradermal or intravaginal route.

[0064]Chlamydia pneumoniae Antigens Integrated into a Gram Negative Bacterial bleb

[0065] In a further aspect, the invention provides a Gram-negative bleb presenting on its surface a protective antigen from Chlamydia pneumoniae. Neisseria meningitidis, Moraxella catharralis, and Haemophilus influenzae are preferred species for the production of said bleb. A bacterial strain capable of producing such a bleb is a further aspect of the invention. Such protective antigens are preferably one or more of those listed below:

[0066] 1) Cell Envelope: Membrane Proteins, Lipoproteins and Porins Gene: Protein Function: yaeT OMP85 homolog 60IM 60 kD inner membrane protein lgt prolipoprotein diacylglyceryl transferase crpA CHLTR 15 kD cysteine-rich protein omcB 60 kD cysteine-rich outer membrane complex protein omcA 9 kD cysteine-rich outer membrane complex lipoprotein cutE apolipoprotein N-acetyltransferase ompA major outer membrane protein pal peptidoglycan-associated lipoprotein porB outer membrane protein analog

[0067] 2) Coding Genes (Not in C. trachomatis) Gene: Protein Function: yqfF conserved hypothetical inner membrane protein yxjG hypothetical protein guaA GMP synthase guaB inosine 5′-monophosphate dehydrogenase argR similarity to arginine repressor CPn0232 similarity to 5′-methylthioadenosine nucleosidase CPn0251 conserved hypothetical protein CPn0278 conserved outer membrane lipoprotein protein/a> CPn0279 possible ABC transporter permease yxjG hypothetical protein yqeV hypothetical protein CPn0486 hypothetical proline permease CPn0505 3-methyladenine DNA glycosylase CPn0562 CHLPS 43 kDa protein CPn0585 similarity to CHLPS IncA yvyD conserved hypothetical protein CPn0608 uridine 5′-monophosphate synthase CPn0735 uridine kinase CPn0907 CutA-like periplasmic divalent cation tolerance protein CPn0927 CHLPS 43 kDa protein CPn0928 CHLPS 43 kDa protein CPn0929 CHLPS 43 kDa protein CPn0980 similar to S. cerevisiae 52.9 kDa protein bioA adenosylmethionine-8-amino-7-oxononanoate aminotransferase bioD dethiobiotin synthetase bioB biotin synthase CPn1045 conserved hypothetical membrane protein CPn1046 tryptophan hydroxylase

[0068] 3) Chlamydia-Specific Proteins Gene: Protein Function: pmp_1 polymorphic outer membrane protein pmp_2 polymorphic outer membrane protein pmp_3 polymorphic outer membrane protein pmp_3 polymorphic outer membrane protein pmp_4 polymorphic outer membrane protein pmp_4 polymorphic outer membrane protein pmp_5 polymorphic outer membrane protein pmp_5 polymorphic outer membrane protein CPn0133 CHLPS hypothetical protein CPn0186 similarity to IncA incB inclusion membrane protein B incC inclusion membrane protein C CPn0332 CHLTR T2 protein ltuB LtuB protein pmp_6 polymorphic outer membrane protein pmp_7 polymorphic outer membrane protein pmp_8 polymorphic outer membrane protein pmp_9 polymorphic outer membrane protein pmp_10 polymorphic outer membrane protein pmp_10 polymorphic outer membrane protein pmp_11 polymorphic outer membrane protein pmp_12 polymorphic outer membrane protein pmp_13 polymorphic outer membrane protein pmp_14 polymorphic outer membrane protein pmp_15 polymorphic outer membrane protein pmp_16 polymorphic outer membrane protein pmp_17 polymorphic outer membrane protein pmp_17 polymorphic outer membrane protein pmp_17 polymorphic outer membrane protein pmp_18 polymorphic outer membrane protein pmp_19 polymorphic outer membrane protein pmp_20 polymorphic outer membrane protein euo CHLPS Euo protein CPn0562 CHLPS 43 kDa protein homolog CPn0585 similar to CHLPS inclusion membrane protein A CPn0728 CHLPN 76 kDa protein homolog CPn0729 CHLPN 76 kDa protein homolog gp6D CHLTR plasmid protein CPn0927 CHLPS 43 kDa protein homolog CPn0928 CHLPS 43 kDa protein homolog CPn0929 CHLPS 43 kDa protein homolog pmp_21 polymorphic outer membrane protein ltuA LtuA protein

[0069] (Full sequence information has been published at the Chlamydia Genome Project web site: http://chlamydia-www.berkeley.edu:4231/index.html).

[0070] Additional Chlamydia Genes, and Encoded Proteins, Suitable for Expression in a Gram-Negative Bacteria for OMV Vaccine Preparation: Chlamydia pneumoniae 98 kD putative outer membrane WO2000262 protein gene. 37-A2 Patent InventorsDUNN PL OOMEN RP MURDIN AD Chlamydia POMP91B precursor gene. WO2000262 Patent InventorsDUNN PL 39-A2 OOMEN RP MURDIN AD Chlamydia antigen CPN100634 full length coding WO2000327 sequence. 94-A2 Patent InventorsOOMEN RP WANG J MURDIN AD Chlamydia antigen CPN100634 gene open reading frame. WO2000327 Patent InventorsOOMEN RP 94-A2 WANG J MURDIN AD Chlamydia antigen CPN100635 full length coding WO2000327 sequence. 94-A2 Patent InventorsOOMEN RP WANG J MURDIN AD Chlamydia antigen CPN100635 gene open reading frame. WO2000327 Patent InventorsOOMEN RP 94-A2 WANG J MURDIN AD Chlamydia antigen CPN100638 full length coding WO2000327 sequence. 94-A2 Patent InventorsOOMEN RP WANG J MURDIN AD Chlamydia antigen CPN100638 gene open reading frame. WO2000327 Patent InventorsOOMEN RP 94-A2 WANG J MURDIN AD Chlamydia antigen CPN100639 full length coding WO2000327 sequence. 94-A2 Patent InventorsOOMEN RP WANG J MURDIN AD Chlamydia antigen CPN100639 gene open reading frame. WO2000327 Patent InventorsOOMEN RP 94-A2 WANG J MURDIN AD Chlamydia antigen CPN100708 full length coding WO2000327 sequence. 94-A2 Patent InventorsOOMEN RP WANG J MURDIN AD Chlamydia antigen CPN100708 gene open reading frame. WO2000327 Patent InventorsOOMEN RP 94-A2 WANG J MURDIN AD C. pneumoniae ATP/ADP translocase coding sequence. WO2000391 Patent InventorsDUNN P 57-A1 OOMEN RP WANG J MURDIN AD Chlamydia pneumoniae 98 kDa outer membrane protein WO2000327 CPN100640 gene. 84-A1 Patent InventorsDUNN P OOMEN RP WANG J MURDIN AD Chlamydia pneumoniae 98 kDa outer membrane protein WO2000327 coding region. 84-A1 Patent InventorsDUNN P OOMEN RP WANG J MURDIN AD DNA encoding a 9 kDa cysteine-rich membrane protein. WO2000537 Patent InventorsDUNN P 64-A1 OOMEN RP WANG J MURDIN AD DNA encoding a 60 kDa cysteine-rich membrane protein. WO2000553 Patent InventorsDUNN P 26-A1 OOMEN RP WANG J MURDIN AD A 9 kDa cysteine-rich membrane protein. WO2000537 Patent InventorsDUNN P 64-A1 OOMEN RP WANG J MURDIN AD A 60 kDa cysteine-rich membrane protein of Chlamydia WO2000553 pneumoniae. 26-A1 Patent InventorsDUNN P OOMEN RP WANG J MURDIN AD C. pneumoniae mip (outer membrane protein). WO2000067 Patent InventorsDUNN PL 41-A1 OOMEN RP MURDIN AD C. pneumoniae mip (outer membrane protein) truncated WO2000067 protein. 41-A1 Patent InventorsDUNN PL OOMEN RP MURDIN AD C. pneumoniae omp protein sequence. WO2000067 Patent InventorsDUNN PL 43-A2 OOMEN RP MURDIN AD C. pneumoniae omp protein truncated sequence. WO2000067 Patent InventorsDUNN PL 43-A2 OOMEN RP MURDIN AD Amino acid sequence of the CPN100111 polypeptide. WO2000111 Patent InventorsOOMEN RP 83-A2 MURDIN AD Amino acid sequence of the CPN100224 polypeptide. WO2000111 Patent InventorsOOMEN RP 83-A2 MURDIN AD Amino acid sequence of the CPN100230 polypeptide. WO2000111 Patent InventorsOOMEN RP 83-A2 MURDIN AD Amino acid sequence of the CPN100231 polypeptide. WO2000111 Patent InventorsOOMEN RP 83-A2 MURDIN AD Amino acid sequence of the CPN100232 polypeptide. WO2000111 Patent InventorsOOMEN RP 83-A2 MURDIN AD Amino acid sequence of the CPN100233 polypeptide. WO2000111 Patent InventorsOOMEN RP 83-A2 MURDIN AD Amino acid sequence of the CPN100394 polypeptide. WO2000111 Patent InventorsOOMEN RP 83-A2 MURDIN AD Amino acid sequence of the CPN100395 polypeptide. WO2000111 Patent InventorsOOMEN RP 83-A2 MURDIN AD Amino acid sequence of the POMP91A protein of WO2000111 Chlamydia pneumoniae. 80-A1 Patent InventorsDUNN PL OOMEN RP MURDIN AD Chlamydia pneumoniae antigen CPN100202 protein WO2000067 sequence. 39-A2 Patent InventorsOOMEN RP MURDIN AD Chlamydia pneumoniae antigen CPN100149 protein WO2000067 SEQ ID NO: 2. 40-A1 Patent InventorsOOMEN RP MURDIN AD Chlamydia pneumoniae antigen CPN100605 protein WO2000067 SEQ ID NO: 2. 42-A2 Patent InventorsOOMEN RP MURDIN AD Chlamydia antigen CPN100634. WO2000327 Patent InventorsOOMEN RP 94-A2 WANG J MURDIN AD Chlamydia antigen CPN100635. WO2000327 Patent InventorsOOMEN RP 94-A2 WANG J MURDIN AD Mature Chlamydia antigen CPN100635. WO2000327 Patent InventorsOOMEN RP 94-A2 WANG J MURDIN AD Chlamydia antigen CPN100638. WO2000327 Patent InventorsOOMEN RP 94-A2 WANG J MURDIN AD Chlamydia antigen CPN100639. WO2000327 Patent InventorsOOMEN RP 94-A2 WANG J MURDIN AD Chlamydia antigen CPN100708. WO2000327 Patent InventorsOOMEN RP 94-A2 WANG J MURDIN AD C. pneumoniae ATP/ADP translocase protein sequence. WO2000391 Patent InventorsDUNN P 57-A1 OOMEN RP WANG J MURDIN AD Chlamydia pneumoniae 98 kD putative outer membrane WO2000262 protein. 37-A2 Patent InventorsDUNN PL OOMEN RP MURDIN AD Chlamydia POMP91B precursor protein. WO2000262 Patent InventorsDUNN PL 39-A2 OOMEN RP MURDIN AD Chlamydia pneumoniae 98 kDa outer membrane protein WO2000327 CPN100640. 84-A1 Patent InventorsDUNN P OOMEN RP WANG J MURDIN AD Chlamydia pneumoniae processed 98 kDa outer membrane WO2000327 protein CPN100640. 84-A1 Patent InventorsDUNN P OOMEN RP WANG J MURDIN AD C. pneumoniae mip (outer membrane protein) encoding WO2000067 DNA. 41-A1 Patent InventorsDUNN PL OOMEN RP MURDIN AD C. pneumoniae omp protein encoding DNA. WO2000067 Patent InventorsDUNN PL 43-A2 OOMEN RP MURDIN AD DNA encoding the CPN100111 polypeptide. WO2000111 Patent InventorsOOMEN RP 83-A2 MURDIN AD DNA encoding the CPN100224 polypeptide. WO2000111 Patent InventorsOOMEN RP 83-A2 MURDIN AD DNA encoding the CPN100230 polypeptide. WO2000111 Patent InventorsOOMEN RP 83-A2 MURDIN AD DNA encoding the CPN100231 polypeptide. WO2000111 Patent InventorsOOMEN RP 83-A2 MURDIN AD DNA encoding the CPN100232 polypeptide. WO2000111 Patent InventorsOOMEN RP 83-A2 MURDIN AD DNA encoding the CPN100233 polypeptide. WO2000111 Patent InventorsOOMEN RP 83-A2 MURDIN AD DNA encoding the CPN100394 polypeptide. WO2000111 Patent InventorsOOMEN RP 83-A2 MURDIN AD DNA encoding the CPN100395 polypeptide. WO2000111 Patent InventorsOOMEN RP 83-A2 MURDIN AD Nucleotide sequence of the POMP91A gene of Chlamydia WO2000111 pneumoniae. 80-A1 Patent InventorsDUNN PL OOMEN RP MURDIN AD Chlamydia pneumoniae antigen CPN100202 nucleotide WO2000067 sequence. 39-A2 Patent InventorsOOMEN RP MURDIN AD Chlamydia pneumoniae antigen CPN100149 protein WO2000067 encoding DNA SEQ ID NO: 1. 40-A1 Patent InventorsOOMEN RP MURDIN AD Chlamydia pneumoniae antigen CPN100605 protein WO2000067 encoding DNA SEQ ID NO: 1. 42-A2 Patent InventorsOOMEN RP MURDIN AD

[0071] When such blebs are present in a vaccine formulation they may be more protective against Chlamydia pneumoniae infection than the use of the protein/antigen in isolation.

[0072] Particularly beneficial pairs of Chlamydia pneumoniae antigens have also been found. Thus in a further aspect a Gram-negative bleb (preferably from meningococcus) is provided presenting on its surface both the PorB and MOMP outer membrane proteins from Chlamydia pneumoniae. Furthermore, a Gram-negative bleb (preferably from meningococcus) is provided presenting on its surface both MOMP and one or more Pmp outer membrane proteins from Chlamydia pneumoniae. A Gram-negative bleb (preferably from meningococcus) is additionally provided presenting on its surface both the PorB and one or more Pmp outer membrane proteins from Chlamydia pneumoniae. A Gram-negative bleb (preferably from meningococcus) is also provided presenting on its surface both the PorB and Npt1 proteins from Chlamydia pneumoniae. A Gram-negative bleb (preferably from meningococcus) is additionally provided presenting on its surface both the Npt1 and one or more Pmp proteins from Chlamydia pneumoniae. Lastly, a Gram-negative bleb (preferably from meningococcus) is provided presenting on its surface both the Npt1 and MOMP proteins from Chlamydia pneumoniae. Bacterial strains from which these blebs are derived are further aspects of this invention.

[0073] Such prepartions formulated as a vaccine can give enhanced protection to a host against Chlamydia than when a single antigen is administered.

[0074] Preferably the bleb has been derived from a strain which has been modified to upregulate one or more protective outer membrane antigens (see below; for instance for meningocococcal protective outer membrane antigens see section “Neisserial bleb preparations” for those antigens that should preferably be upregulated).

[0075] Preferably the bleb has been derived from a strain which has been modified to downregulate one or more immunodominant variable or non-protective outer membrane antigens (as described below; for instance for meningocococcal variable/non-protective outer membrane antigens see section “Neisserial bleb preparations” for those antigens that should preferably be downregulated).

[0076] Preferably the blebs are derived from a strain which has a detoxified lipid A portion of bacterial LPS, due to the strain having been engineered to reduce or switch off expression of one or more genes which cause LPS to be toxic (preferably selected from the following genes, or homologues thereof htrB, msbB and lpxK; see section below).

[0077] Preferably the blebs are derived from a strain which has a detoxified lipid A portion of bacterial LPS, due to the strain having been engineered to express at a higher level of one or more genes producing a gene product that is capable of detoxifying LPS (preferably selected from the following genes, or homologues thereof: pmrA, pmrB, pmrE and pmrF; see section below).

[0078] Vaccine compositions comprising the bleb of the invention and a pharmaceutically suitable excipient or carrier are also envisaged. Preferably the vaccine additionally comprising a mucosal adjuvant. Mucosal adjuvants are well known in the art (see Vaccine Design “The subunit and adjuvant approach” (eds Powell M. F. & Newman M. J.) (1995) Plenum Press New York). A preferred mucosal adjuvant is LT2 (or LTII, which can be split into LTIIa and LTIIb—see Martin et al. Infection and Immunity, 2000, 68:281-287). Preferably such vaccines should be formulated and administered as described below in “Vaccine formulations”.

[0079] The content of blebs per dose in the vaccine will typically be in the range 1-100 μg, preferably 5-50 μg, most typically in the range 5-25 μg.

[0080] Optimal amounts of components for a particular vaccine can be ascertained by standard studies involving observation of appropriate immune responses in subjects. Following an initial vaccination, subjects may receive one or several booster immunisations adequately spaced.

[0081] The efficacy of a C. pneumoniae vaccine can be evaluated in a mouse model of infection such as the one described by Murdin et al., 2000, J. Infect. Dis. 181 (suppl 3):S5444-5 1. The protection elicited by a vaccine formulation can be assessed by reduction of the bacterial load in the lung after a challenge infection with C. pneumoniae.

[0082] A method of preventing Chlamydia pneumoniae infection in a host is also provided comprising the steps of administering an effective amount of the above vaccine to a host in need thereof. Preferably the vaccine is mucosally administered via either an intranasal, intradermal or oral route.

[0083] Further Improvements in the Bacteria and blebs of the Invention

[0084] The Gram-negative bacterium of the invention may be further genetically engineered by one or more processes selected from the following group: (a) a process of down-regulating expression of immunodominant variable or non-protective antigens, (b) a process of upregulating expression of protective OMP antigens, (c) a process of down-regulating a gene involved in rendering the lipid A portion of LPS toxic, (d) a process of upregulating a gene involved in rendering the lipid A portion of LPS less toxic, and (e) a process of down-regulating synthesis of an antigen which shares a structural similarity with a human structure and may be capable of inducing an auto-immune response in humans. These processes are described in detail in WO 01/09350 (incorporated by reference herein).

[0085] Such bleb vaccines of the invention are designed to focus the immune response on a few protective (preferably conserved) antigens or epitopes—formulated in a multiple component vaccine. Where such antigens are integral OMPs, the outer membrane vesicles of bleb vaccines will ensure their proper folding. This invention provides methods to optimize the OMP and LPS composition of OMV (bleb) vaccines by deleting immunodominant variable as well as non protective OMPs, by creating conserved OMPs by deletion of variable regions, by upregulating expression of protective OMPs, and by eliminating control mechanisms for expression (such as iron restriction) of protective OMPs. In addition the invention provides for the reduction in toxicity of lipid A by modification of the lipid portion or by changing the phosphoryl composition whilst retaining its adjuvant activity or by masking it. Each of these new methods of improvement individually improve the bleb vaccine, however a combination of one or more of these methods work in conjunction so as to produce an optimised engineered bleb vaccine which is immuno-protective and non-toxic—particularly suitable for paediatric use.

[0086] (a) A Process of Down-Regulating Expression of Immunodominant Variable or Non-Protective Antigens

[0087] Many surface antigens are variable among bacterial strains and as a consequence are protective only against a limited set of closely related strains. An aspect of this invention covers the reduction in expression, or, preferably, the deletion of the gene(s) encoding variable surface protein(s) which results in a bacterial strain producing blebs which, when administered in a vaccine, have a stronger potential for cross-reactivity against various strains due to a higher influence exerted by conserved proteins (retained on the outer membranes) on the vaccinee's immune system. Examples of such variable antigens include: for Neisseria—pili (PilC) which undergoes antigenic variations, PorA, Opa, TbpB, FrpB; for H. influenzae—P2, P5, pilin, IgA1-protease; and for Moraxella—CopB, OMP106.

[0088] Other types of gene that could be down-regulated or switched off are genes which, in vivo, can easily be switched on (expressed) or off by the bacterium. As outer membrane proteins encoded by such genes are not always present on the bacteria, the presence of such proteins in the bleb preparations can also be detrimental to the effectiveness of the vaccine for the reasons stated above. A preferred example to down-regulate or delete is Neisseria Opc protein. Anti-Opc immunity induced by an Opc containing bleb vaccine would only have limited protective capacity as the infecting organism could easily become Opc⁻ . H. influenzae HgpA and HgpB are other examples of such proteins.

[0089] In process a), these variable or non-protective genes are down-regulated in expression, or terminally switched off. This has the surprising advantage of concentrating the immune system on better antigens that are present in low amounts on the outer surface of blebs.

[0090] The strain can be engineered in this way by a number of strategies including transposon insertion to disrupt the coding region or promoter region of the gene, or point mutations or deletions to achieve a similar result. Homologous recombination may also be used to delete a gene from a chromosome (where sequence X comprises part (preferably all) of the coding sequence of the gene of interest). It may additionally be used to change its strong promoter for a weaker (or no) promoter. All these techniques are described in WO 01/09350 (published by WIPO on Aug. 2, 2001 and incorporated by reference herein).

[0091] (b) A Process of Upregulating Expression of Protective OMP Antigens

[0092] This may be done by inserting a copy of such a protective OMP into the genome (preferably by homologous recombination), or by upregulating expression of the native gene by replacing the native promoter for a stronger promoter, or inserting a strong promoter upstream of the gene in question (also by homologous recombination). Such methods can be accomplished using the techniques described in WO 01/09350 (published by WIPO on Aug. 2, 2001 and incorporated by reference herein).

[0093] Such methods are particularly useful for enhancing the production of immunologically relevant Bleb components such as outer-membrane proteins and lipoproteins (preferably conserved OMPs, usually present in blebs at low concentrations).

[0094] (c) A Process of Down-Regulating a Gene Involved in Rendering the Lipid A Portion of LPS Toxic

[0095] The toxicity of bleb vaccines presents one of the largest problems in the use of blebs in vaccines. A further aspect of the invention relates to methods of genetically detoxifying the LPS present in Blebs. Lipid A is the primary component of LPS responsible for cell activation. Many mutations in genes involved in this pathway lead to essential phenotypes. However, mutations in the genes responsible for the terminal modifications steps lead to temperature-sensitive (htrB) or permissive (msbB) phenotypes. Mutations resulting in a decreased (or no) expression of these genes result in altered toxic activity of lipid A. Indeed, the non-lauroylated (htrB mutant) [also defined by the resulting LPS lacking both secondary acyl chains] or non-myristoylated (msbB mutant) [also defined by the resulting LPS lacking only a single secondary acyl chain] lipid A are less toxic than the wild-type lipid A. Mutations in the lipid A 4′-kinase encoding gene (lpxK) also decreases the toxic activity of lipid A.

[0096] Process c) thus involves either the deletion of part (or preferably all) of one or more of the above open reading frames or promoters. Alternatively, the promoters could be replaced with weaker promoters. Preferably the homologous recombination techniques are used to carry out the process. Preferably the methods described in WO 01/09350 (published by WIPO on Aug. 2, 2001 and incorporated by reference herein) are used. The sequences of the htrB and msbB genes from Neisseria meningitidis B, Moraxella catarrhalis, and Haemophilus influenzae are provided in WO 01/09350 for this purpose.

[0097] (d) A Process of Upregulating a Gene Involved in Rendering the Lipid A Portion of LPS less Toxic

[0098] LPS toxic activity could also be altered by introducing mutations in genes/loci involved in polymyxin B resistance (such resistance has been correlated with addition of arninoarabinose on the 4′ phosphate of lipid A). These genes/loci could be pmrE that encodes a UDP-glucose dehydrogenase, or a region of antimicrobial peptide-resistance genes common to many enterobacteriaciae which could be involved in aminoarabinose synthesis and transfer. The gene pmrF that is present in this region encodes a dolicol-phosphate manosyl transferase (Gunn J. S., Kheng, B. L., Krueger J., Kim K., Guo L., Hackett M., Miller S. I. 1998. Mol. Microbiol. 27: 1171-1182).

[0099] Mutations in the PhoP-PhoQ regulatory system, which is a phospho-relay two component regulatory system (f.i. PhoP constitutive phenotype, PhoP^(c)), or low Mg⁺⁺ environmental or culture conditions (that activate the PhoP-PhoQ regulatory system) lead to the addition of aminoarabinose on the 4′-phosphate and 2-hydroxymyristate replacing myristate (hydroxylation of myristate). This modified lipid A displays reduced ability to stimulate E-selectin expression by human endothelial cells and TNF-α secretion from human monocytes.

[0100] Process d) involves the upregulation of these genes using a strategy as described in WO 01/09350 (published by WIPO on Aug. 2, 2001 and incorporated by reference herein).

[0101] (e) A Process of Down-Regulating Synthesis of an Antigen which Shares a Structural Similarity with a Human Structure and may be Capable of Inducing an Auto-Immune Response in Humans

[0102] The isolation of bacterial outer-membrane blebs from encapsulated Gram-negative bacteria often results in the co-purification of capsular polysaccharide. In some cases, this “contaminant” material may prove useful since polysaccharide may enhance the immune response conferred by other bleb components. In other cases however, the presence of contaminating polysaccharide material in bacterial bleb preparations may prove detrimental to the use of the blebs in a vaccine. For instance, it has been shown at least in the case of N. meningitidis that the serogroup B capsular polysaccharide does not confer protective immunity and is susceptible to induce an adverse auto-immune response in humans. Consequently, process e) of the invention is the engineering of the bacterial strain for bleb production such that it is free of capsular polysaccharide. The blebs will then be suitable for use in humans. A particularly preferred example of such a bleb preparation is one from N. meningitidis serogroup B devoid of capsular polysaccharide.

[0103] This may be achieved by using modified bleb production strains in which the genes necessary for capsular biosynthesis and/or export have been impaired as described in WO 01/09350 (published by WIPO on Aug. 2, 2001 and incorporated by reference herein). A preferred method is the deletion of some or all of the Neisseria meningitidis cps genes required for polysaccharide biosynthesis and export. For this purpose, the replacement plasmid pMF121 (described in Frosh et al. 1990, Mol. Microbiol. 4:1215-1218) can be used to deliver a mutation deleting the cpsCAD (+galE) gene cluster. Alternatively the siaD gene could be deleted, or down-regulated in expression (the meningococcal siaD gene encodes alpha-2,3-sialyltransferase, an enzyme required for capsular polysaccharide and LOS synthesis). Such mutations may also remove host-similar structures on the saccharide portion of the LPS of the bacteria.

[0104] Combinations of Methods a)-e)

[0105] It may be appreciated that one or more of the above processes may be used to produce a modified strain from which to make improved bleb preparations of the invention. Preferably one such process is used, more preferably two or more (2, 3, 4, or 5) of the processes are used in order to manufacture the bleb vaccine. As each additional method is used in the manufacture of the bleb vaccine, each improvement works in conjunction with the other methods used in order to make an optimised engineered bleb preparation.

[0106] A preferred meningococcal (particularly N. meningitidis B) bleb preparation comprises the use of processes b), c) and e) (optionally combined with process a)). Such bleb preparations are safe (no structures similar to host structures), non-toxic, and structured such that the host immune response will be focused on high levels of protective (and preferably conserved) antigens. All the above elements work together in order to provide an optimised bleb vaccine.

[0107] Similarly for M. catarrhalis, non-typeable H. influenzae, gonococcus, and non serotype B meningococcal strains (e.g. serotype A, C, Y or W), preferred bleb preparations comprise the use of processes b) and c), optionally combined with process a).

[0108] Preferred Neisserial bleb Preparations

[0109] One or more of the following genes (encoding protective antigens) are preferred for upregulation via process b) when carried out on a Neisserial strain, including gonococcus, and meningococcus (particularly N. meningitidis B): NspA (WO 96/29412), Hsf-like (WO 99/31132), Hap (PCT/EP99/02766), PorA, PorB, OMP85 (WO 00/23595), PilQ (PCT/EP99/03603), PldA (PCT/EP99/06718), FrpB (WO 96/31618), TbpA (U.S. Pat. No. 5,912,336), TbpB, FrpA/FrpC (WO 92/01460), LbpA/LbpB (PCT/EP98/05117), FhaB (WO 98/02547), HasR (PCT/EP99/05989), lipo02 (PCT/EP99/08315), Thp2 (WO 99/57280), MltA (WO 99/57280), and ctrA (PCT/EP00/00135). They are also preferred as genes which may be heterologously introduced into other Gram-negative bacteria.

[0110] One or more of the following genes are preferred for downregulation via process a): PorA, PorB, PilC, ThpA, TbpB, LbpA, LbpB, Opa, and Opc (most preferably PorA).

[0111] One or more of the following genes are preferred for downregulation via process c): htrB, msbB and lpxK (most preferably msbB which removes only a single secondary acyl chain from the LPS molecule).

[0112] One or more of the following genes are preferred for upregulation via process d): pmrA, pmrB, pmrE, and pmrF.

[0113] One or more of the following genes are preferred for downregulation via process e): galE, siaA, siaB, siaC, siaD, ctrA, ctrB, ctrC, and ctrD (the genes are described in described in WO 01/09350—published by WIPO on Aug. 2, 2001 and incorporated by reference herein).

[0114] Many of the above open reading frames and upstream regions are described in WO 01/09350 (incorporated by reference herein).

[0115] Preferred gonococcal genes to upregulate via process b) include one or more of the following: [SEQ ID NO:79] Neisseria gonorrheae lactoferrin receptor precursor (lbpA) gene, complete cds. ACCESSION U16260 VERSION  U16260.1 GI:915277 Source: Neisseria gonorrhoeae/strain=“FA19” gene=“lbpA” nucleotides: 278..3109 protein_id=“AAC13780.1”/db_xref=“GI:915278”             /translation= “MNKKHGFPLTLTALAIATAFPAYAAQAGAAALDAAQSQSLKEVT VPAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW EGLLIRTERQGRETRPHGDIADGVEYGIDRLDAFRQTYDIKRKTTEPFFLVEGENTLK PVAKLAGYGIYLNRQLNRWVKERIEQNQPLSAEEEAQVREAQARHENLSAQAYTGGGR ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR DKSGVYDGDDFRDGLYFVPNIEEWKGDKNLVKGIGLKYSRTKFIDEHHRRRRMGLLYR YENEKYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAVSRPEQLSHNAARISESTG FDEKNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD FSLGGRYDRKNFTTSEELVRSGRYADRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQE LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADQ KTKLPDSAGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYGKLPEGLYTTLAYNRI KPKSVSNRPDLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY LAGDQKRYSAGRVTSSWKTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES                      TANRHGGDSNYGRYAAPGRNFSLALEMKF” [SEQ ID NO:80]    1 ctcgggataa cggcatcaat ctttcgggaa atggttcgac taatcctcaa agtttcaaag   61 ccgacaatct tcttgtaacg ggcggctttt acggcccgca ggcggcggaa ttgggcggca  121 ctattttcaa taaggatggg aaatctcttg gtataactga agatattgaa aatgaagttg  181 aaaatgaagc tgatgttggc gaacagttag aacctgaagt taaaccccaa ttcggcgtgg  241 tattcggtgc gaagaaagat aataaagagg tggaaaaatg aataagaaac acggttttcc  301 gctgactttg acggcgttgg ccattgcaac cgcttttccg gcttatgctg cccaagcggg  361 ggcggcggca cttgatgcgg cgcaaagtca atcattgaaa gaggttaccg tccgtgccgc  421 caaagtggga cggcgatcga aagaggcgac aggtttgggc aaaatcgtca aaacgtcgga  481 aacgttgaac aaagaacagg tactcggtat ccgcgacctg acgcgctacg atccgggcgt  541 ggcggttgtc gaacagggca acggcgcgag cggcggctac tcgatacgcg gcgtagataa  601 aaaccgtgtg gcggtttcgg ttgacggcgt tgcccaaata caggcgttta ccgtgcaggg  661 atcgttgagc ggatacggcg gacgcggcgg cagcggcgca atcaacgaaa tcgaatatga  721 aaacatcagc acggtggaaa tcgacaaagg cgccggttcg tccgatcacg gcagcggcgc  781 actcggcggc gcggtcgcct tccgcaccaa agaggcggca gacctgattt cagacggcaa  841 aagctggggg atacaggcaa aaaccgctta cggcagtaaa aaccgccaat ttatgaagtc  901 gctcggcgcg gggttcagca aagacggttg ggaagggctg ctaatccgaa ccgaacgcca  961 agggcgggaa acgcgcccgc acggcgatat tgcggacggg gtggaatacg gcatagaccg 1021 tttggacgcg ttccgccaga catacgatat taaacgcaag acaacagagc catttttctt 1081 agtagagggc gagaatacac tcaagcccgt ggcaaaattg gcgggctacg ggatatattt 1141 gaaccgccag ctcaaccgct gggtaaaaga acgtattgaa caaaatcagc ctttaagtgc 1201 tgaagaagag gcgcaggtgc gggaggcgca ggcgcgccac gaaaacctgt ccgcccaagc 1261 ctacacgggc ggcggcagga tattgcccga tccgatggat taccgcagcg gctcttggct 1321 tgccaagctg ggctaccgct tcggcggcag gcattatgtc ggcggcgtgt ttgaggatac 1381 caaacagcgt tacgacatcc gcgatatgac ggaaaaacag tattacggta cggacgaggc 1441 ggaaaagttt agagacaaga gcggggtgta cgacggcgac gatttccgcg acggcttgta 1501 ttttgtgccg aatatagaag agtggaaggg cgataaaaat ttggtcaagg gcataggttt 1561 gaaatattcc cgcaccaaat ttattgacga acatcaccgc cgccgccgta tgggtttgct 1621 gtaccgttat gaaaatgaga aatactcgga caactgggcg gataaggcgg tgttgtcgtt 1681 tgacaaacag ggcgtggcaa ccgacaacaa cacgctgaag ctgaattgcg ccgtgtatcc 1741 tgccgtggac aaatcctgcc gcgcgtcggc ggacaaaccg tattcctacg acagcagcga 1801 ccgtttccac taccgcgaac agcacaatgt tttgaatgcc tcgtttgaga agtcgctgaa 1861 aaacaaatgg acgaaacacc atctgacttt gggcttcggt tacgatgctt ccaaagcagt 1921 atcccgccca gaacagcttt cccacaatgc ggcaaggatt tcggaatcca cgggattcga 1981 tgaaaagaat caagataagt accgtttggg taagcccgaa gtcgtcgaag ggtcggtctg 2041 cggctatatc gaaaccctgc gttcccgcaa atgcgtgcca agaaaaatca acggcagcaa 2101 tatccacatt tctttgaacg accgtttttc aatcggcaaa tattttgatt tcagcttggg 2161 cggcaggtac gaccggaaaa acttcaccac gtcggaagaa ctcgtccgca gcgggcggta 2221 tgccgaccgt tcgtggaaca gcggcatcgt gttcaaaccg aaccggcatt tttccgtgtc 2281 ttaccgcgcc tccagcggct tcagaacgcc ttccttccaa gaacttttcg ggatagacat 2341 ttatcacgat tatccgaaag gctggcagcg tcccgccctg aaatcggaaa aggcagccaa 2401 ccgggaaatc ggtttgcagt ggaagggcga tttcggcttt ttggaaatca gcagtttccg 2461 caaccgttat accgatatga ttgccgttgc cgatcaaaaa accaaattgc cggattcagc 2521 aggacgattg acagagattg atatacgcga ttattacaat gcccaaaata tgtcgcttca 2581 aggcatcaac atcttgggga aaatcgactg gaacggcgta tacggcaaac tgcccgaagg 2641 cctgtacacc acattggcgt acaaccgtat caaaccgaaa tcggtatcca accggccgga 2701 cttgtccctc cgcagctatg ctttggatgc ggtacagccg tcgcgttatg ttttggggtt 2761 cggatacgac cagcccgagg ggaaatgggg cgcaaacatt atgctgacct attccaaagg 2821 gaaaaaccct gacgagcttg cttatctggc aggcgatcaa aaacgatatt cggcaggaag 2881 ggttacgtct tcttggaaaa cggcagatgt ttccgcttat ctgaatctga aaaaacggct 2941 gaccttgagg gcggctatct acaatatcgg caactaccgc tacgttactt gggaatcctt 3001 gcgccagact gcggaaagca cggcaaaccg gcacggcggc gacagcaact atggaaggta 3061 tgccgcaccg ggcaggaact tcagcctcgc gctcgaaatg aagttttaaa ggaaatgccg 3121 tctggaagct tgatctgcac cccaaaagtc ggactaaacc gccaactgat taaggtgcag 3181 gtttttttga ttcaatataa acaagatttc cgccgtcatt cccgcgcagg cgggaatccg 3241 gacattcaat gctaaggcaa tttatcggaa atgactgaaa ctcaaaaaac cggattccca // [SEQ ID NO:81] Neisseria gonorrhoeae lactoferrin binding protein B precursor. Source: Neisseria gonorrhoeae”/strain=“FA19” ACCESSION   AAD08809 PID         g4106393 VERSION     AAD08809.1  GI:4106393 /gene=“lbpB”   coding sequence: 1..728, “AF072890.1:310..2496”    1 mrklnyygia llplmlascg gnfgvqpvve stptaypvtf kskdvptspp paepsvettp   61 vnrpavgaam rllrrntafh redgtaipds kqaeeklsfk egdvlflygs kgnklqqlks  121 eihkrdsdve irtsekenkk ygyefvdagy vytkngkdei eqnsggkrft hrfgydgfvy  181 ysgerpsqsl psagtvkyfg nwqymtdakr hrtgkavasd dlgyitfygn digatsyaak  241 daddrekhpa eytvdfdkki lkgeliknqy vqkkndpkkp ltiynitadl ngnrftgsak  301 vntevktrha dkeylffhtd adqrleggff gdngeelagr fisndngvfg vfagkqktna  361 sgtnpampfg khtkildslk isvdeatden prpfevstmp dfghpdkllv egreiplvsk  421 ektidladgr kmtvsaccdf ltyvklgrik terpavkpka qdeedsginn geesedeeei  481 aeesedevse ddngededei veeeadeaee ieeeaeeeep eeespeegng vsdgippape  541 alkgrdidlf lkgirtaead ipktgtahyt gtwearigep iqwdnkadka akaefdvdfg  601 nksisgtlte qngvepafri engviegngf hptartrdng inlsgngstn pqsfkadnll  661 vtggfygpqa aelggtifnk dgkslgited ienevenead vgeqlepevk pqfgvvfgak  721 kdnkevek [SEQ ID NO:82] Neisseria gonorrhoeae transferrin-binding protein A (tbpA) gene, complete cds. ACCESSION   AF241227 VERSION     AF241227.1  GI:9719361 source          :Neisseria gonorrhoeae/strain=“Pgh3-2” gene “tbpA” coding sequence: 223..2946 /protein_id=“AAF97766.1” /db_xref=“GI:9719362” /translation= “MQQQHLFRFNILCLSLMTALPAYAENVQAGQAQEKQLDTIQVKA KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA LLIRTGRHAGEIRAHEAAGRGVQSFNRLAPVEDGSDYAYFVVEGECPDGYAACKDKPK KDVVGEDKRQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERTQ QTFDTRDMTVPAFLTKAVFDANSKQAGSLRGNGKYAGNHKYGGLFTNGENNAPVGAEY GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS DKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGYDRFGSN LRHQDYYYQSANRAYSLKTPPQNNGKKTSPYWVSIGRGNVVTGQICRSGNNTYTDCTP RSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLSWNTGIVL KPADWLDLTYRTSTGFRLPSFAEMYGWRSGDKIKAVKIDPEKSFNKEAGIVFKGDFGN LEASWFDNAYRDLIVRGYEAEIKNGKEQAKGAPAYLNAQSARITGINILGKIDWNGVW DKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGSGYDQPEGKWGV NGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTVKKHFTLRA                      GVYNLLNHRYVTWENVRQTAAGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF” [SEQ ID NO:83]    1 cgaagagttg ggcggatggt ttgcctatcc gggcaatgaa caaacgaaaa atgcgcaagc   61 ttcatccggc aatggaaatt cagcaggcag cgcgaccgtg gtattcggtg cgaaacgcca  121 aaagcttgtg caataagcac ggctgccgaa caatcgagaa taaggcttca gacggcatcg  181 ttcctgccga ttccgtctga aagcgaagat tagggaaaca ctatgcaaca gcaacatttg  241 ttccgattca atattttatg cctgtcttta atgactgcgc tgcccgctta tgcagaaaat  301 gtgcaagccg gacaagcaca ggaaaaacag ttggacacca tacaggtaaa agccaaaaaa  361 cagaaaaccc gccgcgataa cgaagtaacc ggtttgggca aattggtcaa aaccgccgac  421 acactcagca aagaacaggt actcgacatc cgcgacctga cgcgttacga ccccggcatc  481 gccgtcgtcg aacaggggcg cggcgcaagc tcgggctact cgatacgcgg tatggacaaa  541 aaccgcgtct ccttgacggt ggacggcttg gcgcaaatac agtcctacac cgcgcaggcg  601 gcattgggcg ggacgaggac ggcgggcagc agcggcgcaa tcaatgaaat cgagtatgaa  661 aacgttaagg ctgtcgaaat cagcaaaggc tcaaactcgg tcgaacaagg cagcggcgca  721 ttggcgggtt cggtcgcatt tcaaaccaaa accgcagacg atgttatcgg ggaaggcagg  781 cagtggggca ttcagagtaa aaccgcctat tccggcaaaa accgggggct tacccaatcc  841 atcgcgctgg cggggcgcat cggcggtgcg gaggctttgc tgatccgcac cggccggcac  901 gcgggggaaa tccgcgccca cgaagccgcc ggacgcggcg ttcagagctt taacaggctg  961 gcgccggttg aagacggcag tgactatgcc tattttgtgg tcgaaggaga atgccctgat 1021 ggatatgcgg cttgtaaaga caaaccgaaa aaagatgttg tcggcgaaga caaacgtcaa 1081 acggtttcca cccgagacta cacgggcccc aaccgcttcc ttgccgatcc gctttcatac 1141 gaaagccggt cgtggctgtt ccgcccgggt tttcgttttg agaataagcg gcactacatc 1201 ggcggcatac tcgaacgcac gcaacaaact ttcgacacgc gcgatatgac ggttccggca 1261 ttcctgacca aggcggtttt tgatgcaaat tcaaaacagg cgggttcttt gcgcggcaac 1321 ggcaaatacg cgggcaacca caaatacggc gggctgttta ccaacggcga aaacaatgcg 1381 ccggtgggcg cggaatacgg tacgggcgtg ttttacgacg agacgcacac caaaagccgc 1441 tacggtttgg aatatgtcta taccaatgcc gataaagaca cttgggcgga ttatgcccgc 1501 ctctcttacg accggcaggg catcggtttg gacaaccatt ttcagcagac gcactgttct 1561 gccgacggtt cggacaaata ttgccgcccg agtgccgaca agccgttttc ctattacaaa 1621 tccgaccgcg tgatttacgg ggaaagccac aggctcttgc aggcggcatt caaaaaatcc 1681 ttcgataccg ccaaaatccg ccacaacctg agcgtgaatc tcggttacga ccgcttcggc 1741 tctaatctgc gccatcagga ttattattat caaagtgcca accgcgccta ttcgttgaaa 1801 acgccccctc aaaacaacgg caaaaaaacc agcccctatt gggtcagcat aggcagggga 1861 aatgtcgtta cggggcaaat ctgccgctcg ggcaacaata cttatacgga ctgcacgccg 1921 cgcagcatca acggcaaaag ctattacgcg gcggtccggg acaatgtccg tttgggcagg 1981 tgggcggatg tcggcgcggg cttgcgctac gactaccgca gcacgcattc ggacgacggc 2041 agcgtttcca ccggcacgca ccgcaccctg tcctggaaca ccggcatcgt cctcaaacct 2101 gccgactggc tggatttgac ttaccgcact tcaaccggct tccgcctgcc ctcgtttgcg 2161 gaaatgtacg gctggcggtc gggcgataaa ataaaagccg tcaaaatcga tccggaaaaa 2221 tcgttcaaca aagaagccgg catcgtgttt aaaggcgatt tcggcaactt ggaggcaagt 2281 tggttcgaca atgcctaccg cgatttgatt gtccggggtt atgaagcgga aattaaaaac 2341 ggcaaagaac aagccaaagg cgccccggct tacctcaatg cccaaagcgc gcggattacc 2401 ggcatcaata ttttgggcaa aatcgattgg aacggcgtat gggataaatt gcccgaaggt 2461 tggtattcta catttgccta taatcgtgtc cgtgtccgcg acatcaaaaa acgcgcagac 2521 cgcaccgata ttcaatcaca cctgtttgat gccatccaac cctcgcgcta tgtcgtcggc 2581 tcgggctatg accaaccgga aggcaaatgg ggcgtgaacg gtatgctgac ttattccaaa 2641 gccaaggaaa tcacagagtt gttgggcagc cgggctttgc tcaacggcaa cagccgcaat 2701 acaaaagcca ccgcgcgccg tacccgccct tggtatattg tggatgtgtc cggttattac 2761 acggttaaaa aacacttcac cctccgtgcg ggcgtgtaca acctcctcaa ccaccgctat 2821 gttacttggg aaaatgtgcg gcaaactgcc gccggcgcag tcaaccaaca caaaaatgtc 2881 ggcgtttaca accgatatgc cgcccccggc cgcaactaca catttagctt ggaaatgaag 2941 ttctaaacgt ccgaacgccg caaatgccgt ctgaaaggct tcagacggcg ttttttacac 3001 aatccccacc gtttcccatc cttcccgata caccg [SEQ ID NO:84] Neisseria gonorrhoeae strain UU1008 transferrin-binding protein 2 (tbpB) gene, complete cds. ACCESSION   U65222 VERSION     U65222.1  GI:2286066 Source: Neisseria gonorrhoeae/strain=“UU1008” gene=“tbpB” coding sequence: 1..2052 /protein_id=“AAB64243.1” /db_xref=“GI:2286067” /translation= “MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRAAPKY QDVPSKKPEARKDQGGYGFAMRFKRRNWYRAANENEVKLKESDWEQTDDDEIKNPFKQ KNIINALPGNEGELLQDSSQQGKGTSKVRDHHDFKYVWSGFFYKRIKITTKKDESHKI IEARSGPDGYIFYKGRNPSRKLPVSGEVTYKGTWDFLTDVKANQKFTDLGNASTKSGD QYSAFSGELDYIVKKEEDKKEKHKGLGLTTEITVDFEKKTLIGKLIKNNMLINNNTKP TTQYYSLEAQVTGNRFSGKAMATEKGENKQHPFVSDSSSLSGGFFGPQGEELGFRFLS DDGKVAVVGSAKTKDETASSGGTSGGASVSTSNGAAGTSSENKLTTVLDAVELTPNGK KIKDLDNFSNAAQLVVDGIMIPLLPKDSESGGSHTDKGENGKTAFIYETTYTPESDKE DAQTGMATNGVQTVSNTAGGTSGKTKTHYEVQACCSNLNYLKYGLLTRKNSSQADAKM GQVEQSMFLQGERTDEKEIPQEQNVVYSGTWYGHIATNGTSWTREASDQENGNRANFD VNFKDKRITGTLTAENRSEATFTIEAMIEGNGFKGTAKTGNGGFAPDQNSSTGTHKVH ITNAAVQGGFYGPNAEELGGWFAYPGNGQTKNAQTSSGNGNSAGSATVVFGAKRQQLV                      K” [SEQ ID NO:85]    1 atgaacaatc cattggtgaa tcaggctgct atggtgctgc ccgtgttttt gttgagcgct   61 tgtctgggcg gaggcggcag tttcgatctt gattctgtcg ataccgaagc cccgcgtgcc  121 gcgccaaagt atcaagatgt tccttccaaa aaaccggaag cccgaaaaga ccaaggcgga  181 tacggtttcg cgatgcgctt caagcggcgg aattggtatc gggcggcaaa cgaaaacgag  241 gttaaactga aagagagtga ttgggaacaa acggatgatg atgagatcaa aaaccctttc  301 aaacaaaaaa atattattaa tgccttacct ggaaatgagg gggaattatt gcaagattcc  361 agtcaacaag gtaagggtac atctaaggtt agggaccatc acgattttaa atacgtatgg  421 tcgggttttt tttataaacg gattaagatt acaactaaaa aagacgaatc tcataaaata  481 atcgaagcca gaagcggtcc tgacggttat attttttata aaggcagaaa tccctcgaga  541 aaacttcctg tttcagggga ggttacgtac aaaggtactt gggatttttt aactgatgtg  601 aaagcaaatc agaaatttac agatttagga aatgcttcta cgaaatccgg agaccaatat  661 agtgcttttt ccggggagtt ggattatata gtcaaaaaag aggaggataa aaaagaaaag  721 cacaaaggtt tgggattaac aacggaaata acggttgatt ttgagaaaaa aaccctgatc  781 ggaaaattaa ttaaaaacaa catgttaatc aataataaca ctaaacccac cacccaatat  841 tacagccttg aggctcaagt aacaggcaac cgcttcagcg gcaaggcgat ggcaaccgaa  901 aaaggcgaaa acaaacaaca tccctttgtt tccgactcgt cttctctgag cggcggcttt  961 ttcggcccgc agggtgagga attgggtttc cgctttttga gcgacgatgg aaaagttgcc 1021 gttgtcggca gcgcgaaaac caaagacgaa accgcaagca gtggcggcac ttcgggcggt 1081 gcaagcgttt ccacatcaaa cggtgcggca ggcacgtcgt ctgaaaacaa gctgaccacg 1141 gttttggatg cggttgaatt gacaccaaac ggcaagaaaa tcaaagatct cgacaacttc 1201 agcaacgccg cccaactggt tgtcgacggc attatgattc cgctcctgcc caaggattcc 1261 gaaagcgggg gcagtcatac agataaaggt gaaaacggca aaacagcctt tatctacgaa 1321 acaacctaca cgccggaaag tgataaagaa gacgctcaaa caggtatggc gaccaatggc 1381 gtgcaaaccg tttcaaatac ggcaggcggc acaagtggca aaacaaaaac ccattatgaa 1441 gtccaagcct gctgttccaa cctcaattat ctgaaatacg ggttgctgac gcgtaaaaac 1501 agtagtcaag ctgacgctaa aatgggacaa gttgaacaaa gtatgttcct ccaaggcgag 1561 cgcaccgatg aaaaagaaat tccacaagaa caaaatgtcg tttattcagg cacttggtac 1621 gggcatattg ccaccaacgg cacaagttgg acccgcgaag cctccgatca ggaaaatggt 1681 aatcgggcaa attttgacgt gaatttcaaa gacaaaagaa ttaccggcac gttaaccgct 1741 gaaaacaggt cggaggcaac ctttaccatt gaagccatga ttgagggcaa cggctttaaa 1801 ggtacggcga aaaccggtaa tggcggcttt gcgccggatc aaaacagcag caccggtaca 1861 cataaagtgc acatcacaaa tgccgcggtg cagggcggtt tttacgggcc taacgccgaa 1921 gagttgggcg gttggtttgc ctatccgggc aatggacaaa cgaaaaatgc gcaaacttca 1981 tccggcaatg gaaattcagc aggcagcgcg accgtggtat tcggtgcgaa acgccaacag 2041 cttgtgaaat aa [SEQ ID NO:86] Neisseria gonorrhoeae pilus biogenesis gene cluster, pilO, pilP and pilQ genes, complete cds. ACCESSION   U40596 VERSION     U40596.1  GI:1173872 source : Neisseria gonorrhoeae/strain=“MS11” gene=“pilO” coding sequence 22..669 /protein_id=“AAC43601.1” /db_xref=“GI:1173873”                      /translation= “MASKSSKTNLDLNNLHLLNLPARLFIALLVVAAVLGLGYAGLFK SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKKLPYSISITGNYEQISQFTRDVGSLS RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSIEELAAEAAQNAEQK” [SEQ ID NO:87] gene=“pilP” coding sequence 687..1229 /protein_id=“AAC43602.1” /db_xref=“GI:1173874” /translation= “MKHYALLISFLALSACSQSSEDLNEWMAQTRREAKAEIIPFQAP TLPVAPVYSPPQLTGPNAFDFRRMETAKKGENAPDTKRIKETLEKFSLENMRYVGILK SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIILNELIEDSTGNWVSRKAE                      LLLNSSDKNTEQAAQPEEQN” [SEQ ID NO:88] gene=“pilQ” coding sequence 1248..3410 /protein_id=“AAC43603.1” /db_xref=“GI:1173875” /translation= “MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPFTESVV SVSAPFSPAKQQAAASAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQ HDHIIVTLKNHTLPTALQRSLDVADFKTPVQKVTLKRLNNDTQLIITTTGNWELVNKS AAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNI VASDSVSGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNMAPRRAACQRQSLLTS GKRHCRSGRAVFPKLPIEIQKCGRIPQHPALDNADTTGNRNTLVSGRGSVLIDPATNT LIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGRKKLKN ETSAFGWGVNSGFGGGDKWEAKPKSTCRLPCRKQHFAGARDFSGALNLELSASESLSK TKTLANPRVLTQNRKEAKIESGYEIPFTVTTRSGGGNSTNTELKKAVLGLTVTANITP DGQIIMTVKINKDSPRQCASGNNTILCISTKSLNTQAMVENGGTLIVGGIYEENNGNT                      LTKVPLLATSPLSATSLKHSGKNRPPRTADFQLPPREL” [SEQ ID NO:89]    1 aaacgcacag gaggaaactg aatggcttct aaatcatcta aaaccaactt ggatctcaac   61 aaccttcacc tgctcaacct tcctgccagg ctttttatcg ccctgctggt cgttgccgcc  121 gtgctggggc tcggttatgc cggattgttc aaaagccaga tggaatccct tgaggaatat  181 gaagcaaaag aaaccgaact gaaaaacacc tacaaacaga aaagtatcga cgcggccagc  241 ctgaacaacc ttagggacga acttgcctca atccgctctg ccttcgatat catgttgaaa  301 cagctgccga cagatgcaga aattcccaat ttggttcaag agcttcatca ggcgggttcg  361 agcaacggtc tgcgcttgga cagcgttatg ccccaacctc ccgtagatga cggtcccatc  421 aaaaaattac cctattccat ttccattacc ggaaattacg aacagatcag ccaatttacc  481 cgcgatgtcg gcagtctctc ccgaatcatt acccttgagt cgctgaaaat cgcccaatct  541 ccggaaaacg gcggcaatcc tgacggcaag agcagtatcc tgaacctcag cgccattgcc  601 accacctacc aagcaaaatc catagaagag cttgccgcag aagcggcaca aaatgccgag  661 caaaaataac ttacgttagg gaaaccatga aacactatgc cttactcatc agctttctgg  721 ctctctccgc gtgttcccaa agttctgaag acctaaacga atggatggca caaacgcgac  781 gcgaagccaa agcagaaatc atacctttcc aagcacctac cctgccggtt gcgccggtat  841 acagcccgcc gcagcttaca gggccgaacg cattcgactt ccgccgcatg gaaaccgcca  901 aaaaagggga aaatgccccc gacaccaagc gtattaaaga aacgctggaa aaattcagtt  961 tggaaaatat gcgttatgtc ggcattttga agtccggaca gaaagtctcc ggcttcatcg 1021 aggctgaagg ttatgtctac actgtcggtg tcggcaacta tttgggacaa aactacggta 1081 gaatcgaaag cattaccgac gacagcatca tcctgaacga gctgatagaa gacagcacgg 1141 gcaactgggt ttcccgtaaa gcagaactgc tgttgaattc ttccgacaaa aacaccgaac 1201 aagcggcaca gcctgaggaa caaaattaag aagaggatta ctccattatg aataccaaac 1261 tgacaaaaat catttccggt ctctttgtcg caaccgccgc ctttcagacg gcatcggcag 1321 gaaacattac agacatcaaa gtttcctccc tgcccaacaa acagaaaatc gtcaaagtca 1381 gctttgacaa agagattgtc aacccgaccg gcttcgtaac ctcctcaccg gcccgcatcg 1441 ccttggactt tgaacaaacc ggcatttcca tggatcaaca ggtactcgaa tatgccgatc 1501 ctctgttgag caaaatcagt gccgcacaaa acagcagccg tgcgcgtctg gttctgaatt 1561 tgaacaaacc gggccaatac aataccgaag tacgcgggaa caaagtttgg atattcatta 1621 acgaatcgga cgataccgtg tccgcccccg cccgcccagc cgtaaaagcc gcgcctgccg 1681 caccggcaaa acaacaggct gccgcaccgt ttaccgagtc cgtagtatcc gtatccgcac 1741 cgttcagccc ggcaaaacaa caggcagcgg catcggcaaa acaacaggcg gcgacaccgg 1801 caaaacaaac caatatcgat ttccgcaaag acggcaaaaa tgccggcatt atcgaattgg 1861 cggcattggg ctttgccggg cagcccgaca tcagccaaca gcacgaccac atcatcgtta 1921 cgctgaaaaa ccataccctg ccgaccgcgc tccaacgcag tttggatgtg gcagacttca 1981 aaacaccggt tcaaaaggtt acgctgaaac gcctcaataa cgacacccag ctgattatca 2041 caacaaccgg caactgggaa ctcgtcaaca aatccgccgc gcccggatac tttaccttcc 2101 aagtcctgcc gaaaaaacaa aacctcgagt caggcggcgt gaacaatgcg cccaaaacct 2161 tcacaggccg gaaaatctcc cttgacttcc aagatgtcga aatccgcacc atcctgcaga 2221 ttttggcaaa agaatccggg atgaacattg ttgccagcga ctccgtcagc ggcaaaatga 2281 ccctctccct caaagacgta ccttgggatc aggctttgga tttggttatg caggcgcgca 2341 acctcgatat gcgccagcaa gggaacatcg tcaacatggc cccgcgacga gctgcttgcc 2401 aaagacaaag ccttcttaca agcggaaaaa gacattgccg atctgggcgc gctgtattcc 2461 caaaacttcc aattgaaata caaaaatgtg gaagaattcc gcagcatcct gctttggaca 2521 atgccgacac gaccggaaac cgcaacacgc ttgtcagcgg caggggcagc gtgctgatcg 2581 atcccgccac caacaccctg attgttaccg atacccgcag cgtcatcgaa aaattccgca 2641 aactgattga cgaattggac gtacccgcgc aacaagtgat gattgaggcg cgtatcgtcg 2701 aagcggcaga cggcttctcg cgcgatttgg gcgttaagtt cggcgcgaca ggcaggaaaa 2761 aactgaaaaa tgagacgagc gcattcggct ggggcgtgaa ctccggcttc gggggcggcg 2821 ataaatggga ggccaaacca aaatcaacct gccggttgcc gtgccgcaaa cagcatttcg 2881 ctggtgcgcg cgatttctcc ggcgcgttga atttggaatt gtccgcatcc gagtcgcttt 2941 caaaaaccaa aacgcttgcc aatccgcgcg tgctgaccca aaaccgcaaa gaggccaaaa 3001 tcgaatccgg ttacgaaatt ccttttaccg taactacacg ctcgggcggc ggcaactcta 3061 ccaacacgga actcaaaaaa gccgtcttgg ggctgaccgt tacggcgaac atcacgcccg 3121 acggacaaat catcatgacc gtcaaaatca acaaagactc gcctcgacaa tgtgcttcag 3181 gcaacaacac aatcctatgt atttcgacca aaagcctgaa tacgcaggct atggttgaaa 3241 acggcggcac tttgattgtc ggcggtattt atgaagaaaa caacggcaat acgctgacca 3301 aagtccccct gttggctaca tccccgttat cggcaacctc tttaaaacac tcgggaaaaa 3361 accgaccgcc gcgaactgct gattttcaat tacccccgag ggaattatag atacggcgca 3421 acagcctgcg ctattgatgc gtcaaaataa gggcatatgt tttacagcat atgccctttc 3481 tttatgcttt ttgccgcgac cgaaatgccg tcattcccgc gagcgaatcc aacttgtccg 3541 gtttcggttg tttttcgtct cgtaactttt gagccgtcat tcccgcgaaa tcggaaatcc 3601 agtccgttca gtttcggtca tttccgataa attcctgttg cttttcattt ctagattccc 3661 actttcgtgg aataacggcg gaagggataa atcctcgcaa tccaaagcct gctcatttcc 3721 acaaaaaaca gcaacccgaa acaccccgtc attcccgagc aggcggaatc tagaaccgca 3781 acgccaggaa tctgtcggat acggctgaaa ccgaacgact ggattcccg [SEQ ID NO:90] NspA Neisseria gonorrhoeae outer membrane protein gene, complete cds ACCESSION   U52069 VERSION     U52069.1  GI:1808968 source Neisseria gonorrhoeae/strain=“B2” Gene “NspA” coding sequence : 141..665 /protein_id=“AAB41581.1” /db_xref=“GI:1808969”                                       /translation “MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASVIYDFDTQSPVKPYFG ARLSLNRASAHLGGSDSFSKTSAGLGVLAGVSYAVTPNVDLDAGYRYNYVGKVNTVKN                      VRSGELSAGVRVKF” [SEQ ID NO:91]    1 cggcaaagca gccggatgcc gccgcgtatc ttgaggcatt gaaaatatta cgatgcaaaa   61 agaaaatttc agtataatac ggcaggattc tttaacggat tattaacaat ttttctccct  121 gaccataaag gaaccaaaat atgaaaaaag cacttgccgc actgattgcc ctcgcactcc  181 cggccgccgc actggcggaa ggcgcatccg gcttttacgt ccaagccgat gccgcacacg  241 ccaaagcctc aagctcttta ggttctgcca aaggcttcag cccgcgcatc tccgcaggct  301 accgcatcaa cgacctccgc ttcgccgtcg attacacgcg ctacaaaaac tataaagccc  361 catccaccga tttcaaactt tacagcatcg gcgcgtccgt catttacgac ttcgacaccc  421 aatcgcccgt caaaccgtat ttcggcgcgc gcttgagcct caaccgcgct tccgcccact  481 tgggcggcag cgacagcttc agcaaaacct ccgccggcct cggcgtattg gcgggcgtaa  541 gctatgccgt taccccgaat gtcgatttgg atgccggcta ccgctacaac tacgtcggca  601 aagtcaacac tgtcaaaaac gtccgttccg gcgaactgtc cgccggcgtg cgcgtcaaat  661 tctgatatac gcgttattcc gcaaaccgcc gagccttcgg cggttttttg [SEQ ID NO:92] Neisseria gonorrhoeae outer membrane protein (omp85) gene, complete cds. ACCESSION   U81959 VERSION     U81959.1  GI:1766041 Source: Neisseria gonorrhoeae/strain=“FA19” gene=“omp85” coding sequence 1..2379 /protein_id=“AAC17600.1” /db_xref=“GI:1766042” /translation “MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGLLLLTVIVCPTIGSLNITGAK MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSDRFDRQKF AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTRQTIKITVHEGGRFRWGKVSIEGDT NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAGTKT VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDIYGKAFDPRKASTSVKQYKT TTAGGGVRMGIPVTEYDRVNFGLAAEHLTVNTYNKAPKRYADFIKQYGKTDGADGSFK GLLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGRTYTAAENGNNKSVYSENAH KSTFTNELRYSAGGAVTWLSPLGPMKFIYAYPLKKKPEDEIQRFQFQLGTTF” [SEQ ID NO:93]    1 atgaaactga aacagattgc ctccgcactg atgatgttgg gcatatcgcc tttggcattt   61 gccgacttca ccatccaaga catccgtgtc gaaggcttgc agcgtaccga gccgagcacc  121 gtattcaact acctgcccgt caaagtcggc gacacctaca acgacacaca cggcagtgcc  181 atcatcaaaa gcctgtacgc caccggtttc tttgacgacg tacgagtcga aactgcggac  241 gggctgcttc tgctgaccgt tatcgtatgc cctaccatcg gctcgctcaa catcaccggc  301 gccaaaatgc tgcagaacga cgccatcaag aaaaacctcg aatcgttcgg gctggcgcag  361 tcgcaatact ttaatcaggc gacactcaac caggcagtcg ccggcctgaa agaagaatat  421 ctcgggcgcg gcaaactcaa tatccaaatc acgcccaaag taaccaaact cgcccgcaac  481 cgcgtcgaca tcgacatcac gattgacgag ggcaaatccg ccaaaatcac cgacatcgaa  541 tttgaaggca accaagtcta ttccgaccgc aaactgatgc ggcagatgtc gctgaccgaa  601 ggcggcattt ggacatggct gacacgaagc gaccggttcg accgccagaa attcgcccaa  661 gacatggaaa aagtaaccga cttctaccag aacaacggct acttcgattt ccgtatcctc  721 gataccgaca tccaaaccaa cgaagacaaa accaggcaga ccatcaaaat caccgtccac  781 gaaggcggac gtttccgctg gggcaaagtg tcgattgaag gcgacaccaa cgaagtcccc  841 aaggccgaac tggaaaaact gctgaccatg aagcccggca aatggtacga acgccagcag  901 atgaccgccg ttttgggtga gattcagaac cgcatgggct cggcaggcta cgcatacagc  961 gaaatcagcg tacagccgct gccgaacgcc ggaaccaaaa ccgtcgattt cgtcctgcac 1021 atcgaaccgg gcagaaaaat ctacgtcaac gaaatccaca tcaccggcaa caacaaaacc 1081 cgcgacgaag tcgtgcgccg cgaattgcgc caaatggaat ccgcgcctta cgacacctcc 1141 aagctgcaac gctccaaaga gcgcgtcgag cttttgggct acttcgacaa cgtacagttt 1201 gatgccgtcc cgcttgccgg tacgcccgac aaagtcgatt tgaacatgag cctgaccgaa 1261 cgttccaccg gctcgctcga cttgagcgcg ggctgggttc aggataccgg cttggtcatg 1321 tccgccggcg tatcgcagga caacctgttc ggtacgggca agtcggccgc cctgcgcgcc 1381 tcgcgaagca aaaccacgct caacggctcg ctgtcgttta ccgacccgta cttcacggca 1441 gacggggtca gcctgggcta cgatatttac ggaaaagcct tcgacccgcg caaagcatcg 1501 accagcgtca aacaatataa aaccaccacc gccggcggcg gcgtaaggat gggtatcccc 1561 gttaccgaat acgaccgcgt caatttcggg ctggcggcgg aacacctgac cgtcaacacc 1621 tacaacaaag cacccaaacg ctatgccgac tttatcaaac aatacggcaa aaccgacggc 1681 gcagacggca gcttcaaagg cctgctgtac aaaggcactg tcggctgggg gcgcaacaag 1741 accgacagcg ccttatggcc gacgcgcggc tacctgaccg gcgtaaatgc cgaaatcgcc 1801 ctgcccggca gcaaactgca atactactcc gccacccaca accaaacctg gttcttcccc 1861 ttaagcaaaa ccttcacgct gatgctcggc ggcgaagtcg gcattgcggg cggctacggc 1921 agaaccaaag aaatcccctt ctttgaaaac ttctacggcg gcggcctggg ttcggtgcgc 1981 ggctacgaaa gcggcacgct cggcccgaaa gtgtatgacg aatacggcga aaaaatcagc 2041 tacggcggca acaaaaaagc caacgtctcc gccgagctgc tcttcccgat gcccggtgcg 2101 aaagacgcac gcaccgtccg cctgagcctg tttgccgacg caggcagcgt gtgggacggc 2161 agaacctata ccgccgccga aaacggtaac aacaaatcgg tttactcgga aaacgcgcat 2221 aaatccacct ttaccaacga attgcgctat tccgccggcg gcgcggttac ctggctctcg 2281 cctttgggcc cgatgaaatt catctacgcc tacccgctga agaaaaaacc ggaagacgaa 2341 atccaacgct tccaattcca gctcggcacg acgttctaa [SEQ ID NO:94] PldA1 homolog in Neisseria gonorrhoeae Source: U. of Oklahoma sequencing project PldA1-like coding sequence: >GONOCTG01_15 Continuation (15 of 22) of gonoctg01 from base 1400001 ATGAATACACGAAATATGCGCTATATTCTTTTGACAGGACTGTTGCCGACGGCATCCGCT TTTGGAGAGACCGCGCTGCAATGCGCCGCTTTGACGGACAATGTTACGCGTTTGGCGTGT TACGACAGGATTTTTGCGGCACAGCTTCCGTCTTCGGCAGGGCAGGAAGGGCAGGAGTCG AAAGCCGTACTCAATCTGACGGAAACCGTCCGCAGCAGCTTGGATAAGGGCGAGGCGGTC ATTGTTGTTGAAAAAGGCGGGGATGCGCTTCCTGCCGACAGTGCGGGCGAAACCGCCGAT ATCTATACGCCTTTGAGCCTGATGTACGACTTGGACAAAAACGATTTGCGCGGGCTGTTG GGCGTACGCGAACACAATCCGATGTACCTTATGCCGTTTTGGTATAACAATTCGCCCAAC TATGCCCCGAGTTCGCCGACGCGCGGTACGACTGTACAGGAAAAATTCGGACAGCAGAAA CGTGCGGAAACCAAATTGCAGGTTTCGTTCAAAAGCAAAATTGCCGAAAATTTGTTTAAA ACCCGCGCGGATCTGTGGTTCGGCTACACCCAAAGATCCGATTGGCAGATTTACAACCAA GGCAGGAAATCCGCGCCGTTCCGCAATACGGATTACAAACCTGAAATTTTCCTGACCCAG CCTGTGAAGGCGGATTTGCCGTTCGGCGGCAGGCTGCGTATGCTCGGTGCGGGTTTTGTC CACCAGTCCAACGGACAGAGCCGTCCCGAATCGCGTTCGTGGAACAGGATTTATGCCATG GCAGGCATGGAATGGGGCAAATTGACGGTGATTCCGCGCGTGTGGGTGCGTGCGTTCGAT CAGAGCGGCGATAAAAACGACAATCCCGATATTGCCGACTATATGGGGTATGGCGACGTG AAGCTGCAGTACCGCCTGAACGACAGGCAGAATGTGTATTCCGTATTGCGCTACAACCCC AAAACGGGCTACGGCGCGATTGAAGCCGCCTACACGTTTCCGATTAAGGGCAAACTCAAA GGCGTGGTACGCGGATTCCACGGTTACGGCGAGAGCCTGATCGACTACAACCACAAGCAG AACGGTATCGGTATCGGGTTGATGTTCAACGACTGGGACGGCATCTGA [SEQ ID NO:95] PldA1-like amino acid sequence MNTRNMRYILLTGLLPTASAFGETALQCAALTDNVTRLACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSS LDKGEAVIVVEKGGDALPADSAGETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPFWYNNSPNYAPSSP TRGTTVQEKFGQQKRAETKLQVSFKSKIAENLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTDYKPEIFLT QPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLTVIPRVWVRAFDQSGDKNDNPDIA DYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAIEAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGI GLMFNDWDGI. [SEQ ID NO:96] 1000 base pairs upstream PldA1-like sequence (usuable for replacing the promoter for a stronger sequence) >GONOCTG01_15 Continuation (15 of 22) of gonoctg01 from base 1400001TTTTGGCTTCCAGCGTTTCGTTGTTTTCGTACAAGTCGTAAGTCAGCTTCAGATTGTTGG CTTTTTTAAAGTCTTCGACCGTACTCTCGTCAACATAATTCGACCAGTTGTAGATGTTCA GAGTATCGGTGGCAGCGGCTTCGGCATTGGCAGCAGGTGCGCTGCCTGCTTGAGGCTGCA CGGCGTTTTTTTCGCTGCCGCCGCAGGCTGCCAGAGACAGCGCGGCCAAAACGGCTAATA CGGATTTTTTCATACGGGCAGATTCCTGATGAAAGAGGTTGGAAAAAAAGAAAACCCCGC GCCCCATAAACACCCCGGCGCAAGGTTTGGGTATTGTAAAGTAAATTTGTGCAAACTCAA AGCGATATTGGCCTGATTTTCCTAAAAAATTACCCTGTTTCCAAAAAAGGGGGGGAAACG GCCGCCCGATTTTGCCGTTTTTTTGCGCCGTCAGGGTGTCCGACGGGCGGATAGAGAAAA AAGGCTTGCATATAATGTAAACCCCCTTTAAAATTGCGCGTTTACAGAATTTATTTTTCT TTCAGGAGATTCCAATATGGCAAACAGCGCACAAGCACGCAAACGTGCCCGCCAGTCCGT CAAACAACGCGCCCACAACGCTAGCCTGCGTACCGCATTCCGCACCGCAGTGAAAAAAGT ATTGAAAGCAGTCGAAGCAGGCGATAAAGCTGCCGCACAAGCGGTTTACCAAGAGTCCGT CAAAGTCATCGACCGCATCGCCGACAAAGGCGTGTTTCATAAAAACAAAGCGGCTCGCCA CAAAAGCCGCCTGTCTGCAAAAGTAAAAGCACTGGCTTGATTTTTGCAAAACCGCCAAGG CGGTTGATACGCGATAAGCGGAAAACCCTGAAGCCCGACGGTTTCGGGGTTTTCTGTATT TCGGGGGTAAAGTTCGAAATGGCGGAAAGGGTGCGGTTTTTTATCCGAATCCGCTATAAA ATGCCGTTTGAAAACCAATATGCCGACAATGGGGGCGGAG Preferred gonococcal genes to downregulate via process a) include one or more of the following: [SEQ ID NO:97] Neisseria gonorrhoeae iron-regulated outer membrane protein preFrpB (frpB) gene, complete cds. ACCESSION   U13980 VERSION     U13980.1  GI:833694 Source: Neisseria gonorrhoeae/strain=“FA19” gene=“frpB” coding sequence: 318..2459 /protein_id=“AAC43332.1” /db_xref=“GI:833695”                      /translation= “MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD KNWGVRLNSGFAGNNGASYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNDNGGKT VPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFAVSEKNSRITIKR QAPSYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVKGPNH TRIATRSMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSEFEIKDKEKATNEEKKK NRENEKIAKAYRLTNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTHDGKTVSSS SLNPSFGVIWQPREHWSFSASHNYAGRSPRLYDALQTHGKRGIISIADGTKAERARNT EIGFNYNDGTFAANGSYFRQTIKDALANPQNRHDSVAVREAVNAGYIKNHGYELGASY RTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFKNPNLEIGW RGRYVQKAVGSILAAGQKDRDGKLENVVRQGFGVNDVFANWKPLGKDTLNVNLSVNNV                      FDKFYYPHSQRWTNTLPGVGRDVRLGVNYKF” [SEQ ID NO:98]    1 aaaccggtac ggcgttgccc cgccttagct caaagagaac gattccctaa ggtgctgaag   61 caccgagtga atcggttccg tactatttgt actgtctgcg gcttcgccgc cttgtcctga  121 tttttgttag tccacatata catttccgac aaaacctgtc aacaaaaaac aacgcttcgc  181 aaataaaaac gataatcagc tttacacaac ccccccccgc taatataaac aaaaataatt  241 attattattt tttcttatcc tgccaaacct taacggtttg gcttaacttc ccttcataca  301 ctcaaaagga cgaacaaatg aacgccccgt ttttccgcct cagcctgctc tcgctcacac  361 ttgccgccgg ctttgcccac gcggcagaaa ataatgccaa tgtcgcattg gataccgtta  421 ccgtaaaagg cgaccgccaa ggcagcaaaa tccgtaccaa catcgttacg cttcaacaaa  481 aagacgaaag caccgcaacc gatatgcgcg aactcttaaa agaagagccc tccatcgatt  541 tcggcggcgg caacggcacg tcccaattcc tgacgctgcg cggtatgggt cagaactctg  601 tcgacatcaa ggtggacaac gcctattccg acagccaaat cctttaccac caaggcagat  661 ttattgtcga tcccgctttg gttaaagtcg tttccgtaca gaaaggcgcg ggttccgcct  721 ctgccggtat cggcgcgacc aacggcgcga tcatcgccaa aaccgtcgat gcccaagacc  781 tgctcaaagg cttggataaa aactggggcg tgcgcctcaa cagcggcttt gccggcaaca  841 acggcgcaag ctacggcgca agcgtattcg gaaaagaggg caacttcgac ggtttgttct  901 cttacaaccg caacgatgaa aaagattacg aagccggcaa aggtttccgc aatgacaacg  961 gcggcaaaac cgtaccgtac agcgcgctgg acaaacgcag ctacctcgcc aaaatcggaa 1021 caaccttcgg cgacggcgac caccgcatcg tgttgagcca tatgaaagac caacaccggg 1081 gcatccgcac tgtgcgtgaa gagtttgccg tcagcgaaaa aaattcacgg ataactatta 1141 aacgccaagc cccatcctac cgcgaaacca ctcaatccaa caccaacttg gcgtacaccg 1201 gcaaagattt gggctttgtc gaaaaactgg atgccaacgc ctatgtgttg gaaaagaaac 1261 gctattccgc cgatgacaaa gataacggct acgcaggcaa tgtaaaaggc cccaaccata 1321 cccgaatcgc cactcggagt atgaacttca acttcgacag ccgccttgcc gaacaaaccc 1381 tgttgaaata cggcatcaac taccgccatc aggaaatcaa accgcaagcg tttttgaact 1441 cggaatttga aataaaagat aaagaaaaag caactaatga agagaaaaag aagaaccgtg 1501 aaaatgaaaa aattgccaaa gcctaccgcc tgaccaaccc gaccaaaacc gataccggcg 1561 cgtatatcga agccattcac gagattgacg gctttaccct gaccggcggg ctgcgttacg 1621 accgcttcaa ggtgaaaacc cacgacggca aaaccgtttc aagcagcagc ctcaacccga 1681 gtttcggcgt gatttggcag ccgcgcgaac actggagctt cagcgcgagc cacaactacg 1741 ccggccgcag cccgcgcctg tatgacgctc tgcaaaccca cggcaagcgc ggcatcatct 1801 cgattgccga cggcacgaaa gccgaacgcg cgcgcaatac cgaaatcggc ttcaactaca 1861 acgacggcac gtttgccgca aacggcagct acttccggca gaccatcaaa gacgcgcttg 1921 ccaatccgca aaaccgccac gactccgtcg ccgtccgcga agccgtcaac gccggctaca 1981 tcaaaaacca cggttacgaa ttgggcgcgt cctaccgcac cggcggcctg accgccaaag 2041 tcggcgtaag ccacagcaaa ccgcgctttt acgatacgca caaagacaag ctgttgagcg 2101 cgaaccctga atttggcgca caagtcggcc gcacttggac ggcctccctt gcctaccgct 2161 tcaaaaaccc gaatctggaa atcggctggc gcggtcgtta tgttcaaaaa gccgtgggtt 2221 cgatattggc ggcaggtcaa aaagaccgcg acggcaaatt ggaaaacgtt gtacgccaag 2281 gtttcggtgt gaacgatgtc ttcgccaact ggaaaccgct gggcaaagac acgctcaatg 2341 ttaatctttc ggttaacaac gtgttcgaca agttctacta tccgcacagc caacgctgga 2401 ccaataccct gccgggcgtg ggacgtgatg tacgcctggg cgtgaactac aagttctaaa 2461 acgcacatcc cgaaaaaatg ccgtctgaaa gcctttcaga cggcatctgt cctgataatt 2521 tgatatatag tggattaaca aaaaccggta cggcgttgcc ccgccttagc tcaaagggaa 2581 cgattcccta aggtgctgaa [SEQ ID NO:99] N. gonorrhoeae structural gene for gonococcal protein III (PIII). ACCESSION   X05105 VERSION     X05105.1  GI:44889 source:Neisseria gonorrhoeae/db_xref=“taxon:485” Gene PIII coding sequence: 103..813 /protein_id=“CAA28752.1“ /db_xref=“GI:44890” /db_xref=“SWISS-PROT:P07050” /translation= “MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY GECWKNAYFDKASQGRVECGDAVAVPEPEPAPVAVVEQAPQYVDETISLSAKTLFGFD KDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSEKYNQALSERRAYVVANNLV SNGVPASRISAVGLGESQAQMTQVCQAEVAKLGAKASKAKKREALIACIEPDRRVDVK                      IRSIVTRQVVPARNHHQH” [SEQ ID NO:100]    1 gaattcctat ccgatttgcc gccatgtttc tacagcggcc tgtatgttgg caattcagca   61 gttgcttctg tatctgctgt acaaatctaa tgagggaata aaatgaccaa acagctgaaa  121 ttaagcgcat tattcgttgc attgctcgct tccggcactg ctgttgcggg cgaggcgtcc  181 gttcagggtt acaccgtaag cggccagtcg aacgaaatcg tacgcaacaa ctatggagaa  241 tgctggaaaa acgcctactt tgataaagca agccaaggtc gcgtagaatg cggcgatgcg  301 gttgccgtcc ccgagcccga acccgcgcct gtcgccgttg tggagcaggc tcctcaatat  361 gttgatgaaa ccatttccct gtctgccaaa accctgttcg gtttcgataa ggattcattg  421 cgcgccgaag ctcaagacaa cctgaaagta ttggcgcaac gcctgagtcg aaccaatgtc  481 caatctgtcc gcgtcgaagg ccataccgac tttatgggtt ctgaaaaata caatcaggct  541 ctgtccgaac gccgcgcata cgtagtggca aacaacctgg tcagcaacgg cgtacctgct  601 tctagaattt ctgctgtcgg cttgggcgaa tctcaagcgc aaatgactca agtttgtcaa  661 gccgaagttg ccaaactggg tgcgaaagcc tctaaagcca aaaaacgtga ggctctgatt  721 gcatgtatcg aacctgaccg ccgcgtagat gtgaaaatcc gcagcatcgt aacccgtcag  781 gttgtgccgg cacgcaatca tcaccaacac taaggctagg taatatcttg ccgatgcatg  841 aggttagcgg attttgtacc gggtactgtt gcaatattcg tgaaacgtcg gccggtatcg  901 atgatgtgaa acaaaccccg cttttgcggg gtttgttttt ttgggtggtt ttctgaaacg  961 gctatcgtca gaatcggggt gcaggttcgg attcggattc agattcatgt ttgtgtccca 1021 ttgccgcgct ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt 1081 acaatagtac ggcaaggcga ggcaacgccg taccggttta aatttaatcc actatatcgg 1141 ttgaaactct gattttaagg cggtaggatg tgggtttgcc catagcaagg gaatcctttc 1201 tgtatcaagc cccgaaaggg ataattcata caaattcacg cctttccccc tcattgggaa 1261 atggatggaa tcgtgcccga tgtgtgcggc actgtatgcc ggatatggtt ttatcatcat 1321 cccttttcgg ttgaaacccc gcggaattc

[0116] Preferred Pseudomonas aeruginosa bleb Preparations

[0117] One or more of the following genes (encoding protective antigens) are preferred for upregulation via process b): PcrV, OprF, OprI. They are also preferred as genes which may be heterologously introduced into other Gram-negative bacteria.

[0118] Preferred Moraxella catarrhalis bleb Preparations

[0119] One or more of the following genes (encoding protective antigens) are preferred for upregulation via process b): OMP106 (WO 97/41731 & WO 96/34960), HasR (PCT/EP99/03824), PilQ (PCT/EP99/03823), OMP85 (PCT/EP00/01468), lipo06 (GB 9917977.2), lipo10 (GB 9918208.1), lipo11 (GB 9918302.2), lipo18 (GB 9918038.2), P6 (PCT/EP99/03038), ompCD, CopB (Helminen Me., et al (1993) Infect. Immun. 61:2003-2010), D15 (PCT/EP99/03822), OmplA1 (PCT/EP99/06781), Hly3 (PCT/EP99/03257), LbpA and LbpB (WO 98/55606), ThpA and TbpB (WO 97/13785 & WO 97/32980), OmpE, UspA1 and UspA2 (WO 93/03761), FhaB (WO 99/58685) and Omp21. They are also preferred as genes which may be heterologously introduced into other Gram-negative bacteria.

[0120] One or more of the following genes are preferred for downregulation via process a): CopB, OMP106, OmpB1, ThpA, TbpB, LbpA, and LbpB.

[0121] One or more of the following genes are preferred for downregulation via process c): htrB, msbB and lpxK (most preferably msbB).

[0122] One or more of the following genes are preferred for upregulation via process d): pmrA, pmrB, pmrE, and pmrF.

[0123] Many of the above open reading frames and upstream regions are described in WO 01/09350 (incorporated by reference herein).

[0124] Preferred Haemophilus influenzae bleb Preparations

[0125] One or more of the following genes (encoding protective antigens) are preferred for upregulation via process b): D15 (WO 94/12641), P6 (EP 281673), TbpA, TbpB, P2, P5 (WO 94/26304), OMP26 (WO 97/01638), HMW1, HMW2, HMW3, HMW4, Hia, Hsf, Hap, Hin47, Iomp1457 (GB 0025493.8), YtfN (GB 0025488.8), VirG (GB 0026002.6), Iomp1681 (GB 0025998.6), OstA (GB 0025486.2) and Hif (all genes in this operon should be upregulated in order to upregulate pilin). They are also preferred as genes which may be heterologously introduced into other Gram-negative bacteria.

[0126] One or more of the following genes are preferred for downregulation via process a): P2, P5, Hif, IgA1-protease, HgpA, HgpB, HMW1, HMW2, Hxu, TbpA, and TbpB.

[0127] One or more of the following genes are preferred for downregulation via process c): htrB, msbB and lpxK (most preferably msbB).

[0128] One or more of the following genes are preferred for upregulation via process d): pmrA, pmrB, pmrE, and pmrF.

[0129] Many of the above open reading frames and upstream regions are described in WO 01/09350 (incorporated by reference herein).

[0130] Preparations of Membrane Vesicles (blebs) of the Invention

[0131] The manufacture of bleb preparations from any of the aforementioned modified strains may be achieved by harvesting blebs naturally shed by the bacteria, or by any of the methods well known to a skilled person (e.g. as disclosed in EP 301992, U.S. Pat. No. 5,597,572, EP 11243 or U.S. Pat. No. 4,271,147). For Neisseria, the method described in the Example below is preferably used

[0132] A preparation of membrane vesicles obtained from the bacterium of the invention is a further aspect of this invention. Preferably, the preparation of membrane vesicles is capable of being filtered through a 0.22 μm membrane.

[0133] A sterile (preferably homogeneous) preparation of membrane vesicles obtainable by passing the membrane vesicles from the bacterium of the invention through a 0.22 μm membrane is also envisaged.

[0134] Vaccine Formulations

[0135] A preferred embodiment of the invention is the formulation of the bleb preparations of the invention in a vaccine which may also comprise a pharmaceutically acceptable excipient.

[0136] Vaccine preparation is generally described in Vaccine Design (“The subunit and adjuvant approach” (eds Powell M. F. & Newman M. J.) (1995) Plenum Press New York).

[0137] The bleb preparations of the present invention may be adjuvanted in the vaccine formulation of the invention. Suitable adjuvants include an aluminium salt such as aluminum hydroxide gel (alum) or aluminium phosphate, but may also be a salt of calcium (particularly calcium carbonate), iron or zinc, or may be an insoluble suspension of acylated tyrosine, or acylated sugars, cationically or anionically derivatised polysaccharides, or polyphosphazenes.

[0138] Suitable Th1 adjuvant systems that may be used include, Monophosphoryl lipid A, particularly 3-de-O-acylated monophosphoryl lipid A, and a combination of monophosphoryl lipid A, preferably 3-de-O-acylated monophosphoryl lipid A (3D-MPL) together with an aluminium salt. An enhanced system involves the combination of a monophosphoryl lipid A and a saponin derivative particularly the combination of QS21 and 3D-MPL as disclosed in WO 94/00153, or a less reactogenic composition where the QS21 is quenched with cholesterol as disclosed in WO96/33739. A particularly potent adjuvant formulation involving QS21 3D-MPL and tocopherol in an oil in water emulsion is described in WO95/17210 and is a preferred formulation.

[0139] The vaccine may comprise a saponin, more preferably QS21. It may also comprise an oil in water emulsion and tocopherol. Unmethylated CpG containing oligo nucleotides (WO 96/02555) are also preferential inducers of a TH1 response and are suitable for use in the present invention.

[0140] The vaccine preparation of the present invention may be used to protect or treat a mammal susceptible to infection, by means of administering said vaccine via systemic or mucosal route. These administrations may include injection via the intramuscular, intraperitoneal, intradermal or subcutaneous routes; or via mucosal administration to the oral/alimentary, respiratory, genitourinary tracts. Thus one aspect of the present invention is a method of immunizing a human host against a disease caused by infection of a gram-negative bacteria, which method comprises administering to the host an immunoprotective dose of the bleb preparation of the present invention.

[0141] The amount of antigen in each vaccine dose is selected as an amount which induces an immunoprotective response without significant, adverse side effects in typical vaccinees. Such amount will vary depending upon which specific immunogen is employed and how it is presented. Generally, it is expected that each dose will comprise 1-100 μg of protein antigen, preferably 5-50 μg, and most typically in the range 5-25 μg.

[0142] An optimal amount for a particular vaccine can be ascertained by standard studies involving observation of appropriate immune responses in subjects. Following an initial vaccination, subjects may receive one or several booster immunisations adequately spaced.

[0143] Ghost or Killed Whole Cell Vaccines

[0144] The inventors envisage that the above modified bacterial strains may not only be useful in generating bleb preparations useful in vaccines—they may also be easily used to make ghost or killed whole cell preparations and vaccines (with identical advantages). Methods of making ghost preparations (empty cells with intact envelopes) from Gram-negative strains are well known in the art (see for example WO 92/01791). Methods of killing whole cells to make inactivated cell preparations for use in vaccines are also well known. The terms ‘bleb preparations’ and ‘bleb vaccines’ as well as the processes described throughout this document are therefore applicable to the terms ‘ghost preparation’ and ‘ghost vaccine’, and ‘killed whole cell preparation’ and ‘killed whole cell vaccine’, respectively, for the purposes of this invention.

EXAMPLES

[0145] The examples below are carried out using standard techniques, which are well known and routine to those of skill in the art, except where otherwise described in detail. The examples are illustrative, but do not limit the invention.

Example 1 Previously Reported Examples

[0146] Examples describing: Construction of a Neisseiria meningitidis serogroup B strain lacking capsular polysaccharides; Construction of versatile gene delivery vectors (the pCMK series) targeting integration in the porA locus of Neisseiria meningitidis; Construction of a Neisseiria meningitidis serogroup B strain lacking both capsular polysaccharides and the major immunodominant antigen PorA; Up-regulation of the NspA outer membrane protein production in blebs derived from a recombinant Neisseiria meningitidis serogroup B strain lacking functional porA and cps genes; Up-regulation of the D15/Omp85 outer membrane protein antigen in blebs derived from a recombinant Neisseiria meningitidis serogroup B strain lacking functional cps genes but expressing PorA; Construction of versatile promoter delivery vectors; Fermentation processes for producing recombinant blebs; Identification of bacterial promoters suitable for up-regulation antigens-coding genes; Up-regulation of the N. meningitidis serogroup B Omp85 gene by promoter replacement; Up-regulation of the Hsf protein antigen in a recombinant Neisseiria meningitidis serogroup B strain lacking functional cps genes but expressing PorA; Expression of the Green Fluorescent Protein in a recombinant Neisseria meningitidis serogroup B strain lacking functional cps genes but expressing PorA; Up-regulation of the N. meningitidis serogroup B NspA gene by promoter replacement; Up-regulation of the N. meningitidis serogroup B pldA (omplA) gene by promoter replacement; Up-regulation of the N. meningitidis serogroup B tbpA gene by promoter replacement; Up-regulation of the N. meningitidis serogroup B pilQ gene by promoter replacement; Construction of a kanR/sacB cassette for introducing “clean”, unmarked mutations in the N. meningitidis chromosome; Use of small recombinogenic sequences (43 bp) to allow homologous recombination in the chromosome of Neisseria meningitidis; Active protection of mice immunized with WT and recombinant Neisseria meningitidis blebs; and Immunogenicity of recombinant blebs measured by whole cell & specific ELISA methods have been described in WO 01/09350 (incorporated by reference herein).

Example 2 Gonococcal blebs Expressing Chlamydia trachomatis Proteins on its Surface for use in a Vaccine Composition

[0147] Both Chlamydia trachomatis and N. gonorrhoeae cause sexually transmitted diseases, including urethritis, cervicitis, salpingitis and pelvic inflammatory disease. Mixed infection with both CT and GC does occur. Therefore, in the design of a vaccine targeting one, or more of these diseases, the possibility to afford protection against both organisms with one single formulation creates a technical advantage.

[0148] Protection Against N. gono.

[0149] A N. gonorrhoeae OMV vaccine can be obtained from bleb producing strain(s) in which the expression of one or several genes have been up and/or down regulated. A list of genes encoding N. gonorrhoeae proteins for which it is particularly useful to up/down regulate expression is provided above.

[0150] A successful vaccine for the prevention of infection by N. gono may require more than one of the following elements: generation of serum and/or mucosal antibodies to facilitate complement mediated killing of the gonococcus, and/or to enhance phagocytosis and microbial killing by leukocytes such as polymorphonuclear leukocytes, and/or to prevent attachment of the gonococci to the host tissues; induction of a cell mediated immune response may also participate to protection.

[0151] The potential of a bleb gono vaccine preparation can be evaluated by analyzing the induced immune response for serum and/or mucosal antibodies that have antiadherence, and/or opsonizing properties, and/or bactericidal activity, as described by others (McChesney D et al, Infect. Immun. 36: 1006, 1982; Boslego J et al: Efficacy trialof a purified gonococcl pilus vaccine, in Program and Abstracts of the 24^(th) Interscience Conference on Antimicrobial Agents and Chemotherapy, Whashington, American Society for Microbiology, 1984; Siegel M et al, J. Infect. Dis 145: 300, 1982; de la Pas, Microbiology, 141 (Pt4): 913-20, 1995).

[0152] A mouse model of genital infection by N. gono has recently been described (Plante M, J. Infect. Dis., 182: 848-55, 2000). The efficiency of a bleb gono vaccine could also be evaluated by its ability to prevent or to reduce colonization by N. gono in this mouse model of infection.

[0153] Protection Against CT

[0154] A GC/CT bleb vaccine can be obtained from a strain expressing one or several Chlamydia genes, preferably selected from the above list of genes encoding predicted outer membrane proteins.

[0155] Other genes of interest for overexpression in Neisseria are C. trachomatis genes for which no homolog has been found in C. pneumoniae. Such a set of genes has been described in Richard S.; p:9-27, Stephens Stephens Ed. ASM Press, Washington D.C., Chlamydia: Intracellular Biology, Pathogenesis, and Immunity ISBN: 1-55581-155-8 pages: 380.

[0156] Most preferred combinations of Chlamydia trachomatis genes are as follows:

[0157] Major outer membrane protein MOMP (from one or several different serovars) and the Outer membrane Protein Analog (also known as PorB), MOMP (from one or several different serovars) and the Putative Outer Membrane Protein G (pmpG); & PorB and pmpG.

[0158] Although the immunity to CT is not fully understood, there is evidence that Ab play a role in protection. Ab to CT in genital fluids have been associated with immunity to CT (Brunham R C, Infect Immun. March 1983;39(3):1491-4.). A protective role of serum antibody in immunity to chlamydial genital infection has also been shown (Rank R G, Infect Immun. January 1989;57(1):299-301.). Antibodies, e.g. MOMP specific antibodies, have been shown to be capable to neutralize CT infection in vitro and in vivo (Caldwell et al. 1982 Infect. Immun. 38: 745-54, Lucero et al, 1985, Infect. Immun. 50: 595-97, Zhang et al. 1987 J. Immunol. 138: 575-581). The MOMP surface antigen of CT has been shown to bear non linear surface epitopes which are target of neutralizing antibodies (Fan J, J. Infect Dis 1997, 176(3):713-21).

[0159] Thus, an important objective in the design of a protective chlamydia vaccine includes the identification of formulation(s) of the CT antigens able to optimize the induction of a chlamydia specific antibody responses. Optimization of the Ab response includes targeting to the genital mucosa, and/or presentation of properly folded Chlamydia antigens, and/or combination of several antibody targets.

[0160] Mucosal targeting of the immune response to Chlamydia antigen can be achieved by mucosal administration of the vaccine. Intranasal administration of a outer membrane vesicle vaccine can induce persistent local mucosal antibodies and serum antibodies with strong bactericidal activity in humans.

[0161] For certain B cell epitopes, such as non linear epitopes, the presentation of the antigen to the immune system in a properly folded manner is critical. A bleb vaccine prepared from a strain expressing Chlamydia antigen(s) offers to chlamydia OMP an outer membrane environment which can be critical to maintain these antigens in a properly folded structure.

[0162] Combination of several antibody targets can create an increased efficacy by tackling the infection at different steps of the life cycle of the bacteria, such as adhesion to the host cell, internalization by the host cell and/or interference with further steps of the intracellular development.

[0163] The induction and recruitement of Th1 cells into the local genital mucosae are important for immunity against Chlamydia. Thus, an important objective in designing a protective anti-chlamydia vaccine includes the identification of formulation(s) of CT antigen(s) able to optimize the induction of chlamydia speicific Th1 cells, and preferably recruitment of these cells into the genital mucosae. A bleb vaccine prepared from a strain expressing chlamydia antigen(s) can induce a chlamydia specific CMI response. Antigen-specific T-cell responses can be induced in humans after intranasal immunization with an outer membrane vesicle vaccine.

[0164] A particular advantage of a GC/CT bleb vaccine is its capability to induce both Ab and CMI responses.

[0165] The efficacy of the GC/CT bleb vaccine can be evaluated by its ability to elicit Ag or Chlamydia-specific Ab and/or CMI responses. Ab responses can be evaluated by classical techniques such as ELISA or western blot. Preferably, the induced antibodies can neutralize the infectivity of Chlamydia in an in vitro assay (Byrne G. et al. (J Infect Dis. August 1993;168(2):415-20). Preferably, the CMI response is biased toward the Th1 phenotype. A Th1 biased immune response can be assessed by elevated antigen-specific IgG2a/ IgG1 ratios in mice (Snapper et al. 1987, Science 236:944-47). Elevated ratio of Th1/Th2 cytokine, e.g. elevated IFN-gamma/IL-5, ratio upon in vitro restimulation of immune T cells with the antigen(s) can also indicate such a biased Th1 response.

[0166] The ability of the formulation to elicit Ag specific mucosal Ab is of particular interest, and can be demonstrated by detection of antibodies, such as IgG and/or IgA in mucosal fluids, such as genital tract secretions, vaginal lavages. To this end, certain route of administration of the vaccine may be particularly desired such as intranasal, oral, intravaginal, intradermal, deliveries.

[0167] The efficacy of the GC/CT bleb vaccine can be evaluated by its ability to induce protection against a Chlamydia challenge in animal model(s). Examples of such animal models have been described in the literature: genital infection with MoPn in mice (Barron et al. J. Infect. Dis. 1143:63-66), genital infection with human strains in mice (Igietseme et al.2000, Infect. Immun. 68:6798-806, Tuffrey et al. 1992 J. Gen. Microbiol. 138: 1707-1715), Tuffrey), genital infection with GPIC strain in guinea pigs (Rank et al. 1992 Am. J. Pathol. 140:927-936). Protection against infection can be assessed by reduction of shed Chlamydia from the infected site and/or reduction of the histopathological reactions after a challenge infection in immunized animals.

[0168] The advantage of combining two or more Chlamydia antigens (as described above) can be evaluated by one or more of the following techniques:

[0169] Ability to elicit a multi-target Ab and/or T cell protective response

[0170] Ability to elicit Ab titers in an in vitro neutralizing assay, and/or neutralizing Ab against multiple strains (antigenically distinct)

[0171] Ability to elicit a protective immune response against Chlamydia in a mouse model of genital infection as assessed by reduced shedding of bacteria and/or pathology after challenge.

Example 3 Expression of Heterologous Antigens (Clamydia trachomatis MOMP and PorB) in blebs Derived from a Recombinant Neisseiria meningitidis Serogroup B Strain Lacking Functional porA and cps Genes

[0172] Other genes of interest for over-expression in Neisseria are Chlamydia trachomatis genes for which no homologue has been found in Chlamydia pneumoniae. Among those, the major outer membrane protein (MOMP) and the outer membrane protein analog (PorB) have been shown to play a protective role against chlamydial genital infection. Optimization of the Ab response could be achieved by presentation of properly folded proteins.

[0173] MenB bleb vesicles may be used as delivery vectors to express heterologous membrane protein antigens under the control of the engineered porA-lacO promoter described in WO 01/09350. Expressed in the bleb context, recombinant MOMP and PorB from Chlamydia trachomatis serovar D and K can be correctly folded in the membrane and exposed at the surface. Neisseiria meningitidis strains lacking functional cps genes are advantageously used as recipient strains to express the heterologous antigens (WO 01/09350).

[0174] PCR Amplifications of the Genes Coding for MOMP (Chlamydia trachomatis).

[0175] Murine McCoy cells (ATCC) infected either, with Chlamydia trachomatis Serovar K (UW31-CX-serK), or Serovar D (UW31-CX-serD), were lysed in 400 μl of lysis buffer: 50 mM KCl, 10 mM Tris-HCl pH 8.3, 2.5 mM MgCl2, 0.45% Nonidet P40, 0.45% Tween 20 containing 60 μg/ml proteinase K, 3 hours at 56° C. Ten μl of the lysate were used as template to amplify the corresponding genes. The gene coding for MOMP (Serovar K) (SEQID N^(o) 1 below) was PCR amplified using the CYK/OMP/5/NDE and CYKD/OMP/3/BG oligonucleotide primers (see table 1). The gene coding for MOMP (Serovar D) (SEQID N^(o) 2 below) was PCR amplified using the CYD/OMP/5/NRU and CYKD/OMP/3/BG oligonucleotide primers (see table 1). The conditions used for PCR amplification were those described by the supplier (HiFi DNA polymerase, Boehringer Mannheim, GmbH). Thermal cycling was the following: 25 times (94° C. 1 min., 52° C. 1 min., 72° C. 3 min.) and 1 time (72° C. 10 min., 4° C. up to recovery). The corresponding amplicons (1194 bp) were digested with either NdeI/BglII or NruI/BglII restriction enzymes and can be cloned in the corresponding restriction sites of pCMK (+) delivery vector (as described in WO 01/09350).

[0176] PCR Amplifications of the Genes Coding for PorB (Chlamydia trachomatis).

[0177] Murine McCoy cells (ATCC) infected either, with Chlamydia trachomatis Serovar K (UW31-CX-serK), or Serovar D (UW31-CX-serD), were lysed in 400 μl of lysis buffer: 50 mM KCl, 10 mM Tris-HCl pH 8.3, 2.5 mM MgCl2, 0.45% Nonidet P40, 0.45% Tween 20 containing 60 μg/ml proteinase K, 3 hours at 56° C. Ten μl of the lysate were used as template to amplified the corresponding genes.

[0178] PorB sequences are highly conserved amongst serovar D and K (SEQID N^(o) 3 below). The same primers were used to amplify the corresponding genes in both serovars: CYD/PORB/5/NRU and CYD/PORB/3/BG (see table 1). The conditions used for PCR amplification were those described by the supplier (HiFi DNA polymerase, Boehringer Mannheim, GmbH). Thermal cycling was the following: 25 times (94° C. 1 min., 52° C. 1 min., 72° C. 3 min.) and 1 time (72° C. 10 min., 4° C. up to recover corresponding amplicons (1035 bp) were digested with NruI/BglII restriction enzymes and can be cloned in the corresponding restriction sites of pCMK (+) delivery vector (as described in WO 01/09350).

[0179] Transformation

[0180] Linearized recombinant pCMK plasmids can be transformed within a Neisseria meningitidis serogroup B strain lacking functional cps genes (described in WO 01/09350). Integration resulting from a double crossing-over between the pCMK vectors and the chromosomal porA locus can be selected by a combination of PCR and Western Blot screening as described in WO 01/09350. TABLE 1 Oligonucleotides used in this work Oligonucleotides Sequence Remark(s) CYK/OMP/5/NDE 5′-GGG AAT CCA TAT GAA AAA ACT CTT GAA NdeI cloning site ATC GG-3′[SEQ ID NO:101] CYKD/OMP/3/BG 5′-GGA AGA TCT TTA GAA GCG GAA TTG TGC Bgl II cloning site AT-3′[SEQ ID NO:102] CYD/OMP/5/NRU 5′-CTG CAG AAT CGC GAA TGA AAA AAC TCT NruI cloning site TGA AAT CGG-3′[SEQ ID NO:103] CYD/POR/5/NRU 5′-CTG CAG AAT CGC GAA TGA GTA GCA AGC NruI cloning site TAG TGA AC-3′[SEQ ID NO:104] CYD/POR/3/BG 5′-AGG AGA TCT TTA GAA TTG GAA TCC TCC Bgl II cloning site GG-3′[SEQ ID NO:105] [SEQ ID NO:106] Nucleotide sequence of DNA coding for Chlamydia trachomatis MOMP serovar K protein. atgaaaaaactcttgaaatcggtattagtatttgccgctttgagttctgcttcctccttgcaagctct gcctgtggggaatcctgctgaaccaagccttatgatcgacggaattctgtgggaaggtttcggcggag atccttgcgatccttgcaccacttggtgtgacgctatcagcatgcgcgttggttactacggagacttt gttttcgaccgtgttttgaaaactgatgtgaataaagaatttcagatgggagcggcgcctactaccag cgatgtagaaggcttacaaaacgatccaacaacaaatgttgctcgtccaaatcccgcttatggcaaac acatgcaagatgctgaaatgtttacgaacgctgcttacatggcattaaatatctgggatcgttttgat gtattttgtacattgggagcaactaccggttatttaagaggaaactccgcttccttcaacttagttgg attattcggaacaaaaacacaatattctaagtttaatacagcgaatcttgttcctaacactgctttgg atcgagctgtggttgagctttatacagacaccacctttgcttggagcgtaggtgctcgtgcagctctc tgggaatgtgggtgtgcaacgttaggagcttctttccaatatgctcaatctaaacctaaagtagaaga gttaaatgttctttgtaatgcatccgaatttactattaataagccgaaaggatatgttggggtggaat ttccacttgatattaccgcaggaacagaagctgcgacagggactaaggatgcctctattgactaccat gagtggcaagcaagtttagccctttcttacagattaaatatgttcactccttacattggagttaaatg gtctagagtaagttttgatgccgacacgatccgtatcgctcagcctaaattggctgaagcaatcttgg atgtcactactctaaacccgaccatcgctggtaaaggagctgtggtctcttccggaagcgataacgaa ctggctgatacaatgcaaatcgtttccttgcagttgaacaagctgaaatctagaaaatcttgcggtat tgcagtaggaacgactattgtagatgcagataaatacgcagttacagttgagactcgcttgatcgatg agagagcagctcacgtaaatgcacaattccgcttctaa [SEQ ID NO:107] Nucleotide sequence of DNA coding for Chlamydia trachomatis MOMP serovar D protein. atgaaaaaactcttgaaatcggtattagtatttgccgctttgagttctgcttcctccttgcaagctct gcctgtggggaatcctgctgaaccaagccttatgatcgacggaattctgtgggaaggtttcggcggag atccttgcgatccttgcgccacttggtgtgacgctatcagcatgcgtgttggttactacggagacttt gttttcgaccgtgttttgaaaactgatgtgaataaagaatttcagatgggtgccaagcctacaactga tacaggcaatagtgcagctccatccactcttacagcaagagagaatcctgcttacggccgacatatgc aggatgctgagatgtttacaaatgccgcttgcatggcattgaatatttgggatcgttttgatgtattc tgtacattaggagccaccagtggatatcttaaaggaaactctgcttctttcaatttagttggattgtt tggagataatgaaaatcaaaaaacggtcaaagcggagtctgtaccaaatatgagctttgatcaatctg ttgttgagttgtatacagatactacttttgcgtggagcgtcggcgctcgcgcagctttgtgggaatgt ggatgtgcaactttaggagcttcattccaatatgctcaatctaaacctaaagtagaagaattaaacgt tctctgcaatgcagcagagtttactattaataaacctaaagggtatgtaggtaaggagtttcctcttg atcttacagcaggaacagatgctgcgacaggaactaaggatgcctctattgattaccatgaatggcaa gcaagtttagctctctcttacagactgaatatgttcactccctacattggagttaaatggtctcgagc aagctttgatgccgatacgattcgtatagcccagccaaaatcagctacagctatttttgatactacca cgcttaacccaactattgctggagctggcgatgtgaaaactggcgcagagggtcagctcggagacaca atgcaaatcgtttccttgcaattgaacaagatgaaatctagaaaatcttgcggtattgcagtaggaac aactattgtggatgcagacaaatacgcagttacagttgagactcgcttgatcgatgagagagcagctc acgtaaatgcacaattccgcttctaa [SEQ ID NO:108] Nucleotide sequence of DNA coding for Chlamydia trachomatis PorB serovar D protein. atgagtagcaagctagtgaactatctccgtttgactttcctatcttttttagggatcgcatctacttc attagacgctatgcctgcggggaatccggcgtttccagtcatcccggggattaatattgaacagaaaa atgcctgttctttcgatttatgtaattcttatgatgtactatccgcactgtccggtaacctgaagctc tgcttctgcggagattatatcttttcagaagaagctcaggtaaaagatgtccctgtcgttacctctgt gacaacagctggggttggtccttctcctgatattacttcgacaaccaaaacgcgaaatttcgatctcg tgaactgtaatctcaatacaaactgtgtagctgtagctttttcccttcctgatcgttcgctgagcgcg attcctctgtttgatgtgagtttcgaagtgaaagtaggaggactgaaacaatactaccgccttcccat gaatgcctatcgagacttcacctcggaacctctcaattctgaatcagaagttacggacgggatgattg aagtacagtccaattacggatttgtttgggatgttagcttgaaaaaagtcatatggaaagatggcgtt tcctttgtaggcgtcggtgcagactatcgccatgcttcttgccctattgactacatcattgcaaacag tcaagctaatccagaagtattcatcgctgactcggatgggaaactgaacttcaaggagtggagtgtct gcgtaggtcttactacctatgtgaatgactacgttcttccttacttagcgttttctatagggagtgtt tctcgccaagctccggacgacagcttcaaaaaattagaagatcgcttcactaacctcaaatttaaagt tcgtaaaattaccagctctcatcgtggaaacatctgcatcggagcgacaaactatgtcgccgataact tcttctacaacgtagaaggaagatggggaagccagcgcgctgtgaacgtctccggaggattccaattc taa

Example 4 Isolation and Purification of blebs from meningococci Devoid of Capsular polysaccharide

[0181] Recombinant blebs can be purified as described below. The cell paste (42 gr) is suspended in 211 ml of 0.1M Tris-Cl buffer pH 8.6 containing 10 mM EDTA and 0.5% Sodium Deoxycholate (DOC). The ratio of buffer to biomass should be 5/1 (V/W). The biomass is extracted by magnetic stirring for 30 minutes at room temperature. Total extract is then centrifuged at 20,000 g for 30 minutes at 4° C. (13,000 rpm in a JA-20 rotor, Beckman J2-HS centrifuge). The pellet should be discarded. The supernatant is ultracentrifuged at 125,000 g for 2 hours at 4° C. (40,000 rpm in a 50.2 Ti rotor, Beckman L8-70M ultracentrifuge) in order to concentrate vesicles. The supernatant should be discarded. The pellet is gently suspended in 25 ml of 50 mM Tris-Cl buffer pH 8.6 containing 2 mM EDTA, 1.2% DOC and 20% sucrose. After a second ultracentrifugation step at 125,000 g for 2 hours at 4° C., vesicles are gently suspended in 44 ml of 3% sucrose and stored at 4° C. All solutions used for bleb extraction and purification contained 0.01% thiomersalate. As illustrated in WO 01/019350, this procedure yields protein preparations highly enriched in outer-membrane proteins.

Example 5 Models for Testing Protection Against gonococcal and C. trachomatis Infection

[0182] This can be done as described above in Example 2. In addition Whittum-Hudson et al. (Vaccine Jul. 16, 2001;19(28-29):4061-71) “The anti-idiotypic antibody to chlamydial glycolipid exoantigen (GLXA) protects mice against genital infection with a human biovar of Chlamydia trachomatis” is a vaginal inoculation model for C. trachomatis (incorporated by reference herein) which can also be used to test vaccine efficacy.

1 108 1 1023 DNA Chlamydia trachomatis 1 atgagtagca agctagtgaa ctatctccgt ttgactttcc tatctttttt agggatcgca 60 tctacttcat tagacgctat gcctgcgggg aatccggcgt ttccagtcat cccggggatt 120 aatattgaac agaaaaatgc ctgttctttc gatttatgta attcttatga tgtactatcc 180 gcactgtccg gtaacctgaa gctctgcttc tgcggagatt atatcttttc agaagaagct 240 caggtaaaag atgtccctgt cgttacctct gtgacaacag ctggggttgg tccttctcct 300 gatattactt cgacaaccaa aacgcgaaat ttcgatctcg tgaactgtaa tctcaataca 360 aactgtgtag ctgtagcttt ttcccttcct gatcgttcgc tgagcgcgat tcctctgttt 420 gatgtgagtt tcgaagtgaa agtaggagga ctgaaacaat actaccgcct tcccatgaat 480 gcctatcgag acttcacctc ggaacctctc aattctgaat cagaagttac ggacgggatg 540 attgaagtac agtccaatta cggatttgtt tgggatgtta gcttgaaaaa agtcatatgg 600 aaagatggcg tttcctttgt aggcgtcggt gcagactatc gccatgcttc ttgccctatt 660 gactacatca ttgcaaacag tcaagctaat ccagaagtat tcatcgctga ctcggatggg 720 aaactgaact tcaaggagtg gagtgtctgc gtaggtctta ctacctatgt gaatgactac 780 gttcttcctt acttagcgtt ttctataggg agtgtttctc gccaagctcc ggacgacagc 840 ttcaaaaaat tagaagatcg cttcactaac ctcaaattta aagttcgtaa aattaccagc 900 tctcatcgtg gaaacatctg catcggagcg acaaactatg tcgccgataa cttcttctac 960 aacgtagaag gaagatgggg aagccagcgc gctgtgaacg tctccggagg attccaattc 1020 taa 1023 2 340 PRT Chlamydia trachomatis 2 Met Ser Ser Lys Leu Val Asn Tyr Leu Arg Leu Thr Phe Leu Ser Phe 1 5 10 15 Leu Gly Ile Ala Ser Thr Ser Leu Asp Ala Met Pro Ala Gly Asn Pro 20 25 30 Ala Phe Pro Val Ile Pro Gly Ile Asn Ile Glu Gln Lys Asn Ala Cys 35 40 45 Ser Phe Asp Leu Cys Asn Ser Tyr Asp Val Leu Ser Ala Leu Ser Gly 50 55 60 Asn Leu Lys Leu Cys Phe Cys Gly Asp Tyr Ile Phe Ser Glu Glu Ala 65 70 75 80 Gln Val Lys Asp Val Pro Val Val Thr Ser Val Thr Thr Ala Gly Val 85 90 95 Gly Pro Ser Pro Asp Ile Thr Ser Thr Thr Lys Thr Arg Asn Phe Asp 100 105 110 Leu Val Asn Cys Asn Leu Asn Thr Asn Cys Val Ala Val Ala Phe Ser 115 120 125 Leu Pro Asp Arg Ser Leu Ser Ala Ile Pro Leu Phe Asp Val Ser Phe 130 135 140 Glu Val Lys Val Gly Gly Leu Lys Gln Tyr Tyr Arg Leu Pro Met Asn 145 150 155 160 Ala Tyr Arg Asp Phe Thr Ser Glu Pro Leu Asn Ser Glu Ser Glu Val 165 170 175 Thr Asp Gly Met Ile Glu Val Gln Ser Asn Tyr Gly Phe Val Trp Asp 180 185 190 Val Ser Leu Lys Lys Val Ile Trp Lys Asp Gly Val Ser Phe Val Gly 195 200 205 Val Gly Ala Asp Tyr Arg His Ala Ser Cys Pro Ile Asp Tyr Ile Ile 210 215 220 Ala Asn Ser Gln Ala Asn Pro Glu Val Phe Ile Ala Asp Ser Asp Gly 225 230 235 240 Lys Leu Asn Phe Lys Glu Trp Ser Val Cys Val Gly Leu Thr Thr Tyr 245 250 255 Val Asn Asp Tyr Val Leu Pro Tyr Leu Ala Phe Ser Ile Gly Ser Val 260 265 270 Ser Arg Gln Ala Pro Asp Asp Ser Phe Lys Lys Leu Glu Asp Arg Phe 275 280 285 Thr Asn Leu Lys Phe Lys Val Arg Lys Ile Thr Ser Ser His Arg Gly 290 295 300 Asn Ile Cys Ile Gly Ala Thr Asn Tyr Val Ala Asp Asn Phe Phe Tyr 305 310 315 320 Asn Val Glu Gly Arg Trp Gly Ser Gln Arg Ala Val Asn Val Ser Gly 325 330 335 Gly Phe Gln Phe 340 3 601 PRT Chlamydia trachomatis 3 Met Lys Met Asn Arg Ile Trp Leu Leu Leu Leu Thr Phe Ser Ser Ala 1 5 10 15 Ile His Ser Pro Val Gln Gly Glu Ser Leu Val Cys Lys Asn Ala Leu 20 25 30 Gln Asp Leu Ser Phe Leu Glu His Leu Leu Gln Val Lys Tyr Ala Pro 35 40 45 Lys Thr Trp Lys Glu Gln Tyr Leu Gly Trp Asp Leu Val Gln Ser Ser 50 55 60 Val Ser Ala Gln Gln Lys Leu Arg Thr Gln Glu Asn Pro Ser Thr Ser 65 70 75 80 Phe Cys Gln Gln Val Leu Ala Asp Phe Ile Gly Gly Leu Asn Asp Phe 85 90 95 His Ala Gly Val Thr Phe Phe Ala Ile Glu Ser Ala Tyr Leu Pro Tyr 100 105 110 Thr Val Gln Lys Ser Ser Asp Gly Arg Phe Tyr Phe Val Asp Ile Met 115 120 125 Thr Phe Ser Ser Glu Ile Arg Val Gly Asp Glu Leu Leu Glu Val Asp 130 135 140 Gly Ala Pro Val Gln Asp Val Leu Ala Thr Leu Tyr Gly Ser Asn His 145 150 155 160 Lys Gly Thr Ala Ala Glu Glu Ser Ala Ala Leu Arg Thr Leu Phe Ser 165 170 175 Arg Met Ala Ser Leu Gly His Lys Val Pro Ser Gly Arg Thr Thr Leu 180 185 190 Lys Ile Arg Arg Pro Phe Gly Thr Thr Arg Glu Val Arg Val Lys Trp 195 200 205 Arg Tyr Val Pro Glu Gly Val Gly Asp Leu Ala Thr Ile Ala Pro Ser 210 215 220 Ile Arg Ala Pro Gln Leu Gln Lys Ser Met Arg Ser Phe Phe Pro Lys 225 230 235 240 Lys Asp Asp Ala Phe His Arg Ser Ser Ser Leu Phe Tyr Ser Pro Met 245 250 255 Val Pro His Phe Trp Ala Glu Leu Arg Asn His Tyr Ala Thr Ser Gly 260 265 270 Leu Lys Ser Gly Tyr Asn Ile Gly Ser Thr Asp Gly Phe Leu Pro Val 275 280 285 Ile Gly Pro Val Ile Trp Glu Ser Glu Gly Leu Phe Arg Ala Tyr Ile 290 295 300 Ser Ser Val Thr Asp Gly Asp Gly Lys Ser His Lys Val Gly Phe Leu 305 310 315 320 Arg Ile Pro Thr Tyr Ser Trp Gln Asp Met Glu Asp Phe Asp Pro Ser 325 330 335 Gly Pro Pro Pro Trp Glu Glu Phe Ala Lys Ile Ile Gln Val Phe Ser 340 345 350 Ser Asn Thr Glu Ala Leu Ile Ile Asp Gln Thr Asn Asn Pro Gly Gly 355 360 365 Ser Val Leu Tyr Leu Tyr Ala Leu Leu Ser Met Leu Thr Asp Arg Pro 370 375 380 Leu Glu Leu Pro Lys His Arg Met Ile Leu Thr Gln Asp Glu Val Val 385 390 395 400 Asp Ala Leu Asp Trp Leu Thr Leu Leu Glu Asn Val Asp Thr Asn Val 405 410 415 Glu Ser Arg Leu Ala Leu Gly Asp Asn Met Glu Gly Tyr Thr Val Asp 420 425 430 Leu Gln Val Ala Glu Tyr Leu Lys Ser Phe Gly Arg Gln Val Leu Asn 435 440 445 Cys Trp Ser Lys Gly Asp Ile Glu Leu Ser Thr Pro Ile Pro Leu Phe 450 455 460 Gly Phe Glu Lys Ile His Pro His Pro Arg Val Gln Tyr Ser Lys Pro 465 470 475 480 Ile Cys Val Leu Ile Asn Glu Gln Asp Phe Ser Cys Ala Asp Phe Phe 485 490 495 Pro Val Val Leu Lys Asp Asn Asp Arg Ala Leu Ile Val Gly Thr Arg 500 505 510 Thr Ala Gly Ala Gly Gly Phe Val Phe Asn Val Gln Phe Pro Asn Arg 515 520 525 Thr Gly Ile Lys Thr Cys Ser Leu Thr Gly Ser Leu Ala Val Arg Glu 530 535 540 His Gly Ala Phe Ile Glu Asn Ile Gly Val Glu Pro His Ile Asp Leu 545 550 555 560 Pro Phe Thr Ala Asn Asp Ile Arg Tyr Lys Gly Tyr Ser Glu Tyr Leu 565 570 575 Asp Lys Val Lys Lys Leu Val Cys Gln Leu Ile Asn Asn Asp Gly Thr 580 585 590 Ile Ile Leu Ala Glu Asp Gly Ser Phe 595 600 4 1806 DNA Chlamydia trachomatis 4 atgaaaatga ataggatttg gctattactg cttacctttt cttctgccat acattctcct 60 gtacaaggag aaagcttggt ttgcaagaat gctcttcaag atttgagttt tttagagcat 120 ttattacagg ttaaatatgc tcctaaaaca tggaaagagc aatacttagg atgggatctt 180 gttcaaagct ccgtttctgc acagcagaag cttcgtacac aagaaaatcc atcaacaagt 240 ttttgccagc aggtccttgc tgattttatc ggaggattaa atgactttca cgctggagta 300 actttctttg cgatagaaag tgcttacctt ccttataccg tacaaaaaag tagtgacggc 360 cgtttctact ttgtagatat catgactttt tcttcagaga tccgtgttgg agatgagttg 420 ctagaggtgg atggggcgcc tgtccaagat gtactcgcta ctctatatgg aagcaatcac 480 aaagggactg cagctgaaga gtcggctgct ttaagaacac tattttctcg catggcctct 540 ttagggcaca aagtaccttc tgggcgcact actttaaaga ttcgtcgtcc ttttggtact 600 acgagagaag ttcgtgtgaa atggcgttat gttcctgaag gtgtaggaga tttggctacc 660 atagctcctt ctatcagggc tccacagtta cagaaatcga tgagaagctt tttccctaag 720 aaagatgatg cgtttcatcg gtctagttcg ctattctact ctccaatggt tccgcatttt 780 tgggcagagc ttcgcaatca ttatgcaacg agtggtttga aaagcgggta caatattggg 840 agtaccgatg ggtttctccc tgtcattggg cctgttatat gggagtcgga gggtcttttc 900 cgcgcttata tttcttcggt gactgatggg gatggtaaga gccataaagt aggatttcta 960 agaattccta catatagttg gcaggacatg gaagattttg atccttcagg accgcctcct 1020 tgggaagaat ttgctaagat tattcaagta ttttcttcta atacagaagc tttgattatc 1080 gaccaaacga acaacccagg tggtagtgtc ctttatcttt atgcactgct ttccatgttg 1140 acagaccgtc ctttagaact tcctaaacat agaatgattc tgactcagga tgaagtggtt 1200 gatgctttag attggttaac cctgttggaa aacgtagaca caaacgtgga gtctcgcctt 1260 gctctgggag acaacatgga aggatatact gtggatctac aggttgccga gtatttaaaa 1320 agctttggac gtcaagtatt gaattgttgg agtaaagggg atatcgagtt atcaacgcct 1380 attcctcttt ttggttttga gaagattcat ccacatcctc gagttcaata ctctaaaccg 1440 atttgtgttt tgatcaatga gcaagacttt tcttgtgctg acttcttccc tgtagttttg 1500 aaagacaatg atcgagctct tattgttggt actcgaacag ctggagctgg aggatttgtc 1560 tttaatgtgc agttcccaaa tagaactgga ataaaaactt gttctttaac aggatcatta 1620 gctgttagag agcatggtgc cttcattgag aacatcggag tcgaaccgca tatcgatctg 1680 ccttttacag cgaatgatat tcgctataaa ggctattccg agtatcttga taaggtcaaa 1740 aaattggttt gtcagctgat caataacgac ggtaccatta ttcttgcgga agatggtagt 1800 ttttaa 1806 5 432 PRT Chlamydia trachomatis 5 Met Lys Lys Tyr Phe Tyr Lys Gly Phe Val Gly Ala Leu Leu Leu Ala 1 5 10 15 Cys Gly Ser Thr Asn Leu Ala Phe Ala Gln Ala Ser Ser Met Asp Ser 20 25 30 Gln Leu Trp Ser Val Glu Asp Leu Asp Ser Tyr Leu Ser Ser Lys Gly 35 40 45 Phe Val Glu Thr Arg Lys Arg Asp Gly Val Leu Arg Leu Ala Gly Asp 50 55 60 Val Arg Ala Arg Trp Ile Tyr Ala Lys Glu Asp Leu Glu Thr Thr Gln 65 70 75 80 Thr Pro Ala Lys Pro Met Leu Pro Thr Asn Arg Tyr Arg Ser Glu Phe 85 90 95 Asn Leu Tyr Val Asp Tyr Thr Ala Ala Asn Ser Trp Met Thr Ser Lys 100 105 110 Met Asn Trp Val Thr Ile Ala Gly Gly Glu Ser Ser Ala Ala Gly Leu 115 120 125 Asp Ile Asn Arg Ala Phe Leu Gly Tyr Arg Phe Tyr Lys Asn Pro Glu 130 135 140 Thr Gln Ala Glu Val Phe Ala Glu Ile Gly Arg Ser Gly Leu Gly Asp 145 150 155 160 Ile Phe Asp Ser Asp Val Gln Phe Asn Ser Asn Phe Asp Gly Ile His 165 170 175 Leu Tyr Ala Ala Arg Arg Ile Ser Glu Lys Leu Pro Phe Thr Met Ile 180 185 190 Val His Gly Gly Pro Phe Val Val Asn Met Ala Glu Lys Glu Tyr Ala 195 200 205 Trp Val Val Glu Ala Ile Leu Asn Lys Leu Pro Gly Asn Phe Val Val 210 215 220 Lys Thr Ser Val Val Asp Trp Asn Thr Leu Thr Ala Lys Thr Asn Asp 225 230 235 240 Pro Ala Asp Ala Ser Ala Ala Gln Pro Ala Lys Pro Asn Thr Lys Tyr 245 250 255 Asp Tyr Leu Val Trp Gln Trp Leu Val Gly Lys Ser Thr Ala Met Pro 260 265 270 Trp Phe Asn Gly Gln Thr Lys Asn Leu Tyr Thr Tyr Gly Ala Tyr Leu 275 280 285 Phe Asn Pro Leu Ala Glu Ile Pro Glu Asn Trp Lys Gln Ser Thr Thr 290 295 300 Pro Thr Thr Lys Ile Thr Asn Gly Lys Glu Asn His Ala Trp Phe Ile 305 310 315 320 Gly Cys Ser Leu Gly Gly Val Arg Arg Ala Gly Asp Trp Ser Ala Thr 325 330 335 Val Arg Tyr Glu Tyr Val Glu Ala Leu Ala Ile Pro Glu Ile Asp Val 340 345 350 Ala Gly Ile Gly Arg Gly Asn Gln Met Lys Tyr Trp Phe Ala Gln Ala 355 360 365 Ile Lys Gln Gly Leu Asp Pro Lys Glu Ser Asn Gly Phe Thr Asn Tyr 370 375 380 Lys Gly Val Ser Tyr Gln Phe Val Met Gly Leu Thr Asp Ser Val Ser 385 390 395 400 Phe Arg Ala Tyr Ala Ala Tyr Ser Lys Pro Ala Asn Asp Asn Leu Gly 405 410 415 Ser Asp Phe Thr Tyr Arg Lys Tyr Asp Leu Gly Leu Ile Ser Ser Phe 420 425 430 6 1299 DNA Chlamydia trachomatis 6 atgaaaaaat acttttataa agggtttgta ggcgcgcttt tattagcttg tgggtctaca 60 aacttggctt ttgcgcaggc tagttcgatg gatagccagc tatggtctgt tgaagattta 120 gattcttatt tgagttccaa aggttttgtc gagactcgta agcgcgatgg agttctacgt 180 ttagctggag atgtccgcgc tcgatggatt tatgcaaaag aggatcttga gacaactcag 240 actcctgcta aacctatgtt acctaccaat cggtatcgta gtgaattcaa tttgtatgtg 300 gattacaccg ctgctaatag ttggatgact tcgaaaatga attgggtaac gattgctggc 360 ggagaatctt ctgcagcagg gttagatatt aatcgtgcct tcttaggata ccgattctac 420 aaaaacccag aaacgcaagc agaagtattt gcagagattg gtcgctctgg attgggagat 480 atttttgatt ccgacgttca gtttaatagt aatttcgacg gaattcattt atacgctgcg 540 cgacgtatta gtgagaaact tcctttcacc atgattgttc atggtggtcc ttttgtcgtg 600 aatatggcag agaaagagta tgcttgggtc gtggaagcta ttttgaataa actcccagga 660 aatttcgttg tgaaaacgag tgttgttgac tggaatacgt taacagcaaa aacgaatgat 720 ccagcagacg caagcgctgc acaaccagct aaacctaata ccaagtacga ttatttagta 780 tggcaatggt tggttgggaa gagcacagct atgccatggt ttaatggaca aacaaaaaat 840 ctttacactt acggagccta tctctttaat ccattagcgg aaataccaga gaactggaaa 900 caatcaacaa ctcctacaac caaaattaca aatggtaagg aaaaccatgc ttggttcatc 960 ggctgctctc taggcggtgt tcgacgagct ggagactggt ctgcaacagt tcgttatgag 1020 tatgttgaag ctttagcgat tccagaaatt gatgtcgcgg gtattggtcg cggaaaccaa 1080 atgaaatatt ggtttgctca agctatcaaa caaggattgg atcctaaaga atctaacggc 1140 tttactaact ataaaggagt ttcctatcag tttgttatgg gtctgacaga ttcggtttct 1200 ttccgagctt atgctgctta ttctaagcct gctaacgata accttggtag cgacttcacc 1260 tatcgtaagt atgacctagg tttaatttct tcattctaa 1299 7 878 PRT Chlamydia trachomatis 7 Met Arg Pro Asp His Met Asn Phe Cys Cys Leu Cys Ala Ala Ile Leu 1 5 10 15 Ser Ser Thr Ala Val Leu Phe Gly Gln Asp Pro Leu Gly Glu Thr Ala 20 25 30 Leu Leu Thr Lys Asn Pro Asn His Val Val Cys Thr Phe Phe Glu Asp 35 40 45 Cys Thr Met Glu Ser Leu Phe Pro Ala Leu Cys Ala His Ala Ser Gln 50 55 60 Asp Asp Pro Leu Tyr Val Leu Gly Asn Ser Tyr Cys Trp Phe Val Ser 65 70 75 80 Lys Leu His Ile Thr Asp Pro Lys Glu Ala Leu Phe Lys Glu Lys Gly 85 90 95 Asp Leu Ser Ile Gln Asn Phe Arg Phe Leu Ser Phe Thr Asp Cys Ser 100 105 110 Ser Lys Glu Ser Ser Pro Ser Ile Ile His Gln Lys Asn Gly Gln Leu 115 120 125 Ser Leu Arg Asn Asn Gly Ser Met Ser Phe Cys Arg Asn His Ala Glu 130 135 140 Gly Ser Gly Gly Ala Ile Ser Ala Asp Ala Phe Ser Leu Gln His Asn 145 150 155 160 Tyr Leu Phe Thr Ala Phe Glu Glu Asn Ser Ser Lys Gly Asn Gly Gly 165 170 175 Ala Ile Gln Ala Gln Thr Phe Ser Leu Ser Arg Asn Val Ser Pro Ile 180 185 190 Ser Phe Ala Arg Asn Arg Ala Asp Leu Asn Gly Gly Ala Ile Cys Cys 195 200 205 Ser Asn Leu Ile Cys Ser Gly Asn Val Asn Pro Leu Phe Phe Thr Gly 210 215 220 Asn Ser Ala Thr Asn Gly Gly Ala Ile Cys Cys Ile Ser Asp Leu Asn 225 230 235 240 Thr Ser Glu Lys Gly Ser Leu Ser Leu Ala Cys Asn Gln Glu Thr Leu 245 250 255 Phe Ala Ser Asn Ser Ala Lys Glu Lys Gly Gly Ala Ile Tyr Ala Lys 260 265 270 His Met Val Leu Arg Tyr Asn Gly Pro Val Ser Phe Ile Asn Asn Ser 275 280 285 Ala Lys Ile Gly Gly Ala Ile Ala Ile Gln Ser Gly Gly Ser Leu Ser 290 295 300 Ile Leu Ala Gly Glu Gly Ser Val Leu Phe Gln Asn Asn Ser Gln Arg 305 310 315 320 Thr Ser Asp Gln Gly Leu Val Arg Asn Ala Ile Tyr Leu Glu Lys Asp 325 330 335 Ala Ile Leu Ser Ser Leu Glu Ala Arg Asn Gly Asp Ile Leu Phe Phe 340 345 350 Asp Pro Ile Val Gln Glu Ser Ser Ser Lys Glu Ser Pro Leu Pro Ser 355 360 365 Ser Leu Gln Ala Ser Val Thr Ser Pro Thr Pro Ala Thr Ala Ser Pro 370 375 380 Leu Val Ile Gln Thr Ser Ala Asn Arg Ser Val Ile Phe Ser Ser Glu 385 390 395 400 Arg Leu Ser Glu Glu Glu Lys Thr Pro Asp Asn Leu Thr Ser Gln Leu 405 410 415 Gln Gln Pro Ile Glu Leu Lys Ser Gly Arg Leu Val Leu Lys Asp Arg 420 425 430 Ala Val Leu Ser Ala Pro Ser Leu Ser Gln Asp Pro Gln Ala Leu Leu 435 440 445 Ile Met Glu Ala Gly Thr Ser Leu Lys Thr Ser Ser Asp Leu Lys Leu 450 455 460 Ala Thr Leu Ser Ile Pro Leu His Ser Leu Asp Thr Glu Lys Ser Val 465 470 475 480 Thr Ile His Ala Pro Asn Leu Ser Ile Gln Lys Ile Phe Leu Ser Asn 485 490 495 Ser Gly Asp Glu Asn Phe Tyr Glu Asn Val Glu Leu Leu Ser Lys Glu 500 505 510 Gln Asn Asn Ile Pro Leu Leu Thr Leu Ser Lys Glu Gln Ser His Leu 515 520 525 His Leu Pro Asp Gly Asn Leu Ser Ser His Phe Gly Tyr Gln Gly Asp 530 535 540 Trp Thr Phe Ser Trp Lys Asp Ser Asp Glu Gly His Ser Leu Ile Ala 545 550 555 560 Asn Trp Thr Pro Lys Asn Tyr Val Pro His Pro Glu Arg Gln Ser Thr 565 570 575 Leu Val Ala Asn Thr Leu Trp Asn Thr Tyr Ser Asp Met Gln Ala Val 580 585 590 Gln Ser Met Ile Asn Thr Ile Ala His Gly Gly Ala Tyr Leu Phe Gly 595 600 605 Thr Trp Gly Ser Ala Val Ser Asn Leu Phe Tyr Ala His Asp Ser Ser 610 615 620 Gly Lys Pro Ile Asp Asn Trp His His Arg Ser Leu Gly Tyr Leu Phe 625 630 635 640 Gly Ile Ser Thr His Ser Leu Asp Asp His Ser Phe Cys Leu Ala Ala 645 650 655 Gly Gln Leu Leu Gly Lys Ser Ser Asp Ser Phe Ile Thr Ser Thr Glu 660 665 670 Thr Thr Ser Tyr Ile Ala Thr Val Gln Ala Gln Leu Ala Thr Pro Leu 675 680 685 Met Lys Ile Ser Ala Gln Ala Cys Tyr Asn Glu Ser Ile His Glu Leu 690 695 700 Lys Thr Lys Tyr Arg Ser Phe Ser Lys Glu Gly Phe Gly Ser Trp His 705 710 715 720 Ser Val Ala Val Ser Gly Glu Val Cys Ala Ser Ile Pro Ile Val Ser 725 730 735 Asn Gly Ser Gly Leu Phe Ser Ser Phe Ser Ile Phe Ser Lys Leu Gln 740 745 750 Gly Phe Ser Gly Thr Gln Asp Gly Phe Glu Glu Ser Ser Gly Glu Ile 755 760 765 Arg Ser Phe Ser Ala Ser Ser Phe Arg Asn Ile Ser Leu Pro Met Gly 770 775 780 Ile Thr Phe Glu Lys Lys Ser Gln Lys Thr Arg Asn Tyr Tyr Tyr Phe 785 790 795 800 Leu Gly Ala Tyr Ile Gln Asp Leu Lys Arg Asp Val Glu Ser Gly Pro 805 810 815 Val Val Leu Leu Lys Asn Ala Val Ser Trp Asp Ala Pro Met Ala Asn 820 825 830 Leu Asp Ser Arg Ala Tyr Met Phe Arg Leu Thr Asn Gln Arg Ala Leu 835 840 845 His Arg Leu Gln Thr Leu Leu Asn Val Ser Tyr Val Leu Arg Gly Gln 850 855 860 Ser His Ser Tyr Ser Leu Asp Leu Gly Thr Thr Tyr Arg Phe 865 870 875 8 2637 DNA Chlamydia trachomatis 8 atgcgacctg atcatatgaa cttctgttgt ctatgtgctg ctattttgtc atccacagcg 60 gtcctctttg gccaggatcc cttaggtgaa accgccctcc tcactaaaaa tcctaatcat 120 gtcgtctgta cattttttga ggactgtacc atggagagcc tctttcctgc tctttgtgct 180 catgcatcac aagatgatcc tttgtatgta cttggaaatt cctactgttg gttcgtatct 240 aaactccata tcacggaccc caaagaggct ctttttaaag aaaaaggaga tctttccatt 300 caaaattttc gcttcctttc cttcacagat tgctcttcca aggaaagctc tccttctatt 360 attcatcaaa agaatggtca gttatccttg cgcaataatg gtagcatgag tttctgtcga 420 aatcatgctg aaggctctgg aggagccatc tctgcggatg ccttttctct acaacacaac 480 tatcttttca cagcttttga agagaattct tctaaaggaa atggcggagc cattcaggct 540 caaaccttct ctttatctag aaatgtgtcg cctatttctt tcgcccgtaa tcgtgcggat 600 ttaaatggcg gcgctatttg ctgtagtaat cttatttgtt cagggaatgt aaaccctctc 660 tttttcactg gaaactccgc cacgaatgga ggcgctattt gttgtatcag cgatctaaac 720 acctcagaaa aaggctctct ctctcttgct tgtaaccaag aaacgctatt tgcaagcaat 780 tctgctaaag aaaaaggcgg ggctatttat gccaagcaca tggtattgcg ttataacggt 840 cctgtttcct tcattaacaa cagcgctaaa ataggtggag ctatcgccat ccagtccgga 900 gggagtctct ctatccttgc aggtgaagga tctgttctgt tccagaataa ctcccaacgc 960 acctccgacc aaggtctagt aagaaacgcc atctacttag agaaagatgc gattctttct 1020 tccttagaag ctcgcaacgg agatattctt ttctttgatc ctattgtaca agaaagtagc 1080 agcaaagaat cgcctcttcc ctcctctttg caagccagcg tgacttctcc caccccagcc 1140 accgcatctc ctttagttat tcagacaagt gcaaaccgtt cagtgatttt ctcgagcgaa 1200 cgtctttctg aagaagaaaa aactcctgat aacctcactt cccaactaca gcagcctatc 1260 gaactgaaat ccggacgctt agttttaaaa gatcgcgctg tcctttccgc gccttctctc 1320 tctcaggatc ctcaagctct cctcattatg gaagcgggaa cttctttaaa aacttcctct 1380 gatttgaagt tagctacgct aagtattccc cttcattcct tagatactga aaaaagcgta 1440 actatccacg cccctaacct ttctatccaa aagatcttcc tctctaattc tggagatgag 1500 aatttttatg aaaatgtaga gcttctcagt aaagagcaaa acaatattcc tctccttact 1560 ctctctaaag agcaatctca tttacatctt cctgatggga acctctcttc tcactttgga 1620 tatcaaggag attggacttt ttcttggaaa gattctgatg aagggcattc tctgattgct 1680 aattggacgc ctaaaaacta tgtgcctcat ccagaacgtc aatctacact cgttgcgaac 1740 actctttgga acacctattc cgatatgcaa gctgtgcagt cgatgattaa tacaatagcg 1800 cacggaggag cctatctatt tggaacgtgg ggatctgctg tttctaattt attctatgct 1860 cacgacagct ctgggaaacc tatcgataat tggcatcata gaagccttgg ctacctattc 1920 ggtatcagta ctcacagttt agatgaccat tctttctgct tggctgcagg acaattactc 1980 gggaaatcgt ccgattcctt tattacgtct acagaaacga cctcctatat agctactgta 2040 caagcgcaac tcgctacccc tctaatgaaa atctctgcac aggcatgcta taatgaaagt 2100 atccatgagc taaaaacaaa atatcgctcc ttctctaaag aaggattcgg atcctggcat 2160 agcgttgcag tatccggaga agtgtgcgca tcgattccta ttgtatccaa tggttccgga 2220 ctgttcagct ccttctctat tttctctaaa ctgcaaggat tttcaggaac acaggacggt 2280 tttgaggaga gttcgggaga gattcggtcc ttttctgcca gctctttcag aaatatttca 2340 cttcctatgg gaataacatt tgaaaaaaaa tcccaaaaaa cacgaaacta ctattacttt 2400 ctgggagcct acatccaaga cctaaaacgt gatgtggaat cgggacctgt agtgttactc 2460 aaaaatgccg tctcctggga tgctcctatg gcgaacttgg attcgcgagc ctacatgttc 2520 aggcttacga atcaaagagc tctgcataga cttcagacgc tgttaaatgt gtcttacgta 2580 ctgcgcgggc aaagccatag ttactccctg gatctgggga ccacttacag gttctag 2637 9 1013 PRT Chlamydia trachomatis 9 Met Gln Thr Ser Phe His Lys Phe Phe Leu Ser Met Ile Leu Ala Tyr 1 5 10 15 Ser Cys Cys Ser Leu Ser Gly Gly Gly Tyr Ala Ala Glu Ile Met Ile 20 25 30 Pro Gln Gly Ile Tyr Asp Gly Glu Thr Leu Thr Val Ser Phe Pro Tyr 35 40 45 Thr Val Ile Gly Asp Pro Ser Gly Thr Thr Val Phe Ser Ala Gly Glu 50 55 60 Leu Thr Leu Lys Asn Leu Asp Asn Ser Ile Ala Ala Leu Pro Leu Ser 65 70 75 80 Cys Phe Gly Asn Leu Leu Gly Ser Phe Thr Val Leu Gly Arg Gly His 85 90 95 Ser Leu Thr Phe Glu Asn Ile Arg Thr Ser Thr Asn Gly Ala Ala Leu 100 105 110 Ser Asp Ser Ala Asn Ser Gly Leu Phe Thr Ile Glu Gly Phe Lys Glu 115 120 125 Leu Ser Phe Ser Asn Cys Asn Ser Leu Leu Ala Val Leu Pro Ala Ala 130 135 140 Thr Thr Asn Asn Gly Ser Gln Thr Pro Thr Thr Thr Ser Thr Pro Ser 145 150 155 160 Asn Gly Thr Ile Tyr Ser Lys Thr Asp Leu Leu Leu Leu Asn Asn Glu 165 170 175 Lys Phe Ser Phe Tyr Ser Asn Leu Val Ser Gly Asp Gly Gly Ala Ile 180 185 190 Asp Ala Lys Ser Leu Thr Val Gln Gly Ile Ser Lys Leu Cys Val Phe 195 200 205 Gln Glu Asn Thr Ala Gln Ala Asp Gly Gly Ala Cys Gln Val Val Thr 210 215 220 Ser Phe Ser Ala Met Ala Asn Glu Ala Pro Ile Ala Phe Ile Ala Asn 225 230 235 240 Val Ala Gly Val Arg Gly Gly Gly Ile Ala Ala Val Gln Asp Gly Gln 245 250 255 Gln Gly Val Ser Ser Ser Thr Ser Thr Glu Asp Pro Val Val Ser Phe 260 265 270 Ser Arg Asn Thr Ala Val Glu Phe Asp Gly Asn Val Ala Arg Val Gly 275 280 285 Gly Gly Ile Tyr Ser Tyr Gly Asn Val Ala Phe Leu Asn Asn Gly Lys 290 295 300 Thr Leu Phe Leu Asn Asn Val Ala Ser Pro Val Tyr Ile Ala Ala Glu 305 310 315 320 Gln Pro Thr Asn Gly Gln Ala Ser Asn Thr Ser Asp Asn Tyr Gly Asp 325 330 335 Gly Gly Ala Ile Phe Cys Lys Asn Gly Ala Gln Ala Ala Gly Ser Asn 340 345 350 Asn Ser Gly Ser Val Ser Phe Asp Gly Glu Gly Val Val Phe Phe Ser 355 360 365 Ser Asn Val Ala Ala Gly Lys Gly Gly Ala Ile Tyr Ala Lys Lys Leu 370 375 380 Ser Val Ala Asn Cys Gly Pro Val Gln Phe Leu Gly Asn Ile Ala Asn 385 390 395 400 Asp Gly Gly Ala Ile Tyr Leu Gly Glu Ser Gly Glu Leu Ser Leu Ser 405 410 415 Ala Asp Tyr Gly Asp Ile Ile Phe Asp Gly Asn Leu Lys Arg Thr Ala 420 425 430 Lys Glu Asn Ala Ala Asp Val Asn Gly Val Thr Val Ser Ser Gln Ala 435 440 445 Ile Ser Met Gly Ser Gly Gly Lys Ile Thr Thr Leu Arg Ala Lys Ala 450 455 460 Gly His Gln Ile Leu Phe Asn Asp Pro Ile Glu Met Ala Asn Gly Asn 465 470 475 480 Asn Gln Pro Ala Gln Ser Ser Glu Pro Leu Lys Ile Asn Asp Gly Glu 485 490 495 Gly Tyr Thr Gly Asp Ile Val Phe Ala Asn Gly Asn Ser Thr Leu Tyr 500 505 510 Gln Asn Val Thr Ile Glu Gln Gly Arg Ile Val Leu Arg Glu Lys Ala 515 520 525 Lys Leu Ser Val Asn Ser Leu Ser Gln Thr Gly Gly Ser Leu Tyr Met 530 535 540 Glu Ala Gly Ser Thr Leu Asp Phe Val Thr Pro Gln Pro Pro Gln Gln 545 550 555 560 Pro Pro Ala Ala Asn Gln Leu Ile Thr Leu Ser Asn Leu His Leu Ser 565 570 575 Leu Ser Ser Leu Leu Ala Asn Asn Ala Val Thr Asn Pro Pro Thr Asn 580 585 590 Pro Pro Ala Gln Asp Ser His Pro Ala Ile Ile Gly Ser Thr Thr Ala 595 600 605 Gly Ser Val Thr Ile Ser Gly Pro Ile Phe Phe Glu Asp Leu Asp Asp 610 615 620 Thr Ala Tyr Asp Arg Tyr Asp Trp Leu Gly Ser Asn Gln Lys Ile Asp 625 630 635 640 Val Leu Lys Leu Gln Leu Gly Thr Gln Pro Ser Ala Asn Ala Pro Ser 645 650 655 Asp Leu Thr Leu Gly Asn Glu Met Pro Lys Tyr Gly Tyr Gln Gly Ser 660 665 670 Trp Lys Leu Ala Trp Asp Pro Asn Thr Ala Asn Asn Gly Pro Tyr Thr 675 680 685 Leu Lys Ala Thr Trp Thr Lys Thr Gly Tyr Asn Pro Gly Pro Glu Arg 690 695 700 Val Ala Ser Leu Val Pro Asn Ser Leu Trp Gly Ser Ile Leu Asp Ile 705 710 715 720 Arg Ser Ala His Ser Ala Ile Gln Ala Ser Val Asp Gly Arg Ser Tyr 725 730 735 Cys Arg Gly Leu Trp Val Ser Gly Val Ser Asn Phe Phe Tyr His Asp 740 745 750 Arg Asp Ala Leu Gly Gln Gly Tyr Arg Tyr Ile Ser Gly Gly Tyr Ser 755 760 765 Leu Gly Ala Asn Ser Tyr Phe Gly Ser Ser Met Phe Gly Leu Ala Phe 770 775 780 Thr Glu Val Phe Gly Arg Ser Lys Asp Tyr Val Val Cys Arg Ser Asn 785 790 795 800 His His Ala Cys Ile Gly Ser Val Tyr Leu Ser Thr Lys Gln Ala Leu 805 810 815 Cys Gly Ser Tyr Leu Phe Gly Asp Ala Phe Ile Arg Ala Ser Tyr Gly 820 825 830 Phe Gly Asn Gln His Met Lys Thr Ser Tyr Thr Phe Ala Glu Glu Ser 835 840 845 Asp Val Arg Trp Asp Asn Asn Cys Leu Val Gly Glu Ile Gly Val Gly 850 855 860 Leu Pro Ile Val Ile Thr Pro Ser Lys Leu Tyr Leu Asn Glu Leu Arg 865 870 875 880 Pro Phe Val Gln Ala Glu Phe Ser Tyr Ala Asp His Glu Ser Phe Thr 885 890 895 Glu Glu Gly Asp Gln Ala Arg Ala Phe Arg Ser Gly His Leu Met Asn 900 905 910 Leu Ser Val Pro Val Gly Val Lys Phe Asp Arg Cys Ser Ser Thr His 915 920 925 Pro Asn Lys Tyr Ser Phe Met Gly Ala Tyr Ile Cys Asp Ala Tyr Arg 930 935 940 Thr Ile Ser Gly Thr Gln Thr Thr Leu Leu Ser His Gln Glu Thr Trp 945 950 955 960 Thr Thr Asp Ala Phe His Leu Ala Arg His Gly Val Ile Val Arg Gly 965 970 975 Ser Met Tyr Ala Ser Leu Thr Ser Asn Ile Glu Val Tyr Gly His Gly 980 985 990 Arg Tyr Glu Tyr Arg Asp Thr Ser Arg Gly Tyr Gly Leu Ser Ala Gly 995 1000 1005 Ser Lys Val Arg Phe 1010 10 3042 DNA Chlamydia trachomatis 10 atgcaaacgt ctttccataa gttctttctt tcaatgattc tagcttattc ttgctgctct 60 ttaagtgggg gggggtatgc agcagaaatc atgattcctc aaggaattta cgatggggag 120 acgttaactg tatcatttcc ctatactgtt ataggagatc cgagtgggac tactgttttt 180 tctgcaggag agttaacgtt aaaaaatctt gacaattcta ttgcagcttt gcctttaagt 240 tgttttggga acttattagg gagttttact gttttaggga gaggacactc gttgactttc 300 gagaacatac ggacttctac aaatggagct gcactaagtg acagcgctaa tagcgggtta 360 tttactattg agggttttaa agaattatct ttttccaatt gcaactcatt acttgccgta 420 ctgcctgctg caacgactaa taatggtagc cagactccga cgacaacatc tacaccgtct 480 aatggtacta tttattctaa aacagatctt ttgttactca ataatgagaa gttctcattc 540 tatagtaatt tagtctctgg agatggggga gctatagatg ctaagagctt aacggttcaa 600 ggaattagca agctttgtgt cttccaagaa aatactgctc aagctgatgg gggagcttgt 660 caagtagtca ccagtttctc tgctatggct aacgaggctc ctattgcctt tatagcgaat 720 gttgcaggag taagaggggg agggattgct gctgttcagg atgggcagca gggagtgtca 780 tcatctactt caacagaaga tccagtagta agtttttcca gaaatactgc ggtagagttt 840 gatgggaacg tagcccgagt aggaggaggg atttactcct acgggaacgt tgctttcctg 900 aataatggaa aaaccttgtt tctcaacaat gttgcttctc ctgtttacat tgctgctgag 960 caaccaacaa atggacaggc ttctaatacg agtgataatt acggagatgg aggagctatc 1020 ttctgtaaga atggtgcgca agcagcagga tccaataact ctggatcagt ttcctttgat 1080 ggagagggag tagttttctt tagtagcaat gtagctgctg ggaaaggggg agctatttat 1140 gccaaaaagc tctcggttgc taactgtggc cctgtacaat tcttagggaa tatcgctaat 1200 gatggtggag cgatttattt aggagaatct ggagagctca gtttatctgc tgattatgga 1260 gatattattt tcgatgggaa tcttaaaaga acagccaaag agaatgctgc cgatgttaat 1320 ggcgtaactg tgtcctcaca agccatttcg atgggatcgg gagggaaaat aacgacatta 1380 agagctaaag cagggcatca gattctcttt aatgatccca tcgagatggc aaacggaaat 1440 aaccagccag cgcagtcttc cgaacctcta aaaattaacg atggtgaagg atacacaggg 1500 gatattgttt ttgctaatgg aaacagtact ttgtaccaaa atgttacgat agagcaagga 1560 aggattgttc ttcgtgaaaa ggcaaaatta tcagtgaatt ctctaagtca gacaggtggg 1620 agtctgtata tggaagctgg gagtacattg gattttgtaa ctccacaacc accacaacag 1680 cctcctgccg ctaatcagtt gatcacgctt tccaatctgc atttgtctct ttcttctttg 1740 ttagcaaaca atgcagttac gaatcctcct accaatcctc cagcgcaaga ttctcatcct 1800 gcaatcattg gtagcacaac tgctggttct gttacaatta gtgggcctat cttttttgag 1860 gatttggatg atacagctta tgataggtat gattggctag gttctaatca aaaaatcgat 1920 gtcctgaaat tacagttagg gactcagccc tcagctaatg ccccatcaga tttgactcta 1980 gggaatgaga tgcctaagta tggctatcaa ggaagctgga agcttgcgtg ggatcctaat 2040 acagcaaata atggtcctta tactctgaaa gctacatgga ctaaaactgg gtataatcct 2100 gggcctgagc gagtagcttc tttggttcca aatagtttat ggggatccat tttagatata 2160 cgatctgcgc attcagcaat tcaagcaagt gtggatgggc gctcttattg tcgaggatta 2220 tgggtttctg gagtttcgaa tttcttctat catgaccgcg atgctttagg tcagggatat 2280 cggtatatta gtgggggtta ttccttagga gcaaactcct actttggatc atcgatgttt 2340 ggtctagcat ttaccgaagt atttggtaga tctaaagatt atgtagtgtg tcgttccaat 2400 catcatgctt gcataggatc cgtttatcta tctaccaaac aagctttatg tggatcctat 2460 ttgttcggag atgcgtttat ccgtgctagc tacgggtttg ggaaccagca tatgaaaacc 2520 tcatacacat ttgcagagga gagcgatgtt cgttgggata ataactgtct ggttggagag 2580 attggagtgg gattaccgat tgtgattact ccatctaagc tctatttgaa tgagttgcgt 2640 cctttcgtgc aagctgagtt ttcttatgcc gatcatgaat cttttacaga ggaaggcgat 2700 caagctcggg cattcaggag tggacatctc atgaatctat cagttcctgt tggagtaaaa 2760 tttgatcgat gttctagtac acaccctaat aaatatagct ttatgggggc ttatatctgt 2820 gatgcttatc gcaccatctc tgggactcag acaacactcc tatcccatca agagacatgg 2880 acaacagatg cctttcattt ggcaagacat ggagtcatag ttagagggtc tatgtatgct 2940 tctctaacaa gcaatataga agtatatggc catggaagat atgagtatcg agatacttct 3000 cgaggttatg gtttgagtgc aggaagtaaa gtccggttct aa 3042 11 1034 PRT Chlamydia trachomatis 11 Met Ile Lys Arg Thr Ser Leu Ser Phe Ala Cys Leu Ser Phe Phe Tyr 1 5 10 15 Leu Ser Thr Ile Ser Ile Leu Gln Ala Asn Glu Thr Asp Thr Leu Gln 20 25 30 Phe Arg Arg Phe Thr Phe Ser Asp Arg Glu Ile Gln Phe Val Leu Asp 35 40 45 Pro Ala Ser Leu Ile Thr Ala Gln Asn Ile Val Leu Ser Asn Leu Gln 50 55 60 Ser Asn Gly Thr Gly Ala Cys Thr Ile Ser Gly Asn Thr Gln Thr Gln 65 70 75 80 Ile Phe Ser Asn Ser Val Asn Thr Thr Ala Asp Ser Gly Gly Ala Phe 85 90 95 Asp Met Val Thr Thr Ser Phe Thr Ala Ser Asp Asn Ala Asn Leu Leu 100 105 110 Phe Cys Asn Asn Tyr Cys Thr His Asn Lys Gly Gly Gly Ala Ile Arg 115 120 125 Ser Gly Gly Pro Ile Arg Phe Leu Asn Asn Gln Asp Val Leu Phe Tyr 130 135 140 Asn Asn Ile Ser Ala Gly Ala Lys Tyr Val Gly Thr Gly Asp His Asn 145 150 155 160 Glu Lys Asn Arg Gly Gly Ala Leu Tyr Ala Thr Thr Ile Thr Leu Thr 165 170 175 Gly Asn Arg Thr Leu Ala Phe Ile Asn Asn Met Ser Gly Asp Cys Gly 180 185 190 Gly Ala Ile Ser Ala Asp Thr Gln Ile Ser Ile Thr Asp Thr Val Lys 195 200 205 Gly Ile Leu Phe Glu Asn Asn His Thr Leu Asn His Ile Pro Tyr Thr 210 215 220 Gln Ala Glu Asn Met Ala Arg Gly Gly Ala Ile Cys Ser Arg Arg Asp 225 230 235 240 Leu Cys Ser Ile Ser Asn Asn Ser Gly Pro Ile Val Phe Asn Tyr Asn 245 250 255 Gln Gly Gly Lys Gly Gly Ala Ile Ser Ala Thr Arg Cys Val Ile Asp 260 265 270 Asn Asn Lys Glu Arg Ile Ile Phe Ser Asn Asn Ser Ser Leu Gly Trp 275 280 285 Ser Gln Ser Ser Ser Ala Ser Asn Gly Gly Ala Ile Gln Thr Thr Gln 290 295 300 Gly Phe Thr Leu Arg Asn Asn Lys Gly Ser Ile Tyr Phe Asp Ser Asn 305 310 315 320 Thr Ala Thr His Ala Gly Gly Ala Ile Asn Cys Gly Tyr Ile Asp Ile 325 330 335 Arg Asp Asn Gly Pro Val Tyr Phe Leu Asn Asn Ser Ala Ala Trp Gly 340 345 350 Ala Ala Phe Asn Leu Ser Lys Pro Arg Ser Ala Thr Asn Tyr Ile His 355 360 365 Thr Gly Thr Gly Asp Ile Val Phe Asn Asn Asn Val Val Phe Thr Leu 370 375 380 Asp Gly Asn Leu Leu Gly Lys Arg Lys Leu Phe His Ile Asn Asn Asn 385 390 395 400 Glu Ile Thr Pro Tyr Thr Leu Ser Leu Gly Ala Lys Lys Asp Thr Arg 405 410 415 Ile Tyr Phe Tyr Asp Leu Phe Gln Trp Glu Arg Val Lys Glu Asn Thr 420 425 430 Ser Asn Asn Pro Pro Ser Pro Thr Ser Arg Asn Thr Ile Thr Val Asn 435 440 445 Pro Glu Thr Glu Phe Ser Gly Ala Val Val Phe Ser Tyr Asn Gln Met 450 455 460 Ser Ser Asp Ile Arg Thr Leu Met Gly Lys Glu His Asn Tyr Ile Lys 465 470 475 480 Glu Ala Pro Thr Thr Leu Lys Phe Gly Thr Leu Ala Ile Glu Asp Asp 485 490 495 Ala Glu Leu Glu Ile Phe Asn Ile Pro Phe Thr Gln Asn Pro Thr Ser 500 505 510 Leu Leu Ala Leu Gly Ser Gly Ala Thr Leu Thr Val Gly Lys His Gly 515 520 525 Lys Leu Asn Ile Thr Asn Leu Gly Val Ile Leu Pro Ile Ile Leu Lys 530 535 540 Glu Gly Lys Ser Pro Pro Cys Ile Arg Val Asn Pro Gln Asp Met Thr 545 550 555 560 Gln Asn Thr Gly Thr Gly Gln Thr Pro Ser Ser Thr Ser Ser Ile Ser 565 570 575 Thr Pro Met Ile Ile Phe Asn Gly Arg Leu Ser Ile Val Asp Glu Asn 580 585 590 Tyr Glu Ser Val Tyr Asp Ser Met Asp Leu Ser Arg Gly Lys Ala Glu 595 600 605 Gln Leu Ile Leu Ser Ile Glu Thr Thr Asn Asp Gly Gln Leu Asp Ser 610 615 620 Asn Trp Gln Ser Ser Leu Asn Thr Ser Leu Leu Ser Pro Pro His Tyr 625 630 635 640 Gly Tyr Gln Gly Leu Trp Thr Pro Asn Trp Ile Thr Thr Thr Tyr Thr 645 650 655 Ile Thr Leu Asn Asn Asn Ser Ser Ala Pro Thr Ser Ala Thr Ser Ile 660 665 670 Ala Glu Gln Lys Lys Thr Ser Glu Thr Phe Thr Pro Ser Asn Thr Thr 675 680 685 Thr Ala Ser Ile Pro Asn Ile Lys Ala Ser Ala Gly Ser Gly Ser Gly 690 695 700 Ser Ala Ser Asn Ser Gly Glu Val Thr Ile Thr Lys His Thr Leu Val 705 710 715 720 Val Asn Trp Ala Pro Val Gly Tyr Ile Val Asp Pro Ile Arg Arg Gly 725 730 735 Asp Leu Ile Ala Asn Ser Leu Val His Ser Gly Arg Asn Met Thr Met 740 745 750 Gly Leu Arg Ser Leu Leu Pro Asp Asn Ser Trp Phe Ala Leu Gln Gly 755 760 765 Ala Ala Thr Thr Leu Phe Thr Lys Gln Gln Lys Arg Leu Ser Tyr His 770 775 780 Gly Tyr Ser Ser Ala Ser Lys Gly Tyr Thr Val Ser Ser Gln Ala Ser 785 790 795 800 Gly Ala His Gly His Lys Phe Leu Leu Ser Phe Ser Gln Ser Ser Asp 805 810 815 Lys Met Lys Glu Lys Glu Thr Asn Asn Arg Leu Ser Ser Arg Tyr Tyr 820 825 830 Leu Ser Ala Leu Cys Phe Glu His Pro Met Phe Asp Arg Ile Ala Leu 835 840 845 Ile Gly Ala Ala Ala Cys Asn Tyr Gly Thr His Asn Met Arg Ser Phe 850 855 860 Tyr Gly Thr Lys Lys Ser Ser Lys Gly Lys Phe His Ser Thr Thr Leu 865 870 875 880 Gly Ala Ser Leu Arg Cys Glu Leu Arg Asp Ser Met Pro Leu Arg Ser 885 890 895 Ile Met Leu Thr Pro Phe Ala Gln Ala Leu Phe Ser Arg Thr Glu Pro 900 905 910 Ala Ser Ile Arg Glu Ser Gly Asp Leu Ala Arg Leu Phe Thr Leu Glu 915 920 925 Gln Ala His Thr Ala Val Val Ser Pro Ile Gly Ile Lys Gly Ala Tyr 930 935 940 Ser Ser Asp Thr Trp Pro Thr Leu Ser Trp Glu Met Glu Leu Ala Tyr 945 950 955 960 Gln Pro Thr Leu Tyr Trp Lys Arg Pro Leu Leu Asn Thr Leu Leu Ile 965 970 975 Gln Asn Asn Gly Ser Trp Val Thr Thr Asn Thr Pro Leu Ala Lys His 980 985 990 Ser Phe Tyr Gly Arg Gly Ser His Ser Leu Lys Phe Ser His Leu Lys 995 1000 1005 Leu Phe Ala Asn Tyr Gln Ala Glu Val Ala Thr Ser Thr Val Ser His 1010 1015 1020 Tyr Ile Asn Ala Gly Gly Ala Leu Val Phe 1025 1030 12 3105 DNA Chlamydia trachomatis 12 atgattaaaa gaacttctct atcctttgct tgcctcagtt ttttttatct ttcaactata 60 tccattttgc aagctaatga aacggatacg ctacagttcc ggcgatttac tttttcggat 120 agagagattc agttcgtcct agatcccgcc tctttaatta ccgcccaaaa catcgtttta 180 tctaatttac agtcaaacgg aaccggagcc tgtaccattt caggcaatac gcaaactcaa 240 atcttttcta attccgttaa caccaccgca gattctggtg gagcctttga tatggttact 300 acctcattca cggcctctga taatgctaat ctactcttct gcaacaacta ctgcacacat 360 aataaaggcg gaggagctat tcgttccgga ggacctattc gattcttaaa taatcaagac 420 gtgctttttt ataataacat atcggcaggg gctaaatatg ttggaacagg agatcacaac 480 gaaaaaaata ggggcggtgc gctttatgca actactatca ctttgacagg gaatcgaact 540 cttgccttta ttaacaatat gtctggagac tgcggtggag ccatctctgc tgacactcaa 600 atatcaataa ctgataccgt taaaggaatt ttatttgaaa acaatcacac gctcaatcat 660 ataccgtaca cgcaagctga aaatatggca cgaggaggag caatctgtag tagaagagac 720 ttgtgctcaa tcagcaataa ttctggtccc atagttttta actataacca aggcgggaaa 780 ggtggagcta ttagcgctac ccgatgtgtt attgacaata acaaagaaag aatcatcttt 840 tcaaacaata gttccctggg atggagccaa tcttcttctg caagtaacgg aggagccatt 900 caaacgacac aaggatttac tttacgaaat aataaaggct ctatctactt cgacagcaac 960 actgctacac acgccggggg agccattaac tgtggttaca ttgacatccg agataacgga 1020 cccgtctatt ttctaaataa ctctgctgcc tggggagcgg cctttaattt atcgaaacca 1080 cgttcagcga caaattatat ccatacaggg acaggcgata ttgtttttaa taataacgtt 1140 gtctttactc ttgacggtaa tttattaggg aaacggaaac tttttcatat taataataat 1200 gagataacac catatacatt gtctctcggc gctaaaaaag atactcgtat ctatttttat 1260 gatcttttcc aatgggagcg tgttaaagaa aatactagca ataacccacc atctcctacc 1320 agtagaaaca ccattaccgt taacccggaa acagagtttt ctggagctgt tgtgttctcc 1380 tacaatcaaa tgtctagtga catacgaact ctgatgggta aagaacacaa ttacattaaa 1440 gaagccccaa ctactttaaa attcggaacg ctagccatag aagatgatgc agaattagaa 1500 atcttcaata tcccgtttac ccaaaatccg actagccttc ttgctttagg aagcggcgct 1560 acgctgactg ttggaaagca cggtaagctc aatattacaa atcttggtgt tattttaccc 1620 attattctca aagaggggaa gagtccgcct tgtattcgcg tcaacccaca agatatgacc 1680 caaaatactg gtaccggcca aactccatca agcacaagta gtataagcac tccaatgatt 1740 atctttaatg ggcgcctctc aattgtagac gaaaattatg aatcagtcta cgacagtatg 1800 gacctctcca gagggaaagc agaacaacta attctatcca tagaaaccac taatgatggg 1860 caattagact ccaattggca aagttctctg aatacttctc tactctctcc tccacactat 1920 ggctatcaag gtctatggac tcctaattgg ataacaacaa cctataccat cacgcttaat 1980 aataattctt cagctccaac atctgctacc tccatcgctg agcagaaaaa aactagtgaa 2040 acttttactc ctagtaacac aactacagct agtatcccta atattaaagc ttccgcagga 2100 tcaggctctg gatcggcttc caattcagga gaagttacga ttaccaaaca tacccttgtt 2160 gtaaactggg caccagtcgg ctacatagta gatcctattc gtagaggaga tctgatagcc 2220 aatagcttag tacattcagg aagaaacatg accatgggct tacgatcatt actcccggat 2280 aactcttggt ttgctttgca aggagctgca acaacattat ttacaaaaca acaaaaacgt 2340 ttgagttatc atggctactc ttctgcatca aaggggtata ccgtctcttc tcaagcatca 2400 ggagctcatg gtcataagtt tcttctttcc ttctcccagt catctgataa gatgaaagaa 2460 aaagaaacaa ataaccgcct ttcttctcgt tactatcttt ctgctttatg tttcgaacat 2520 cctatgtttg atcgcattgc tcttatcgga gcagcagctt gcaattatgg aacacataac 2580 atgcggagtt tctatggaac taaaaaatct tctaaaggga aatttcactc tacaacctta 2640 ggagcttctc ttcgctgtga actacgcgat agtatgcctt tacgatcaat aatgctcacc 2700 ccatttgctc aggctttatt ctctcgaaca gaaccagctt ctatccgaga aagcggtgat 2760 ctagctagat tatttacatt agagcaagcc catactgccg ttgtctctcc aataggaatc 2820 aaaggagctt attcttctga tacatggcca acactctctt gggaaatgga actagcttac 2880 caacccaccc tctactggaa acgtcctcta ctcaacacac tattaatcca aaataacggt 2940 tcttgggtca ccacaaatac cccattagct aaacattcct tttatgggag aggttctcac 3000 tccctcaaat tttctcatct gaaactattt gctaactatc aagcagaagt ggctacttcc 3060 actgtctcac actacatcaa tgcaggagga gctctggtct tttaa 3105 13 964 PRT Chlamydia trachomatis 13 Met Lys Lys Ala Phe Phe Phe Phe Leu Ile Gly Asn Ser Leu Ser Gly 1 5 10 15 Leu Ala Arg Glu Val Pro Ser Arg Ile Phe Leu Met Pro Asn Ser Val 20 25 30 Pro Asp Pro Thr Lys Glu Ser Leu Ser Asn Lys Ile Ser Leu Thr Gly 35 40 45 Asp Thr His Asn Leu Thr Asn Cys Tyr Leu Asp Asn Leu Arg Tyr Ile 50 55 60 Leu Ala Ile Leu Gln Lys Thr Pro Asn Glu Gly Ala Ala Val Thr Ile 65 70 75 80 Thr Asp Tyr Leu Ser Phe Phe Asp Thr Gln Lys Glu Gly Ile Tyr Phe 85 90 95 Ala Lys Asn Leu Thr Pro Glu Ser Gly Gly Ala Ile Gly Tyr Ala Ser 100 105 110 Pro Asn Ser Pro Thr Val Glu Ile Arg Asp Thr Ile Gly Pro Val Ile 115 120 125 Phe Glu Asn Asn Thr Cys Cys Arg Leu Phe Thr Trp Arg Asn Pro Tyr 130 135 140 Ala Ala Asp Lys Ile Arg Glu Gly Gly Ala Ile His Ala Gln Asn Leu 145 150 155 160 Tyr Ile Asn His Asn His Asp Val Val Gly Phe Met Lys Asn Phe Ser 165 170 175 Tyr Val Gln Gly Gly Ala Ile Ser Thr Ala Asn Thr Phe Val Val Ser 180 185 190 Glu Asn Gln Ser Cys Phe Leu Phe Met Asp Asn Ile Cys Ile Gln Thr 195 200 205 Asn Thr Ala Gly Lys Gly Gly Ala Ile Tyr Ala Gly Thr Ser Asn Ser 210 215 220 Phe Glu Ser Asn Asn Cys Asp Leu Phe Phe Ile Asn Asn Ala Cys Cys 225 230 235 240 Ala Gly Gly Ala Ile Phe Ser Pro Ile Cys Ser Leu Thr Gly Asn Arg 245 250 255 Gly Asn Ile Val Phe Tyr Asn Asn Arg Cys Phe Lys Asn Val Glu Thr 260 265 270 Ala Ser Ser Glu Ala Ser Asp Gly Gly Ala Ile Lys Val Thr Thr Arg 275 280 285 Leu Asp Val Thr Gly Asn Arg Gly Arg Ile Phe Phe Ser Asp Asn Ile 290 295 300 Thr Lys Asn Tyr Gly Gly Ala Ile Tyr Ala Pro Val Val Thr Leu Val 305 310 315 320 Asp Asn Gly Pro Thr Tyr Phe Ile Asn Asn Ile Ala Asn Asn Lys Gly 325 330 335 Gly Ala Ile Tyr Ile Asp Gly Thr Ser Asn Ser Lys Ile Ser Ala Asp 340 345 350 Arg His Ala Ile Ile Phe Asn Glu Asn Ile Val Thr Asn Val Thr Asn 355 360 365 Ala Asn Gly Thr Ser Thr Ser Ala Asn Pro Pro Arg Arg Asn Ala Ile 370 375 380 Thr Val Ala Ser Ser Ser Gly Glu Ile Leu Leu Gly Ala Gly Ser Ser 385 390 395 400 Gln Asn Leu Ile Phe Tyr Asp Pro Ile Glu Val Ser Asn Ala Gly Val 405 410 415 Ser Val Ser Phe Asn Lys Glu Ala Asp Gln Thr Gly Ser Val Val Phe 420 425 430 Ser Gly Ala Thr Val Asn Ser Ala Asp Phe His Gln Arg Asn Leu Gln 435 440 445 Thr Lys Thr Pro Ala Pro Leu Thr Leu Ser Asn Gly Phe Leu Cys Ile 450 455 460 Glu Asp His Ala Gln Leu Thr Val Asn Arg Phe Thr Gln Thr Gly Gly 465 470 475 480 Val Val Ser Leu Gly Asn Gly Ala Val Leu Ser Cys Tyr Lys Asn Gly 485 490 495 Thr Gly Asp Ser Ala Ser Asn Ala Ser Ile Thr Leu Lys His Ile Gly 500 505 510 Leu Asn Leu Ser Ser Ile Leu Lys Ser Gly Ala Glu Ile Pro Leu Leu 515 520 525 Trp Val Glu Pro Thr Asn Asn Ser Asn Asn Tyr Thr Ala Asp Thr Ala 530 535 540 Ala Thr Phe Ser Leu Ser Asp Val Lys Leu Ser Leu Ile Asp Asp Tyr 545 550 555 560 Gly Asn Ser Pro Tyr Glu Ser Thr Asp Leu Thr His Ala Leu Ser Ser 565 570 575 Gln Pro Met Leu Ser Ile Ser Glu Ala Ser Asp Asn Gln Leu Gln Ser 580 585 590 Glu Asn Ile Asp Phe Ser Gly Leu Asn Val Pro His Tyr Gly Trp Gln 595 600 605 Gly Leu Trp Thr Trp Gly Trp Ala Lys Thr Gln Asp Pro Glu Pro Ala 610 615 620 Ser Ser Ala Thr Ile Thr Asp Pro Gln Lys Ala Asn Arg Phe His Arg 625 630 635 640 Thr Leu Leu Leu Thr Trp Leu Pro Ala Gly Tyr Val Pro Ser Pro Lys 645 650 655 His Arg Ser Pro Leu Ile Ala Asn Thr Leu Trp Gly Asn Met Leu Leu 660 665 670 Ala Thr Glu Ser Leu Lys Asn Ser Ala Glu Leu Thr Pro Ser Gly His 675 680 685 Pro Phe Trp Gly Ile Thr Gly Gly Gly Leu Gly Met Met Val Tyr Gln 690 695 700 Asp Pro Arg Glu Asn His Pro Gly Phe His Met Arg Ser Ser Gly Tyr 705 710 715 720 Ser Ala Gly Met Ile Ala Gly Gln Thr His Thr Phe Ser Leu Lys Phe 725 730 735 Ser Gln Thr Tyr Thr Lys Leu Asn Glu Arg Tyr Ala Lys Asn Asn Val 740 745 750 Ser Ser Lys Asn Tyr Ser Cys Gln Gly Glu Met Leu Phe Ser Leu Gln 755 760 765 Glu Gly Phe Leu Leu Thr Lys Leu Val Gly Leu Tyr Ser Tyr Gly Asp 770 775 780 His Asn Cys His His Phe Tyr Thr Gln Gly Glu Asn Leu Thr Ser Gln 785 790 795 800 Gly Thr Phe Arg Ser Gln Thr Met Gly Gly Ala Val Phe Phe Asp Leu 805 810 815 Pro Met Lys Pro Phe Gly Ser Thr His Ile Leu Thr Ala Pro Phe Leu 820 825 830 Gly Ala Leu Gly Ile Tyr Ser Ser Leu Ser His Phe Thr Glu Val Gly 835 840 845 Ala Tyr Pro Arg Ser Phe Ser Thr Lys Thr Pro Leu Ile Asn Val Leu 850 855 860 Val Pro Ile Gly Val Lys Gly Ser Phe Met Asn Ala Thr His Arg Pro 865 870 875 880 Gln Ala Trp Thr Val Glu Leu Ala Tyr Gln Pro Val Leu Tyr Arg Gln 885 890 895 Glu Pro Gly Ile Ala Ala Gln Leu Leu Ala Ser Lys Gly Ile Trp Phe 900 905 910 Gly Ser Gly Ser Pro Ser Ser Arg His Ala Met Ser Tyr Lys Ile Ser 915 920 925 Gln Gln Thr Gln Pro Leu Ser Trp Leu Thr Leu His Phe Gln Tyr His 930 935 940 Gly Phe Tyr Ser Ser Ser Thr Phe Cys Asn Tyr Leu Asn Gly Glu Ile 945 950 955 960 Ala Leu Arg Phe 14 2895 DNA Chlamydia trachomatis 14 atgaaaaaag cgtttttctt tttccttatc ggaaactccc tatcaggact agctagagag 60 gttccttcta gaatctttct tatgcccaac tcagttccag atcctacgaa agagtcgcta 120 tcaaataaaa ttagtttgac aggagacact cacaatctca ctaactgcta tctcgataac 180 ctacgctaca tactggctat tctacaaaaa actcccaatg aaggagctgc tgtcacaata 240 acagattacc taagcttttt tgatacacaa aaagaaggta tttattttgc aaaaaatctc 300 acccctgaaa gtggtggtgc gattggttat gcgagtccca attctcctac cgtggagatt 360 cgtgatacaa taggtcctgt aatctttgaa aataatactt gttgcagact atttacatgg 420 agaaatcctt atgctgctga taaaataaga gaaggcggag ccattcatgc tcaaaatctt 480 tacataaatc ataatcatga tgtggtcgga tttatgaaga acttttctta tgtccaagga 540 ggagccatta gtaccgctaa tacctttgtt gtgagcgaga atcagtcttg ttttctcttt 600 atggacaaca tctgtattca aactaataca gcaggaaaag gtggcgctat ctatgctgga 660 acgagcaatt cttttgagag taataactgc gatctcttct tcatcaataa cgcctgttgt 720 gcaggaggag cgatcttctc ccctatctgt tctctaacag gaaatcgtgg taacatcgtt 780 ttctataaca atcgctgctt taaaaatgta gaaacagctt cttcagaagc ttctgatgga 840 ggagcaatta aagtaactac tcgcctagat gttacaggca atcgtggtag gatctttttt 900 agtgacaata tcacaaaaaa ttatggcgga gctatttacg ctcctgtagt taccctagtg 960 gataatggcc ctacctactt tataaacaat atcgccaata ataagggggg cgctatctat 1020 atagacggaa ccagtaactc caaaatttct gccgaccgcc atgctattat ttttaatgaa 1080 aatattgtga ctaatgtaac taatgcaaat ggtaccagta cgtcagctaa tcctcctaga 1140 agaaatgcaa taacagtagc aagctcctct ggtgaaattc tattaggagc agggagtagc 1200 caaaatttaa ttttttatga tcctattgaa gttagcaatg caggggtctc tgtgtccttc 1260 aataaggaag ctgatcaaac aggctctgta gtattttcag gagctactgt taattctgca 1320 gattttcatc aacgcaattt acaaacaaaa acacctgcac cccttactct cagtaatggt 1380 tttctatgta tcgaagatca tgctcagctt acagtgaatc gattcacaca aactgggggt 1440 gttgtttctc ttgggaatgg agcagttctg agttgctata aaaatggtac aggagattct 1500 gctagcaatg cctctataac actgaagcat attggattga atctttcttc cattctgaaa 1560 agtggtgctg agattccttt attgtgggta gagcctacaa ataacagcaa taactataca 1620 gcagatactg cagctacctt ttcattaagt gatgtaaaac tctcactcat tgatgactac 1680 gggaactctc cttatgaatc cacagatctg acccatgctc tgtcatcaca gcctatgcta 1740 tctatttctg aagctagcga taaccagcta caatcagaaa atatagattt ttcgggacta 1800 aatgtccctc attatggatg gcaaggactt tggacttggg gctgggcaaa aactcaagat 1860 ccagaaccag catcttcagc aacaatcact gatccacaaa aagccaatag atttcataga 1920 accttactac taacatggct tcctgccggg tatgttccta gcccaaaaca cagaagtccc 1980 ctcatagcta acaccttatg ggggaatatg ctgcttgcaa cagaaagctt aaaaaatagt 2040 gcagagctga cacctagtgg tcatcctttc tggggaatta caggaggagg actaggcatg 2100 atggtttacc aagatcctcg agaaaatcat cctggattcc atatgcgctc ttccggatac 2160 tctgcgggga tgatagcagg gcagacacac accttctcat tgaaattcag tcagacctac 2220 accaaactca atgagcgtta cgcaaaaaac aacgtatctt ctaaaaatta ctcatgccaa 2280 ggagaaatgc tcttctcatt gcaagaaggt ttcttgctga ctaaattagt tgggctttac 2340 agctatggag accataactg tcaccatttc tatactcaag gagaaaatct aacatctcaa 2400 gggacgttcc gcagtcaaac gatgggaggt gctgtctttt ttgatctccc tatgaaaccc 2460 tttggatcaa cgcatatact gacagctccc tttttaggtg ctcttggtat ttattctagc 2520 ctgtctcact ttactgaggt gggagcctat ccgcgaagct tttctacaaa gactcctttg 2580 atcaatgtcc tagtccctat tggagttaaa ggtagcttta tgaatgctac ccacagacct 2640 caagcctgga ctgtagaatt ggcataccaa cccgttctgt atagacaaga accagggatc 2700 gcagcccagc tcctagccag taagggtatt tggttcggta gtggaagccc ctcatcgcgt 2760 catgccatgt cctataaaat ctcacagcaa acacaacctt tgagttggtt aactctccat 2820 ttccagtatc atggattcta ctcctcttca accttctgta attatctcaa tggggaaatt 2880 gctctgcgat tctag 2895 15 1531 PRT Chlamydia trachomatis 15 Met Ser Ser Glu Lys Asp Ile Lys Ser Thr Cys Ser Lys Phe Ser Leu 1 5 10 15 Ser Val Val Ala Ala Ile Leu Ala Ser Val Ser Gly Leu Ala Ser Cys 20 25 30 Val Asp Leu His Ala Gly Gly Gln Ser Val Asn Glu Leu Val Tyr Val 35 40 45 Gly Pro Gln Ala Val Leu Leu Leu Asp Gln Ile Arg Asp Leu Phe Val 50 55 60 Gly Ser Lys Asp Ser Gln Ala Glu Gly Gln Tyr Arg Leu Ile Val Gly 65 70 75 80 Asp Pro Ser Ser Phe Gln Glu Lys Asp Ala Asp Thr Leu Pro Gly Lys 85 90 95 Val Glu Gln Ser Thr Leu Phe Ser Val Thr Asn Pro Val Val Phe Gln 100 105 110 Gly Val Asp Gln Gln Asp Gln Val Ser Ser Gln Gly Leu Ile Cys Ser 115 120 125 Phe Thr Ser Ser Asn Leu Asp Ser Pro Arg Asp Gly Glu Ser Phe Leu 130 135 140 Gly Ile Ala Phe Val Gly Asp Ser Ser Lys Ala Gly Ile Thr Leu Thr 145 150 155 160 Asp Val Lys Ala Ser Leu Ser Gly Ala Ala Leu Tyr Ser Thr Glu Asp 165 170 175 Leu Ile Phe Glu Lys Ile Lys Gly Gly Leu Glu Phe Ala Ser Cys Ser 180 185 190 Ser Leu Glu Gln Gly Gly Ala Cys Ala Ala Gln Ser Ile Leu Ile His 195 200 205 Asp Cys Gln Gly Leu Gln Val Lys His Cys Thr Thr Ala Val Asn Ala 210 215 220 Glu Gly Ser Ser Ala Asn Asp His Leu Gly Phe Gly Gly Gly Ala Phe 225 230 235 240 Phe Val Thr Gly Ser Leu Ser Gly Glu Lys Ser Leu Tyr Met Pro Ala 245 250 255 Gly Asp Met Val Val Ala Asn Cys Asp Gly Ala Ile Ser Phe Glu Gly 260 265 270 Asn Ser Ala Asn Phe Ala Asn Gly Gly Ala Ile Ala Ala Ser Gly Lys 275 280 285 Val Leu Phe Val Ala Asn Asp Lys Lys Thr Ser Phe Ile Glu Asn Arg 290 295 300 Ala Leu Ser Gly Gly Ala Ile Ala Ala Ser Ser Asp Ile Ala Phe Gln 305 310 315 320 Asn Cys Ala Glu Leu Val Phe Lys Gly Asn Cys Ala Ile Gly Thr Glu 325 330 335 Asp Lys Gly Ser Leu Gly Gly Gly Ala Ile Ser Ser Leu Gly Thr Val 340 345 350 Leu Leu Gln Gly Asn His Gly Ile Thr Cys Asp Lys Asn Glu Ser Ala 355 360 365 Ser Gln Gly Gly Ala Ile Phe Gly Lys Asn Cys Gln Ile Ser Asp Asn 370 375 380 Glu Gly Pro Val Val Phe Arg Asp Ser Thr Ala Cys Leu Gly Gly Gly 385 390 395 400 Ala Ile Ala Ala Gln Glu Ile Val Ser Ile Gln Asn Asn Gln Ala Gly 405 410 415 Ile Ser Phe Glu Gly Gly Lys Ala Ser Phe Gly Gly Gly Ile Ala Cys 420 425 430 Gly Ser Phe Ser Ser Ala Gly Gly Ala Ser Val Leu Gly Thr Ile Asp 435 440 445 Ile Ser Lys Asn Leu Gly Ala Ile Ser Phe Ser Arg Thr Leu Cys Thr 450 455 460 Thr Ser Asp Leu Gly Gln Met Glu Tyr Gln Gly Gly Gly Ala Leu Phe 465 470 475 480 Gly Glu Asn Ile Ser Leu Ser Glu Asn Ala Gly Val Leu Thr Phe Lys 485 490 495 Asp Asn Ile Val Lys Thr Phe Ala Ser Asn Gly Lys Ile Leu Gly Gly 500 505 510 Gly Ala Ile Leu Ala Thr Gly Lys Val Glu Ile Thr Asn Asn Ser Glu 515 520 525 Gly Ile Ser Phe Thr Gly Asn Ala Arg Ala Pro Gln Ala Leu Pro Thr 530 535 540 Gln Glu Glu Phe Pro Leu Phe Ser Lys Lys Glu Gly Arg Pro Leu Ser 545 550 555 560 Ser Gly Tyr Ser Gly Gly Gly Ala Ile Leu Gly Arg Glu Val Ala Ile 565 570 575 Leu His Asn Ala Ala Val Val Phe Glu Gln Asn Arg Leu Gln Cys Ser 580 585 590 Glu Glu Glu Ala Thr Leu Leu Gly Cys Cys Gly Gly Gly Ala Val His 595 600 605 Gly Met Asp Ser Thr Ser Ile Val Gly Asn Ser Ser Val Arg Phe Gly 610 615 620 Asn Asn Tyr Ala Met Gly Gln Gly Val Ser Gly Gly Ala Leu Leu Ser 625 630 635 640 Lys Thr Val Gln Leu Ala Gly Asn Gly Ser Val Asp Phe Ser Arg Asn 645 650 655 Ile Ala Ser Leu Gly Gly Gly Ala Leu Gln Ala Ser Glu Gly Asn Cys 660 665 670 Glu Leu Val Asp Asn Gly Tyr Val Leu Phe Arg Asp Asn Arg Gly Arg 675 680 685 Val Tyr Gly Gly Ala Ile Ser Cys Leu Arg Gly Asp Val Val Ile Ser 690 695 700 Gly Asn Lys Gly Arg Val Glu Phe Lys Asp Asn Ile Ala Thr Arg Leu 705 710 715 720 Tyr Val Glu Glu Thr Val Glu Lys Val Glu Glu Val Glu Pro Ala Pro 725 730 735 Glu Gln Lys Asp Asn Asn Glu Leu Ser Phe Leu Gly Arg Ala Glu Gln 740 745 750 Ser Phe Ile Thr Ala Ala Asn Gln Ala Leu Phe Ala Ser Glu Asp Gly 755 760 765 Asp Leu Ser Pro Glu Ser Ser Ile Ser Ser Glu Glu Leu Ala Lys Arg 770 775 780 Arg Glu Cys Ala Gly Gly Ala Ile Phe Ala Lys Arg Val Arg Ile Val 785 790 795 800 Asp Asn Gln Glu Ala Val Val Phe Ser Asn Asn Phe Ser Asp Ile Tyr 805 810 815 Gly Gly Ala Ile Phe Thr Gly Ser Leu Arg Glu Glu Asp Lys Leu Asp 820 825 830 Gly Gln Ile Pro Glu Val Leu Ile Ser Gly Asn Ala Gly Asp Val Val 835 840 845 Phe Ser Gly Asn Ser Ser Lys Arg Asp Glu His Leu Pro His Thr Gly 850 855 860 Gly Gly Ala Ile Cys Thr Gln Asn Leu Thr Ile Ser Gln Asn Thr Gly 865 870 875 880 Asn Val Leu Phe Tyr Asn Asn Val Ala Cys Ser Gly Gly Ala Val Arg 885 890 895 Ile Glu Asp His Gly Asn Val Leu Leu Glu Ala Phe Gly Gly Asp Ile 900 905 910 Val Phe Lys Gly Asn Ser Ser Phe Arg Ala Gln Gly Ser Asp Ala Ile 915 920 925 Tyr Phe Ala Gly Lys Glu Ser His Ile Thr Ala Leu Asn Ala Thr Glu 930 935 940 Gly His Ala Ile Val Phe His Asp Ala Leu Val Phe Glu Asn Leu Glu 945 950 955 960 Glu Arg Lys Ser Ala Glu Val Leu Leu Ile Asn Ser Arg Glu Asn Pro 965 970 975 Gly Tyr Thr Gly Ser Ile Arg Phe Leu Glu Ala Glu Ser Lys Val Pro 980 985 990 Gln Cys Ile His Val Gln Gln Gly Ser Leu Glu Leu Leu Asn Gly Ala 995 1000 1005 Thr Leu Cys Ser Tyr Gly Phe Lys Gln Asp Ala Gly Ala Lys Leu Val 1010 1015 1020 Leu Ala Ala Gly Ala Lys Leu Lys Ile Leu Asp Ser Gly Thr Pro Val 1025 1030 1035 1040 Gln Gln Gly His Ala Ile Ser Lys Pro Glu Ala Glu Ile Glu Ser Ser 1045 1050 1055 Ser Glu Pro Glu Gly Ala His Ser Leu Trp Ile Ala Lys Asn Ala Gln 1060 1065 1070 Thr Thr Val Pro Met Val Asp Ile His Thr Ile Ser Val Asp Leu Ala 1075 1080 1085 Ser Phe Ser Ser Ser Gln Gln Glu Gly Thr Val Glu Ala Pro Gln Val 1090 1095 1100 Ile Val Pro Gly Gly Ser Tyr Val Arg Ser Gly Glu Leu Asn Leu Glu 1105 1110 1115 1120 Leu Val Asn Thr Thr Gly Thr Gly Tyr Glu Asn His Ala Leu Leu Lys 1125 1130 1135 Asn Glu Ala Lys Val Pro Leu Met Ser Phe Val Ala Ser Gly Asp Glu 1140 1145 1150 Ala Ser Ala Glu Ile Ser Asn Leu Ser Val Ser Asp Leu Gln Ile His 1155 1160 1165 Val Val Thr Pro Glu Ile Glu Glu Asp Thr Tyr Gly His Met Gly Asp 1170 1175 1180 Trp Ser Glu Ala Lys Ile Gln Asp Gly Thr Leu Val Ile Ser Trp Asn 1185 1190 1195 1200 Pro Thr Gly Tyr Arg Leu Asp Pro Gln Lys Ala Gly Ala Leu Val Phe 1205 1210 1215 Asn Ala Leu Trp Glu Glu Gly Ala Val Leu Ser Ala Leu Lys Asn Ala 1220 1225 1230 Arg Phe Ala His Asn Leu Thr Ala Gln Arg Met Glu Phe Asp Tyr Ser 1235 1240 1245 Thr Asn Val Trp Gly Phe Ala Phe Gly Gly Phe Arg Thr Leu Ser Ala 1250 1255 1260 Glu Asn Leu Val Ala Ile Asp Gly Tyr Lys Gly Ala Tyr Gly Gly Ala 1265 1270 1275 1280 Ser Ala Gly Val Asp Ile Gln Leu Met Glu Asp Phe Val Leu Gly Val 1285 1290 1295 Ser Gly Ala Ala Phe Leu Gly Lys Met Asp Ser Gln Lys Phe Asp Ala 1300 1305 1310 Glu Val Ser Arg Lys Gly Val Val Gly Ser Val Tyr Thr Gly Phe Leu 1315 1320 1325 Ala Gly Ser Trp Phe Phe Lys Gly Gln Tyr Ser Leu Gly Glu Thr Gln 1330 1335 1340 Asn Asp Met Lys Thr Arg Tyr Gly Val Leu Gly Glu Ser Ser Ala Ser 1345 1350 1355 1360 Trp Thr Ser Arg Gly Val Leu Ala Asp Ala Leu Val Glu Tyr Arg Ser 1365 1370 1375 Leu Val Gly Pro Val Arg Pro Thr Phe Tyr Ala Leu His Phe Asn Pro 1380 1385 1390 Tyr Val Glu Val Ser Tyr Ala Ser Met Lys Phe Pro Gly Phe Thr Glu 1395 1400 1405 Gln Gly Arg Glu Ala Arg Ser Phe Glu Asp Ala Ser Leu Thr Asn Ile 1410 1415 1420 Thr Ile Pro Leu Gly Met Lys Phe Glu Leu Ala Phe Ile Lys Gly Gln 1425 1430 1435 1440 Phe Ser Glu Val Asn Ser Leu Gly Ile Ser Tyr Ala Trp Glu Ala Tyr 1445 1450 1455 Arg Lys Val Glu Gly Gly Ala Val Gln Leu Leu Glu Ala Gly Phe Asp 1460 1465 1470 Trp Glu Gly Ala Pro Met Asp Leu Pro Arg Gln Glu Leu Arg Val Ala 1475 1480 1485 Leu Glu Asn Asn Thr Glu Trp Ser Ser Tyr Phe Ser Thr Val Leu Gly 1490 1495 1500 Leu Thr Ala Phe Cys Gly Gly Phe Thr Ser Thr Asp Ser Lys Leu Gly 1505 1510 1515 1520 Tyr Glu Ala Asn Thr Gly Leu Arg Leu Ile Phe 1525 1530 16 4596 DNA Chlamydia trachomatis 16 atgagttccg agaaagatat aaaaagcacc tgttctaagt tttctttgtc tgtagtagca 60 gctatccttg cctctgttag cgggttagct agttgcgtag atcttcatgc tggaggacag 120 tctgtaaatg agctggtata tgtaggccct caagcggttt tattgttaga ccaaattcga 180 gatctattcg ttgggtctaa agatagtcag gctgaaggac agtataggtt aattgtagga 240 gatccaagtt ctttccaaga gaaagatgcg gatactcttc ccgggaaggt agagcaaagt 300 actttgttct cagtaaccaa tcccgtggtt ttccaaggtg tggaccaaca ggatcaagtc 360 tcttcccaag ggttaatttg tagttttacg agcagcaacc ttgattctcc tcgtgacgga 420 gaatcttttt taggtattgc ttttgttggg gatagtagta aggctggaat cacattaact 480 gacgtgaaag cttctttgtc tggagcggct ttatattcta cagaagatct tatctttgaa 540 aagattaagg gtggattgga atttgcatca tgttcttctc tagaacaggg gggagcttgt 600 gcagctcaaa gtattttgat tcatgattgt caaggattgc aggttaaaca ctgtactaca 660 gccgtgaatg ctgaggggtc tagtgcgaat gatcatcttg gatttggagg aggcgctttc 720 tttgttacgg gttctctttc tggagagaaa agtctctata tgcctgcagg agatatggta 780 gttgcgaatt gtgatggggc tatatctttt gaaggaaaca gcgcgaactt tgctaatgga 840 ggagcgattg ctgcctctgg gaaagtgctt tttgtcgcta atgataaaaa gacttctttt 900 atagagaacc gagctttgtc tggaggagcg attgcagcct cttctgatat tgcctttcaa 960 aactgcgcag aactagtttt caaaggcaat tgtgcaattg gaacagagga taaaggttct 1020 ttaggtggag gggctatatc ttctctaggc accgttcttt tgcaagggaa tcacgggata 1080 acttgtgata agaatgagtc tgcttcgcaa ggaggcgcca tttttggcaa aaattgtcag 1140 atttctgaca acgaggggcc agtggttttc agagatagta cagcttgctt aggaggaggc 1200 gctattgcag ctcaagaaat tgtttctatt cagaacaatc aggctgggat ttccttcgag 1260 ggaggtaagg ctagtttcgg aggaggtatt gcgtgtggat ctttttcttc cgcaggtggt 1320 gcttctgttt tagggaccat tgatatttcg aagaatttag gcgcgatttc gttctctcgt 1380 actttatgta cgacctcaga tttaggacaa atggagtacc agggaggagg agctctattt 1440 ggtgaaaata tttctctttc tgagaatgct ggtgtgctca cctttaaaga caacattgtg 1500 aagacttttg cttcgaatgg gaaaattctg ggaggaggag cgattttagc tactggtaag 1560 gtggaaatta ctaataattc cgaaggaatt tcttttacag gaaatgcgag agctccacaa 1620 gctcttccaa ctcaagagga gtttccttta ttcagcaaaa aagaagggcg accactctct 1680 tcaggatatt ctgggggagg agcgatttta ggaagagaag tagctattct ccacaacgct 1740 gcagtagtat ttgagcaaaa tcgtttgcag tgcagcgaag aagaagcgac attattaggt 1800 tgttgtggag gaggcgctgt tcatgggatg gatagcactt cgattgttgg caactcttca 1860 gtaagatttg gtaataatta cgcaatggga caaggagtct caggaggagc tcttttatct 1920 aaaacagtgc agttagctgg gaatggaagc gtcgattttt ctcgaaatat tgctagtttg 1980 ggaggaggag ctcttcaagc ttctgaagga aattgtgagc tagttgataa cggctatgtg 2040 ctattcagag ataatcgagg gagggtttat gggggtgcta tttcttgctt acgtggagat 2100 gtagtcattt ctggaaacaa gggtagagtt gaatttaaag acaacatagc aacacgtctt 2160 tatgtggaag aaactgtaga aaaggttgaa gaggtagagc cagctcctga gcaaaaagac 2220 aataatgagc tttctttctt agggagagca gaacagagtt ttattactgc agctaatcaa 2280 gctcttttcg catctgaaga tggggattta tcacctgagt catccatttc ttctgaagaa 2340 cttgcgaaaa gaagagagtg tgctggagga gctatttttg caaaacgggt tcgtattgta 2400 gataaccaag aggccgttgt attctcgaat aacttctctg atatttatgg cggcgccatt 2460 tttacaggtt ctcttcgaga agaggataag ttagatgggc aaatccctga agtcttgatc 2520 tcaggcaatg caggggatgt tgttttttcc ggaaattcct cgaagcgtga tgagcatctt 2580 cctcatacag gtgggggagc catttgtact caaaatttga cgatttctca gaatacaggg 2640 aatgttctgt tttataacaa cgtggcctgt tcgggaggag ctgttcgtat agaggatcat 2700 ggtaatgttc ttttagaagc ttttggagga gatattgttt ttaaaggaaa ttcttctttc 2760 agagcacaag gatccgatgc tatctatttt gcaggtaaag aatcgcatat tacagccctg 2820 aatgctacgg aaggacatgc tattgttttc cacgacgcat tagtttttga aaatctagaa 2880 gaaaggaaat ctgctgaagt attgttaatc aatagtcgag aaaatccagg ttacactgga 2940 tctattcgat ttttagaagc agaaagtaaa gttcctcaat gtattcatgt acaacaagga 3000 agccttgagt tgctaaatgg agccacatta tgtagttatg gttttaaaca agatgctgga 3060 gctaagttgg tattggctgc tggagctaaa ctgaagattt tagattcagg aactcctgta 3120 caacaagggc atgctatcag taaacctgaa gcagaaatcg agtcatcttc tgaaccagag 3180 ggtgcacatt ctctttggat tgcgaagaat gctcaaacaa cagttcctat ggttgatatc 3240 catactattt ctgtagattt agcctccttc tcttctagtc aacaggaggg gacagtagaa 3300 gctcctcagg ttattgttcc tggaggaagt tatgttcgat ctggagagct taatttggag 3360 ttagttaaca caacaggtac tggttatgaa aatcatgctt tattgaagaa tgaggctaaa 3420 gttccattga tgtctttcgt tgcttctggt gatgaagctt cagccgaaat cagtaacttg 3480 tcggtttctg atttacagat tcatgtagta actccagaga ttgaagaaga cacatacggc 3540 catatgggag attggtctga ggctaaaatt caagatggaa ctcttgtcat tagttggaat 3600 cctactggat atcgattaga tcctcaaaaa gcaggggctt tagtatttaa tgcattatgg 3660 gaagaagggg ctgtcttgtc tgctctgaaa aatgcacgct ttgctcataa tctcactgct 3720 cagcgtatgg aattcgatta ttctacaaat gtgtggggat tcgcctttgg tggtttccga 3780 actctatctg cagagaatct ggttgctatt gatggataca aaggagctta tggtggtgct 3840 tctgctggag tcgatattca attgatggaa gattttgttc taggagttag tggagctgct 3900 ttcctaggta aaatggatag tcagaagttt gatgcggagg tttctcggaa gggagttgtt 3960 ggttctgtat atacaggatt tttagctgga tcctggttct tcaaaggaca atatagcctt 4020 ggagaaacac agaacgatat gaaaacgcgt tatggagtac taggagagtc gagtgcttct 4080 tggacatctc gaggagtact ggcagatgct ttagttgaat accgaagttt agttggtcct 4140 gtgagaccta ctttttatgc tttgcatttc aatccttatg tcgaagtatc ttatgcttct 4200 atgaaattcc ctggctttac agaacaagga agagaagcgc gttcttttga agacgcttcc 4260 cttaccaata tcaccattcc tttagggatg aagtttgaat tggcgttcat aaaaggacag 4320 ttttcagagg tgaactcttt gggaataagt tatgcatggg aagcttatcg aaaagtagaa 4380 ggaggcgcgg tgcagctttt agaagctggg tttgattggg agggagctcc aatggatctt 4440 cctagacagg agctgcgtgt cgctctggaa aataatacgg aatggagttc ttacttcagc 4500 acagtcttag gattaacagc tttttgtgga ggatttactt ctacagatag taaactagga 4560 tatgaggcga atactggatt gcgattgatc ttttaa 4596 17 340 PRT Chlamydia trachomatis 17 Met Ser Ser Lys Leu Val Asn Tyr Leu Arg Leu Thr Phe Leu Ser Phe 1 5 10 15 Leu Gly Ile Ala Ser Thr Ser Leu Asp Ala Met Pro Ala Gly Asn Pro 20 25 30 Ala Phe Pro Val Ile Pro Gly Ile Asn Ile Glu Gln Lys Asn Ala Cys 35 40 45 Ser Phe Asp Leu Cys Asn Ser Tyr Asp Val Leu Ser Ala Leu Ser Gly 50 55 60 Asn Leu Lys Leu Cys Phe Cys Gly Asp Tyr Ile Phe Ser Glu Glu Ala 65 70 75 80 Gln Val Lys Asp Val Pro Val Val Thr Ser Val Thr Thr Ala Gly Val 85 90 95 Gly Pro Ser Pro Asp Ile Thr Ser Thr Thr Lys Thr Arg Asn Phe Asp 100 105 110 Leu Val Asn Cys Asn Leu Asn Thr Asn Cys Val Ala Val Ala Phe Ser 115 120 125 Leu Pro Asp Arg Ser Leu Ser Ala Ile Pro Leu Phe Asp Val Ser Phe 130 135 140 Glu Val Lys Val Gly Gly Leu Lys Gln Tyr Tyr Arg Leu Pro Met Asn 145 150 155 160 Ala Tyr Arg Asp Phe Thr Ser Glu Pro Leu Asn Ser Glu Ser Glu Val 165 170 175 Thr Asp Gly Met Ile Glu Val Gln Ser Asn Tyr Gly Phe Val Trp Asp 180 185 190 Val Ser Leu Lys Lys Val Ile Trp Lys Asp Gly Val Ser Phe Val Gly 195 200 205 Val Gly Ala Asp Tyr Arg His Ala Ser Cys Pro Ile Asp Tyr Ile Ile 210 215 220 Ala Asn Ser Gln Ala Asn Pro Glu Val Phe Ile Ala Asp Ser Asp Gly 225 230 235 240 Lys Leu Asn Phe Lys Glu Trp Ser Val Cys Val Gly Leu Thr Thr Tyr 245 250 255 Val Asn Asp Tyr Val Leu Pro Tyr Leu Ala Phe Ser Ile Gly Ser Val 260 265 270 Ser Arg Gln Ala Pro Asp Asp Ser Phe Lys Lys Leu Glu Asp Arg Phe 275 280 285 Thr Asn Leu Lys Phe Lys Val Arg Lys Ile Thr Ser Ser His Arg Gly 290 295 300 Asn Ile Cys Ile Gly Ala Thr Asn Tyr Val Ala Asp Asn Phe Phe Tyr 305 310 315 320 Asn Val Glu Gly Arg Trp Gly Ser Gln Arg Ala Val Asn Val Ser Gly 325 330 335 Gly Phe Gln Phe 340 18 1023 DNA Chlamydia trachomatis 18 atgagtagca agctagtgaa ctatctccgt ttgactttcc tatctttttt agggatcgca 60 tctacttcat tagacgctat gcctgcgggg aatccggcgt ttccagtcat cccggggatt 120 aatattgaac agaaaaatgc ctgttctttc gatttatgta attcttatga tgtactatcc 180 gcactgtccg gtaacctgaa gctctgcttc tgcggagatt atatcttttc agaagaagct 240 caggtaaaag atgtccctgt cgttacctct gtgacaacag ctggggttgg tccttctcct 300 gatattactt cgacaaccaa aacgcgaaat ttcgatctcg tgaactgtaa tctcaataca 360 aactgtgtag ctgtagcttt ttcccttcct gatcgttcgc tgagcgcgat tcctctgttt 420 gatgtgagtt tcgaagtgaa agtaggagga ctgaaacaat actaccgcct tcccatgaat 480 gcctatcgag acttcacctc ggaacctctc aattctgaat cagaagttac ggacgggatg 540 attgaagtac agtccaatta cggatttgtt tgggatgtta gcttgaaaaa agtcatatgg 600 aaagatggcg tttcctttgt aggcgtcggt gcagactatc gccatgcttc ttgccctatt 660 gactacatca ttgcaaacag tcaagctaat ccagaagtat tcatcgctga ctcggatggg 720 aaactgaact tcaaggagtg gagtgtctgc gtaggtctta ctacctatgt gaatgactac 780 gttcttcctt acttagcgtt ttctataggg agtgtttctc gccaagctcc ggacgacagc 840 ttcaaaaaat tagaagatcg cttcactaac ctcaaattta aagttcgtaa aattaccagc 900 tctcatcgtg gaaacatctg catcggagcg acaaactatg tcgccgataa cttcttctac 960 aacgtagaag gaagatgggg aagccagcgc gctgtgaacg tctccggagg attccaattc 1020 taa 1023 19 350 PRT Chlamydia trachomatis 19 Met Ser Leu Phe Ser Lys Phe Lys Ala Gln Trp Met Phe Leu His Ser 1 5 10 15 Arg Glu Leu Cys Ser Ser Thr Ser Asp Ile Gly Asn Thr Cys Ser Asp 20 25 30 Pro Val Phe Gln Val Leu Cys Asn Pro Val Arg Ser Glu Ile Ser Tyr 35 40 45 Lys Val Gly Asp Ser Leu Gly Val Phe Pro Thr Asn Pro Ser Ile Leu 50 55 60 Val Asp Ser Val Leu Asp Ala Leu Gln Tyr Gly Pro Arg Ser Pro Val 65 70 75 80 Val Ser Arg His Ala Asp Ser Val Leu Pro Leu His Glu Phe Leu Thr 85 90 95 Ser Tyr Val Asp Leu Asp Lys Ile Pro Lys Ser Leu Arg Pro Phe Phe 100 105 110 Pro Gly Asp Leu Asp Asp Thr Trp Ser Leu Ala Glu Ala Ile Leu Val 115 120 125 Tyr Gln Pro Arg Ile Pro Phe Glu Glu Phe Ile Arg Ser Ala Met Pro 130 135 140 Leu Leu Pro Arg Phe Tyr Ser Ile Ala Ser Ser Pro Thr Cys Ser His 145 150 155 160 Gly Lys Leu Glu Leu Leu Val Arg Cys Val Ser Phe Gln Gly Lys Thr 165 170 175 Gln Leu Arg Tyr Gly Leu Cys Ser Ala Phe Leu Cys Lys Asp Leu Gln 180 185 190 Glu Gly Glu Ser Phe Arg Gly Phe Ile Gln Pro Thr Arg His Phe Thr 195 200 205 Leu Glu Gln Lys Asn Phe Gly Lys Pro Leu Ile Met Ile Gly Ala Gly 210 215 220 Thr Gly Ile Ala Pro Tyr Lys Gly Phe Leu Gln His Arg Ile Tyr His 225 230 235 240 Gln Asp Val Gly Ser Asn Ile Leu Phe Phe Gly Glu Arg Phe Glu Lys 245 250 255 Ser Asn Phe Tyr Tyr Arg Asp Phe Leu Gln Glu Leu Ile Val Ser Gly 260 265 270 Lys Leu Gln Leu Phe Thr Ala Phe Ser Arg Asp Ser Glu Ser Lys Leu 275 280 285 Tyr Val Gln Asn Val Ile Glu Gln Gln Lys Glu Leu Ile Gln Glu Val 290 295 300 Tyr Glu Gln Glu Ala Phe Phe Phe Val Cys Gly Lys Lys Ile Leu Gly 305 310 315 320 Thr Glu Val Lys Arg Ala Leu Glu Gln Ile Leu Gly Pro Lys Ala Val 325 330 335 Arg Glu Leu Ile Ala Gln Lys Arg Leu Val Ser Asp Val Tyr 340 345 350 20 1053 DNA Chlamydia trachomatis 20 atgtctttat tttctaaatt caaagctcag tggatgtttt tacattcacg tgagctttgt 60 tcttccacat cggatattgg gaatacttgt tcggatcctg tttttcaggt tttatgtaat 120 ccggttcgtt ctgagatttc ctataaagtt ggggattctt tgggggtatt cccaacaaat 180 ccttccatat tagtcgattc agttctagat gctttacagt atggccccag gtctcctgtc 240 gtatctcggc atgcagattc tgttctccct cttcacgaat ttcttactag ttacgtagac 300 ttagataaaa ttccaaaatc gttaagacct tttttcccag gggatttaga cgatacctgg 360 tctttagctg aagctatttt ggtttaccag ccgcgtattc cttttgaaga gtttattcgg 420 agtgcgatgc ctttattgcc tcgattttat tctatagctt cttctccaac atgttctcat 480 gggaagctag agttgctcgt gcgctgtgtt agtttccaag gtaaaacgca gctgcgctat 540 ggattatgtt cggctttttt atgtaaggac ttacaagagg gagagtcttt tcgtgggttt 600 atacaaccga cgcggcattt tactttggag cagaaaaatt ttgggaaacc tttaattatg 660 atcggagcag ggacaggtat cgctccgtac aaagggttct tacaacatcg aatataccat 720 caggacgtag gctccaatat tctattcttt ggagagcgtt ttgagaaaag taacttctat 780 taccgggatt ttctccagga gctgatcgtt tcaggaaaac tccagttatt cacagccttt 840 tccagagatt ccgagtctaa attgtatgtt cagaatgtta tagagcaaca aaaagaactt 900 atacaagaag tctacgaaca agaagctttc ttttttgttt gtgggaaaaa aatccttggt 960 acggaagtta aacgtgcttt agagcagata ttaggtccta aggcggtacg agagctgatt 1020 gcacagaaga gactagtttc agacgtatac taa 1053 21 1770 PRT Chlamydia trachomatis 21 Met Lys Phe Met Ser Ala Thr Ala Val Phe Ala Ala Ala Leu Ser Ser 1 5 10 15 Val Thr Glu Ala Ser Ser Ile Gln Asp Gln Ile Lys Asn Thr Asp Cys 20 25 30 Asn Val Ser Lys Leu Gly Tyr Ser Thr Ser Gln Ala Phe Thr Asp Met 35 40 45 Met Leu Ala Asp Asn Thr Glu Tyr Arg Ala Ala Asp Ser Val Ser Phe 50 55 60 Tyr Asp Phe Ser Thr Ser Ser Arg Leu Pro Arg Lys His Leu Ser Ser 65 70 75 80 Ser Ser Glu Ala Ser Pro Thr Thr Glu Gly Val Ser Ser Ser Ser Ser 85 90 95 Gly Glu Thr Asp Glu Lys Thr Glu Glu Glu Leu Asp Asn Gly Gly Ile 100 105 110 Ile Tyr Ala Arg Glu Lys Leu Thr Ile Ser Glu Ser Gln Asp Ser Leu 115 120 125 Ser Asn Gln Ser Ile Glu Leu His Asp Asn Ser Ile Phe Phe Gly Glu 130 135 140 Gly Glu Val Ile Phe Asp His Arg Val Ala Leu Lys Asn Gly Gly Ala 145 150 155 160 Ile Tyr Gly Glu Lys Glu Val Val Phe Glu Asn Ile Lys Ser Leu Leu 165 170 175 Val Glu Val Asn Ile Ala Val Glu Lys Gly Gly Ser Val Tyr Ala Lys 180 185 190 Glu Arg Val Ser Leu Glu Asn Val Thr Glu Ala Thr Phe Ser Ser Asn 195 200 205 Gly Gly Glu Gln Gly Gly Gly Gly Ile Tyr Ser Glu Gln Asp Met Leu 210 215 220 Ile Ser Asp Cys Asn Asn Val His Phe Gln Gly Asn Ala Ala Gly Ala 225 230 235 240 Thr Ala Val Lys Gln Cys Leu Asp Glu Glu Met Ile Val Leu Leu Ala 245 250 255 Glu Cys Val Asp Ser Leu Ser Glu Asp Thr Leu Asp Ser Thr Pro Glu 260 265 270 Thr Glu Gln Thr Glu Ser Asn Gly Asn Gln Asp Gly Ser Ser Glu Thr 275 280 285 Glu Asp Thr Gln Val Ser Glu Ser Pro Glu Ser Thr Pro Ser Pro Asp 290 295 300 Asp Val Leu Gly Lys Gly Gly Gly Ile Tyr Thr Glu Lys Ser Leu Thr 305 310 315 320 Ile Thr Gly Ile Thr Gly Thr Ile Asp Phe Val Ser Asn Ile Ala Thr 325 330 335 Asp Ser Gly Ala Gly Val Phe Thr Lys Glu Asn Leu Ser Cys Thr Asn 340 345 350 Thr Asn Ser Leu Gln Phe Leu Lys Asn Ser Ala Gly Gln His Gly Gly 355 360 365 Gly Ala Tyr Val Thr Gln Thr Met Ser Val Thr Asn Thr Thr Ser Glu 370 375 380 Ser Ile Thr Thr Pro Pro Leu Ile Gly Glu Val Ile Phe Ser Glu Asn 385 390 395 400 Thr Ala Lys Gly His Gly Gly Gly Ile Cys Thr Asn Lys Leu Ser Leu 405 410 415 Ser Asn Leu Lys Thr Val Thr Leu Thr Lys Asn Ser Ala Lys Glu Ser 420 425 430 Gly Gly Ala Ile Phe Thr Asp Leu Ala Ser Ile Pro Ile Thr Asp Thr 435 440 445 Pro Glu Ser Ser Thr Pro Ser Ser Ser Ser Pro Ala Ser Thr Pro Glu 450 455 460 Val Val Ala Ser Ala Lys Ile Asn Arg Phe Phe Ala Ser Thr Ala Lys 465 470 475 480 Pro Ala Ala Pro Ser Leu Thr Glu Ala Glu Ser Asp Gln Thr Asp Gln 485 490 495 Thr Glu Thr Ser Asp Thr Asn Ser Asp Ile Asp Val Ser Ile Glu Asn 500 505 510 Ile Leu Asn Val Ala Ile Asn Gln Asn Thr Ser Ala Lys Lys Gly Gly 515 520 525 Ala Ile Tyr Gly Lys Lys Ala Lys Leu Ser Arg Ile Asn Asn Leu Glu 530 535 540 Leu Ser Gly Asn Ser Ser Gln Asp Val Gly Gly Gly Leu Cys Leu Thr 545 550 555 560 Glu Ser Val Glu Phe Asp Ala Ile Gly Ser Leu Leu Ser His Tyr Asn 565 570 575 Ser Ala Ala Lys Glu Gly Gly Ala Ile His Ser Lys Thr Val Thr Leu 580 585 590 Ser Asn Leu Lys Ser Thr Phe Thr Phe Ala Asp Asn Thr Val Lys Ala 595 600 605 Ile Val Glu Ser Thr Pro Glu Ala Pro Glu Glu Ile Pro Pro Val Glu 610 615 620 Gly Glu Glu Ser Thr Ala Thr Glu Asp Pro Asn Ser Asn Thr Glu Gly 625 630 635 640 Ser Ser Ala Asn Thr Asn Leu Glu Gly Ser Gln Gly Asp Thr Ala Asp 645 650 655 Thr Gly Thr Gly Asp Val Asn Asn Glu Ser Gln Asp Thr Ser Asp Thr 660 665 670 Gly Asn Ala Glu Ser Glu Glu Gln Leu Gln Asp Ser Thr Gln Ser Asn 675 680 685 Glu Glu Asn Thr Leu Pro Asn Ser Asn Ile Asp Gln Ser Asn Glu Asn 690 695 700 Thr Asp Glu Ser Ser Asp Ser His Thr Glu Glu Ile Thr Asp Glu Ser 705 710 715 720 Val Ser Ser Ser Ser Glu Ser Gly Ser Ser Thr Pro Gln Asp Gly Gly 725 730 735 Ala Ala Ser Ser Gly Ala Pro Ser Gly Asp Gln Ser Ile Ser Ala Asn 740 745 750 Ala Cys Leu Ala Lys Ser Tyr Ala Ala Ser Thr Asp Ser Ser Pro Val 755 760 765 Ser Asn Ser Ser Gly Ser Glu Glu Pro Val Thr Ser Ser Ser Asp Ser 770 775 780 Asp Val Thr Ala Ser Ser Asp Asn Pro Asp Ser Ser Ser Ser Gly Asp 785 790 795 800 Ser Ala Gly Asp Ser Glu Glu Pro Thr Glu Pro Glu Ala Gly Ser Thr 805 810 815 Thr Glu Thr Leu Thr Leu Ile Gly Gly Gly Ala Ile Tyr Gly Glu Thr 820 825 830 Val Lys Ile Glu Asn Phe Ser Gly Gln Gly Ile Phe Ser Gly Asn Lys 835 840 845 Ala Ile Asp Asn Thr Thr Glu Gly Ser Ser Ser Lys Ser Asp Val Leu 850 855 860 Gly Gly Ala Val Tyr Ala Lys Thr Leu Phe Asn Leu Asp Ser Gly Ser 865 870 875 880 Ser Arg Arg Thr Val Thr Phe Ser Gly Asn Thr Val Ser Ser Gln Ser 885 890 895 Thr Thr Gly Gln Val Ala Gly Gly Ala Ile Tyr Ser Pro Thr Val Thr 900 905 910 Ile Ala Thr Pro Val Val Phe Ser Lys Asn Ser Ala Thr Asn Asn Ala 915 920 925 Asn Asn Thr Thr Asp Thr Gln Arg Lys Asp Thr Phe Gly Gly Ala Ile 930 935 940 Gly Ala Thr Ser Ala Val Ser Leu Ser Gly Gly Ala His Phe Leu Glu 945 950 955 960 Asn Val Ala Asp Leu Gly Ser Ala Ile Gly Leu Val Pro Gly Thr Gln 965 970 975 Asn Thr Glu Thr Val Lys Leu Glu Ser Gly Ser Tyr Tyr Phe Glu Lys 980 985 990 Asn Lys Ala Leu Lys Arg Ala Thr Ile Tyr Ala Pro Val Val Ser Ile 995 1000 1005 Lys Ala Tyr Thr Ala Thr Phe Asn Gln Asn Arg Ser Leu Glu Glu Gly 1010 1015 1020 Ser Ala Ile Tyr Phe Thr Lys Glu Ala Ser Ile Glu Ser Leu Gly Ser 1025 1030 1035 1040 Val Leu Phe Thr Gly Asn Leu Val Thr Leu Thr Leu Ser Thr Thr Thr 1045 1050 1055 Glu Gly Thr Pro Ala Thr Thr Ser Gly Asp Val Thr Lys Tyr Gly Ala 1060 1065 1070 Ala Ile Phe Gly Gln Ile Ala Ser Ser Asn Gly Ser Gln Thr Asp Asn 1075 1080 1085 Leu Pro Leu Lys Leu Ile Ala Ser Gly Gly Asn Ile Cys Phe Arg Asn 1090 1095 1100 Asn Glu Tyr Arg Pro Thr Ser Ser Asp Thr Gly Thr Ser Thr Phe Cys 1105 1110 1115 1120 Ser Ile Ala Gly Asp Val Lys Leu Thr Met Gln Ala Ala Lys Gly Lys 1125 1130 1135 Thr Ile Ser Phe Phe Asp Ala Ile Arg Thr Ser Thr Lys Lys Thr Gly 1140 1145 1150 Thr Gln Ala Thr Ala Tyr Asp Thr Leu Asp Ile Asn Lys Ser Glu Asp 1155 1160 1165 Ser Glu Thr Val Asn Ser Ala Phe Thr Gly Thr Ile Leu Phe Ser Ser 1170 1175 1180 Glu Leu His Glu Asn Lys Ser Tyr Ile Pro Gln Asn Val Val Leu His 1185 1190 1195 1200 Ser Gly Ser Leu Val Leu Lys Pro Asn Thr Glu Leu His Val Ile Ser 1205 1210 1215 Phe Glu Gln Lys Glu Gly Ser Ser Leu Val Met Thr Pro Gly Ser Val 1220 1225 1230 Leu Ser Asn Gln Thr Val Ala Asp Gly Ala Leu Val Ile Asn Asn Met 1235 1240 1245 Thr Ile Asp Leu Ser Ser Val Glu Lys Asn Gly Ile Ala Glu Gly Asn 1250 1255 1260 Ile Phe Thr Pro Pro Glu Leu Arg Ile Ile Asp Thr Thr Thr Gly Gly 1265 1270 1275 1280 Ser Gly Gly Thr Pro Ser Thr Asp Ser Glu Ser Asn Gln Asn Ser Asp 1285 1290 1295 Asp Thr Glu Glu Gln Asn Asn Asn Asp Ala Ser Asn Gln Gly Glu Ser 1300 1305 1310 Ala Asn Gly Ser Ser Ser Pro Ala Val Ala Ala Ala His Thr Ser Arg 1315 1320 1325 Thr Arg Asn Phe Ala Ala Ala Ala Thr Ala Thr Pro Thr Thr Thr Pro 1330 1335 1340 Thr Ala Thr Thr Thr Thr Ser Asn Gln Val Ile Leu Gly Gly Glu Ile 1345 1350 1355 1360 Lys Leu Ile Asp Pro Asn Gly Thr Phe Phe Gln Asn Pro Ala Leu Arg 1365 1370 1375 Ser Asp Gln Gln Ile Ser Leu Leu Val Leu Pro Thr Asp Ser Ser Lys 1380 1385 1390 Met Gln Ala Gln Lys Ile Val Leu Thr Gly Asp Ile Ala Pro Gln Lys 1395 1400 1405 Gly Tyr Thr Gly Thr Leu Thr Leu Asp Pro Asp Gln Leu Gln Asn Gly 1410 1415 1420 Thr Ile Ser Val Leu Trp Lys Phe Asp Ser Tyr Arg Gln Trp Ala Tyr 1425 1430 1435 1440 Val Pro Arg Asp Asn His Phe Tyr Ala Asn Ser Ile Leu Gly Ser Gln 1445 1450 1455 Met Leu Met Val Thr Val Lys Gln Gly Leu Leu Asn Asp Lys Met Asn 1460 1465 1470 Leu Ala Arg Phe Glu Glu Val Ser Tyr Asn Asn Leu Trp Ile Ser Gly 1475 1480 1485 Leu Gly Thr Met Leu Ser Gln Val Gly Thr Pro Thr Ser Glu Glu Phe 1490 1495 1500 Thr Tyr Tyr Ser Arg Gly Ala Ser Val Ala Leu Asp Ala Lys Pro Ala 1505 1510 1515 1520 His Asp Val Ile Val Gly Ala Ala Phe Ser Lys Met Ile Gly Lys Thr 1525 1530 1535 Lys Ser Leu Lys Arg Glu Asn Asn Tyr Thr His Lys Gly Ser Glu Tyr 1540 1545 1550 Ser Tyr Gln Ala Ser Val Tyr Gly Gly Lys Pro Phe His Phe Val Ile 1555 1560 1565 Asn Lys Lys Thr Glu Lys Ser Leu Pro Leu Leu Leu Gln Gly Val Ile 1570 1575 1580 Ser Tyr Gly Tyr Ile Lys His Asp Thr Val Thr His Tyr Pro Thr Ile 1585 1590 1595 1600 Arg Glu Arg Asn Lys Gly Glu Trp Glu Asp Leu Gly Trp Leu Thr Ala 1605 1610 1615 Leu Arg Val Ser Ser Val Leu Arg Thr Pro Ala Gln Gly Asp Thr Lys 1620 1625 1630 Arg Ile Thr Val Tyr Gly Glu Leu Glu Tyr Ser Ser Ile Arg Gln Lys 1635 1640 1645 Gln Phe Thr Glu Thr Glu Tyr Asp Pro Arg Tyr Phe Asp Asn Cys Thr 1650 1655 1660 Tyr Arg Asn Leu Ala Ile Pro Met Gly Leu Ala Phe Glu Gly Glu Leu 1665 1670 1675 1680 Ser Gly Asn Asp Ile Leu Met Tyr Asn Arg Phe Ser Val Ala Tyr Met 1685 1690 1695 Leu Ser Ile Tyr Arg Asn Ser Pro Thr Cys Lys Tyr Gln Val Leu Ser 1700 1705 1710 Ser Gly Glu Gly Gly Glu Ile Ile Cys Gly Val Pro Thr Arg Asn Ser 1715 1720 1725 Ala Arg Gly Glu Tyr Ser Thr Gln Leu Tyr Leu Gly Pro Leu Trp Thr 1730 1735 1740 Leu Tyr Gly Ser Tyr Thr Ile Glu Ala Asp Ala His Thr Leu Ala His 1745 1750 1755 1760 Met Met Asn Cys Gly Ala Arg Met Thr Phe 1765 1770 22 5313 DNA Chlamydia trachomatis 22 atgaaattta tgtcagctac tgctgtattt gctgcagcac tctcctccgt tactgaggcg 60 agctcgatcc aagatcaaat aaagaatacc gactgcaatg ttagcaaatt aggatattca 120 acttctcaag catttactga tatgatgcta gcagacaaca cagagtatcg agctgctgat 180 agtgtttcat tctatgactt ttcgacatct tccagattac ctagaaaaca tcttagtagt 240 agtagtgaag cttctccaac gacagaagga gtgtcttcat cttcatctgg agaaactgat 300 gagaaaacag aagaagaact agacaatggc ggaatcattt atgctagaga gaaactaact 360 atctcagaat ctcaggactc tctctctaat caaagcatag aactccatga caatagtatt 420 ttcttcggag aaggtgaagt tatctttgat cacagagttg ccctcaaaaa cggaggagct 480 atttatggag agaaagaggt agtctttgaa aacataaaat ctctactagt agaagtaaat 540 atcgcggtcg agaaaggggg tagcgtctat gcaaaagaac gagtatcttt agaaaatgtt 600 accgaagcaa ccttctcctc caatggtggg gaacaaggtg gtggtggaat ctattcagaa 660 caggatatgt taatcagtga ttgcaacaat gtacatttcc aagggaatgc tgcaggagca 720 acagcagtaa aacaatgtct ggatgaagaa atgatcgtat tgctcgcaga atgcgttgat 780 agcttatccg aagatacact ggatagcact ccagaaacgg aacagactga gtcaaatgga 840 aatcaagacg gttcgtctga aacagaagat acacaagtat cagaatcacc agaatcaact 900 cctagccccg acgatgtttt aggtaaaggt ggtggtatct atacagaaaa atctttgacc 960 atcactggaa ttacagggac tatagatttt gtcagtaaca tagctaccga ttctggagca 1020 ggtgtattca ctaaagaaaa cttgtcttgc accaacacga atagcctaca gtttttgaaa 1080 aactcggcag gtcaacatgg aggaggagcc tacgttactc aaaccatgtc tgttactaat 1140 acaactagtg aaagtataac tactccccct ctcataggag aagtgatttt ctctgaaaat 1200 acagctaaag ggcacggtgg tggtatctgc actaacaaac tttctttatc taatttaaaa 1260 acggtgactc tcactaaaaa ctctgcaaag gagtctggag gagctatttt tacagatctg 1320 gcgtctatac caataacaga taccccagaa tcttctaccc cctcttcctc ctcgcctgca 1380 agcactcctg aagtagttgc ttctgctaaa ataaatcgat tctttgcctc tacggcaaaa 1440 ccggcagccc cttctctaac agaggctgag tctgatcaaa cggatcaaac agaaacttct 1500 gatactaata gcgatataga cgtgtcgatt gagaacattt tgaatgtcgc tatcaatcaa 1560 aacacttctg cgaaaaaagg aggggctatt tacgggaaaa aagctaaact ttcccgtatt 1620 aacaatcttg aactttcagg gaattcatcc caggatgtag gaggaggtct ctgtttaact 1680 gaaagcgtag aatttgatgc aattggatcg ctcttatccc actataactc tgctgctaaa 1740 gaaggtgggg ctattcattc taaaacggtt actctatcta acctcaagtc taccttcact 1800 tttgcagata acactgttaa agcaatagta gaaagcactc ctgaagctcc agaagagatt 1860 cctccagtag aaggagaaga gtctacagca acagaagatc caaattctaa tacagaagga 1920 agttcggcta acactaacct tgaaggatct caaggggata ctgctgatac agggactggt 1980 gatgttaaca atgagtctca agacacatca gatactggaa acgctgaatc tgaagaacaa 2040 ctacaagatt ctacacaatc taatgaagaa aatacccttc ccaatagtaa tattgatcaa 2100 tctaacgaaa acacagacga atcatctgat agccacactg aggaaataac tgacgagagt 2160 gtctcatcgt cctctgaaag tggatcatct actcctcaag atggaggagc agcttcttca 2220 ggggctccct caggagatca atctatctct gcaaacgctt gtttagctaa aagctatgct 2280 gcgagtactg atagctcccc cgtatctaat tcttcaggtt cagaagagcc tgtcacttct 2340 tcttcagatt cagacgttac tgcatcttct gataatccag actcttcctc atctggagat 2400 agcgctggag actctgaaga accgactgag ccagaagctg gttctacaac agaaactctt 2460 actttaatag gaggaggtgc tatctatgga gaaactgtta agattgagaa cttctctggc 2520 caaggaatat tttctggaaa caaagctatc gataacacca cagaaggctc ctcttccaaa 2580 tctgacgtcc tcggaggtgc ggtctatgct aaaacattgt ttaatctcga tagcgggagc 2640 tctagacgaa ctgtcacctt ctccgggaat actgtctctt ctcaatctac aacaggtcag 2700 gttgctggag gagctatcta ctctcctact gtaaccattg ctactcctgt agtattttct 2760 aaaaactctg caacaaacaa tgctaataac actacagata ctcagagaaa agacaccttt 2820 ggaggagcta tcggagctac ttctgctgtt tctctatcag gaggggctca tttcttagaa 2880 aacgttgctg acctcggatc tgctattggg ttggtgccag gcacacaaaa tacagaaaca 2940 gtgaaattag agtctggctc ctactacttt gaaaaaaata aagctttaaa acgagctact 3000 atttacgcac ctgtcgtttc cattaaagcc tatactgcga catttaacca aaacagatct 3060 ctagaagaag gaagcgcgat ttactttaca aaagaagcat ctattgagtc tttaggctct 3120 gttctcttca caggaaactt agtaacccta acgctaagca caactacaga aggcacacca 3180 gccacaacct caggagatgt aacaaaatat ggtgctgcta tctttggaca aatagcaagc 3240 tcaaacggat ctcagacgga taaccttccc ctgaaactca ttgcttcagg aggaaatatt 3300 tgtttccgaa acaatgaata ccgtcctact tcttctgata ccggaacctc tactttctgt 3360 agtattgcgg gagatgttaa attaaccatg caagctgcaa aagggaaaac gatcagtttc 3420 tttgatgcaa tccggacctc tactaagaaa acaggtacac aggcaactgc ctacgatact 3480 ctcgatatta ataaatctga ggattcagaa actgtaaact ctgcgtttac aggaacgatt 3540 ctgttctcct ctgaattaca tgaaaataaa tcctatattc cacaaaacgt agttctacac 3600 agtggatctc ttgtattgaa gccaaatacc gagcttcatg ttatttcttt tgagcagaaa 3660 gaaggctctt ctctcgttat gacacctgga tctgttcttt cgaaccagac tgttgctgat 3720 ggagctttgg tcataaataa catgaccatt gatttatcca gcgtagagaa aaatggtatt 3780 gctgaaggaa atatctttac tcctccagaa ttgagaatca tagacactac tacaggtgga 3840 agcggtggaa ccccatctac agatagtgaa agtaaccaga atagtgatga taccgaggag 3900 caaaataata atgacgcctc gaatcaagga gaaagcgcga atggatcgtc ttctcctgca 3960 gtagctgctg cacacacatc tcgtacaaga aactttgccg ctgcagctac agccacacct 4020 acgacaacac caacggctac aactacaaca agcaaccaag taatcctagg aggagaaatt 4080 aaactcatcg atcctaatgg gaccttcttc cagaaccctg cattaagatc cgaccaacaa 4140 atctccttgt tagtgctccc tacagactca tcaaaaatgc aagctcagaa aatagtactg 4200 acgggtgata ttgctcctca gaaaggatat acaggaacac tcactctgga tcctgatcaa 4260 ctacaaaatg gaacgatctc agtgctctgg aaatttgact cttatagaca atgggcttat 4320 gtacctagag acaatcattt ctatgcgaac tcgattctgg gatctcaaat gttaatggtc 4380 acagtcaaac aaggcttgct caacgataaa atgaatctag ctcgctttga ggaagttagc 4440 tataacaacc tgtggatatc aggactagga acgatgctat cgcaagtagg aacacctact 4500 tctgaagaat tcacttatta cagcagagga gcttctgttg ccttagatgc taaaccagcc 4560 catgatgtga ttgttggagc tgcatttagt aagatgatcg ggaaaacaaa atccttgaaa 4620 agagagaata actacactca caaaggatcc gaatattctt accaagcatc ggtatacgga 4680 ggcaaaccat tccactttgt aatcaataaa aaaacggaaa aatcgctacc gctattgtta 4740 caaggagtca tctcttacgg atatatcaaa catgatacag tgactcacta tccaacgatc 4800 cgtgaacgaa acaaaggaga atgggaagac ttaggatggc tgacagctct ccgtgtctcc 4860 tctgtcttaa gaactcctgc acaaggggat actaaacgta tcactgttta cggagaattg 4920 gaatactcca gtatccgtca gaaacaattc acagaaacag aatacgatcc tcgttacttc 4980 gacaactgca cctatagaaa cttagcaatt cctatggggt tagcattcga aggagagctc 5040 tctggtaacg atattttgat gtacaacaga ttctctgtag catacatgct atcaatctat 5100 cgaaattctc caacatgcaa ataccaagtg ctctcttcag gagaaggcgg agaaattatt 5160 tgtggagtac cgacaagaaa ctcagctcgc ggagaataca gcacgcagct gtacctggga 5220 cctttgtgga ctctgtatgg atcctacacg atagaagcag acgcacatac actagctcat 5280 atgatgaact gcggtgctcg tatgacattc taa 5313 23 408 PRT Chlamydia trachomatis 23 Met Met Lys Pro Leu Arg Phe Gly Tyr Phe Phe Cys Ala Ile Tyr Phe 1 5 10 15 Thr Leu Leu Gln Ala Ala Phe Ala Lys Glu Pro Asn Ser Cys Pro Asp 20 25 30 Cys Gln Asn Asn Trp Lys Glu Val Thr His Thr Asp Gln Leu Pro Glu 35 40 45 Asn Ile Ile His Ala Asp Asp Ala Cys Tyr His Ser Gly Tyr Val Gln 50 55 60 Ala Leu Ile Asp Met His Phe Leu Asp Ser Cys Cys Gln Val Ile Val 65 70 75 80 Glu Asn Gln Thr Ala Tyr Leu Phe Ser Leu Pro Thr Asp Asp Val Thr 85 90 95 Arg Asn Ala Ile Ile Asn Leu Ile Lys Asp Leu Pro Phe Ile His Ser 100 105 110 Val Glu Ile Cys Gln Ala Ser Tyr Gln Thr Cys His His Gln Gly Pro 115 120 125 His Gly Lys Thr Ser Leu Pro Glu Gln Arg Ser Phe Cys Thr Lys Val 130 135 140 Cys Gly Lys Glu Ala Ile Trp Leu Pro Gln Asn Thr Ile Leu Phe Ser 145 150 155 160 Pro Leu Val Ala Asp Pro Arg Gln Ala Thr Asn Ser Ala Gly Ile Arg 165 170 175 Phe Asn Asp Glu Val Leu Gly Lys Arg Val Gly Ser Ala Thr Phe Gly 180 185 190 Gly Asp Phe Ile Phe Leu Arg Leu Phe Asp Ile Ser Arg Phe His Gly 195 200 205 Asp Met Asp Ile Gly Leu Gln Gly Ala Val Phe Ser Val Phe Asp Leu 210 215 220 Asp His Pro Glu Ala Cys Met Val Asn Ser Asp Phe Phe Val Ala Ala 225 230 235 240 Leu Cys Asn Phe Ala Val Asn Lys Trp Ser Tyr Arg Phe Arg Leu Trp 245 250 255 His Leu Ser Ser His Leu Gly Asp Glu Phe Ile Leu Ala Asn Gln Leu 260 265 270 Pro Pro Lys Lys Arg Tyr Asn Arg Ser Asp Glu Ala Val Asp Phe Phe 275 280 285 Ala Ser Phe Arg Tyr Thr Pro Gln Ile Arg Val Tyr Gly Gly Ile Gly 290 295 300 Tyr Ile Ile Ser Arg Asp Leu Thr Phe Pro Glu Asp Pro Leu Tyr Phe 305 310 315 320 Glu Gly Gly Ile Glu Leu Arg Pro Phe Gly Leu Arg Glu Asp Asn Leu 325 330 335 His Ala Gln Pro Val Phe Ala Met His Phe Arg Phe Trp Glu Glu His 340 345 350 Asp Phe Ser Ile Asp Gln Thr Tyr Ile Val Gly Met Glu Trp Ser Lys 355 360 365 Phe Gln Asp Val Gly Arg Lys Val Arg Ala Val Leu Glu Tyr His Gln 370 375 380 Gly Phe Ser His Glu Gly Gln Phe Val Arg Glu Glu Cys Asp Tyr Tyr 385 390 395 400 Gly Phe Arg Leu Ser Tyr Gly Phe 405 24 1227 DNA Chlamydia trachomatis 24 atgatgaaac ctctacgttt cggttatttc ttttgcgcaa tctattttac tttgttacag 60 gcagcgtttg ctaaagaacc gaattcttgt cccgactgcc agaataattg gaaagaagtc 120 acccacacgg atcaactccc agaaaacatc attcatgctg atgatgcttg ttatcactct 180 ggttatgtac aggctctcat tgatatgcat ttcttagata gctgctgcca ggtcatcgtt 240 gaaaaccaaa ctgcttactt attttctctt cctacagatg atgttacgcg caacgccatt 300 atcaacctaa ttaaagacct tccattcatt cactccgtag aaatctgcca agcatcctat 360 caaacctgtc atcatcaagg ccctcatgga aagacttctc ttccagaaca acgttctttc 420 tgtacaaagg tctgtggaaa agaagctatt tggttaccac agaataccat cctattctcg 480 cctcttgtag cagatcctag acaagcaact aatagtgcag gtatccgttt taacgacgaa 540 gtcttaggaa aacgtgttgg ctctgctacc ttcggtggag atttcatctt cttacgatta 600 tttgatatct cccgattcca tggagacatg gatattggtc tccaaggagc tgtattctct 660 gttttcgacc tggatcatcc agaagcttgc atggtcaact ctgacttttt tgtcgccgct 720 ttgtgcaact ttgcagtgaa caaatggagc taccgcttca gactatggca tctttcttct 780 catcttggcg acgaatttat tcttgccaac cagttacctc ctaaaaaacg ttataatcga 840 agcgatgaag ccgtcgattt ctttgcttct tttcgttaca ctccacagat ccgtgtttat 900 ggaggtattg ggtatatcat tagtcgagat ttaacattcc ctgaagatcc tctttacttt 960 gaaggaggta tcgaactacg tcctttcgga ttacgggaag acaaccttca tgcccaaccc 1020 gtctttgcta tgcattttcg cttttgggaa gagcatgact tttctataga ccaaacttat 1080 atagtaggca tggagtggtc caaattccag gatgtaggga gaaaagtgcg cgctgtattg 1140 gaataccacc aaggtttctc ccacgaagga caatttgtcc gagaagaatg cgattattat 1200 ggctttcgat taagttatgg cttctag 1227 25 792 PRT Chlamydia trachomatis 25 Met Leu Gly Ile Arg Lys Lys Thr Ile Leu Gln Leu Ala Val Leu Leu 1 5 10 15 Leu Leu Thr Phe Ser Arg Ser Ser Phe Cys Ser Thr Ser Glu Gly Arg 20 25 30 Met Val Val Glu Ser Ile Thr Ile Thr Thr Gln Gly Glu Asn Thr Gln 35 40 45 Asn Lys Arg Ala Ile Pro Lys Ile Lys Thr Lys Gln Gly Thr Leu Phe 50 55 60 Ser Gln Ala Asp Phe Asp Glu Asp Leu Arg Thr Leu Ser Lys Asp Phe 65 70 75 80 Asp Arg Val Glu Pro Ile Val Glu Phe Arg Asn Gly Gln Ala Val Ile 85 90 95 Ser Leu Ile Leu Thr Ala Lys Pro Val Ile Arg Glu Ile Asn Ile Ser 100 105 110 Gly Asn Glu Ala Ile Pro Thr His Lys Ile Leu Lys Thr Leu Glu Leu 115 120 125 Tyr Lys Asn Asp Leu Phe Asp Arg Glu Leu Phe Phe Lys Asn Phe Asp 130 135 140 Ala Leu Arg Thr Leu Tyr Leu Lys Arg Gly Tyr Tyr Asp Ser Gln Leu 145 150 155 160 Ser Tyr Ser His Asn His Asn Glu Lys Glu Gly Phe Ile Asp Ile Ser 165 170 175 Ile Glu Ile Lys Glu Gly Arg His Gly Arg Ile Lys Lys Leu Thr Ile 180 185 190 Ser Gly Ile Thr Arg Thr Glu Ala Ser Asp Leu Gly Asp Ile Val Leu 195 200 205 Thr Lys Gln Tyr Ser Thr Thr Thr Ser Trp Phe Thr Gly Ala Gly Val 210 215 220 Tyr His Pro Asp Met Val Glu Gln Asp Leu Phe Ala Ile Thr Asn Tyr 225 230 235 240 Phe Gln Asn Lys Gly Tyr Ala Asp Ala Lys Val Ser Lys Glu Val Ser 245 250 255 Thr Asp Ala Lys Gly Asn Ile Thr Leu Leu Ile Val Val Asp Lys Gly 260 265 270 Pro Leu Tyr Thr Leu Gly His Val His Ile Glu Gly Phe Thr Ala Leu 275 280 285 Ser Lys Arg Leu Leu Asp Lys Gln Leu Leu Val Gly Pro Asn Ser Leu 290 295 300 Tyr Cys Pro Asp Lys Ile Trp Thr Gly Ala Gln Lys Ile Arg Ser Ala 305 310 315 320 Tyr Ala Arg Tyr Gly Tyr Val Asn Thr Asn Val Asp Val Ser Phe Ser 325 330 335 Ala His Pro Thr Leu Pro Val Tyr Asp Val Thr Tyr Arg Val Ser Glu 340 345 350 Gly Ser Pro Tyr Lys Ile Gly Leu Ile Lys Ile Lys Gly Asn Thr His 355 360 365 Thr Lys His Asp Val Ile Leu His Glu Thr Ser Leu Phe Pro Gly Asp 370 375 380 Thr Phe Asp Arg Leu Lys Leu Glu Gly Thr Glu Thr Arg Leu Arg Asn 385 390 395 400 Thr Gly Tyr Phe Lys Ser Val Ser Val Tyr Thr Val Arg Ser Gln Leu 405 410 415 Asp Pro Leu Asp Ser Asn Asp Leu Tyr Arg Asp Val Phe Ile Glu Val 420 425 430 Lys Glu Thr Glu Thr Gly Asn Leu Gly Leu Phe Leu Gly Phe Ser Ser 435 440 445 Ile Asp His Leu Phe Gly Gly Ala Glu Ile Ala Glu Ser Asn Phe Asp 450 455 460 Leu Phe Gly Ala Arg Asn Phe Leu Lys Lys Gly Phe Lys Ser Leu Arg 465 470 475 480 Gly Gly Gly Glu Tyr Leu Phe Leu Lys Ala Asn Leu Gly Asp Lys Val 485 490 495 Thr Asp Tyr Thr Val Lys Trp Thr Lys Pro His Phe Leu Asn Thr Pro 500 505 510 Trp Ile Leu Gly Val Glu Leu Asp Lys Ser Ile Asn Lys Ala Leu Ser 515 520 525 Lys Asp Tyr Ser Val Asp Thr Tyr Gly Gly Asn Ile Ser Thr Thr Tyr 530 535 540 Ile Leu Asn Asp Lys Leu Lys Tyr Gly Met Tyr Tyr Arg Gly Ser Gln 545 550 555 560 Thr Ser Leu Ser Leu Arg Lys Lys Thr Ser Ser Ser Asn Arg Pro Gly 565 570 575 Pro Asp Leu Asp Ser Asn Lys Gly Phe Val Ser Ala Ala Gly Leu Asn 580 585 590 Val Leu Tyr Asp Ser Ile Asp Asn Pro Arg Lys Pro Thr Met Gly Ile 595 600 605 Arg Ser Ser Leu Asn Phe Glu Leu Ser Gly Leu Gly Gly Thr Tyr Gln 610 615 620 Phe Thr Lys Leu Thr Ala Ser Gly Ser Ile Tyr Arg Leu Leu Thr Lys 625 630 635 640 Lys Gly Val Leu Lys Val Arg Ala Glu Ala Lys Phe Ile Lys Pro Phe 645 650 655 Gly Thr Thr Thr Ala Gln Gly Ile Pro Val Ser Glu Arg Phe Phe Leu 660 665 670 Gly Gly Glu Thr Thr Val Arg Gly Tyr Lys Pro Phe Ile Ile Gly Pro 675 680 685 Lys Phe Ser Pro Thr Glu Pro Gln Gly Gly Leu Ser Ser Leu Leu Leu 690 695 700 Thr Glu Glu Phe Gln Tyr Pro Leu Ile Ser Gln Pro Cys Ile Asn Ala 705 710 715 720 Phe Val Phe Leu Asp Ser Gly Phe Ile Gly Ile Glu Glu Tyr Thr Ile 725 730 735 Arg Leu Lys Asp Leu Cys Ser Ser Ala Gly Phe Gly Leu Arg Phe Asp 740 745 750 Met Met Asn Asn Val Pro Ile Met Leu Gly Trp Gly Trp Pro Phe Arg 755 760 765 Pro Thr Glu Ile Leu Asn Asn Glu Lys Ile Asp Val Ser Gln Arg Phe 770 775 780 Phe Phe Ala Leu Gly Gly Val Phe 785 790 26 2379 DNA Chlamydia trachomatis 26 atgcttggaa tacgcaaaaa aacgattctg caactcgctg ttttactgtt gctcaccttt 60 tcacgaagtt ctttctgttc aacttcagaa ggacgtatgg tcgtagagtc tatcaccatt 120 acgactcaag gagagaatac tcaaaataaa cgagctattc ctaaaataaa aacaaagcag 180 gggacgttgt tctctcaagc agattttgat gaagatctaa gaacactttc gaaagatttt 240 gatcgagtag agcctatcgt agagtttcgt aatggacaag ctgtgatctc tctgattctg 300 acggcaaaac ctgttatcag agagatcaat atttcaggaa atgaagctat ccccactcat 360 aaaattctga aaactttaga gctttataaa aatgatcttt ttgatcggga attattcttt 420 aaaaattttg atgcgctaag aactctttat ttgaaacgag ggtactacga ttctcaactc 480 tcctattctc ataatcataa tgagaaagag ggctttatcg atatttccat cgagattaaa 540 gaaggacgtc acggtcgcat aaaaaaatta acgatttcgg gaattacgcg aacagaagca 600 tcagacttag gtgacattgt tttaactaaa caatactcca caacaacgag ctggttcact 660 ggtgccggag tgtatcatcc ggacatggta gagcaagact tatttgctat cacaaattac 720 ttccaaaata aaggatatgc tgatgctaaa gtaagcaaag aggtctctac agatgctaaa 780 ggaaacatta ctttgcttat cgttgtagac aaaggacctt tatacacatt aggtcacgta 840 catatagaag gattcacagc gttatccaaa agactgctcg ataaacaact attggttgga 900 cctaactcct tatattgccc agataaaatt tggactggag cacaaaagat tcgtagcgca 960 tacgctagat atggctacgt gaacactaac gttgatgtct ccttctcagc gcaccccact 1020 ctacctgttt acgatgttac ctatcgagtg agtgaaggat ctccctacaa aatcgggtta 1080 attaaaatca aagggaacac tcatactaag catgatgtga ttttgcatga gactagtctt 1140 ttccctggag acacttttga tagattaaaa ctggaaggta cagagactcg tttacgcaac 1200 accggctact ttaaaagtgt aagtgtctat acggttcgtt cccaattaga tcctcttgat 1260 tctaacgacc tttatcgaga tgtttttatt gaagtcaaag agactgaaac aggaaatctt 1320 gggctattct taggattcag ctccattgac catttatttg gaggggcaga aattgcagaa 1380 agcaactttg atttatttgg agcccgaaac tttctcaaaa aaggattcaa atctttaaga 1440 ggtggtggag aatacctctt cctaaaagct aatttaggag ataaggtcac cgattacact 1500 gttaaatgga cgaaaccaca cttcttaaat accccttgga ttcttggagt agaattagat 1560 aaatcaatta ataaagcttt atcaaaagac tactctgtgg atacctatgg agggaatatc 1620 agtaccacct acattcttaa cgataagtta aaatatggga tgtattaccg tggtagccaa 1680 acaagcttaa gtttgcgcaa aaaaacgtcc agctctaata gacctggacc agatttagat 1740 agtaataaag gatttgtttc cgcagcggga ctcaatgttc tctatgattc tattgataat 1800 cctagaaaac ctactatggg aatccgcagc tccttaaact ttgaattatc tggtttaggc 1860 ggaacttacc aatttactaa actaacagct agtggttcta tctatcgctt attaactaaa 1920 aaaggtgttt tgaaagtccg tgcagaagct aagtttatca aacctttcgg aacaacaact 1980 gcacaaggca ttcctgtcag cgaacggttc ttcttaggag gtgaaaccac tgttcgcggt 2040 tacaaacctt ttattattgg accgaaattt tctcctactg aaccacaagg aggcttgtct 2100 tccctactat taacagaaga atttcaatat cctttgattt ctcaaccttg cattaatgcc 2160 tttgtatttc tagattccgg attcattggg atagaagagt acactattcg cctgaaagac 2220 ctttgcagta gcgccggatt tggtctacgc tttgatatga tgaataatgt gccaattatg 2280 ctaggctggg gttggccgtt ccgcccaaca gaaatcctca ataatgaaaa aattgatgta 2340 tctcaaagat tcttttttgc cttgggagga gtattctag 2379 27 254 PRT Chlamydia trachomatis 27 Met Phe Ala Phe Leu Thr Ser Lys Lys Val Gly Ile Leu Pro Ser Arg 1 5 10 15 Trp Gly Ser Ser Arg Phe Pro Gly Lys Pro Leu Ala Lys Ile Leu Gly 20 25 30 Lys Thr Leu Val Gln Arg Ser Tyr Glu Asn Ala Leu Ser Ser Gln Ser 35 40 45 Leu Asp Cys Val Val Val Ala Thr Asp Asp Gln Arg Ile Phe Asp His 50 55 60 Val Val Glu Phe Gly Gly Leu Cys Val Met Thr Ser Thr Ser Cys Ala 65 70 75 80 Asn Gly Thr Glu Arg Val Glu Glu Val Val Ser Arg His Phe Pro Gln 85 90 95 Ala Glu Ile Val Val Asn Ile Gln Gly Asp Glu Pro Cys Leu Ser Pro 100 105 110 Thr Val Ile Asp Gly Leu Val Ser Thr Leu Glu Asn Asn Pro Ala Ala 115 120 125 Asp Met Val Thr Ser Val Thr Glu Thr Thr Asp Pro Glu Ala Ile Leu 130 135 140 Thr Asp His Lys Val Lys Cys Val Phe Asp Lys Asn Gly Lys Ala Leu 145 150 155 160 Tyr Phe Ser Arg Ser Ala Ile Pro His Asn Phe Lys His Pro Thr Pro 165 170 175 Ile Tyr Leu His Ile Gly Val Tyr Ala Phe Arg Lys Ala Phe Leu Ser 180 185 190 Glu Tyr Val Lys Ile Pro Pro Ser Ser Leu Ser Leu Ala Glu Asp Leu 195 200 205 Glu Gln Leu Arg Val Leu Glu Ile Gly Arg Ser Ile Tyr Val His Val 210 215 220 Val Gln Asn Ala Thr Gly Pro Ser Val Asp Tyr Pro Glu Asp Ile Thr 225 230 235 240 Lys Val Glu Gln Tyr Leu Leu Cys Leu Ser Lys Ala Ser Phe 245 250 28 765 DNA Chlamydia trachomatis 28 gtgtttgcgt ttttaaccag caaaaaagtc ggcattctcc cctctagatg gggaagctcc 60 cgcttccccg gaaaacctct agcaaaaatt ctagggaaaa cccttgttca aagatcctat 120 gaaaatgcct taagcagtca atctctagat tgcgttgttg tggcaacaga tgatcaacga 180 atttttgacc atgtcgttga atttgggggg ctctgtgtca tgactagcac atcttgcgct 240 aacggaactg agcgagtaga agaggttgtg tctcgacatt ttcctcaagc agagattgtt 300 gtgaacatcc aaggagacga gccctgttta tctcctaccg tcatagatgg gcttgtgagc 360 acgctagaga acaatcctgc tgcagatatg gtcacatctg ttacagaaac aacagacccc 420 gaagcgatat tgacagatca caaagtgaag tgtgttttcg ataagaatgg caaagctctt 480 tactttagca gaagcgctat tcctcacaac tttaaacacc caacgcctat ttatctgcat 540 attggtgttt atgcttttag aaaagctttt ctaagtgaat atgttaaaat tcctccttcc 600 tcgttaagcc tagccgaaga tcttgaacaa ttacgagtat tagaaatagg tcgttctatc 660 tacgttcatg tcgttcagaa tgcaacgggc ccttctgttg attatcccga agatataacc 720 aaagtggagc agtatttatt atgtctttca aaagcatctt tttga 765 29 692 PRT Chlamydia trachomatis 29 Met Ile Arg Gln Trp Tyr Gly Phe Phe Leu Cys Leu Leu Phe Ser Tyr 1 5 10 15 Thr Ser Cys Phe Gly Val Glu Glu Asn Ser Gly Arg Ala Thr Pro Thr 20 25 30 Val Glu Leu Val Ser Glu Ser Glu Gln Ala Val Glu Gly Glu Val Leu 35 40 45 Arg Ile Gly Val Leu Ile Ala Ile Pro Glu Gly Glu His Ile Tyr Trp 50 55 60 Lys Asn Pro Gly Lys Leu Gly Met Pro Leu Arg Ile Ser Trp Asp Leu 65 70 75 80 Pro Ser Gly Cys Arg Leu Leu Glu Glu His Trp Pro Thr Pro Glu Ile 85 90 95 Phe Glu Glu Asp Gly Val Val Tyr Phe Gly Tyr Lys His Ser Thr Met 100 105 110 Val Val Ala Asp Ile Arg Val Ser Lys Glu Ile Glu Thr Arg Pro Leu 115 120 125 Glu Ile Lys Ala Gln Val Glu Trp Leu Ser Cys Gly Ala Ser Cys Leu 130 135 140 Pro Gly Ser Ser Ser Arg Val Leu Val Ile Pro Ile Asp Gln Gly Pro 145 150 155 160 Leu Ile Pro Asn Ser Lys Glu Thr Phe Thr Phe Ser Arg Ala Leu Ala 165 170 175 Ala Gln Pro Arg Pro Leu Asp Ala Ala Ile Lys Ile Ser Tyr Gln Pro 180 185 190 Asp Gly Leu Asp Val Phe Val Pro Ala Gly Lys Ala Asp Arg Ala Thr 195 200 205 Gln Ala Trp Phe Ile Ala Glu Asn Thr Arg Asp Phe Ala Tyr Ala Gln 210 215 220 Glu Val Pro Leu Glu Gln Ala Thr Thr Tyr Ile Trp Lys Leu Lys His 225 230 235 240 Pro Glu Gly Asn Met Pro Lys Gly Ile Gly Leu Ser Gly Ile Leu Ile 245 250 255 Phe Lys Asp Asp Ala Gly Lys Val Val Ala Ser Tyr Gln Val Glu Glu 260 265 270 Asn Gln Val Glu Gln Leu Ser Ala Leu Ser Trp Arg Phe Leu Ser Ile 275 280 285 Leu Leu Met Ala Phe Ile Gly Gly Ile Leu Leu Asn Ile Met Pro Cys 290 295 300 Val Leu Pro Leu Ile Thr Leu Lys Val Phe Ser Leu Ile Lys Ser Ala 305 310 315 320 Ala Asp His His Ser Ser Ser Val Ile Gly Gly Ile Gly Phe Thr Leu 325 330 335 Gly Ala Ile Val Ser Phe Trp Gly Leu Ala Phe Cys Ala Phe Leu Leu 340 345 350 Lys Val Leu Gly Gln Asn Ile Gly Trp Gly Phe Gln Leu Gln Glu Pro 355 360 365 Met Phe Val Ala Val Leu Ile Ile Val Phe Phe Leu Phe Ala Leu Ser 370 375 380 Ser Leu Gly Val Phe Glu Met Gly Ile Ile Cys Leu Ser Leu Gly Lys 385 390 395 400 Lys Leu Gln Glu Glu Gly Gly Ala Ser Val Arg Lys Asn Gln Ile Trp 405 410 415 Gly Ala Phe Phe Asn Gly Met Leu Thr Thr Leu Val Thr Thr Pro Cys 420 425 430 Thr Gly Pro Phe Leu Gly Ser Val Phe Gly Leu Val Met Ala Val Ser 435 440 445 Phe Val Lys Gln Leu Ala Ile Phe Thr Ala Ile Gly Leu Gly Met Ala 450 455 460 Ser Pro Tyr Leu Leu Phe Ala Ser Phe Pro Lys Met Leu Ala Ile Leu 465 470 475 480 Pro Lys Pro Gly Pro Trp Met Ser Thr Phe Lys Gln Leu Thr Gly Phe 485 490 495 Met Leu Leu Ala Thr Ala Thr Trp Leu Ile Trp Ile Phe Gly Val Glu 500 505 510 Thr Ser Ala Thr Ala Val Thr Ile Leu Leu Val Gly Leu Trp Leu Ala 515 520 525 Ala Val Gly Ala Trp Ile Leu Gly Arg Trp Gly Thr Leu Val Ser Pro 530 535 540 Arg Asn Gln Arg Leu Leu Ala Ser Val Val Phe Ile Phe Cys Ile Leu 545 550 555 560 Ser Ser Leu Val Ile Thr Ser Ile Gly Val Arg Tyr Phe Asp Glu Asn 565 570 575 Val Pro Pro Ala His Ser Ser Asp Trp Gln Ser Phe Ser Pro Glu Lys 580 585 590 Leu Ala Asp Leu Arg Glu Lys Gly Ile Pro Val Phe Val Asn Phe Thr 595 600 605 Ala Lys Trp Cys Leu Thr Cys Gln Leu Asn Lys Pro Leu Leu His Ala 610 615 620 Asn Met Gln Ala Phe Ala Ala Lys Gly Val Val Thr Leu Glu Ala Asp 625 630 635 640 Trp Thr Lys Lys Asp Pro Lys Ile Thr Glu Glu Leu Ala Arg Leu Gly 645 650 655 Arg Ala Ser Val Pro Ser Tyr Val Tyr Tyr Pro Ala Gly Asn Lys Ala 660 665 670 Pro Leu Ile Leu Pro Glu Arg Leu Ser Gln Ser Ala Leu Glu Glu Met 675 680 685 Val Phe Ser Gln 690 30 2079 DNA Chlamydia trachomatis 30 atgattcggc aatggtatgg attttttctt tgcttgctgt tcagctatac gtcttgtttt 60 ggtgtagaag aaaatagtgg aagagctacg cctacagtag aacttgttag tgaaagcgaa 120 caagctgttg aaggagaagt gcttcgtatc ggagtattga ttgctattcc agaaggagag 180 catatctact ggaaaaatcc agggaagctt ggaatgcctt tgcgcatttc ttgggatttg 240 ccatcaggat gtaggttgct ggaggaacat tggccgactc cagagatttt cgaagaggat 300 ggggttgttt attttggtta taaacattct acaatggtgg ttgcggatat tcgcgtttct 360 aaagagatag aaacgcgtcc attggagata aaagcgcaag ttgaatggtt gtcttgcggt 420 gcatcttgtc tcccaggttc ttcgtcaagg gttcttgtga ttcctataga tcaggggccg 480 ttaattccta atagtaaaga gacattcact ttttcccgtg cgttagcggc tcaacctcga 540 cctttggatg ctgccataaa gatttcttat cagcctgatg gcttagatgt ttttgtgcca 600 gcagggaaag cggatcgggc aacccaggca tggttcattg ctgaaaacac gcgagatttt 660 gcttatgctc aagaggttcc tcttgagcaa gcgactacgt acatatggaa gttgaaacat 720 cctgaaggaa atatgcctaa gggtattggg ttgtcgggga ttcttatatt caaggatgat 780 gcagggaaag tagtcgcttc gtatcaagta gaagagaatc aagtcgaaca gctttcggca 840 ttgagctgga ggtttctctc tattcttctt atggctttca ttggtggaat cttattaaac 900 atcatgccct gtgtattgcc tctgattact ttgaaagtat ttagtttaat taaatcggcg 960 gcagatcacc attcttcctc tgtgattgga gggattgggt ttactttagg ggctattgta 1020 agcttttggg gactcgcttt ttgtgcgttt ttgttaaagg ttttagggca aaatattgga 1080 tggggattcc agcttcaaga acccatgttt gttgccgttt taattattgt cttcttctta 1140 tttgctctga gttcgttagg cgtttttgag atgggaataa tttgtctgag cctagggaaa 1200 aaattgcaag aagagggagg ggcatcggta aggaagaatc agatctgggg agcttttttc 1260 aatgggatgt tgactaccct ggttacaact ccttgcactg ggccttttct tggctctgta 1320 tttggattag ttatggcagt gtcttttgtt aagcagctgg caatttttac tgctatagga 1380 ttaggaatgg caagtcccta tctattattt gcttcttttc cgaagatgct agccatttta 1440 cctaaacctg gtccttggat gagtacgttt aaacagttga ctgggtttat gttgcttgct 1500 actgcaactt ggcttatctg gatttttggg gtagagacga gtgcaaccgc tgtaactatt 1560 cttcttgtag gattgtggtt ggctgctgta ggtgcatgga ttctagggag atggggaacc 1620 cttgtatctc cgcgtaatca gcggcttctt gcttccgttg tattcatttt ctgtatttta 1680 agttccttag tgattacctc tataggtgtc cgttattttg atgagaacgt ccctcctgca 1740 catagctctg attggcaatc tttttctccc gaaaagctag ctgatttacg cgaaaaaggg 1800 attccagttt ttgtaaattt cactgcaaag tggtgtttaa cgtgtcaact caataagcct 1860 cttcttcatg ccaatatgca agcttttgct gctaagggcg tagttacttt agaagcagat 1920 tggacgaaaa aagatccaaa aattacagaa gaactcgctc gtttaggccg agccagtgta 1980 ccttcttatg tgtattaccc tgcggggaac aaagctccgc ttattcttcc agaaagatta 2040 tcgcaatctg ctttggaaga gatggttttt tctcagtag 2079 31 326 PRT Chlamydia trachomatis 31 Met Phe Arg Tyr Thr Leu Ser Arg Ser Leu Phe Phe Ile Leu Ala Leu 1 5 10 15 Phe Phe Cys Ser Ala Cys Asp Ser Arg Ser Met Ile Thr His Gly Leu 20 25 30 Ser Gly Arg Asp Ala Asn Glu Ile Val Val Leu Leu Val Ser Lys Gly 35 40 45 Val Ala Ala Gln Lys Val Pro Gln Ala Ala Ser Ser Thr Gly Gly Ser 50 55 60 Gly Glu Gln Leu Trp Asp Ile Ser Val Pro Ala Ala Gln Ile Thr Glu 65 70 75 80 Ala Leu Ala Ile Leu Asn Gln Ala Gly Leu Pro Arg Met Lys Gly Thr 85 90 95 Ser Leu Leu Asp Leu Phe Ala Lys Gln Gly Leu Val Pro Ser Glu Met 100 105 110 Gln Glu Lys Ile Arg Tyr Gln Glu Gly Leu Ser Glu Gln Met Ala Thr 115 120 125 Thr Ile Arg Lys Met Asp Gly Ile Val Asp Ala Ser Val Gln Ile Ser 130 135 140 Phe Ser Pro Glu Glu Glu Asp Gln Arg Pro Leu Thr Ala Ser Val Tyr 145 150 155 160 Ile Lys His Arg Gly Val Leu Asp Asn Pro Asn Ser Ile Met Val Ser 165 170 175 Lys Ile Lys Arg Leu Val Ala Ser Ala Val Pro Gly Leu Cys Pro Glu 180 185 190 Asn Val Ser Val Val Ser Asp Arg Ala Ser Tyr Ser Asp Ile Thr Ile 195 200 205 Asn Gly Pro Trp Gly Leu Ser Asp Glu Met Asn Tyr Val Ser Val Trp 210 215 220 Gly Ile Ile Leu Ala Lys His Ser Leu Thr Lys Phe Arg Leu Val Phe 225 230 235 240 Tyr Phe Leu Ile Leu Leu Leu Phe Ile Leu Ser Cys Gly Leu Leu Trp 245 250 255 Val Ile Trp Lys Thr His Thr Leu Ile Ser Ala Leu Gly Gly Thr Lys 260 265 270 Gly Phe Phe Asp Pro Ala Pro Tyr Ser Gln Leu Ser Phe Thr Gln Asn 275 280 285 Lys Pro Ala Pro Lys Glu Thr Pro Gly Ala Ala Glu Gly Ala Glu Ala 290 295 300 Gln Thr Ala Ser Glu Gln Pro Ser Lys Glu Asn Ala Glu Lys Gln Glu 305 310 315 320 Glu Asn Asn Glu Asp Ala 325 32 981 DNA Chlamydia trachomatis 32 atgtttcgtt atactctttc tcgatcctta tttttcattt tggctctttt cttctgctcg 60 gcttgtgata gtcgttccat gattacacac ggcttgtcag gacgtgatgc taatgaaatc 120 gtagtgcttc tagtcagtaa aggggtcgct gcacagaaag ttccccaagc agcgtcctca 180 acaggaggat ctggagaaca actctgggat atttcggttc ctgcagcaca aattacagag 240 gctctagcta ttctgaacca agctgggctt ccaagaatga aaggaaccag ccttcttgat 300 ctattcgcta aacaagggct ggtcccttct gaaatgcaag aaaaaatccg ctaccaagaa 360 ggtctttcag aacaaatggc tacgaccatt agaaagatgg acggtatcgt cgatgcgagc 420 gtacagattt ccttttctcc tgaagaagaa gatcaacggc cgctaacagc ctctgtatat 480 atcaaacaca gaggggtatt agacaaccct aacagtatta tggtgtctaa gattaaacgt 540 ttagttgcga gtgctgtccc aggactatgt cccgagaacg tttccgtagt cagtgaccga 600 gcttcttata gtgacattac tattaatggc ccttggggac tctccgatga aatgaattat 660 gtttctgtat gggggatcat tctagctaag cattccctta ctaaattccg ccttgttttc 720 tatttcttaa ttctccttct cttcattctt tcctgtgggc tactctgggt catttggaaa 780 acacacacac tgatttctgc tctgggtgga acaaaaggat tctttgatcc tgctccttac 840 tcacagctct ctttcactca gaataagcca gctccaaaag aaactcctgg agcagcagaa 900 ggtgcagaag cgcaaaccgc ttcggaacaa ccctctaaag aaaacgcaga aaaacaagaa 960 gagaataacg aggacgctta a 981 33 200 PRT Chlamydia trachomatis 33 Met Glu Lys Arg Gly Val Ile Val His Ile Leu Val Cys Leu Leu Thr 1 5 10 15 Ile Phe Gly Thr Phe Ser Leu Pro Ala Phe Gly Ala His Phe Leu Ala 20 25 30 Glu Glu Glu Gln Phe Tyr Met Asp Arg Phe Val Phe Ser Gly Gln Tyr 35 40 45 Pro Asp Met Glu Thr Met Glu Ile His Ala Glu Arg Lys Lys Arg Val 50 55 60 Gln Phe Asp Val Thr Gly Ser Phe Pro Lys Leu Glu Ser Val Val Tyr 65 70 75 80 Lys Gly Ser Phe Gly Leu Leu Arg Ser Lys Ile Lys Gly Glu Cys Pro 85 90 95 Glu Leu Ser Ser Val Asn Leu Ser Cys Thr Ser Cys Arg Met Asp Leu 100 105 110 Asp Phe Arg Gly Glu Trp Lys Lys Asn Ala Ser Ile Tyr Ile Arg Asn 115 120 125 Glu Gln Glu Pro Ile Thr Ile Met Leu Pro Lys Asp Ile Gly Val Val 130 135 140 Val Tyr Thr Gln Val Asp Met Asn Ser Lys Val Val Ala Glu Gly Ser 145 150 155 160 Leu Ile Lys Arg Gly Arg Gly Phe Trp Lys Lys Thr Phe Arg Asn Ser 165 170 175 Leu Val Gly Glu Ser Pro Val Thr Leu Thr Phe His Val Glu Thr Arg 180 185 190 Asn Gly Gly Val Ile Phe Leu Arg 195 200 34 603 DNA Chlamydia trachomatis 34 atggagaaga gaggcgttat tgtgcatata ctagtttgtt tgttgacaat cttcggaacg 60 ttcagtttac ccgctttcgg cgcgcatttt ctcgcggaag aagagcagtt ttatatggat 120 cggtttgttt tctctgggca gtatccagat atggaaacta tggaaatcca tgcagaaaga 180 aaaaaacgtg tacaatttga tgtgacggga agcttcccta agttggagag cgtggtttat 240 aagggatctt ttggattgct gcgttcgaaa ataaagggag agtgtccaga actgtcttct 300 gtaaatcttt cttgtacctc ctgcagaatg gatttagatt ttcgagggga gtggaaaaag 360 aatgcgtcta tttatattcg taatgagcaa gagccaatta caattatgtt gcctaaagac 420 attggtgtag ttgtctatac gcaggttgat atgaatagta aagtagttgc agagggatca 480 ctaatcaaga gaggaagagg tttttggaag aaaacttttc ggaattcttt ggtaggagaa 540 tcccctgtga cgctaacttt tcatgtagag actcgtaatg gaggagttat ttttctccgt 600 tag 603 35 305 PRT Chlamydia trachomatis 35 Met Phe Asp Ser Asp His Asn Ser Ile Phe Gln Ser Asp Leu Cys Gln 1 5 10 15 Arg Leu Val Val His Ser Ile Leu Leu Thr Phe Leu Val Ile Leu Leu 20 25 30 Cys Thr Ser Leu Tyr Pro Ser Ser Ala Phe Ile Val Gly Leu Leu Ser 35 40 45 Ser Ala Cys Ala Ala Leu Gly Thr Tyr Glu Met Gly Ala Met Val Arg 50 55 60 Ile Lys Phe Pro Phe Ser Phe Thr Arg Tyr Ser Ala Leu Gly Ser Ala 65 70 75 80 Ile Phe Ile Ala Leu Thr Cys Leu Thr Ala Arg Cys Lys Met Cys Phe 85 90 95 Pro Glu His Ile Asp Leu Leu Pro Trp Phe Phe Leu Phe Phe Trp Thr 100 105 110 Ile Arg Leu Val Phe Lys Ser Arg His Tyr Lys Leu Gly Pro Ile Gly 115 120 125 Ser Thr Gly Leu Ala Leu Phe Cys Met Leu Tyr Val Ser Val Pro Ile 130 135 140 Arg Leu Phe Leu His Ile Leu Tyr Gly Phe Val His Thr Asp Thr Pro 145 150 155 160 Phe Val Gly Ile Trp Trp Ala Ile Phe Leu Ile Ala Thr Thr Lys Ser 165 170 175 Ser Asp Ile Phe Gly Tyr Phe Phe Gly Lys Ala Phe Gly Lys Lys Arg 180 185 190 Ile Ala Pro Val Ile Ser Pro Asn Lys Thr Val Val Gly Phe Ile Ala 195 200 205 Gly Cys Cys Gly Ser Ile Leu Val Ser Leu Leu Phe Tyr Ser His Leu 210 215 220 Pro Lys Ala Phe Ala Asp Gln Ile Ala Val Pro Trp Ile Leu Ile Ala 225 230 235 240 Leu Gly Thr Val Leu Gly Val Ser Gly Phe Phe Gly Asp Ile Ile Glu 245 250 255 Ser Thr Phe Lys Arg Asp Ala Gln Ile Lys Asn Ser Ser Asp Leu Glu 260 265 270 Ser Ile Gly Gly Met Leu Asp Val Leu Asp Ser Leu Leu Leu Ser Thr 275 280 285 Pro Ile Val Tyr Ala Ile Leu Leu Ile Thr Gln Asn Arg Thr Phe Leu 290 295 300 Gly 305 36 918 DNA Chlamydia trachomatis 36 atgttcgatt cggatcataa ttccattttt caaagcgatt tgtgtcagcg tctggtcgtc 60 cactcgattc ttcttacttt ccttgtcatt cttctctgta catctttata tcccagctca 120 gcctttattg tagggcttct ttcctccgct tgcgcagctc taggaacata tgagatgggg 180 gctatggtta gaatcaagtt tccattttct ttcacacgct atagtgcatt aggatccgct 240 attttcattg ctctgacctg tcttacagct cgttgtaaaa tgtgttttcc agagcatata 300 gacctacttc cttggttctt tctcttcttt tggacgattc gcttagtatt taaaagtcgc 360 cattataaac ttggtcccat aggctcaact gggctcgcgt tgttttgtat gctttatgta 420 tcagtcccta tccgcttgtt cctccacatt ttgtatgggt ttgtgcatac cgatactcca 480 tttgtaggaa tttggtgggc gatttttctt atcgctacaa caaaaagctc tgatattttt 540 ggttacttct ttggaaaagc ttttgggaaa aaacgcattg caccagtcat tagcccgaac 600 aaaacagtag taggcttcat tgctggttgc tgtggatcta tcttggttag ccttcttttc 660 tactcccatc ttcctaaagc ctttgctgat cagattgcgg tgccttggat cctcattgct 720 ttaggtactg tgttgggcgt tagtggattc tttggagaca ttatcgaatc tacgttcaaa 780 cgggatgcac agatcaagaa cagcagtgat ctggagtcta tcggaggaat gctagatgtg 840 ctagactcct tgcttctttc gactcctatc gtttacgcta tcctccttat cactcaaaat 900 aggacatttt taggatga 918 37 621 PRT Chlamydia trachomatis 37 Met Arg Arg Ser Val Cys Tyr Val Thr Pro Ser Val Ala Arg Ala Gly 1 5 10 15 Gln Ile Ser Thr Trp Arg Phe Glu Tyr Ser Ser Ala Asn Phe Leu Pro 20 25 30 Glu Gly Thr Leu Leu Lys Phe Asp Leu Gly Ile Asp Gly Arg Pro Ile 35 40 45 Asp Trp Glu Ile Pro Ser Thr Asp Leu Ser Gln Pro Cys Asn Thr Ile 50 55 60 Tyr Leu Glu Thr Pro Ser Glu Asp Ile Val Ala Ala Lys Ala Val Tyr 65 70 75 80 Ala Pro Gly Gly Tyr Ile Pro Thr Phe Glu Phe Thr Leu Pro Cys Asp 85 90 95 Val Glu Ala Gly Asp Thr Phe Ser Ile Ile Leu Gly Ser Ser Pro Asn 100 105 110 Phe Pro Gln Glu Asp Ser Ser Gly Asn Gly Ala Gln Leu Phe Thr Gln 115 120 125 Arg Arg Lys Pro Phe Ser Leu Tyr Val Asp Pro Ser Gly Lys Gly Ser 130 135 140 Phe Glu Asp Pro Asp Ile Phe Thr Met Asp Ile Arg Gly Asn Val Leu 145 150 155 160 Lys Asn Ile Arg Ile Phe Ala Pro Ser Tyr Val Ile Lys Asn Lys Arg 165 170 175 Phe Asp Ile Thr Val Arg Phe Glu Asp Glu Phe Gly Asn Leu Thr Asn 180 185 190 Phe Ser Pro Glu Glu Thr His Ile Glu Leu Ser Tyr Glu His Leu Arg 195 200 205 Glu Asn Leu Asn Trp Gln Leu Phe Ile Pro Glu Thr Gly Phe Val Ile 210 215 220 Leu Pro Asn Leu Tyr Phe Asn Glu Pro Gly Ile Tyr Arg Ile Gln Leu 225 230 235 240 Arg Asn Gln Ala Thr Lys Glu Val Phe Thr Ser Ala Pro Ile Lys Cys 245 250 255 Phe Ala Glu Thr Ser Ser His Leu Leu Trp Gly Leu Leu His Gly Glu 260 265 270 Ser Glu Arg Val Asp Ser Glu Gly Asn Ile Glu Ser Cys Leu Arg Tyr 275 280 285 Phe Arg Asp Asp Cys Ala Leu Asn Phe Phe Ala Thr Ser Ser Phe Glu 290 295 300 Ile Gln Asp Gly Leu Thr Pro Glu Thr Ile Lys Thr Ile Asn Gln Thr 305 310 315 320 Val Ala Asp Phe Asn Glu Glu Asp Arg Phe Ile Ala Leu Ser Gly Ala 325 330 335 Gln Tyr Leu Ser Glu Glu Pro Gly Glu Gly Ile Arg Glu Val Leu Leu 340 345 350 Met Lys Glu Pro Lys Ser Pro Gly Lys His Lys Glu Cys Lys Leu Phe 355 360 365 Pro Leu Ser Lys Leu Tyr Lys Gln Ser Thr Ser His Glu Leu Ile Ser 370 375 380 Ile Pro Ser Phe Thr Ala Ser Lys Lys Phe Gly Tyr Asn Phe Asn Asn 385 390 395 400 Phe His Pro Glu Phe Glu Arg Val Val Glu Ile Tyr Asn Ala Trp Gly 405 410 415 Cys Ser Glu Arg Thr Glu Ala Glu Gly Asn Pro Phe Pro Ile Lys Gly 420 425 430 Ser Ile Asp Ser Glu Asn Pro Glu Gly Thr Val Leu Ser Ala Leu Lys 435 440 445 Arg Asn Leu Arg Phe Gly Phe Val Ala Gly Gly Leu Asp Asp Arg Asn 450 455 460 Leu Tyr Asn His Phe Phe Asp Ser Asp Gln Gln Gln Tyr Ser Pro Gly 465 470 475 480 Leu Thr Ala Val Ile Cys Asn Lys Tyr Ser Arg Asp Ser Leu Leu Glu 485 490 495 Ala Leu Tyr Gln Arg Gln Cys Tyr Ala Thr Thr Gly Gln Arg Ile Ile 500 505 510 Val Asn Phe Gln Ile Thr Ser Ala Pro Met Gly Ser Glu Leu Ser Thr 515 520 525 Ala Ile Lys Pro Gly Leu Val Ile Asn Arg His Ile Ser Gly Tyr Val 530 535 540 Ala Gly Thr Ala Lys Ile Ala Ser Ile Glu Ile Ile Arg Asn Glu Asp 545 550 555 560 Ile Leu His Thr Phe His Pro Asp Gly Asn Asn Phe Glu Tyr Glu Tyr 565 570 575 Asp Asp Leu Ser Pro Phe Ala Gln Val Thr Leu Lys Asp Pro Gln Asn 580 585 590 Gly Ala Pro Phe Ala Phe Tyr Tyr Leu Arg Val Thr Gln Glu Asn Gly 595 600 605 Ala Met Ala Trp Ser Ser Pro Ile Trp Ile Asp Leu Asn 610 615 620 38 1866 DNA Chlamydia trachomatis 38 atgcgcagat ctgtttgtta cgttactcct tcagttgcta gggctggtca aatttctacc 60 tggcgtttcg aatattcttc agctaatttc cttcccgaag gcacattgct aaaatttgac 120 ctgggaatag acggacgccc tatagactgg gagattcctt ctacagatct ttctcaacca 180 tgtaatacaa tttatttaga aacgccttcc gaggatattg tggctgcaaa agctgtgtat 240 gctcccggag gctatatccc tactttcgaa tttactctcc cttgtgatgt ggaagctggg 300 gacactttct ctattattct tggctcctct cccaacttcc ctcaagagga ctcttcaggt 360 aatggtgctc aattatttac tcaacgccgt aaacctttct ctctttatgt tgacccatca 420 gggaaaggat cttttgaaga tcccgatatc ttcacaatgg atatcagagg aaatgtatta 480 aaaaatatcc ggatttttgc tccttcttat gtgatcaaaa acaaacgctt tgatattaca 540 gtgcgcttcg aagatgaatt tgggaactta accaatttct ccccagaaga gacccatatc 600 gagctttcgt acgaacatct tcgcgaaaac ctcaattggc aattgttcat ccctgaaaca 660 ggctttgtga tccttccaaa cctgtatttc aatgaaccag gtatttatcg tattcaacta 720 cgcaatcaag caacaaaaga ggtctttaca tcagcgccta tcaaatgttt tgcagaaacc 780 tcctctcatc ttttgtgggg gcttctacat ggagaatctg aacgtgtcga ctctgaaggt 840 aatatcgagt cttgcttgcg ttattttcgt gatgactgcg cgttaaactt ttttgcaaca 900 tcctctttcg aaattcaaga tggcctgacc ccagaaacca ttaaaaccat taaccaaacc 960 gttgctgatt ttaatgaaga agatcgtttc attgccttat ccggagcaca gtacctttct 1020 gaagagcctg gcgagggaat tcgtgaagta ttgctgatga aggaacccaa atccccaggg 1080 aaacataaag aatgcaaact atttccttta tctaagctat ataagcaatc aactagtcat 1140 gagttaatct caatccccag cttcactgct tcaaagaaat ttggatacaa ttttaataat 1200 ttccatcctg aatttgaaag agttgttgaa atttataatg cctggggatg ctctgaaaga 1260 actgaagctg aaggaaaccc tttccctatt aaaggttcta tcgactcaga aaatccagag 1320 ggaactgttc tatctgcttt aaagagaaac ctgcgttttg gattcgtagc cggtggtctt 1380 gatgatagaa atctatacaa tcactttttt gattccgatc aacagcaata ctcccctgga 1440 ttaacagctg tgatctgcaa taaatattct cgggattcct tactcgaggc attataccaa 1500 cgacaatgct atgctacaac cggccaaaga attatcgtga atttccagat tacatctgct 1560 cctatgggct ccgaactctc cacagccatt aaaccagggc tcgtgatcaa tagacatatt 1620 tcgggatatg tagcaggaac tgccaagatt gcgtcgatcg aaatcatccg caatgaggat 1680 attctccata ccttccaccc agatggaaat aactttgagt atgagtacga cgatctctct 1740 ccttttgcac aagtcactct aaaagatcct caaaatggag ctccttttgc tttttactac 1800 ttacgagtca ctcaagagaa tggagctatg gcttggagct ctcctatttg gatagatctt 1860 aactaa 1866 39 697 PRT Chlamydia trachomatis 39 Met Lys Arg Phe Phe Pro Leu Phe Ile Gly Val Leu Leu Ala His Thr 1 5 10 15 Leu Pro Ser Glu Gly Leu Ser His Gln Gln Ala Val Gln Lys Lys Ile 20 25 30 Ser Tyr Leu Ser His Phe Lys Gly Ile Thr Gly Ile Met Asp Val Glu 35 40 45 Asp Gly Val Leu His Ile His Asp Asp Leu Arg Leu Gln Ala Asn Lys 50 55 60 Ala Tyr Val Glu Asn Arg Thr Asp Cys Gly Ile Lys Ile Val Ala His 65 70 75 80 Gly Asn Val Met Val Asn Tyr Arg Gly Lys Ile Leu Ile Cys Asp Tyr 85 90 95 Leu Glu Tyr Tyr Glu Asp Thr Asp Ser Cys Leu Leu Thr Asn Gly Arg 100 105 110 Cys Ser Leu Tyr Pro Trp Phe Ile Gly Gly Ser Thr Ile Thr Ile Ser 115 120 125 Pro Ser Ser Ile Ile Ile His Lys Gly Tyr Ile Ser Thr Ser Glu Gly 130 135 140 Pro Gln Lys His Ile Cys Leu Ser Gly Asp Tyr Leu Lys Tyr Ser Ser 145 150 155 160 Asp Ser Val Leu Ser Met Gly Pro Ser Arg Leu Ser Ile Cys Asn Thr 165 170 175 Pro Val Leu Leu Leu Pro Gln Ile Ser Ile Met Pro Met Glu Ile Pro 180 185 190 Lys Pro Pro Ile Thr Phe Arg Gly Gly Ser Gly Gly Phe Leu Gly Ser 195 200 205 Tyr Leu Gly Val Ser Tyr Ser Pro Ile Ser Lys Lys His Cys Ser Thr 210 215 220 Thr Leu Phe Leu Asp Gly Phe Phe Lys His Gly Ile Gly Leu Gly Tyr 225 230 235 240 Asn Met Arg Phe Ser Ser Gln Glu Asn Pro Ser Asn Ala Ile Asn Ile 245 250 255 Lys Ser Tyr Tyr Ala His Arg Leu Ala Ile Asp Ser Ser Gly Ala Lys 260 265 270 Asp Arg Tyr Arg Leu His Gly Asp Phe Thr Phe Ser Lys Glu Arg Ala 275 280 285 His Leu Ala Gly Glu Phe His Leu Ser Asp Ser Trp Glu Thr Val Val 290 295 300 Asp Ile Phe Pro Asn Asn Phe Ser Leu Lys Asn Thr Gly Pro Thr Glu 305 310 315 320 Val Ser Leu Ser Trp Arg Asp Asn Asn Leu Phe Gly Lys Met Thr Ser 325 330 335 Ser Val Lys Val Asn Ser Phe Gln Asn Val Lys Gln Glu Leu Pro Gln 340 345 350 Ala Ile Leu His His Arg Pro Val Arg Ile Arg Arg Ser Arg Ile Phe 355 360 365 Leu Glu Asn Arg Leu Glu Ala Gly Phe Leu Asp Phe His Phe Ser Ser 370 375 380 Asn Ile Pro Gly Ser Asn Phe Ser Ser Trp Arg Phe Ser Ser Ala His 385 390 395 400 Lys Val Tyr Arg Gly Leu Val Leu Pro Ile Gly Thr Leu Thr Pro Ser 405 410 415 Leu Ser Gly Thr Ala Ile Tyr Tyr Thr Arg Met Leu Ser Pro Asn Ala 420 425 430 Ala His Cys Gln Leu Ser Gly Ser Leu Ser Phe Asp Tyr Arg Val Ala 435 440 445 Leu Gln Lys Glu Tyr Arg His Ala Arg His Ile Val Glu Pro Phe Cys 450 455 460 Ser Phe Leu Lys Thr Thr Arg Pro Val Leu Ser Ser Asp Glu Pro His 465 470 475 480 Ile Phe Ser Ile Lys Asp Ala Phe His Ser Ile Asn Leu Leu His Val 485 490 495 Gly Leu Glu Ser Lys Val Leu Asn Lys His Ser Thr Pro Ser His Leu 500 505 510 Lys Leu Trp Thr Thr Tyr Ile Phe Asp Glu Pro His Ala Lys Asp Thr 515 520 525 Phe Pro Lys Thr Ala Cys Trp Phe Ser Leu Pro Leu Thr Leu Gln Asn 530 535 540 Thr Leu Ser Leu Asp Ala Glu Trp Ile Trp Lys Lys Ser Arg Trp Asp 545 550 555 560 His Leu Asn Val Ile Trp Glu Trp Ile Leu Asn Asp Asn Leu Gly Leu 565 570 575 Thr Leu Glu Phe Leu His Arg Ser Lys Tyr Gly Phe Ile Lys Cys Ala 580 585 590 Lys Asp Asn Tyr Thr Leu Asp Val Ser Arg Ser Leu Asp Thr Leu Leu 595 600 605 Ala Ser Pro Leu Ser Asp Arg Arg Asn Leu Ile Thr Gly Lys Leu Phe 610 615 620 Val Arg Pro His Pro His Trp Asn Tyr Asn Leu Asn Leu Arg Tyr Gly 625 630 635 640 Trp His Arg Pro Asp Ser Pro Ser Tyr Leu Glu Tyr Gln Met Ile Leu 645 650 655 Gly His Lys Ile Phe Glu His Trp Gln Leu Phe Ser Val Tyr Glu Lys 660 665 670 Arg Glu Ala Asp Lys Arg Cys Phe Phe Tyr Leu Lys Leu Asp Lys Arg 675 680 685 Lys Gln Lys His Arg His Pro Phe Gly 690 695 40 2094 DNA Chlamydia trachomatis 40 gtgaaacgat ttttcccact ttttattgga gtgctgctcg cgcacacttt gccgtcagaa 60 ggtctttctc atcaacaagc tgtccaaaaa aaaatttctt atctgagcca ttttaaaggc 120 attacaggaa ttatggatgt tgaggatggg gtattacata tccatgatga tctacgtctt 180 caagccaata aagcctatgt tgaaaatcgc acggattgtg ggatcaaaat cgttgctcat 240 ggcaacgtta tggtcaatta tcgcgggaaa attttaatct gtgattatct tgagtactat 300 gaagatacag attcttgttt actcaccaat ggccgctgtt cgttataccc atggttcatt 360 ggaggatcca ctataacgat ctcaccatct tctattatca ttcataaagg gtatatctcg 420 acttctgaag gtcctcagaa acatatttgt ttatccggag attatttaaa atactcttca 480 gacagcgtat tatctatggg accctcacgt ctttctatct gtaatacgcc tgtgttattg 540 cttcctcaaa tctccattat gcctatggag attcctaagc ctccgattac ttttcgaggt 600 gggagtggag gatttctggg atcctactta ggtgttagtt attcccctat atctaaaaag 660 cattgttcta cgactttgtt cttggatggt ttttttaaac atggaatagg tctcggctat 720 aacatgcgct tttcctctca ggaaaatcca agcaatgcca taaatattaa aagctattac 780 gcacatcgat tagctattga ttcatcagga gcaaaagatc gctatcgatt acatggagac 840 ttcacttttt ccaaagaacg agcccatctt gctggtgaat tccatttaag tgatagctgg 900 gaaacagttg tggatatctt cccaaataac ttctctttaa aaaatacagg ccctacagaa 960 gttagcctat catggcgcga taacaattta tttgggaaaa tgacttcctc tgtcaaagtc 1020 aactcctttc aaaatgttaa acaagaattg cctcaagcaa ttcttcatca ccgaccagta 1080 cgtatcaggc gctctcgcat tttcctagag aatcgcttag aagctggttt tttagatttt 1140 catttcagta gtaatattcc aggctctaac ttctcatcat ggaggttctc atccgctcac 1200 aaagtctacc gtgggcttgt tcttcctata ggaacgttaa ccccttcgct atctggaact 1260 gctatctact atacccgcat gctctcccca aatgcagccc attgtcaatt atctggatcg 1320 ctatcttttg attatcgcgt tgctttacaa aaagaatatc ggcatgcaag acatattgta 1380 gagccttttt gctccttttt aaaaaccact cgtcctgtat tatcctctga tgagcctcat 1440 attttctcga ttaaagatgc ttttcactct atcaaccttc tacatgtagg attggagtca 1500 aaagtcttaa acaaacattc cactccttcg catctgaaac tatggacgac ctatatcttt 1560 gatgaacctc acgctaagga cactttccct aaaactgctt gctggttctc tcttcctctt 1620 acactccaaa atactttatc cttagatgcg gaatggattt ggaaaaaaag ccgatgggat 1680 catctcaatg taatctggga atggattttg aatgataatc tcggtcttac tttagaattt 1740 ttacatagaa gtaagtatgg ctttattaag tgcgctaaag ataactacac actcgatgta 1800 agccgatctt tagacacatt actagcctct cctctttccg atcgaagaaa tttgattact 1860 ggcaaacttt ttgttcgtcc acatcctcat tggaattata atcttaatct tcgttatgga 1920 tggcatcgtc cagactctcc atcctattta gaataccaga tgattctggg tcataaaatc 1980 tttgagcact ggcagctatt ctctgtctac gaaaaacgtg aagctgataa gcgctgcttc 2040 ttttatctaa aattagataa acgaaaacag aaacaccgcc atccttttgg ataa 2094 41 1016 PRT Chlamydia trachomatis 41 Met Pro Phe Ser Leu Arg Ser Thr Ser Phe Cys Phe Leu Ala Cys Leu 1 5 10 15 Cys Ser Tyr Ser Tyr Gly Phe Ala Ser Ser Pro Gln Val Leu Thr Pro 20 25 30 Asn Val Thr Thr Pro Phe Lys Gly Asp Asp Val Tyr Leu Asn Gly Asp 35 40 45 Cys Ala Phe Val Asn Val Tyr Ala Gly Ala Glu Asn Gly Ser Ile Ile 50 55 60 Ser Ala Asn Gly Asp Asn Leu Thr Ile Thr Gly Gln Asn His Thr Leu 65 70 75 80 Ser Phe Thr Asp Ser Gln Gly Pro Val Leu Gln Asn Tyr Ala Phe Ile 85 90 95 Ser Ala Gly Glu Thr Leu Thr Leu Lys Asp Phe Ser Ser Leu Met Phe 100 105 110 Ser Lys Asn Val Ser Cys Gly Glu Lys Gly Met Ile Ser Gly Lys Thr 115 120 125 Val Ser Ile Ser Gly Ala Gly Glu Val Ile Phe Trp Asp Asn Ser Val 130 135 140 Gly Tyr Ser Pro Leu Ser Ile Val Pro Ala Ser Thr Pro Thr Pro Pro 145 150 155 160 Ala Pro Ala Pro Ala Pro Ala Ala Ser Ser Ser Leu Ser Pro Thr Val 165 170 175 Ser Asp Ala Arg Lys Gly Ser Ile Phe Ser Val Glu Thr Ser Leu Glu 180 185 190 Ile Ser Gly Val Lys Lys Gly Val Met Phe Asp Asn Asn Ala Gly Asn 195 200 205 Phe Gly Thr Val Phe Arg Gly Asn Ser Asn Asn Asn Ala Gly Ser Gly 210 215 220 Gly Ser Gly Ser Ala Thr Thr Pro Ser Phe Thr Val Lys Asn Cys Lys 225 230 235 240 Gly Lys Val Ser Phe Thr Asp Asn Val Ala Ser Cys Gly Gly Gly Val 245 250 255 Val Tyr Lys Gly Thr Val Leu Phe Lys Asp Asn Glu Gly Gly Ile Phe 260 265 270 Phe Arg Gly Asn Thr Ala Tyr Asp Asp Leu Gly Ile Leu Ala Ala Thr 275 280 285 Ser Arg Asp Gln Asn Thr Glu Thr Gly Gly Gly Gly Gly Val Ile Cys 290 295 300 Ser Pro Asp Asp Ser Val Lys Phe Glu Gly Asn Lys Gly Ser Ile Val 305 310 315 320 Phe Asp Tyr Asn Phe Ala Lys Gly Arg Gly Gly Ser Ile Leu Thr Lys 325 330 335 Glu Phe Ser Leu Val Ala Asp Asp Ser Val Val Phe Ser Asn Asn Thr 340 345 350 Ala Glu Lys Gly Gly Gly Ala Ile Tyr Ala Pro Thr Ile Asp Ile Ser 355 360 365 Thr Asn Gly Gly Ser Ile Leu Phe Glu Arg Asn Arg Ala Ala Glu Gly 370 375 380 Gly Ala Ile Cys Val Ser Glu Ala Ser Ser Gly Ser Thr Gly Asn Leu 385 390 395 400 Thr Leu Ser Ala Ser Asp Gly Asp Ile Val Phe Ser Gly Asn Met Thr 405 410 415 Ser Asp Arg Pro Gly Glu Arg Ser Ala Ala Arg Ile Leu Ser Asp Gly 420 425 430 Thr Thr Val Ser Leu Asn Ala Ser Gly Leu Ser Lys Leu Ile Phe Tyr 435 440 445 Asp Pro Val Val Gln Asn Asn Ser Ala Ala Gly Ala Ser Thr Pro Ser 450 455 460 Pro Ser Ser Ser Ser Met Pro Gly Ala Val Thr Ile Asn Gln Ser Gly 465 470 475 480 Asn Gly Ser Val Ile Phe Thr Ala Glu Ser Leu Thr Pro Ser Glu Lys 485 490 495 Leu Gln Val Leu Asn Ser Thr Ser Asn Phe Pro Gly Ala Leu Thr Val 500 505 510 Ser Gly Gly Glu Leu Val Val Thr Glu Gly Ala Thr Leu Thr Thr Gly 515 520 525 Thr Ile Thr Ala Thr Ser Gly Arg Val Thr Leu Gly Ser Gly Ala Ser 530 535 540 Leu Ser Ala Val Ala Gly Ala Ala Asn Asn Asn Tyr Thr Cys Thr Val 545 550 555 560 Ser Lys Leu Gly Ile Asp Leu Glu Ser Phe Leu Thr Pro Asn Tyr Lys 565 570 575 Thr Ala Ile Leu Gly Ala Asp Gly Thr Val Thr Val Asn Ser Gly Ser 580 585 590 Thr Leu Asp Leu Val Met Glu Ser Glu Ala Glu Val Tyr Asp Asn Pro 595 600 605 Leu Phe Val Gly Ser Leu Thr Ile Pro Phe Val Thr Leu Ser Ser Ser 610 615 620 Ser Ala Ser Asn Gly Val Thr Lys Asn Ser Val Thr Ile Asn Asp Ala 625 630 635 640 Asp Ala Ala His Tyr Gly Tyr Gln Gly Ser Trp Ser Ala Asp Trp Thr 645 650 655 Lys Pro Pro Leu Ala Pro Asp Ala Lys Gly Met Val Pro Pro Asn Thr 660 665 670 Asn Asn Thr Leu Tyr Leu Thr Trp Arg Pro Ala Ser Asn Tyr Gly Glu 675 680 685 Tyr Arg Leu Asp Pro Gln Arg Lys Gly Glu Leu Val Pro Asn Ser Leu 690 695 700 Trp Val Ala Gly Ser Ala Leu Arg Thr Phe Thr Asn Gly Leu Lys Glu 705 710 715 720 His Tyr Val Ser Arg Asp Val Gly Phe Val Ala Ser Leu His Ala Leu 725 730 735 Gly Asp Tyr Ile Leu Asn Tyr Thr Gln Asp Asp Arg Asp Gly Phe Leu 740 745 750 Ala Arg Tyr Gly Gly Phe Gln Ala Thr Ala Ala Ser His Tyr Glu Asn 755 760 765 Gly Ser Ile Phe Gly Val Ala Phe Gly Gln Leu Tyr Gly Gln Thr Lys 770 775 780 Ser Arg Met Tyr Tyr Ser Lys Asp Ala Gly Asn Met Thr Met Leu Ser 785 790 795 800 Cys Phe Gly Arg Ser Tyr Val Asp Ile Lys Gly Thr Glu Thr Val Met 805 810 815 Tyr Trp Glu Thr Ala Tyr Gly Tyr Ser Val His Arg Met His Thr Gln 820 825 830 Tyr Phe Asn Asp Lys Thr Gln Lys Phe Asp His Ser Lys Cys His Trp 835 840 845 His Asn Asn Asn Tyr Tyr Ala Phe Val Gly Ala Glu His Asn Phe Leu 850 855 860 Glu Tyr Cys Ile Pro Thr Arg Gln Phe Ala Arg Asp Tyr Glu Leu Thr 865 870 875 880 Gly Phe Met Arg Phe Glu Met Ala Gly Gly Trp Ser Ser Ser Thr Arg 885 890 895 Glu Thr Gly Ser Leu Thr Arg Tyr Phe Ala Arg Gly Ser Gly His Asn 900 905 910 Met Ser Leu Pro Ile Gly Ile Val Ala His Ala Val Ser His Val Arg 915 920 925 Arg Ser Pro Pro Ser Lys Leu Thr Leu Asn Met Gly Tyr Arg Pro Asp 930 935 940 Ile Trp Arg Val Thr Pro His Cys Asn Met Glu Ile Ile Ala Asn Gly 945 950 955 960 Val Lys Thr Pro Ile Gln Gly Ser Pro Leu Ala Arg His Ala Phe Phe 965 970 975 Leu Glu Val His Asp Thr Leu Tyr Ile His His Phe Gly Arg Ala Tyr 980 985 990 Met Asn Tyr Ser Leu Asp Ala Arg Arg Arg Gln Thr Ala His Phe Val 995 1000 1005 Ser Met Gly Leu Asn Arg Ile Phe 1010 1015 42 3051 DNA Chlamydia trachomatis 42 atgccttttt ctttgagatc tacatcattt tgttttttag cttgtttgtg ttcctattcg 60 tatggattcg cgagctctcc tcaagtgtta acacctaatg taaccactcc ttttaagggg 120 gacgatgttt acttgaatgg agactgcgct tttgtcaatg tctatgcagg ggcagagaac 180 ggctcaatta tctcagctaa tggcgacaat ttaacgatta ccggacaaaa ccatacatta 240 tcatttacag attctcaagg gccagttctt caaaattatg ccttcatttc agcaggagag 300 acacttactc tgaaagattt ttcgagtttg atgttctcga aaaatgtttc ttgcggagaa 360 aagggaatga tctcagggaa aaccgtgagt atttccggag caggcgaagt gattttttgg 420 gataactctg tggggtattc tcctttgtct attgtgccag catcgactcc aactcctcca 480 gcaccagcac cagctcctgc tgcttcaagc tctttatctc caacagttag tgatgctcgg 540 aaagggtcta ttttttctgt agagactagt ttggagatct caggcgtcaa aaaaggggtc 600 atgttcgata ataatgccgg gaattttgga acagtttttc gaggtaatag taataataat 660 gctggtagtg ggggtagtgg gtctgctaca acaccaagtt ttacagttaa aaactgtaaa 720 gggaaagttt ctttcacaga taacgtagcc tcctgtggag gcggagtagt ctacaaagga 780 actgtgcttt tcaaagacaa tgaaggaggc atattcttcc gagggaacac agcatacgat 840 gatttaggga ttcttgctgc tactagtcgg gatcagaata cggagacagg aggcggtgga 900 ggagttattt gctctccaga tgattctgta aagtttgaag gcaataaagg ttctattgtt 960 tttgattaca actttgcaaa aggcagaggc ggaagcatcc taacgaaaga attctctctt 1020 gtagcagatg attcggttgt ctttagtaac aatacagcag aaaaaggcgg tggagctatt 1080 tatgctccta ctatcgatat aagcacgaat ggaggatcga ttctatttga aagaaaccga 1140 gctgcagaag gaggcgccat ctgcgtgagt gaagcaagct ctggttcaac tggaaatctt 1200 actttaagcg cttctgatgg ggatattgtt ttttctggga atatgacgag tgatcgtcct 1260 ggagagcgca gcgcagcaag aatcttaagt gatggaacga ctgtttcttt aaatgcttcc 1320 ggactatcga agctgatctt ttatgatcct gtagtacaaa ataattcagc agcgggtgca 1380 tcgacaccat caccatcttc ttcttctatg cctggtgctg tcacgattaa tcagtccggt 1440 aatggatctg tgatttttac cgccgagtca ttgactcctt cagaaaaact tcaagttctt 1500 aactctactt ctaacttccc aggagctctg actgtgtcag gaggggagtt ggttgtgacg 1560 gaaggagcta ccttaactac tgggaccatt acagccacct ctggacgagt gactttagga 1620 tccggagctt cgttgtctgc cgttgcaggt gctgcaaata ataattatac ttgtacagta 1680 tctaagttgg ggattgattt agaatccttt ttaactccta actataagac ggccatactg 1740 ggtgcggatg gaacagttac tgttaacagc ggctctactt tagacctagt gatggagagt 1800 gaggcagagg tatatgataa tccgcttttt gtgggatcgc tgacaattcc ttttgttact 1860 ctatcttcta gtagtgctag taacggagtt acaaaaaatt ctgtcactat taatgatgca 1920 gacgctgcgc actatgggta tcaaggctct tggtctgcag attggacgaa accgcctctg 1980 gctcctgatg ctaaggggat ggtacctcct aataccaata acactctgta tctgacatgg 2040 agacctgctt cgaattacgg tgaatatcga ctggatcctc agagaaaggg agaactagta 2100 cccaactctc tttgggtagc gggatctgca ttaagaacct ttactaatgg tttgaaagaa 2160 cactatgttt ctagagatgt tggatttgta gcatctctgc atgctctcgg ggattatatt 2220 ttgaattata cgcaagatga tcgggatggc tttttagcta gatatggggg attccaggcg 2280 accgcagcct cccattatga aaatgggtca atatttggag tggcttttgg acaactctat 2340 ggtcagacaa agagcagaat gtattactct aaagatgctg ggaacatgac gatgttgtcc 2400 tgtttcggaa gaagttacgt agatattaaa ggaacagaaa ctgttatgta ttgggagacg 2460 gcttatggct attctgtgca cagaatgcat acgcagtatt ttaatgacaa aacgcagaag 2520 ttcgatcatt cgaaatgtca ttggcacaac aataactatt atgcgtttgt gggtgccgag 2580 cataatttct tagagtactg cattcctact cgtcagttcg ctagagatta tgagcttaca 2640 gggtttatgc gttttgaaat ggccggagga tggtccagtt ctacacgaga aactggctcc 2700 ctaactagat atttcgctcg cgggtcaggg cataatatgt cgcttccaat aggaattgta 2760 gctcatgcag tttctcatgt gcgaagatct cctccttcta aactgacact aaatatggga 2820 tatagaccag acatttggcg tgtcactcca cattgcaata tggaaattat tgctaacgga 2880 gtgaagacac ctatacaagg atctccgctg gcacggcatg ccttcttctt agaagtgcat 2940 gatactttgt atattcatca ttttggaaga gcctatatga actattcgct ggatgctcgt 3000 cgtcgacaaa cggcacattt tgtatccatg ggcttgaata gaatctttta a 3051 43 553 PRT Chlamydia trachomatis 43 Met Arg Ile Gly Asp Pro Met Asn Lys Leu Ile Arg Arg Ala Val Thr 1 5 10 15 Ile Phe Ala Val Thr Ser Val Ala Ser Leu Phe Ala Ser Gly Val Leu 20 25 30 Glu Thr Ser Met Ala Glu Ser Leu Ser Thr Asn Val Ile Ser Leu Ala 35 40 45 Asp Thr Lys Ala Lys Asp Asn Thr Ser His Lys Ser Lys Lys Ala Arg 50 55 60 Lys Asn His Ser Lys Glu Thr Pro Val Asp Arg Lys Glu Val Ala Pro 65 70 75 80 Val His Glu Ser Lys Ala Thr Gly Pro Lys Gln Asp Ser Cys Phe Gly 85 90 95 Arg Met Tyr Thr Val Lys Val Asn Asp Asp Arg Asn Val Glu Ile Thr 100 105 110 Gln Ala Val Pro Glu Tyr Ala Thr Val Gly Ser Pro Tyr Pro Ile Glu 115 120 125 Ile Thr Ala Thr Gly Lys Arg Asp Cys Val Asp Val Ile Ile Thr Gln 130 135 140 Gln Leu Pro Cys Glu Ala Glu Phe Val Arg Ser Asp Pro Ala Thr Thr 145 150 155 160 Pro Thr Ala Asp Gly Lys Leu Val Trp Lys Ile Asp Arg Leu Gly Gln 165 170 175 Gly Glu Lys Ser Lys Ile Thr Val Trp Val Lys Pro Leu Lys Glu Gly 180 185 190 Cys Cys Phe Thr Ala Ala Thr Val Cys Ala Cys Pro Glu Ile Arg Ser 195 200 205 Val Thr Lys Cys Gly Gln Pro Ala Ile Cys Val Lys Gln Glu Gly Pro 210 215 220 Glu Asn Ala Cys Leu Arg Cys Pro Val Val Tyr Lys Ile Asn Ile Val 225 230 235 240 Asn Gln Gly Thr Ala Thr Ala Arg Asn Val Val Val Glu Asn Pro Val 245 250 255 Pro Asp Gly Tyr Ala His Ser Ser Gly Gln Arg Val Leu Thr Phe Thr 260 265 270 Leu Gly Asp Met Gln Pro Gly Glu His Arg Thr Ile Thr Val Glu Phe 275 280 285 Cys Pro Leu Lys Arg Gly Arg Ala Thr Asn Ile Ala Thr Val Ser Tyr 290 295 300 Cys Gly Gly His Lys Asn Thr Ala Ser Val Thr Thr Val Ile Asn Glu 305 310 315 320 Pro Cys Val Gln Val Ser Ile Ala Gly Ala Asp Trp Ser Tyr Val Cys 325 330 335 Lys Pro Val Glu Tyr Val Ile Ser Val Ser Asn Pro Gly Asp Leu Val 340 345 350 Leu Arg Asp Val Val Val Glu Asp Thr Leu Ser Pro Gly Val Thr Val 355 360 365 Leu Glu Ala Ala Gly Ala Gln Ile Ser Cys Asn Lys Val Val Trp Thr 370 375 380 Val Lys Glu Leu Asn Pro Gly Glu Ser Leu Gln Tyr Lys Val Leu Val 385 390 395 400 Arg Ala Gln Thr Pro Gly Gln Phe Thr Asn Asn Val Val Val Lys Ser 405 410 415 Cys Ser Asp Cys Gly Thr Cys Thr Ser Cys Ala Glu Ala Thr Thr Tyr 420 425 430 Trp Lys Gly Val Ala Ala Thr His Met Cys Val Val Asp Thr Cys Asp 435 440 445 Pro Val Cys Val Gly Glu Asn Thr Val Tyr Arg Ile Cys Val Thr Asn 450 455 460 Arg Gly Ser Ala Glu Asp Thr Asn Val Ser Leu Met Leu Lys Phe Ser 465 470 475 480 Lys Glu Leu Gln Pro Val Ser Phe Ser Gly Pro Thr Lys Gly Thr Ile 485 490 495 Thr Gly Asn Thr Val Val Phe Asp Ser Leu Pro Arg Leu Gly Ser Lys 500 505 510 Glu Thr Val Glu Phe Ser Val Thr Leu Lys Ala Val Ser Ala Gly Asp 515 520 525 Ala Arg Gly Glu Ala Ile Leu Ser Ser Asp Thr Leu Thr Val Pro Val 530 535 540 Ser Asp Thr Glu Asn Thr His Ile Tyr 545 550 44 1662 DNA Chlamydia trachomatis 44 atgcgaatag gagatcctat gaacaaactc atcagacgag cagtgacgat cttcgcggtg 60 actagtgtgg cgagtttatt tgctagcggg gtgttagaga cctctatggc agagtctctc 120 tctacaaacg ttattagctt agctgacacc aaagcgaaag acaacacttc tcataaaagc 180 aaaaaagcaa gaaaaaacca cagcaaagag actcccgtag accgtaaaga ggttgctccg 240 gttcatgagt ctaaagctac aggacctaaa caggattctt gctttggcag aatgtataca 300 gtcaaagtta atgatgatcg caatgttgaa atcacacaag ctgttcctga atatgctacg 360 gtaggatctc cctatcctat tgaaattact gctacaggta aaagggattg tgttgatgtt 420 atcattactc agcaattacc atgtgaagca gagttcgtac gcagtgatcc agcgacaact 480 cctactgctg atggtaagct agtttggaaa attgaccgct taggacaagg cgaaaagagt 540 aaaattactg tatgggtaaa acctcttaaa gaaggttgct gctttacagc tgcaacagta 600 tgcgcttgtc cagagatccg ttcggttaca aaatgtggac aacctgctat ctgtgttaaa 660 caagaaggcc cagagaatgc ttgtttgcgt tgcccagtag tttacaaaat taatatagtg 720 aaccaaggaa cagcaacagc tcgtaacgtt gttgttgaaa atcctgttcc agatggttac 780 gctcattctt ctggacagcg tgtactgacg tttactcttg gagatatgca acctggagag 840 cacagaacaa ttactgtaga gttttgtccg cttaaacgtg gtcgtgctac caatatagca 900 acggtttctt actgtggagg acataaaaat acagcaagcg taacaactgt gatcaacgag 960 ccttgcgtac aagtaagtat tgcaggagca gattggtctt atgtttgtaa gcctgtagaa 1020 tatgtgatct ccgtttccaa tcctggagat cttgtgttgc gagatgtcgt cgttgaagac 1080 actctttctc ccggagtcac agttcttgaa gctgcaggag ctcaaatttc ttgtaataaa 1140 gtagtttgga ctgtgaaaga actgaatcct ggagagtctc tacagtataa agttctagta 1200 agagcacaaa ctcctggaca attcacaaat aatgttgttg tgaagagctg ctctgactgt 1260 ggtacttgta cttcttgcgc agaagcgaca acttactgga aaggagttgc tgctactcat 1320 atgtgcgtag tagatacttg tgaccctgtt tgtgtaggag aaaatactgt ttaccgtatt 1380 tgtgtcacca acagaggttc tgcagaagat acaaatgttt ctttaatgct taaattctct 1440 aaagaactgc aacctgtatc cttctctgga ccaactaaag gaacgattac aggcaataca 1500 gtagtattcg attcgttacc tagattaggt tctaaagaaa ctgtagagtt ttctgtaaca 1560 ttgaaagcag tatcagctgg agatgctcgt ggggaagcga ttctttcttc cgatacattg 1620 actgttccag tttctgatac agagaataca cacatctatt aa 1662 45 1751 PRT Chlamydia trachomatis 45 Met Lys Trp Leu Ser Ala Thr Ala Val Phe Ala Ala Val Leu Pro Ser 1 5 10 15 Val Ser Gly Phe Cys Phe Pro Glu Pro Lys Glu Leu Asn Phe Ser Arg 20 25 30 Val Gly Thr Ser Ser Ser Thr Thr Phe Thr Glu Thr Val Gly Glu Ala 35 40 45 Gly Ala Glu Tyr Ile Val Ser Gly Asn Ala Ser Phe Thr Lys Phe Thr 50 55 60 Asn Ile Pro Thr Thr Asp Thr Thr Thr Pro Thr Asn Ser Asn Ser Ser 65 70 75 80 Ser Ser Asn Gly Glu Thr Ala Ser Val Ser Glu Asp Ser Asp Ser Thr 85 90 95 Thr Thr Thr Pro Asp Pro Lys Gly Gly Gly Ala Phe Tyr Asn Ala His 100 105 110 Ser Gly Val Leu Ser Phe Met Thr Arg Ser Gly Thr Glu Gly Ser Leu 115 120 125 Thr Leu Ser Glu Ile Lys Ile Thr Gly Glu Gly Gly Ala Ile Phe Ser 130 135 140 Gln Gly Glu Leu Leu Phe Thr Asp Leu Thr Gly Leu Thr Ile Gln Asn 145 150 155 160 Asn Leu Ser Gln Leu Ser Gly Gly Ala Ile Phe Gly Glu Ser Thr Ile 165 170 175 Ser Leu Ser Gly Ile Thr Lys Ala Thr Phe Ser Ser Asn Ser Ala Glu 180 185 190 Val Pro Ala Pro Val Lys Lys Pro Thr Glu Pro Lys Ala Gln Thr Ala 195 200 205 Ser Glu Thr Ser Gly Ser Ser Ser Ser Ser Gly Asn Asp Ser Val Ser 210 215 220 Ser Pro Ser Ser Ser Arg Ala Glu Pro Ala Ala Ala Asn Leu Gln Ser 225 230 235 240 His Phe Ile Cys Ala Thr Ala Thr Pro Ala Ala Gln Thr Asp Thr Glu 245 250 255 Thr Ser Thr Pro Ser His Lys Pro Gly Ser Gly Gly Ala Ile Tyr Ala 260 265 270 Lys Gly Asp Leu Thr Ile Ala Asp Ser Gln Glu Val Leu Phe Ser Ile 275 280 285 Asn Lys Ala Thr Lys Asp Gly Gly Ala Ile Phe Ala Glu Lys Asp Val 290 295 300 Ser Phe Glu Asn Ile Thr Ser Leu Lys Val Gln Thr Asn Gly Ala Glu 305 310 315 320 Glu Lys Gly Gly Ala Ile Tyr Ala Lys Gly Asp Leu Ser Ile Gln Ser 325 330 335 Ser Lys Gln Ser Leu Phe Asn Ser Asn Tyr Ser Lys Gln Gly Gly Gly 340 345 350 Ala Leu Tyr Val Glu Gly Asp Ile Asn Phe Gln Asp Leu Glu Glu Ile 355 360 365 Arg Ile Lys Tyr Asn Lys Ala Gly Thr Phe Glu Thr Lys Lys Ile Thr 370 375 380 Leu Pro Lys Ala Gln Ala Ser Ala Gly Asn Ala Asp Ala Trp Ala Ser 385 390 395 400 Ser Ser Pro Gln Ser Gly Ser Gly Ala Thr Thr Val Ser Asn Ser Gly 405 410 415 Asp Ser Ser Ser Gly Ser Asp Ser Asp Thr Ser Glu Thr Val Pro Ala 420 425 430 Thr Ala Lys Gly Gly Gly Leu Tyr Thr Asp Lys Asn Leu Ser Ile Thr 435 440 445 Asn Ile Thr Gly Ile Ile Glu Ile Ala Asn Asn Lys Ala Thr Asp Val 450 455 460 Gly Gly Gly Ala Tyr Val Lys Gly Thr Leu Thr Cys Glu Asn Ser His 465 470 475 480 Arg Leu Gln Phe Leu Lys Asn Ser Ser Asp Lys Gln Gly Gly Gly Ile 485 490 495 Tyr Gly Glu Asp Asn Ile Thr Leu Ser Asn Leu Thr Gly Lys Thr Leu 500 505 510 Phe Gln Glu Asn Thr Ala Lys Glu Glu Gly Gly Gly Leu Phe Ile Lys 515 520 525 Gly Thr Asp Lys Ala Leu Thr Met Thr Gly Leu Asp Ser Phe Cys Leu 530 535 540 Ile Asn Asn Thr Ser Glu Lys His Gly Gly Gly Ala Phe Val Thr Lys 545 550 555 560 Glu Ile Ser Gln Thr Tyr Thr Ser Asp Val Glu Thr Ile Pro Gly Ile 565 570 575 Thr Pro Val His Gly Glu Thr Val Ile Thr Gly Asn Lys Ser Thr Gly 580 585 590 Gly Asn Gly Gly Gly Val Cys Thr Lys Arg Leu Ala Leu Ser Asn Leu 595 600 605 Gln Ser Ile Ser Ile Ser Gly Asn Ser Ala Ala Glu Asn Gly Gly Gly 610 615 620 Ala His Thr Cys Pro Asp Ser Phe Pro Thr Ala Asp Thr Ala Glu Gln 625 630 635 640 Pro Ala Ala Ala Ser Ala Ala Thr Ser Thr Pro Glu Ser Ala Pro Val 645 650 655 Val Ser Thr Ala Leu Ser Thr Pro Ser Ser Ser Thr Val Ser Ser Leu 660 665 670 Thr Leu Leu Ala Ala Ser Ser Gln Ala Ser Pro Ala Thr Ser Asn Lys 675 680 685 Glu Thr Gln Asp Pro Asn Ala Asp Thr Asp Leu Leu Ile Asp Tyr Val 690 695 700 Val Asp Thr Thr Ile Ser Lys Asn Thr Ala Lys Lys Gly Gly Gly Ile 705 710 715 720 Tyr Ala Lys Lys Ala Lys Met Ser Arg Ile Asp Gln Leu Asn Ile Ser 725 730 735 Glu Asn Ser Ala Thr Glu Ile Gly Gly Gly Ile Cys Cys Lys Glu Ser 740 745 750 Leu Glu Leu Asp Ala Leu Val Ser Leu Ser Val Thr Glu Asn Leu Val 755 760 765 Gly Lys Glu Gly Gly Gly Leu His Ala Lys Thr Val Asn Ile Ser Asn 770 775 780 Leu Lys Ser Gly Phe Ser Phe Ser Asn Asn Lys Ala Asn Ser Ser Ser 785 790 795 800 Thr Gly Val Ala Thr Thr Ala Ser Ala Pro Ala Ala Ala Ala Ala Ser 805 810 815 Leu Gln Ala Ala Ala Ala Ala Val Pro Ser Ser Pro Ala Thr Pro Thr 820 825 830 Tyr Ser Gly Val Val Gly Gly Ala Ile Tyr Gly Glu Lys Val Thr Phe 835 840 845 Ser Gln Cys Ser Gly Thr Cys Gln Phe Ser Gly Asn Gln Ala Ile Asp 850 855 860 Asn Asn Pro Ser Gln Ser Ser Leu Asn Val Gln Gly Gly Ala Ile Tyr 865 870 875 880 Ala Lys Thr Ser Leu Ser Ile Gly Ser Ser Asp Ala Gly Thr Ser Tyr 885 890 895 Ile Phe Ser Gly Asn Ser Val Ser Thr Gly Lys Ser Gln Thr Thr Gly 900 905 910 Gln Ile Ala Gly Gly Ala Ile Tyr Ser Pro Thr Val Thr Leu Asn Cys 915 920 925 Pro Ala Thr Phe Ser Asn Asn Thr Ala Ser Met Ala Thr Pro Lys Thr 930 935 940 Ser Ser Glu Asp Gly Ser Ser Gly Asn Ser Ile Lys Asp Thr Ile Gly 945 950 955 960 Gly Ala Ile Ala Gly Thr Ala Ile Thr Leu Ser Gly Val Ser Arg Phe 965 970 975 Ser Gly Asn Thr Ala Asp Leu Gly Ala Ala Ile Gly Thr Leu Ala Asn 980 985 990 Ala Asn Thr Pro Ser Ala Thr Ser Gly Ser Gln Asn Ser Ile Thr Glu 995 1000 1005 Lys Ile Thr Leu Glu Asn Gly Ser Phe Ile Phe Glu Arg Asn Gln Ala 1010 1015 1020 Asn Lys Arg Gly Ala Ile Tyr Ser Pro Ser Val Ser Ile Lys Gly Asn 1025 1030 1035 1040 Asn Ile Thr Phe Asn Gln Asn Thr Ser Thr His Asp Gly Ser Ala Ile 1045 1050 1055 Tyr Phe Thr Lys Asp Ala Thr Ile Glu Ser Leu Gly Ser Val Leu Phe 1060 1065 1070 Thr Gly Asn Asn Val Thr Ala Thr Gln Ala Ser Ser Ala Thr Ser Gly 1075 1080 1085 Gln Asn Thr Asn Thr Ala Asn Tyr Gly Ala Ala Ile Phe Gly Asp Pro 1090 1095 1100 Gly Thr Thr Gln Ser Ser Gln Thr Asp Ala Ile Leu Thr Leu Leu Ala 1105 1110 1115 1120 Ser Ser Gly Asn Ile Thr Phe Ser Asn Asn Ser Leu Gln Asn Asn Gln 1125 1130 1135 Gly Asp Thr Pro Ala Ser Lys Phe Cys Ser Ile Ala Gly Tyr Val Lys 1140 1145 1150 Leu Ser Leu Gln Ala Ala Lys Gly Lys Thr Ile Ser Phe Phe Asp Cys 1155 1160 1165 Val His Thr Ser Thr Lys Lys Ile Gly Ser Thr Gln Asn Val Tyr Glu 1170 1175 1180 Thr Leu Asp Ile Asn Lys Glu Glu Asn Ser Asn Pro Tyr Thr Gly Thr 1185 1190 1195 1200 Ile Val Phe Ser Ser Glu Leu His Glu Asn Lys Ser Tyr Ile Pro Gln 1205 1210 1215 Asn Ala Ile Leu His Asn Gly Thr Leu Val Leu Lys Glu Lys Thr Glu 1220 1225 1230 Leu His Val Val Ser Phe Glu Gln Lys Glu Gly Ser Lys Leu Ile Met 1235 1240 1245 Lys Pro Gly Ala Val Leu Ser Asn Gln Asn Ile Ala Asn Gly Ala Leu 1250 1255 1260 Val Ile Asn Gly Leu Thr Ile Asp Leu Ser Ser Met Gly Thr Pro Gln 1265 1270 1275 1280 Ala Gly Glu Ile Phe Ser Pro Pro Glu Leu Arg Ile Val Ala Thr Thr 1285 1290 1295 Ser Ser Ala Ser Gly Gly Ser Gly Val Ser Ser Ser Ile Pro Thr Asn 1300 1305 1310 Pro Lys Arg Ile Ser Ala Ala Ala Pro Ser Gly Ser Ala Ala Thr Thr 1315 1320 1325 Pro Thr Met Ser Glu Asn Lys Val Phe Leu Thr Gly Asp Leu Thr Leu 1330 1335 1340 Ile Asp Pro Asn Gly Asn Phe Tyr Gln Asn Pro Met Leu Gly Ser Asp 1345 1350 1355 1360 Leu Asp Val Pro Leu Ile Lys Leu Pro Thr Asn Thr Ser Asp Val Gln 1365 1370 1375 Val Tyr Asp Leu Thr Leu Ser Gly Asp Leu Phe Pro Gln Lys Gly Tyr 1380 1385 1390 Met Gly Thr Trp Thr Leu Asp Ser Asn Pro Gln Thr Gly Lys Leu Gln 1395 1400 1405 Ala Arg Trp Thr Phe Asp Thr Tyr Arg Arg Trp Val Tyr Ile Pro Arg 1410 1415 1420 Asp Asn His Phe Tyr Ala Asn Ser Ile Leu Gly Ser Gln Asn Ser Met 1425 1430 1435 1440 Ile Val Val Lys Gln Gly Leu Ile Asn Asn Met Leu Asn Asn Ala Arg 1445 1450 1455 Phe Asp Asp Ile Ala Tyr Asn Asn Phe Trp Val Ser Gly Val Gly Thr 1460 1465 1470 Phe Leu Ala Gln Gln Gly Thr Pro Leu Ser Glu Glu Phe Ser Tyr Tyr 1475 1480 1485 Ser Arg Gly Thr Ser Val Ala Ile Asp Ala Lys Pro Arg Gln Asp Phe 1490 1495 1500 Ile Leu Gly Ala Ala Phe Ser Lys Met Val Gly Lys Thr Lys Ala Ile 1505 1510 1515 1520 Lys Lys Met His Asn Tyr Phe His Lys Gly Ser Glu Tyr Ser Tyr Gln 1525 1530 1535 Ala Ser Val Tyr Gly Gly Lys Phe Leu Tyr Phe Leu Leu Asn Lys Gln 1540 1545 1550 His Gly Trp Ala Leu Pro Phe Leu Ile Gln Gly Val Val Ser Tyr Gly 1555 1560 1565 His Ile Lys His Asp Thr Thr Thr Leu Tyr Pro Ser Ile His Glu Arg 1570 1575 1580 Asn Lys Gly Asp Trp Glu Asp Leu Gly Trp Leu Ala Asp Leu Arg Ile 1585 1590 1595 1600 Ser Met Asp Leu Lys Glu Pro Ser Lys Asp Ser Ser Lys Arg Ile Thr 1605 1610 1615 Val Tyr Gly Glu Leu Glu Tyr Ser Ser Ile Arg Gln Lys Gln Phe Thr 1620 1625 1630 Glu Ile Asp Tyr Asp Pro Arg His Phe Asp Asp Cys Ala Tyr Arg Asn 1635 1640 1645 Leu Ser Leu Pro Val Gly Cys Ala Val Glu Gly Ala Ile Met Asn Cys 1650 1655 1660 Asn Ile Leu Met Tyr Asn Lys Leu Ala Leu Ala Tyr Met Pro Ser Ile 1665 1670 1675 1680 Tyr Arg Asn Asn Pro Val Cys Lys Tyr Arg Val Leu Ser Ser Asn Glu 1685 1690 1695 Ala Gly Gln Val Ile Cys Gly Val Pro Thr Arg Thr Ser Ala Arg Ala 1700 1705 1710 Glu Tyr Ser Thr Gln Leu Tyr Leu Gly Pro Phe Trp Thr Leu Tyr Gly 1715 1720 1725 Asn Tyr Thr Ile Asp Val Gly Met Tyr Thr Leu Ser Gln Met Thr Ser 1730 1735 1740 Cys Gly Ala Arg Met Ile Phe 1745 1750 46 5256 DNA Chlamydia trachomatis 46 atgaaatggc tgtcagctac tgcggtgttt gctgctgttc tcccctcagt ttcagggttt 60 tgcttcccag aacctaaaga attaaatttc tctcgcgtag gaacttcttc ctctaccact 120 tttactgaaa cagttggaga agctggggca gaatatatcg tctctggtaa cgcatctttc 180 acaaaattta ccaacattcc tactaccgat acaacaactc ccacgaactc aaactcctct 240 agctctaacg gagagactgc ttccgtttct gaggatagtg actctacaac aacgactcct 300 gatcctaaag gtggcggcgc cttttataac gcgcactccg gagttttatc ctttatgaca 360 cgatcaggaa cagaaggttc cttaactctg tctgagataa aaataactgg tgaaggcggt 420 gctatcttct ctcaaggaga gctgctattt acagatctga caggtctaac catccaaaat 480 aacttatccc agctatccgg aggagcgatt tttggagaat ctacaatctc cctatcaggg 540 attactaaag cgactttctc ctccaactct gcagaagttc ctgctcctgt taagaaacct 600 acagaaccta aagctcaaac agcaagcgaa acgtcgggtt ctagtagttc tagcggaaat 660 gattcggtgt cttcccccag ttccagtaga gctgaacccg cagcagctaa tcttcaaagt 720 cactttattt gtgctacagc tactcctgct gctcaaaccg atacagaaac atcaactccc 780 tctcataagc caggatctgg gggagctatc tatgctaaag gcgaccttac tatcgcagac 840 tctcaagagg tactattctc aataaataaa gctactaaag atggaggagc gatctttgct 900 gagaaagatg tttctttcga gaatattaca tcattaaaag tacaaactaa cggtgctgaa 960 gaaaagggag gagctatcta tgctaaaggt gacctctcaa ttcaatcttc taaacagagt 1020 ctttttaatt ctaactacag taaacaaggt ggtggggctc tatatgttga aggagatata 1080 aacttccaag atcttgaaga aattcgcatt aagtacaata aagctggaac gttcgaaaca 1140 aaaaaaatca ctttaccaaa agctcaagca tctgcaggaa atgcagatgc ttgggcctct 1200 tcctctcctc aatctggttc tggagcaact acagtctcca actcaggaga ctctagctct 1260 ggctcagact cggatacctc agaaacagtt ccagccacag ctaaaggcgg tgggctttat 1320 actgataaga atctttcgat tactaacatc acaggaatta tcgaaattgc aaataacaaa 1380 gcgacagatg ttggaggtgg tgcttacgta aaaggaaccc ttacttgtga aaactctcac 1440 cgtctacaat ttttgaaaaa ctcttccgat aaacaaggtg gaggaatcta cggagaagac 1500 aacatcaccc tatctaattt gacagggaag actctattcc aagagaatac tgccaaagaa 1560 gagggcggtg gactcttcat aaaaggtaca gataaagctc ttacaatgac aggactggat 1620 agtttctgtt taattaataa cacatcagaa aaacatggtg gtggagcctt tgttaccaaa 1680 gaaatctctc agacttacac ctctgatgtg gaaacaattc caggaatcac gcctgtacat 1740 ggtgaaacag tcattactgg caataaatct acaggaggta atggtggagg cgtgtgtaca 1800 aaacgtcttg ccttatctaa ccttcaaagc atttctatat ccgggaattc tgcagctgaa 1860 aatggtggtg gagcccacac atgcccagat agcttcccaa cggcggatac tgcagaacag 1920 cccgcagcag cttctgccgc gacgtctact cccgagtctg ccccagtggt ctcaactgct 1980 ctaagcacac cttcatcttc taccgtctct tcattaacct tactagcagc ctcttcacaa 2040 gcctctcctg caacctctaa taaggaaact caagatccta atgctgatac agacttattg 2100 atcgattatg tagttgatac gactatcagc aaaaacactg ctaagaaagg cggtggaatc 2160 tatgctaaaa aagccaagat gtcccgcata gaccaactga atatctctga gaactccgct 2220 acagagatag gtggaggtat ctgctgtaaa gaatctttag aactagatgc cctagtctcc 2280 ttatctgtaa cagagaacct tgttgggaaa gaaggtggag gcttacatgc taaaactgta 2340 aatatttcta atctgaaatc aggcttctct ttctcgaaca acaaagcaaa ctcctcatcc 2400 acaggagtcg caacaacagc ttcagcacct gctgcagctg ctgcttccct acaagcagcc 2460 gcagcagccg taccatcatc tccagcaaca ccaacttatt caggtgtagt aggaggagct 2520 atctatggag aaaaggttac attctctcaa tgtagcggga cttgtcagtt ctctgggaac 2580 caagctatcg ataacaatcc ctcccaatca tcgttgaacg tacaaggagg agccatctat 2640 gccaaaacct ctttgtctat tggatcttcc gatgctggaa cctcctatat tttctcgggg 2700 aacagtgtct ccactgggaa atctcaaaca acagggcaaa tagcgggagg agcgatctac 2760 tcccctactg ttacattgaa ttgtcctgcg acattctcta acaatacagc ctctatggct 2820 acaccaaaga cttcttctga agatggatcc tcaggaaatt ctattaaaga taccattgga 2880 ggagccattg cagggacagc cattacccta tctggagtct ctcgattttc agggaatacg 2940 gctgatttag gagctgcaat aggaactcta gctaatgcaa atacacccag tgcaactagc 3000 ggatctcaaa atagcattac agaaaaaatt actttagaaa acggttcttt tatttttgaa 3060 agaaaccaag ctaataaacg tggagcgatt tactctccta gcgtttccat taaagggaat 3120 aatattacct tcaatcaaaa tacatccact catgatggaa gtgctatcta ctttacaaaa 3180 gatgctacga ttgagtcttt aggatctgtt ctttttacag gaaataacgt tacagctaca 3240 caagctagtt ctgcaacatc tggacaaaat acaaatactg ccaactatgg ggcagccatc 3300 tttggagatc caggaaccac tcaatcgtct caaacagatg ccattttaac ccttcttgct 3360 tcttctggaa acattacttt tagcaacaac agtttacaga ataaccaagg tgatactccc 3420 gctagcaagt tttgtagtat tgcaggatac gtcaaactct ctctacaagc cgctaaaggg 3480 aagactatta gctttttcga ttgtgtgcac acctctacca aaaaaatagg ttcaacacaa 3540 aacgtttatg aaactttaga tattaataaa gaagagaaca gtaatccata tacaggaact 3600 attgtgttct cttctgaatt acatgaaaac aaatcttaca tcccacagaa tgcaatcctt 3660 cacaacggaa ctttagttct taaagagaaa acagaactcc acgtagtctc ttttgagcag 3720 aaagaagggt ctaaattaat tatgaaaccc ggagctgtgt tatctaacca aaacatagct 3780 aacggagctc tagttatcaa tgggttaacg attgatcttt ccagtatggg gactcctcaa 3840 gcaggggaaa tcttctctcc tccagaatta cgtatcgttg ccacgacctc tagtgcatcc 3900 ggaggaagcg gggtcagcag tagtatacca acaaatccta aaaggatttc tgcagcagcg 3960 ccttcaggtt ctgccgcaac tactccaact atgagcgaga acaaagtttt cctaacagga 4020 gaccttactt taatagatcc taatggaaac ttttaccaaa accctatgtt aggaagcgat 4080 ctagatgtac cactaattaa gcttccgact aacacaagtg acgtccaagt ctatgattta 4140 actttatctg gggatctttt ccctcagaaa gggtacatgg gaacctggac attagattct 4200 aatccacaaa cagggaaact tcaagccaga tggacattcg atacctatcg tcgctgggta 4260 tacataccta gggataatca tttttatgcg aactctatct taggctccca aaactcaatg 4320 attgttgtga agcaagggct tatcaacaac atgttgaata atgcccgctt cgatgatatc 4380 gcttacaata acttctgggt ttcaggagta ggaactttct tagctcaaca aggaactcct 4440 ctttccgaag aattcagtta ctacagccgc ggaacttcag ttgccatcga tgccaaacct 4500 agacaagatt ttatcctagg agctgcattt agtaagatgg tggggaaaac caaagccatc 4560 aaaaaaatgc ataattactt ccataagggc tctgagtact cttaccaagc ttctgtctat 4620 ggaggtaaat tcctgtattt cttgctcaat aagcaacatg gttgggcact tcctttccta 4680 atacaaggag tcgtgtccta tggacatatt aaacatgata caacaacact ttacccttct 4740 atccatgaaa gaaataaagg agattgggaa gatttaggat ggttagcgga tcttcgtatc 4800 tctatggatc ttaaagaacc ttctaaagat tcttctaaac ggatcactgt ctatggggaa 4860 cttgagtatt ccagcattcg ccagaaacag ttcacagaaa tcgattacga tccaagacac 4920 ttcgatgatt gtgcttacag aaatctgtcg cttcctgtgg gatgcgctgt cgaaggagct 4980 atcatgaact gtaatattct tatgtataat aagcttgcat tagcctacat gccttctatc 5040 tacagaaata atcctgtctg taaatatcgg gtattgtctt cgaatgaagc tggtcaagtt 5100 atctgcggag tgccaactag aacctctgct agagcagaat acagtactca actatatctt 5160 ggtcccttct ggactctcta cggaaactat actatcgatg taggcatgta tacgctatcg 5220 caaatgacta gctgcggtgc tcgcatgatc ttctaa 5256 47 975 PRT Chlamydia trachomatis 47 Met Asn Arg Val Ile Glu Ile His Ala His Tyr Asp Gln Arg Gln Leu 1 5 10 15 Ser Gln Ser Pro Asn Thr Asn Phe Leu Val His His Pro Tyr Leu Thr 20 25 30 Leu Ile Pro Lys Phe Leu Leu Gly Ala Leu Ile Val Tyr Ala Pro Tyr 35 40 45 Ser Phe Ala Glu Met Glu Leu Ala Ile Ser Gly His Lys Gln Gly Lys 50 55 60 Asp Arg Asp Thr Phe Thr Met Ile Ser Ser Cys Pro Glu Gly Thr Asn 65 70 75 80 Tyr Ile Ile Asn Arg Lys Leu Ile Leu Ser Asp Phe Ser Leu Leu Asn 85 90 95 Lys Val Ser Ser Gly Gly Ala Phe Arg Asn Leu Ala Gly Lys Ile Ser 100 105 110 Phe Leu Gly Lys Asn Ser Ser Ala Ser Ile His Phe Lys His Ile Asn 115 120 125 Ile Asn Gly Phe Gly Ala Gly Val Phe Ser Glu Ser Ser Ile Glu Phe 130 135 140 Thr Asp Leu Arg Lys Leu Val Ala Phe Gly Ser Glu Ser Thr Gly Gly 145 150 155 160 Ile Phe Thr Ala Lys Glu Asp Ile Ser Phe Lys Asn Asn His His Ile 165 170 175 Ala Phe Arg Asn Asn Ile Thr Lys Gly Asn Gly Gly Val Ile Gln Leu 180 185 190 Gln Gly Asp Met Lys Gly Ser Val Ser Phe Val Asp Gln Arg Gly Ala 195 200 205 Ile Ile Phe Thr Asn Asn Gln Ala Val Thr Ser Ser Ser Met Lys His 210 215 220 Ser Gly Arg Gly Gly Ala Ile Ser Gly Asp Phe Ala Gly Ser Arg Ile 225 230 235 240 Leu Phe Leu Asn Asn Gln Gln Ile Thr Phe Glu Gly Asn Ser Ala Val 245 250 255 His Gly Gly Ala Ile Tyr Asn Lys Asn Gly Leu Val Glu Phe Leu Gly 260 265 270 Asn Ala Gly Pro Leu Ala Phe Lys Glu Asn Thr Thr Ile Ala Asn Gly 275 280 285 Gly Ala Ile Tyr Thr Ser Asn Phe Lys Ala Asn Gln Gln Thr Ser Pro 290 295 300 Ile Leu Phe Ser Gln Asn His Ala Asn Lys Lys Gly Gly Ala Ile Tyr 305 310 315 320 Ala Gln Tyr Val Asn Leu Glu Gln Asn Gln Asp Thr Ile Arg Phe Glu 325 330 335 Lys Asn Thr Ala Lys Glu Gly Gly Gly Ala Ile Thr Ser Ser Gln Cys 340 345 350 Ser Ile Thr Ala His Asn Thr Ile Ile Phe Ser Asp Asn Ala Ala Gly 355 360 365 Asp Leu Gly Gly Gly Ala Ile Leu Leu Glu Gly Lys Lys Pro Ser Leu 370 375 380 Thr Leu Ile Ala His Ser Gly Asn Ile Ala Phe Ser Gly Asn Thr Met 385 390 395 400 Leu His Ile Thr Lys Lys Ala Ser Leu Asp Arg His Asn Ser Ile Leu 405 410 415 Ile Lys Glu Ala Pro Tyr Lys Ile Gln Leu Ala Ala Asn Lys Asn His 420 425 430 Ser Ile His Phe Phe Asp Pro Val Met Ala Leu Ser Ala Ser Ser Ser 435 440 445 Pro Ile Gln Ile Asn Ala Pro Glu Tyr Glu Thr Pro Phe Phe Ser Pro 450 455 460 Lys Gly Met Ile Val Phe Ser Gly Ala Asn Leu Leu Asp Asp Ala Arg 465 470 475 480 Glu Asp Val Ala Asn Arg Thr Ser Ile Phe Asn Gln Pro Val His Leu 485 490 495 Tyr Asn Gly Thr Leu Ser Ile Glu Asn Gly Ala His Leu Ile Val Gln 500 505 510 Ser Phe Lys Gln Thr Gly Gly Arg Ile Ser Leu Ser Pro Gly Ser Ser 515 520 525 Leu Ala Leu Tyr Thr Met Asn Ser Phe Phe His Gly Asn Ile Ser Ser 530 535 540 Lys Glu Pro Leu Glu Ile Asn Gly Leu Ser Phe Gly Val Asp Ile Ser 545 550 555 560 Pro Ser Asn Leu Gln Ala Glu Ile Arg Ala Gly Asn Ala Pro Leu Arg 565 570 575 Leu Ser Gly Ser Pro Ser Ile His Asp Pro Glu Gly Leu Phe Tyr Glu 580 585 590 Asn Arg Asp Thr Ala Ala Ser Pro Tyr Gln Met Glu Ile Leu Leu Thr 595 600 605 Ser Asp Lys Ile Val Asp Ile Ser Lys Phe Thr Thr Asp Ser Leu Val 610 615 620 Thr Asn Lys Gln Ser Gly Phe Gln Gly Ala Trp His Phe Ser Trp Gln 625 630 635 640 Pro Asn Thr Ile Asn Asn Thr Lys Gln Lys Ile Leu Arg Ala Ser Trp 645 650 655 Leu Pro Thr Gly Glu Tyr Val Leu Glu Ser Asn Arg Val Gly Arg Ala 660 665 670 Val Pro Asn Ser Leu Trp Ser Thr Phe Leu Leu Leu Gln Thr Ala Ser 675 680 685 His Asn Leu Gly Asp His Leu Cys Asn Asn Arg Ser Leu Ile Pro Thr 690 695 700 Ser Tyr Phe Gly Val Leu Ile Gly Gly Thr Gly Ala Glu Met Ser Thr 705 710 715 720 His Ser Ser Glu Glu Glu Ser Phe Ile Ser Arg Leu Gly Ala Thr Gly 725 730 735 Thr Ser Ile Ile Arg Leu Thr Pro Ser Leu Thr Leu Ser Gly Gly Gly 740 745 750 Ser His Met Phe Gly Asp Ser Phe Val Ala Asp Leu Pro Glu His Ile 755 760 765 Thr Ser Glu Gly Ile Val Gln Asn Val Gly Leu Thr His Val Trp Gly 770 775 780 Pro Leu Thr Val Asn Ser Thr Leu Cys Ala Ala Leu Asp His Asn Ala 785 790 795 800 Met Val Arg Ile Cys Ser Lys Lys Asp His Thr Tyr Gly Lys Trp Asp 805 810 815 Thr Phe Gly Met Arg Gly Thr Leu Gly Ala Ser Tyr Thr Phe Leu Glu 820 825 830 Tyr Asp Gln Thr Met Arg Val Phe Ser Phe Ala Asn Ile Glu Ala Thr 835 840 845 Asn Ile Leu Gln Arg Ala Phe Thr Glu Thr Gly Tyr Asn Pro Arg Ser 850 855 860 Phe Ser Lys Thr Lys Leu Leu Asn Ile Ala Ile Pro Ile Gly Ile Gly 865 870 875 880 Tyr Glu Phe Cys Leu Gly Asn Ser Ser Phe Ala Leu Leu Gly Lys Gly 885 890 895 Ser Ile Gly Tyr Ser Arg Asp Ile Lys Arg Glu Asn Pro Ser Thr Leu 900 905 910 Ala His Leu Ala Met Asn Asp Phe Ala Trp Thr Thr Asn Gly Cys Ser 915 920 925 Val Pro Thr Ser Ala His Thr Leu Ala Asn Gln Leu Ile Leu Arg Tyr 930 935 940 Lys Ala Cys Ser Leu Tyr Ile Thr Ala Tyr Thr Ile Asn Arg Glu Gly 945 950 955 960 Lys Asn Leu Ser Asn Ser Leu Ser Cys Gly Gly Tyr Val Gly Phe 965 970 975 48 2928 DNA Chlamydia trachomatis 48 atgaatcgag ttatagaaat ccatgctcac tacgatcaaa gacaactttc tcaatctcca 60 aatacaaact tcttagtaca tcatccttat cttactctta ttcccaagtt tctactagga 120 gctctaatcg tctatgctcc ttattcgttt gcagaaatgg aattagctat ttctggacat 180 aaacaaggta aagatcgaga tacctttacc atgatctctt cctgtcctga aggcactaat 240 tacatcatca atcgcaaact catactcagt gatttctcgt tactaaataa agtttcatca 300 gggggagcct ttcggaatct agcagggaaa atttccttct taggaaaaaa ttcttctgcg 360 tccattcatt ttaaacacat taatatcaat ggttttggag ccggagtctt ttctgaatcc 420 tctattgaat ttactgattt acgaaaactt gttgcttttg gatctgaaag cacaggagga 480 atttttactg cgaaagagga catctctttt aaaaacaacc accacattgc cttccgcaat 540 aatatcacca aagggaatgg tggcgttatc cagctccaag gagatatgaa aggaagcgta 600 tcctttgtag atcaacgtgg agctatcatc tttaccaata accaagctgt aacttcttca 660 tcaatgaaac atagtggtcg tggaggagca attagcggtg acttcgcagg atccagaatt 720 ctttttctta ataaccaaca aattactttc gaaggcaata gcgctgtgca tggaggtgct 780 atctacaata agaatggcct tgtcgagttc ttaggaaatg caggacctct tgcctttaaa 840 gagaacacaa caatagctaa cgggggagct atatacacaa gtaatttcaa agcgaatcaa 900 caaacatccc ccattctatt ctctcaaaat catgcgaata agaaaggcgg agcgatttac 960 gcgcaatatg tgaacttaga acagaatcaa gatactattc gctttgaaaa aaataccgct 1020 aaagaaggcg gtggagccat cacctcttct caatgctcaa ttactgctca taataccatc 1080 attttttccg ataatgctgc cggagatctt ggaggaggag caattcttct agaagggaaa 1140 aaaccttctc taaccttgat tgctcatagt ggtaatattg catttagcgg caataccatg 1200 cttcatatca ccaaaaaagc ttccctagat cgacacaatt ctatcttaat caaagaagct 1260 ccctataaaa tccaacttgc agcgaacaaa aaccattcta ttcatttctt tgatcctgtc 1320 atggcattgt cagcatcatc ttcccctata caaatcaatg ctcctgagta tgaaactccc 1380 ttcttctcac ctaagggtat gatcgttttc tcgggtgcga atcttttaga tgatgctagg 1440 gaagatgttg caaatagaac atcgattttt aaccaacccg ttcatctata taatggcacc 1500 ctatctatcg aaaatggagc ccatctgatt gtccaaagct tcaaacagac cggaggacgt 1560 atcagtttat ctccaggatc ctccttggct ctatacacga tgaactcgtt cttccatggc 1620 aacatatcca gcaaagaacc cctagaaatt aatggtttaa gctttggagt agatatctct 1680 ccttctaatc ttcaagcaga gatccgtgcc ggcaacgctc ctttacgatt atccggatcc 1740 ccatctatcc atgatcctga aggattattc tacgaaaatc gcgatactgc agcatcacca 1800 taccaaatgg aaatcttgct cacctctgat aaaattgtag atatctccaa atttactact 1860 gattctctag ttacgaacaa acaatcagga ttccaaggag cctggcattt tagctggcag 1920 ccaaatacta taaacaatac taaacaaaaa atattaagag cttcttggct cccaacagga 1980 gaatatgtcc ttgaatccaa tcgagtgggg cgtgccgttc ctaattcctt atggagcaca 2040 tttttacttt tacagacagc ctctcataac ttaggcgatc atctatgtaa taatcgatct 2100 cttattccta cttcatactt cggagtttta attggaggaa ctggagcaga aatgtctacc 2160 cactcctcag aagaagaaag ctttatatct cgtttaggag ctacaggaac ctctatcata 2220 cgcttaactc cctccctgac actctctgga ggaggctcac atatgttcgg agattcgttc 2280 gttgcagact taccagaaca catcacttca gaaggaattg ttcagaatgt cggtttaacc 2340 catgtctggg gaccccttac tgtcaattct acattatgtg cagccttaga tcacaacgcg 2400 atggtccgca tatgctccaa aaaagatcac acctatggga aatgggatac attcggtatg 2460 cgaggaacat taggagcctc ttatacattc ctagaatatg atcaaactat gcgcgtattc 2520 tcattcgcca acatcgaagc cacaaatatc ttgcaaagag cttttactga aacaggctat 2580 aacccaagaa gtttttccaa gacaaaactt ctaaacatcg ccatccccat agggattggt 2640 tatgaattct gcttagggaa tagctctttt gctctactag gtaagggatc catcggttac 2700 tctcgagata ttaaacgaga aaacccatcc actcttgctc acctggctat gaatgatttt 2760 gcttggacta ccaatggctg ttcagttcca acctctgcac acacattggc aaatcaattg 2820 attcttcgct ataaagcatg ttccttatac atcacggcat atactatcaa ccgtgaaggg 2880 aagaacctct ccaatagctt atcctgcgga ggctatgttg gcttctaa 2928 49 210 PRT Chlamydia trachomatis 49 Met Tyr Lys Tyr Phe Ile Val Asp Thr Ser Gly Ser Gln Pro Phe Leu 1 5 10 15 Ala Tyr Val Asp Cys Arg Asp Val Leu Glu Val Trp Ser Leu Pro Thr 20 25 30 Gly Pro Asp Gln Gly Val Val Leu Asn Phe Ile Phe Asn Ser Leu Asp 35 40 45 Leu Pro Phe Gln Gly Ile Gly Val Ser Val Gly Pro Gly Gly Phe Ser 50 55 60 Ala Thr Arg Val Gly Val Ala Phe Ala Gln Gly Leu Ser Leu Ala Lys 65 70 75 80 Asn Val Pro Leu Val Gly Tyr Ser Ser Leu Glu Gly Tyr Leu Ser Leu 85 90 95 Gly Gln Glu Glu Glu Ala Leu Leu Leu Pro Leu Gly Lys Lys Gly Gly 100 105 110 Ile Val Ala Leu Asn Ser Glu Leu Ser Leu Asp Gly Phe Leu Leu Thr 115 120 125 Asp Thr Thr Ser Thr Pro Gly Ile Leu Leu Ser Tyr Ser Glu Ala Leu 130 135 140 Glu Tyr Cys Leu Asp Lys Gly Cys Cys His Val Ile Ser Pro Asp Pro 145 150 155 160 Thr Tyr Phe Val Glu Leu Phe Ser Ser Arg Ile Ser Val Arg Lys Val 165 170 175 Val Pro Cys Ile Asp Arg Ile Arg Lys Tyr Val Val Ser Gln Phe Val 180 185 190 Leu Ser Gln Asn Leu Pro Leu Cys Leu Asp Tyr Arg Ser Ile Ser Ser 195 200 205 Phe Phe 210 50 633 DNA Chlamydia trachomatis 50 gtgtacaaat attttattgt agacacttcc ggttctcagc cgtttttggc ctatgtcgat 60 tgtcgagatg ttttagaagt atggtctttg cctacagggc cagatcaagg agttgtgtta 120 aatttcattt tcaacagcct ggatttgcct ttccaaggta ttggagtctc tgttggtcct 180 gggggatttt ctgcaactag agtgggagtt gcttttgctc aagggctctc tctggctaaa 240 aatgtccctt tagttggcta tagctcttta gaaggatatc tttctttggg tcaagaagag 300 gaggctttgc ttttgcctct agggaaaaag ggtgggattg tagctttaaa ctcagagctt 360 tctcttgatg gttttctgct tacagatact acttctactc cgggaatttt attgtcttat 420 tctgaagctc tagagtattg tttagataag ggatgttgtc atgtgatctc tccggatcca 480 acgtatttcg tagaactatt ttcttcgcgt atttcggtaa ggaaggtggt tccttgtatc 540 gatcgaatcc gtaagtacgt tgtttcgcag tttgttctgt ctcaaaatct tccgttgtgt 600 ttagattacc ggagcatctc ttcctttttt tag 633 51 141 PRT Chlamydia trachomatis 51 Met Ile Asp Val Ser Val Val Gly Pro Val Leu Ala Met Ala Leu Ala 1 5 10 15 Met Ile Gly Ser Ala Val Gly Cys Gly Met Ala Gly Val Ala Ser His 20 25 30 Ala Val Met Ser Arg Ile Asp Glu Gly His Gly Lys Ile Ile Gly Leu 35 40 45 Ser Ala Met Pro Ser Ser Gln Ser Ile Tyr Gly Leu Ile Phe Met Leu 50 55 60 Leu Leu Asn Asp Ala Ile Lys Asp Gly Lys Val Ser Ala Val Ser Gly 65 70 75 80 Ile Val Met Gly Ile Ala Val Gly Ser Ala Leu Leu Leu Ser Ala Phe 85 90 95 Met Gln Gly Lys Cys Cys Val Ser Ala Ile Gln Ala Tyr Ala Arg Ser 100 105 110 Ser Ala Ile Tyr Gly Lys Ser Phe Ala Ser Ile Gly Ile Val Glu Ser 115 120 125 Phe Ala Leu Phe Ala Phe Val Phe Ala Leu Leu Leu Phe 130 135 140 52 426 DNA Chlamydia trachomatis 52 atgatagatg tatcagtagt ggggcctgta ttggctatgg ctttggcaat gattggtagc 60 gctgttggat gtggaatggc tggagtcgct tctcacgcag tgatgtctcg aatcgatgaa 120 ggacacggga agattattgg tctgtctgct atgccctcat cacaatccat ttacgggttg 180 attttcatgt tactgctgaa tgatgcaatt aaggatggaa aagtctctgc agtcagtggt 240 atcgtaatgg gtatagctgt aggatctgcg ttattgcttt ctgcttttat gcaagggaag 300 tgctgtgtga gtgctattca agcctatgcg cgttcctctg caatatatgg taaatcattt 360 gcttcgattg ggattgttga gtcttttgcg ttatttgctt tcgtttttgc gctattgtta 420 ttctaa 426 53 101 PRT Chlamydia trachomatis 53 Met Ala Lys Lys Ser Ala Val Ala Arg Glu Val Lys Arg Arg Lys Leu 1 5 10 15 Val Glu Ala Asn Phe Gln Lys Arg Ala Glu Leu Arg Lys Leu Ala Lys 20 25 30 Ser Leu Ser Val Ser Glu Glu Glu Arg Glu Arg Ala Arg Glu Ala Leu 35 40 45 Asn Lys Met Arg Arg Asp Thr Ser Pro Ser Arg Leu His Asn Arg Cys 50 55 60 Leu Leu Thr Gly Arg Pro Arg Gly Tyr Leu Arg Lys Phe Ala Ile Ser 65 70 75 80 Arg Ile Cys Phe Arg Gln Met Ala Ser Met Gly Asp Ile Pro Gly Val 85 90 95 Val Lys Ala Ser Trp 100 54 306 DNA Chlamydia trachomatis 54 atggcgaaga agtcagcagt agctagagaa gttaaacgtc gaaagttagt agaagctaat 60 tttcagaaga gagcagagct tcgaaaactt gcgaagagtt tatctgttag cgaggaagaa 120 agagaaagag ctcgggaagc tctcaataaa atgagaagag atacttctcc ttctcgttta 180 cataatagat gcctattaac aggccgtcct cgtggatacc ttagaaagtt tgctatctca 240 agaatttgtt ttagacaaat ggcttctatg ggagatatcc caggcgttgt gaaagcaagt 300 tggtag 306 55 393 PRT Chlamydia trachomatis 55 Met Lys Lys Leu Leu Lys Ser Val Leu Val Phe Ala Ala Leu Ser Ser 1 5 10 15 Ala Ser Ser Leu Gln Ala Leu Pro Val Gly Asn Pro Ala Glu Pro Ser 20 25 30 Leu Met Ile Asp Gly Ile Leu Trp Glu Gly Phe Gly Gly Asp Pro Cys 35 40 45 Asp Pro Cys Ala Thr Trp Cys Asp Ala Ile Ser Met Arg Val Gly Tyr 50 55 60 Tyr Gly Asp Phe Val Phe Asp Arg Val Leu Lys Thr Asp Val Asn Lys 65 70 75 80 Glu Phe Gln Met Gly Ala Lys Pro Thr Thr Asp Thr Gly Asn Ser Ala 85 90 95 Ala Pro Ser Thr Leu Thr Ala Arg Glu Asn Pro Ala Tyr Gly Arg His 100 105 110 Met Gln Asp Ala Glu Met Phe Thr Asn Ala Ala Cys Met Ala Leu Asn 115 120 125 Ile Trp Asp Arg Phe Asp Val Phe Cys Thr Leu Gly Ala Thr Ser Gly 130 135 140 Tyr Leu Lys Gly Asn Ser Ala Ser Phe Asn Leu Val Gly Leu Phe Gly 145 150 155 160 Asp Asn Glu Asn Gln Lys Thr Val Lys Ala Glu Ser Val Pro Asn Met 165 170 175 Ser Phe Asp Gln Ser Val Val Glu Leu Tyr Thr Asp Thr Thr Phe Ala 180 185 190 Trp Ser Val Gly Ala Arg Ala Ala Leu Trp Glu Cys Gly Cys Ala Thr 195 200 205 Leu Gly Ala Ser Phe Gln Tyr Ala Gln Ser Lys Pro Lys Val Glu Glu 210 215 220 Leu Asn Val Leu Cys Asn Ala Ala Glu Phe Thr Ile Asn Lys Pro Lys 225 230 235 240 Gly Tyr Val Gly Lys Glu Phe Pro Leu Asp Leu Thr Ala Gly Thr Asp 245 250 255 Ala Ala Thr Gly Thr Lys Asp Ala Ser Ile Asp Tyr His Glu Trp Gln 260 265 270 Ala Ser Leu Ala Leu Ser Tyr Arg Leu Asn Met Phe Thr Pro Tyr Ile 275 280 285 Gly Val Lys Trp Ser Arg Ala Ser Phe Asp Ala Asp Thr Ile Arg Ile 290 295 300 Ala Gln Pro Lys Ser Ala Thr Ala Ile Phe Asp Thr Thr Thr Leu Asn 305 310 315 320 Pro Thr Ile Ala Gly Ala Gly Asp Val Lys Thr Gly Ala Glu Gly Gln 325 330 335 Leu Gly Asp Thr Met Gln Ile Val Ser Leu Gln Leu Asn Lys Met Lys 340 345 350 Ser Arg Lys Ser Cys Gly Ile Ala Val Gly Thr Thr Ile Val Asp Ala 355 360 365 Asp Lys Tyr Ala Val Thr Val Glu Thr Arg Leu Ile Asp Glu Arg Ala 370 375 380 Ala His Val Asn Ala Gln Phe Arg Phe 385 390 56 1182 DNA Chlamydia trachomatis 56 atgaaaaaac tcttgaaatc ggtattagta tttgccgctt tgagttctgc ttcctccttg 60 caagctctgc ctgtggggaa tcctgctgaa ccaagcctta tgatcgacgg aattctgtgg 120 gaaggtttcg gcggagatcc ttgcgatcct tgcgccactt ggtgtgacgc tatcagcatg 180 cgtgttggtt actacggaga ctttgttttc gaccgtgttt tgaaaactga tgtgaataaa 240 gaatttcaga tgggtgccaa gcctacaact gatacaggca atagtgcagc tccatccact 300 cttacagcaa gagagaatcc tgcttacggc cgacatatgc aggatgctga gatgtttaca 360 aatgccgctt gcatggcatt gaatatttgg gatcgttttg atgtattctg tacattagga 420 gccaccagtg gatatcttaa aggaaactct gcttctttca atttagttgg attgtttgga 480 gataatgaaa atcaaaaaac ggtcaaagcg gagtctgtac caaatatgag ctttgatcaa 540 tctgttgttg agttgtatac agatactact tttgcgtgga gcgtcggcgc tcgcgcagct 600 ttgtgggaat gtggatgtgc aactttagga gcttcattcc aatatgctca atctaaacct 660 aaagtagaag aattaaacgt tctctgcaat gcagcagagt ttactattaa taaacctaaa 720 gggtatgtag gtaaggagtt tcctcttgat cttacagcag gaacagatgc tgcgacagga 780 actaaggatg cctctattga ttaccatgaa tggcaagcaa gtttagctct ctcttacaga 840 ctgaatatgt tcactcccta cattggagtt aaatggtctc gagcaagctt tgatgccgat 900 acgattcgta tagcccagcc aaaatcagct acagctattt ttgatactac cacgcttaac 960 ccaactattg ctggagctgg cgatgtgaaa actggcgcag agggtcagct cggagacaca 1020 atgcaaatcg tttccttgca attgaacaag atgaaatcta gaaaatcttg cggtattgca 1080 gtaggaacaa ctattgtgga tgcagacaaa tacgcagtta cagttgagac tcgcttgatc 1140 gatgagagag cagctcacgt aaatgcacaa ttccgcttct aa 1182 57 194 PRT Chlamydia trachomatis 57 Met Leu Lys Met Phe Trp Leu Asn Ser Leu Val Phe Phe Ser Leu Leu 1 5 10 15 Leu Ser Ala Cys Gly Tyr Thr Val Leu Ser Pro His Tyr Val Glu Lys 20 25 30 Lys Phe Ser Leu Ser Glu Gly Ile Tyr Val Cys Pro Ile Glu Gly Asp 35 40 45 Ser Leu Gly Asp Leu Val Ser Ser Leu Ser Tyr Glu Leu Glu Lys Arg 50 55 60 Gly Leu His Thr Arg Ser Gln Gly Thr Ser Ser Gly Tyr Val Leu Lys 65 70 75 80 Val Ser Leu Phe Asn Glu Thr Asp Glu Asn Ile Gly Phe Ala Tyr Thr 85 90 95 Pro Gln Lys Pro Asp Glu Lys Pro Val Lys His Phe Ile Val Ser Asn 100 105 110 Glu Gly Arg Leu Ala Leu Ser Ala Lys Val Gln Leu Ile Lys Asn Arg 115 120 125 Thr Gln Glu Ile Leu Val Glu Lys Cys Leu Arg Lys Ser Val Thr Phe 130 135 140 Asp Phe Gln Pro Asp Leu Gly Thr Ala Asn Ala His Gln Leu Ala Leu 145 150 155 160 Gly Gln Phe Glu Met His Asn Glu Ala Ile Lys Ser Ala Ser Arg Ile 165 170 175 Leu Tyr Ser Gln Leu Ala Glu Thr Ile Val Gln Gln Val Tyr Tyr Asp 180 185 190 Leu Phe 58 585 DNA Chlamydia trachomatis 58 atgctgaaaa tgttttggtt gaatagcctc gttttcttct cgttactact atcagcctgc 60 ggctatacag tgctctcccc ccactatgta gaaaagaaat tctcgctttc cgaaggcatc 120 tatgtctgcc ctatcgaagg agattcatta ggagatctcg tatcctctct ttcttacgaa 180 ttagaaaagc gaggactcca cacacgatct caaggaacct cttctggtta tgtactcaaa 240 gtctctcttt tcaatgagac tgatgaaaat attggattcg catacactcc ccaaaaacct 300 gatgaaaaac ctgtaaaaca cttcattgtc tctaatgaag ggcgcttagc gttatcagca 360 aaagtccaac taatcaaaaa ccgcacacaa gaaatattag tggagaaatg cctgagaaaa 420 tcggttactt ttgattttca acctgacctc ggaaccgcga atgctcatca gctagctctc 480 ggacaatttg aaatgcataa tgaagcaata aaaagcgctt ctcgtatatt gtattcgcaa 540 ttagcagaga ctattgtaca acaggtatac tatgaccttt tctga 585 59 542 PRT Chlamydia trachomatis 59 Met Phe Lys Leu Val Ser Tyr Ile Ile Leu Ser Trp Val Leu Val Cys 1 5 10 15 Leu Ala Gln Pro Asp Val Ser Val Val Ala Ser Val Val Ser Cys Ile 20 25 30 Cys Gly Tyr Ser Leu Leu Trp Ala Gly Leu Phe Ala Leu Val Glu Gln 35 40 45 Leu Ser Trp Lys Lys Val Trp Cys Ile Ala Phe Ile Trp Thr Trp Thr 50 55 60 Val Glu Gly Ala His Phe Ser Trp Met Leu Glu Asp Leu Tyr Val Gly 65 70 75 80 Thr Ser Ile Tyr Phe Val Trp Gly Ile Leu Leu Ser Tyr Leu Ala Thr 85 90 95 Leu Phe Ala Ser Phe Ser Cys Leu Val Val Trp Cys Cys Arg Lys Gln 100 105 110 Tyr Arg Gly Ala Leu Val Trp Leu Pro Gly Val Trp Val Ala Ile Glu 115 120 125 Ala Ile Arg Tyr Tyr Gly Leu Leu Ser Gly Val Ser Phe Asp Phe Ile 130 135 140 Gly Trp Pro Leu Thr Ala Thr Ala Tyr Gly Arg Gln Phe Gly Ser Phe 145 150 155 160 Phe Gly Trp Ala Gly Gln Ser Phe Leu Val Ile Ala Ala Asn Ile Cys 165 170 175 Cys Phe Ala Val Cys Leu Leu Lys His Ser Phe Ser Lys Gly Leu Trp 180 185 190 Leu Thr Leu Cys Ala Phe Pro Tyr Leu Leu Gly Gly Ala His Tyr Glu 195 200 205 Tyr Leu Lys Lys His Phe Ser Asp Ser Glu Val Leu Arg Val Ala Ile 210 215 220 Val Gln Pro Gly Tyr Ser Pro His Met His Ala Gly Arg Thr Ala Ser 225 230 235 240 Ala Ile Trp Arg Gly Leu Val Ser Leu Cys Gln Thr Ile Gln Thr Pro 245 250 255 Val Asp Val Ile Val Phe Pro Glu Val Ser Val Pro Phe Gly Leu His 260 265 270 Arg Gln Ala Tyr Thr Leu His Glu Asn Gln Pro Val Leu Glu Ser Leu 275 280 285 Leu Pro Asn Lys Ser Trp Gly Glu Phe Phe Thr Asn Leu Asp Trp Ile 290 295 300 Gln Ala Ile Ala Glu Arg Tyr Gln Cys Thr Val Ile Met Gly Met Glu 305 310 315 320 Arg Trp Glu Asn Lys Gly Gly Ile Leu His Leu Tyr Asn Ala Ala Glu 325 330 335 Cys Val Ser Arg Glu Gly Glu Ile Thr Ser Tyr Asp Lys Arg Ile Leu 340 345 350 Val Pro Gly Gly Glu Tyr Ile Pro Gly Gly Lys Ile Gly Phe Ser Leu 355 360 365 Cys Gln Thr Phe Phe Pro Glu Phe Ala Leu Pro Phe Gln Arg Leu Pro 370 375 380 Gly Glu Phe Ser Gly Val Val Asn Ile Thr Glu Arg Ile Lys Ala Gly 385 390 395 400 Ile Ser Ile Cys Tyr Glu Glu Thr Phe Gly Tyr Ala Ile Arg Pro Tyr 405 410 415 Lys Arg Gln Gln Ala Asp Ile Leu Val Asn Leu Thr Asn Asp Gly Trp 420 425 430 Tyr Pro Arg Ser Arg Leu Pro Leu Val His Phe Tyr His Gly Met Leu 435 440 445 Arg Asn Gln Glu Leu Gly Ile Pro Cys Ile Arg Ala Cys Arg Thr Gly 450 455 460 Val Ser Ala Ala Val Asp Ser Leu Gly Arg Ile Val Gly Ile Leu Pro 465 470 475 480 Trp Glu Ser Arg Thr Cys Pro Val Ser Thr Gly Val Leu Gln Val Ser 485 490 495 Val Pro Leu Tyr Ser Tyr His Thr Val Tyr Ala Arg Leu Gly Asp Ala 500 505 510 Pro Leu Leu Leu Ile Ala Val Cys Ser Val Ile Gly Ala Ile Ala Tyr 515 520 525 Phe Tyr Arg Lys Lys Lys Glu Thr Pro Pro Gln Thr Phe Phe 530 535 540 60 1629 DNA Chlamydia trachomatis 60 gtgtttaaac ttgtgtcata catcatcctt tcttgggtgc tggtctgttt ggctcagccg 60 gatgtaagtg ttgtagcttc tgttgttagt tgtatttgcg gttacagctt actttgggct 120 gggctttttg ctttagtaga gcaattatct tggaagaaag tttggtgcat cgcttttatt 180 tggacttgga ctgtcgaagg cgctcatttc tcttggatgc ttgaagatct ttatgtaggg 240 acaagcatct attttgtttg gggtatactg ctttcttatc tcgccaccct atttgctagt 300 ttttcttgtt tggttgtgtg gtgttgtcgc aagcaatata ggggagctct tgtttggctt 360 ccaggggttt gggtggcgat agaagcaata cgctattatg ggttgctttc aggagtttct 420 tttgatttta ttggctggcc tcttacagcg acagcctatg gccggcaatt cggcagcttt 480 tttggatggg ctggacaaag ctttctagtt attgctgcca atatatgctg ttttgcagta 540 tgtttattaa aacactcttt ttccaaaggt ttgtggttga cgttgtgcgc gttcccttat 600 ctgttaggcg gagcgcatta cgaataccta aagaagcatt tttccgactc tgaagtgctt 660 cgagttgcca tcgtgcagcc tggatatagt cctcatatgc atgcagggag gacggctagt 720 gctatttgga gaggtttggt ttctttgtgc cagactattc aaactcctgt agatgtgatc 780 gttttcccag aagtaagtgt tccttttggc ttacatagac aagcctatac tcttcatgaa 840 aatcagcctg tattagaaag tttgcttcct aacaaatctt ggggcgagtt tttcacaaat 900 ttggattgga tccaagcgat agctgaacgt tatcaatgca ccgttatcat gggaatggaa 960 cgatgggaaa ataaaggggg aatactgcat ttgtataatg ctgctgaatg cgtatcgcga 1020 gaaggggaaa taactagcta tgataagcgg attcttgttc ctggaggtga gtacatccct 1080 ggagggaaaa taggtttttc cttgtgtcaa acctttttcc cagaatttgc tcttcccttt 1140 caacgtttgc caggagagtt ttctggagtt gtgaatataa cagagcgaat aaaagctggg 1200 atctctattt gttatgagga gacatttggg tatgcaattc gcccttacaa aaggcaacaa 1260 gccgatattt tagtaaatct tactaatgac ggttggtatc cgcgttcaag gctgcctcta 1320 gtacattttt atcatggcat gttacgtaat caagagttgg gtataccttg tattcgcgcc 1380 tgtcgcacag gagtttctgc tgcagtggat tctttgggta gaattgtcgg catacttccc 1440 tgggaatcga gaacttgccc agtttctaca ggagtactcc aagtttccgt ccctctttac 1500 agttatcata ctgtatatgc aaggctgggt gatgctcctc tgttactgat tgcagtttgt 1560 tcggttatcg gagcgattgc ctatttttat aggaaaaaga aagagacccc accacaaaca 1620 tttttttga 1629 61 553 PRT Chlamydia trachomatis 61 Met Ser Ser Asn Lys His Ala Ser Leu Cys Gln Lys Thr Pro Ser Leu 1 5 10 15 Cys Arg Glu Leu Gln Lys Ala Pro Ala Leu Leu Leu Thr Glu Asp Ile 20 25 30 Arg Phe Lys Ala Leu Leu Asn Glu Arg Ile Asp Ser Val Ala Glu Leu 35 40 45 Phe Pro Cys Thr Tyr Asn Ser Pro Tyr Tyr Lys Phe Ile Ser Lys Ser 50 55 60 Asp Leu Ser Ala Glu Thr Ser Pro Leu Lys Val Gly Val Met Leu Ser 65 70 75 80 Gly Gly Pro Ala Pro Gly Gly His Asn Val Ile Leu Gly Leu Leu His 85 90 95 Ser Ile Lys Lys Leu His Pro Asn Ser Gln Leu Leu Gly Phe Ile Arg 100 105 110 Asn Gly Glu Gly Leu Leu Asn Asn Asn Thr Val Glu Ile Thr Asp Glu 115 120 125 Phe Ile Glu Glu Phe Arg Asn Ser Gly Gly Phe Asn Cys Ile Gly Thr 130 135 140 Gly Arg Thr Asn Ile Ile Thr Glu Glu Asn Lys Ala Arg Cys Leu Gln 145 150 155 160 Thr Ala Asn Glu Leu Asp Leu Asp Gly Leu Val Ile Ile Gly Gly Asp 165 170 175 Gly Ser Asn Thr Ala Thr Ala Ile Leu Ala Glu Tyr Phe Ala Lys His 180 185 190 Gln Ala Lys Thr Val Leu Val Gly Val Pro Lys Thr Ile Asp Gly Asp 195 200 205 Leu Gln His Leu Phe Leu Asp Leu Thr Phe Gly Phe Asp Thr Ala Thr 210 215 220 Lys Phe Tyr Ser Ser Ile Ile Ser Asn Ile Ser Arg Asp Ala Leu Ser 225 230 235 240 Cys Lys Gly His Tyr His Phe Ile Lys Leu Met Gly Arg Ser Ser Ser 245 250 255 His Ile Thr Leu Glu Cys Ala Leu Gln Thr His Pro Asn Ile Ala Leu 260 265 270 Ile Gly Glu Glu Ile Ala Glu Lys Ser Ile Ser Leu Glu Thr Leu Ile 275 280 285 His Asp Ile Cys Glu Thr Ile Ala Asp Arg Ala Ala Met Gly Lys Tyr 290 295 300 His Gly Val Ile Leu Ile Pro Glu Gly Val Ile Glu Phe Ile Pro Glu 305 310 315 320 Ile Gln Ser Leu Val Lys Glu Ile Glu Ser Ile Pro Glu Gln Glu Asn 325 330 335 Leu Tyr Gln Ala Leu Ser Leu Ser Ser Gln Gln Leu Leu Cys Gln Phe 340 345 350 Pro Glu Asp Ile Cys His Gln Leu Leu Tyr Asn Arg Asp Ala His Gly 355 360 365 Asn Val Tyr Val Ser Lys Ile Ser Val Asp Lys Leu Leu Ile His Leu 370 375 380 Val Arg Gln His Leu Glu Thr His Phe Arg Gln Val Pro Phe Asn Ala 385 390 395 400 Ile Ser His Phe Leu Gly Tyr Glu Gly Arg Ser Gly Thr Pro Thr His 405 410 415 Phe Asp Asn Val Tyr Ser Tyr Asn Leu Gly Tyr Gly Ala Gly Val Leu 420 425 430 Val Phe Asn Arg Cys Asn Gly Tyr Leu Ser Thr Ile Glu Gly Leu Thr 435 440 445 Ser Pro Ile Glu Lys Trp Arg Leu Arg Ala Leu Pro Ile Val Arg Met 450 455 460 Leu Thr Thr Lys Gln Gly Lys Asp Ser Lys His Tyr Pro Leu Ile Lys 465 470 475 480 Lys Arg Leu Val Asp Ile Ala Ser Pro Val Phe Asn Lys Phe Ser Leu 485 490 495 Tyr Arg Lys Ile Trp Ala Leu Glu Asp Ser Tyr Arg Phe Val Gly Pro 500 505 510 Leu Gln Ile His Ser Pro Glu Asp Ala His Ser Asp Asp Phe Pro Pro 515 520 525 Leu Ile Leu Phe Leu Asn His Asn Glu Trp Gln Lys Arg Cys Ser Ile 530 535 540 Cys Leu Glu Ile Pro Asp Gln Asp Tyr 545 550 62 1662 DNA Chlamydia trachomatis 62 atgtcgtcga ataaacatgc ttctctttgt caaaagacgc cttctttgtg tcgggagctt 60 caaaaagctc ctgctcttct attaacagaa gacataaggt ttaaagctct tcttaatgaa 120 cgcattgact ctgttgcaga actatttcca tgcacttata actctcccta ctacaaattt 180 atttcgaagt ccgatctttc cgctgagacc tctcccctta aagtgggcgt tatgctttct 240 ggaggcccag ctcctggtgg gcacaatgtc atcttaggat tgctacacag tattaaaaag 300 ctccatccga atagtcagct tttaggattt attcgcaatg gagaaggact tctcaataat 360 aatactgtag aaatcacaga tgaattcatt gaagagtttc gtaactctgg aggctttaat 420 tgcataggaa caggtcgcac taatatcata accgaagaaa ataaagcgcg ctgtttacaa 480 acagcaaatg aactcgattt agatggatta gtgattattg gaggcgatgg ttcgaataca 540 gccacggcga ttcttgctga atattttgct aagcatcaag caaaaacggt attagttggt 600 gttcccaaaa ctattgatgg agatttgcag cacctatttt tagacctcac atttgggttt 660 gatactgcta ctaaatttta ttcatccatc atcagcaaca tttctagaga cgcattatcg 720 tgtaaaggcc actatcattt tattaaacta atgggccggt cttcttctca tatcacgcta 780 gaatgcgcac tacagactca cccaaatatt gctcttatag gcgaagagat tgcagaaaaa 840 agcatctcct tagaaacatt aatccatgat atttgtgaaa caatagcaga tcgagctgct 900 atggggaaat accatggcgt tattctcatc cctgaaggag tcattgagtt tattcctgaa 960 atacagtctc tggttaaaga aattgaatcc attccagagc aggagaatct ttaccaagct 1020 ttatccttat cttctcagca acttttatgc caatttccgg aagatatttg ccatcagctc 1080 ttgtataata gagatgctca tggcaacgtc tatgtatcaa aaattagtgt tgataaactt 1140 ctgattcatc tagttcgtca acatttagaa acacatttta gacaagttcc cttcaatgca 1200 atctcccatt ttttaggtta tgaagggcgt tcaggaactc ctacacattt tgataatgtg 1260 tatagctata acttaggata tggtgctggg gttctcgttt ttaaccgctg taatgggtat 1320 ttatccacga tcgaaggtct aactagccct attgaaaaat ggcgattgcg cgctttaccc 1380 attgttcgaa tgttgacgac caagcagggg aaagacagta aacattatcc tctgataaaa 1440 aaaagattgg tagatattgc tagtcctgtt tttaataagt tctcactgta tcggaaaatc 1500 tgggctttag aagactccta tcgctttgta gggccattac aaatacattc tccggaggat 1560 gctcattctg atgattttcc tcctttaatt ttgtttttga atcataatga atggcaaaaa 1620 cgctgttcta tttgtttaga aatccccgat caggattatt aa 1662 63 167 PRT Chlamydia trachomatis 63 Met Ile Cys Cys Asp Lys Val Leu Ser Ser Val Gln Ser Met Pro Val 1 5 10 15 Ile Asp Lys Cys Ser Val Thr Lys Cys Leu Gln Thr Ala Lys Gln Ala 20 25 30 Ala Val Leu Ala Leu Ser Leu Phe Ala Val Phe Ala Ser Gly Ser Leu 35 40 45 Ser Ile Leu Ser Ala Ala Val Leu Phe Ser Gly Thr Ala Ala Val Leu 50 55 60 Pro Tyr Leu Leu Ile Leu Thr Thr Ala Leu Leu Gly Phe Val Cys Ala 65 70 75 80 Val Ile Val Leu Leu Arg Asn Leu Ser Ala Val Val Gln Ser Cys Lys 85 90 95 Lys Arg Ser Pro Glu Glu Ile Glu Gly Ala Ala Arg Pro Ser Asp Gln 100 105 110 Gln Glu Ser Gly Gly Arg Leu Ser Glu Glu Ser Ala Ser Pro Gln Ala 115 120 125 Ser Pro Thr Ser Ser Thr Phe Gly Leu Glu Ser Ala Leu Arg Ser Ile 130 135 140 Gly Asp Ser Val Ser Gly Ala Phe Asp Asp Ile Asn Lys Asp Asn Ser 145 150 155 160 Arg Ser Arg Ser His Ser Phe 165 64 439 DNA Chlamydia trachomatis 64 atgatctgct gtgacaaagt cttgtcgagc gtacaatcaa tgcctgttat agataaatgc 60 tctgtaacga aatgcttaca aacggctaag caagcagctg ttcttgcgtt gtctttgttt 120 gcggtgtttg cttcaggaag tttatccata ttatcagcgg cggtactgtt tagtggcact 180 gctgctgttc ttccatatct gctgatatta acaacagctc ttctaggatt tgtttgtgct 240 gttattgtgc ttttaagaaa tttatcagca gttgttcaga gttgtaaaaa gagatcacct 300 gaagaaattg aaggggctgc tcgtccctct gatcagcagg aatcaggagg acgtttgtcc 360 gaggagagcg cttcaccaca agcatctcct acttcgtcta cttttggtct tgaatccgct 420 ttgcgctcaa taggagata 439 65 89 PRT Chlamydia trachomatis 65 Met Ser Lys Lys Cys Ala Leu Thr Gly Arg Lys Pro Arg Arg Gly Tyr 1 5 10 15 Ser Tyr Ala Ile Arg Gly Ile Ser Lys Lys Lys Lys Gly Ile Gly Leu 20 25 30 Lys Val Thr Gly Arg Thr Lys Arg Arg Phe Phe Pro Asn Met Met Thr 35 40 45 Lys Arg Leu Trp Ser Thr Glu Glu Asn Arg Phe Leu Lys Leu Lys Ile 50 55 60 Ser Ala Ala Ala Leu Arg Leu Val Asp Lys Leu Gly Leu Asp Gln Val 65 70 75 80 Val Ala Arg Ala Lys Ser Lys Gly Phe 85 66 270 DNA Chlamydia trachomatis 66 atgtcgaaaa aatgtgcgct tacaggaaga aagcctcgtc gcggttatag ctatgctatc 60 cgagggattt ctaaaaagaa aaaagggatc ggtttgaaag ttacaggaag aacaaaacgt 120 cgattcttcc ctaatatgat gactaagaga ctatggtcta ctgaggaaaa tcgcttcctc 180 aaactcaaaa tttctgcagc agctttacgc cttgttgata aactagggtt agatcaggtt 240 gttgctagag ctaaaagcaa gggtttttag 270 67 157 PRT Chlamydia trachomatis 67 Met Leu Phe Gly Tyr Leu Val Gly Phe Leu Ala Ala Asp Pro Glu Glu 1 5 10 15 Arg Met Thr Ser Gly Gly Lys Arg Val Val Val Leu Arg Leu Gly Val 20 25 30 Lys Ser Arg Val Gly Ser Lys Asp Glu Thr Val Trp Cys Arg Cys Asn 35 40 45 Ile Trp Asn Asn Arg Tyr Asp Lys Met Leu Pro Tyr Leu Lys Lys Gly 50 55 60 Ser Ser Val Ile Val Ala Gly Glu Leu Ser Leu Glu Ser Tyr Val Gly 65 70 75 80 Arg Asp Gly Ser Pro Gln Ala Ser Ile Ser Val Ser Val Asp Thr Leu 85 90 95 Lys Phe Asn Ser Gly Ser Ser Arg Pro Asp Ala Arg Gly Ser Asp Glu 100 105 110 Gly Arg Gln Arg Ala Asn Asp Asn Val Ser Ile Gly Phe Asp Gly Glu 115 120 125 Ser Leu Asp Thr Asp Ser Ala Leu Asp Lys Glu Val Tyr Ala Gly Phe 130 135 140 Gly Glu Asp Gln Gln Tyr Ala Ser Glu Asp Val Pro Phe 145 150 155 68 474 DNA Chlamydia trachomatis 68 atgttgttcg gatatttggt aggatttcta gctgccgatc ctgaagaaag aatgacatcc 60 ggaggtaaac gggttgttgt tttacgtttg ggtgtaaaat ctcgtgtagg atctaaagat 120 gaaacagtgt ggtgcagatg caatatctgg aacaaccgtt atgataagat gcttccttat 180 ttgaagaaag gttcttcagt cattgttgct ggagagcttt ctttagaaag ctatgtaggt 240 agagacggtt ctccacaagc ttctatttct gtaagcgtag atacattaaa atttaattcc 300 ggatcttctc gtcctgatgc tagaggttca gatgaaggtc gtcagagagc taatgataat 360 gtctctattg gatttgatgg agaaagttta gatacagact ctgcgcttga taaggaagtc 420 tatgcagggt ttggagaaga ccaacagtat gctagtgagg atgttccttt ttag 474 69 247 PRT Chlamydia trachomatis 69 Met Lys Lys Gln Glu Lys Met His Pro Gln Asn Leu Leu Lys Val Phe 1 5 10 15 Ile Phe Phe Leu Ala Phe Phe Ile Ser Tyr Pro Ser Ala Glu Ala His 20 25 30 Ser Pro Leu Gln Ser Ser Ile Gln Glu Lys Ile Leu Thr Ala Arg Pro 35 40 45 Gly Asp Tyr Ala Val Leu Ser Arg Gly Ser Gln Lys Phe Phe Phe Leu 50 55 60 Ile Arg Gln Ser Ser Ser Glu Ala Thr Trp Val Glu Met Ser Glu Phe 65 70 75 80 Ala Ser Leu Thr Gln Gln Glu Lys Lys Leu Val Glu Gln Ser Ser Trp 85 90 95 Lys Asn Ala Phe His Gln Leu Gln Ser Ser Lys Lys Val Tyr Leu Leu 100 105 110 Arg Ile Ser Lys Asn Pro Leu Met Ile Phe Val Leu Lys Asn Ala Gln 115 120 125 Trp Met Pro Leu Ser Glu Lys Asp Pro Leu Pro Phe Phe Val Lys Ile 130 135 140 Leu Arg Leu Pro Leu Ser Pro Ala Pro Ser His Leu Ile Lys Tyr Lys 145 150 155 160 Gly Lys Glu Arg Thr Pro Trp Ser Pro Arg Thr Ser Leu Asn Gly Glu 165 170 175 Leu Ile Thr Leu Pro Ser Ser Ala Trp Ile Ser Val Trp Pro Lys Asp 180 185 190 Ser Ser Pro Leu Ser Glu Lys Asn Ile Leu Ile Tyr Phe Ser Asn Asn 195 200 205 Glu Arg Leu Ala Phe Pro Leu Trp Thr Ser Ile Asp Thr Pro Thr Gly 210 215 220 Thr Val Ile Ile Lys Thr Ile Glu Met Gly His Gln Ala Ala Ser Ser 225 230 235 240 Tyr Pro Ala Leu Pro Asn Phe 245 70 744 DNA Chlamydia trachomatis 70 atgaaaaagc aagaaaaaat gcaccctcaa aaccttctta aagtttttat ttttttcttg 60 gcatttttca tatcctatcc ctcggctgaa gcccattctc ctctccaatc atcaatccaa 120 gaaaaaattc taactgcccg ccccggagac tatgccgtct taagccgagg atctcaaaaa 180 tttttctttt taattcgcca aagttcttcg gaagcgactt gggtcgaaat gtctgaattt 240 gcctccctaa cacagcaaga aaaaaaatta gtagaacagt cttcctggaa gaatgccttc 300 catcaactcc aatcttcaaa aaaagtgtac ttgttacgaa tttccaaaaa tcctcttatg 360 atttttgttc tcaaaaatgc gcaatggatg cctctctcag aaaaagatcc tttgcctttc 420 tttgtaaaaa tccttcgact ccctttatct ccagccccct ctcacttaat taaatacaaa 480 gggaaagaac gcaccccctg gtctccgcga acatctttga atggagaact cataaccctt 540 ccttccagtg cttggatttc tgtttggcca aaagattctt ctcctctatc agaaaaaaat 600 attctcatat atttttctaa caatgaacgt ttagcgtttc ctctatggac tagtattgat 660 actcctacag ggacagtgat tattaagact attgaaatgg ggcaccaagc cgcctcctcc 720 tatccagctc ttcccaattt ctag 744 71 456 DNA Chlamydia trachomatis 71 aatatgagca ctgtacccgt tgttcaagga gctggatctt ccaattcggc acaggatatt 60 tccactagac cattaacact gaaagagcgt atatcgaatc ttctatcttc cactgcattt 120 aaggtgggat tagtggtgat aggactactt ttagtgattg ctactttgat attcctagtt 180 tcggcagctt cgtttgtaaa tgccatctat ctagtagcta ttcctgctat tttgggatgc 240 gtgaatatct gcgtaggaat tttatccatg gaaggacact gttctccgga gagatggatc 300 ttatgtaaga aggtattaaa gacttcagaa gatatcatcg atgatgggca gataaacaac 360 tctaataaag tgtttactga tgagaggttg aatgccatag gtggggtagt ggaatctcta 420 tctagaagaa atagtctggt ggatcagacc caatga 456 72 151 PRT Chlamydia trachomatis 72 Asn Met Ser Thr Val Pro Val Val Gln Gly Ala Gly Ser Ser Asn Ser 1 5 10 15 Ala Gln Asp Ile Ser Thr Arg Pro Leu Thr Leu Lys Glu Arg Ile Ser 20 25 30 Asn Leu Leu Ser Ser Thr Ala Phe Lys Val Gly Leu Val Val Ile Gly 35 40 45 Leu Leu Leu Val Ile Ala Thr Leu Ile Phe Leu Val Ser Ala Ala Ser 50 55 60 Phe Val Asn Ala Ile Tyr Leu Val Ala Ile Pro Ala Ile Leu Gly Cys 65 70 75 80 Val Asn Ile Cys Val Gly Ile Leu Ser Met Glu Gly His Cys Ser Pro 85 90 95 Glu Arg Trp Ile Leu Cys Lys Lys Val Leu Lys Thr Ser Glu Asp Ile 100 105 110 Ile Asp Asp Gly Gln Ile Asn Asn Ser Asn Lys Val Phe Thr Asp Glu 115 120 125 Arg Leu Asn Ala Ile Gly Gly Val Val Glu Ser Leu Ser Arg Arg Asn 130 135 140 Ser Leu Val Asp Gln Thr Gln 145 150 73 354 DNA Chlamydia trachomatis 73 gggctagttt cttttattgt taaaagaatt gcttttatcg ataaaagaaa cttcaagagc 60 ccttttctag aaaggagtct ggaagttatg aaaaaaactg ctttactcgc tgctttatgt 120 agtgttgttt ctttaagtag ttgttgtcgt atcgttgact gttgcttcga agatccatgc 180 gcacctatcc aatgttcacc ttgtgaatct aagaagaaag acgtagacgg tggttgcaac 240 tcttgtaacg ggtatgtccc agcttgcaaa ccttgcggag gggatacgca ccaagatgct 300 aaacatggcc ctcaagctag aggaattcca gttgacggca aatgcagaca atag 354 74 117 PRT Chlamydia trachomatis 74 Gly Leu Val Ser Phe Ile Val Lys Arg Ile Ala Phe Ile Asp Lys Arg 1 5 10 15 Asn Phe Lys Ser Pro Phe Leu Glu Arg Ser Leu Glu Val Met Lys Lys 20 25 30 Thr Ala Leu Leu Ala Ala Leu Cys Ser Val Val Ser Leu Ser Ser Cys 35 40 45 Cys Arg Ile Val Asp Cys Cys Phe Glu Asp Pro Cys Ala Pro Ile Gln 50 55 60 Cys Ser Pro Cys Glu Ser Lys Lys Lys Asp Val Asp Gly Gly Cys Asn 65 70 75 80 Ser Cys Asn Gly Tyr Val Pro Ala Cys Lys Pro Cys Gly Gly Asp Thr 85 90 95 His Gln Asp Ala Lys His Gly Pro Gln Ala Arg Gly Ile Pro Val Asp 100 105 110 Gly Lys Cys Arg Gln 115 75 1650 DNA Chlamydia trachomatis 75 gctagtaagg gagccccttt agtgtttaaa cttgtgtcat acatcatcct ttcttgggtg 60 ctggtctgtt tggctcagcc ggatgtaagt gttgtagctt ctgttgttag ttgtatttgc 120 ggttacagct tactttgggc tgggcttttt gctttagtag agcaattatc ttggaagaaa 180 gtttggtgca tcgcttttat ttggacttgg actgtcgaag gcgctcattt ctcttggatg 240 cttgaagatc tttatgtagg gacaagcatc tattttgttt ggggtatact gctttcttat 300 ctcgccaccc tatttgctag tttttcttgt ttggttgtgt ggtgttgtcg caagcaatat 360 aggggagctc ttgtttggct tccaggggtt tgggtggcga tagaagcaat acgctattat 420 gggttgcttt caggagtttc ttttgatttt attggctggc ctcttacagc gacagcctat 480 ggccggcaat tcggcagctt ttttggatgg gctggacaaa gctttctagt tattgctgcc 540 aatatatgct gttttgcagt atgtttatta aaacactctt tttccaaagg tttgtggttg 600 acgttgtgcg cgttccctta tctgttaggc ggagcgcatt acgaatacct aaagaagcat 660 ttttccgact ctgaagtgct tcgagttgcc atcgtgcagc ctggatatag tcctcatatg 720 catgcaggga ggacggctag tgctatttgg agaggtttgg tttctttgtg ccagactatt 780 caaactcctg tagatgtgat cgttttccca gaagtaagtg ttccttttgg cttacataga 840 caagcctata ctcttcatga aaatcagcct gtattagaaa gtttgcttcc taacaaatct 900 tggggcgagt ttttcacaaa tttggattgg atccaagcga tagctgaacg ttatcaatgc 960 accgttatca tgggaatgga acgatgggaa aataaagggg gaatactgca tttgtataat 1020 gctgctgaat gcgtatcgcg agaaggggaa ataactagct atgataagcg gattcttgtt 1080 cctggaggtg agtacatccc tggagggaaa ataggttttt ccttgtgtca aacctttttc 1140 ccagaatttg ctcttccctt tcaacgtttg ccaggagagt tttctggagt tgtgaatata 1200 acagagcgaa taaaagctgg gatctctatt tgttatgagg agacatttgg gtatgcaatt 1260 cgcccttaca aaaggcaaca agccgatatt ttagtaaatc ttactaatga cggttggtat 1320 ccgcgttcaa ggctgcctct agtacatttt tatcatggca tgttacgtaa tcaagagttg 1380 ggtatacctt gtattcgcgc ctgtcgcaca ggagtttctg ctgcagtgga ttctttgggt 1440 agaattgtcg gcatacttcc ctgggaatcg agaacttgcc cagtttctac aggagtactc 1500 caagtttccg tccctcttta cagttatcat actgtatatg caaggctggg tgatgctcct 1560 ctgttactga ttgcagtttg ttcggttatc ggagcgattg cctattttta taggaaaaag 1620 aaagagaccc caccacaaac atttttttga 1650 76 549 PRT Chlamydia trachomatis 76 Ala Ser Lys Gly Ala Pro Leu Val Phe Lys Leu Val Ser Tyr Ile Ile 1 5 10 15 Leu Ser Trp Val Leu Val Cys Leu Ala Gln Pro Asp Val Ser Val Val 20 25 30 Ala Ser Val Val Ser Cys Ile Cys Gly Tyr Ser Leu Leu Trp Ala Gly 35 40 45 Leu Phe Ala Leu Val Glu Gln Leu Ser Trp Lys Lys Val Trp Cys Ile 50 55 60 Ala Phe Ile Trp Thr Trp Thr Val Glu Gly Ala His Phe Ser Trp Met 65 70 75 80 Leu Glu Asp Leu Tyr Val Gly Thr Ser Ile Tyr Phe Val Trp Gly Ile 85 90 95 Leu Leu Ser Tyr Leu Ala Thr Leu Phe Ala Ser Phe Ser Cys Leu Val 100 105 110 Val Trp Cys Cys Arg Lys Gln Tyr Arg Gly Ala Leu Val Trp Leu Pro 115 120 125 Gly Val Trp Val Ala Ile Glu Ala Ile Arg Tyr Tyr Gly Leu Leu Ser 130 135 140 Gly Val Ser Phe Asp Phe Ile Gly Trp Pro Leu Thr Ala Thr Ala Tyr 145 150 155 160 Gly Arg Gln Phe Gly Ser Phe Phe Gly Trp Ala Gly Gln Ser Phe Leu 165 170 175 Val Ile Ala Ala Asn Ile Cys Cys Phe Ala Val Cys Leu Leu Lys His 180 185 190 Ser Phe Ser Lys Gly Leu Trp Leu Thr Leu Cys Ala Phe Pro Tyr Leu 195 200 205 Leu Gly Gly Ala His Tyr Glu Tyr Leu Lys Lys His Phe Ser Asp Ser 210 215 220 Glu Val Leu Arg Val Ala Ile Val Gln Pro Gly Tyr Ser Pro His Met 225 230 235 240 His Ala Gly Arg Thr Ala Ser Ala Ile Trp Arg Gly Leu Val Ser Leu 245 250 255 Cys Gln Thr Ile Gln Thr Pro Val Asp Val Ile Val Phe Pro Glu Val 260 265 270 Ser Val Pro Phe Gly Leu His Arg Gln Ala Tyr Thr Leu His Glu Asn 275 280 285 Gln Pro Val Leu Glu Ser Leu Leu Pro Asn Lys Ser Trp Gly Glu Phe 290 295 300 Phe Thr Asn Leu Asp Trp Ile Gln Ala Ile Ala Glu Arg Tyr Gln Cys 305 310 315 320 Thr Val Ile Met Gly Met Glu Arg Trp Glu Asn Lys Gly Gly Ile Leu 325 330 335 His Leu Tyr Asn Ala Ala Glu Cys Val Ser Arg Glu Gly Glu Ile Thr 340 345 350 Ser Tyr Asp Lys Arg Ile Leu Val Pro Gly Gly Glu Tyr Ile Pro Gly 355 360 365 Gly Lys Ile Gly Phe Ser Leu Cys Gln Thr Phe Phe Pro Glu Phe Ala 370 375 380 Leu Pro Phe Gln Arg Leu Pro Gly Glu Phe Ser Gly Val Val Asn Ile 385 390 395 400 Thr Glu Arg Ile Lys Ala Gly Ile Ser Ile Cys Tyr Glu Glu Thr Phe 405 410 415 Gly Tyr Ala Ile Arg Pro Tyr Lys Arg Gln Gln Ala Asp Ile Leu Val 420 425 430 Asn Leu Thr Asn Asp Gly Trp Tyr Pro Arg Ser Arg Leu Pro Leu Val 435 440 445 His Phe Tyr His Gly Met Leu Arg Asn Gln Glu Leu Gly Ile Pro Cys 450 455 460 Ile Arg Ala Cys Arg Thr Gly Val Ser Ala Ala Val Asp Ser Leu Gly 465 470 475 480 Arg Ile Val Gly Ile Leu Pro Trp Glu Ser Arg Thr Cys Pro Val Ser 485 490 495 Thr Gly Val Leu Gln Val Ser Val Pro Leu Tyr Ser Tyr His Thr Val 500 505 510 Tyr Ala Arg Leu Gly Asp Ala Pro Leu Leu Leu Ile Ala Val Cys Ser 515 520 525 Val Ile Gly Ala Ile Ala Tyr Phe Tyr Arg Lys Lys Lys Glu Thr Pro 530 535 540 Pro Gln Thr Phe Phe 545 77 645 DNA Chlamydia trachomatis 77 gaaaattgtt ataggatcag gagagaaacg tttcccatgc tggggagcat ttccttcaca 60 acatataaag aaaacctcat gagaaagact atttttaaag cgtttaattt attattctcc 120 cttctttttc tttcttcatg ctcttatcct tgcagagatt gggaatgcca tggttgcgac 180 tccgcaagac ctcgtaaatc ctcttttgga ttcgtacctt tctactccga tgaagaaatt 240 caacaagctt ttgttgaaga ttttgattcc aaagaagagc agctgtacaa aacgagcgca 300 cagagtacct ctttccgaaa tatcactttc gctacagata gttattctat taaaggagag 360 gataacctca cgattcttgc aagcttagtt cgtcatttgc ataaatctcc taaagctacg 420 ctatatatag agggccatac agatgaacgt ggagctgcag cttataacct agctttagga 480 gctcgtcgtg cgaatgctgt aaaacaatac ctcatcaaac agggaatcgc tgcagaccgc 540 ttattcacta tttcttacgg aaaagaacat cctgttcatc caggccataa tgaattagct 600 tggcaacaaa atcgtcgtac tgaatttaag atccatgctc gctaa 645 78 214 PRT Chlamydia trachomatis 78 Glu Asn Cys Tyr Arg Ile Arg Arg Glu Thr Phe Pro Met Leu Gly Ser 1 5 10 15 Ile Ser Phe Thr Thr Tyr Lys Glu Asn Leu Met Arg Lys Thr Ile Phe 20 25 30 Lys Ala Phe Asn Leu Leu Phe Ser Leu Leu Phe Leu Ser Ser Cys Ser 35 40 45 Tyr Pro Cys Arg Asp Trp Glu Cys His Gly Cys Asp Ser Ala Arg Pro 50 55 60 Arg Lys Ser Ser Phe Gly Phe Val Pro Phe Tyr Ser Asp Glu Glu Ile 65 70 75 80 Gln Gln Ala Phe Val Glu Asp Phe Asp Ser Lys Glu Glu Gln Leu Tyr 85 90 95 Lys Thr Ser Ala Gln Ser Thr Ser Phe Arg Asn Ile Thr Phe Ala Thr 100 105 110 Asp Ser Tyr Ser Ile Lys Gly Glu Asp Asn Leu Thr Ile Leu Ala Ser 115 120 125 Leu Val Arg His Leu His Lys Ser Pro Lys Ala Thr Leu Tyr Ile Glu 130 135 140 Gly His Thr Asp Glu Arg Gly Ala Ala Ala Tyr Asn Leu Ala Leu Gly 145 150 155 160 Ala Arg Arg Ala Asn Ala Val Lys Gln Tyr Leu Ile Lys Gln Gly Ile 165 170 175 Ala Ala Asp Arg Leu Phe Thr Ile Ser Tyr Gly Lys Glu His Pro Val 180 185 190 His Pro Gly His Asn Glu Leu Ala Trp Gln Gln Asn Arg Arg Thr Glu 195 200 205 Phe Lys Ile His Ala Arg 210 79 943 PRT Neisseria gonorrhoeae 79 Met Asn Lys Lys His Gly Phe Pro Leu Thr Leu Thr Ala Leu Ala Ile 1 5 10 15 Ala Thr Ala Phe Pro Ala Tyr Ala Ala Gln Ala Gly Ala Ala Ala Leu 20 25 30 Asp Ala Ala Gln Ser Gln Ser Leu Lys Glu Val Thr Val Arg Ala Ala 35 40 45 Lys Val Gly Arg Arg Ser Lys Glu Ala Thr Gly Leu Gly Lys Ile Val 50 55 60 Lys Thr Ser Glu Thr Leu Asn Lys Glu Gln Val Leu Gly Ile Arg Asp 65 70 75 80 Leu Thr Arg Tyr Asp Pro Gly Val Ala Val Val Glu Gln Gly Asn Gly 85 90 95 Ala Ser Gly Gly Tyr Ser Ile Arg Gly Val Asp Lys Asn Arg Val Ala 100 105 110 Val Ser Val Asp Gly Val Ala Gln Ile Gln Ala Phe Thr Val Gln Gly 115 120 125 Ser Leu Ser Gly Tyr Gly Gly Arg Gly Gly Ser Gly Ala Ile Asn Glu 130 135 140 Ile Glu Tyr Glu Asn Ile Ser Thr Val Glu Ile Asp Lys Gly Ala Gly 145 150 155 160 Ser Ser Asp His Gly Ser Gly Ala Leu Gly Gly Ala Val Ala Phe Arg 165 170 175 Thr Lys Glu Ala Ala Asp Leu Ile Ser Asp Gly Lys Ser Trp Gly Ile 180 185 190 Gln Ala Lys Thr Ala Tyr Gly Ser Lys Asn Arg Gln Phe Met Lys Ser 195 200 205 Leu Gly Ala Gly Phe Ser Lys Asp Gly Trp Glu Gly Leu Leu Ile Arg 210 215 220 Thr Glu Arg Gln Gly Arg Glu Thr Arg Pro His Gly Asp Ile Ala Asp 225 230 235 240 Gly Val Glu Tyr Gly Ile Asp Arg Leu Asp Ala Phe Arg Gln Thr Tyr 245 250 255 Asp Ile Lys Arg Lys Thr Thr Glu Pro Phe Phe Leu Val Glu Gly Glu 260 265 270 Asn Thr Leu Lys Pro Val Ala Lys Leu Ala Gly Tyr Gly Ile Tyr Leu 275 280 285 Asn Arg Gln Leu Asn Arg Trp Val Lys Glu Arg Ile Glu Gln Asn Gln 290 295 300 Pro Leu Ser Ala Glu Glu Glu Ala Gln Val Arg Glu Ala Gln Ala Arg 305 310 315 320 His Glu Asn Leu Ser Ala Gln Ala Tyr Thr Gly Gly Gly Arg Ile Leu 325 330 335 Pro Asp Pro Met Asp Tyr Arg Ser Gly Ser Trp Leu Ala Lys Leu Gly 340 345 350 Tyr Arg Phe Gly Gly Arg His Tyr Val Gly Gly Val Phe Glu Asp Thr 355 360 365 Lys Gln Arg Tyr Asp Ile Arg Asp Met Thr Glu Lys Gln Tyr Tyr Gly 370 375 380 Thr Asp Glu Ala Glu Lys Phe Arg Asp Lys Ser Gly Val Tyr Asp Gly 385 390 395 400 Asp Asp Phe Arg Asp Gly Leu Tyr Phe Val Pro Asn Ile Glu Glu Trp 405 410 415 Lys Gly Asp Lys Asn Leu Val Lys Gly Ile Gly Leu Lys Tyr Ser Arg 420 425 430 Thr Lys Phe Ile Asp Glu His His Arg Arg Arg Arg Met Gly Leu Leu 435 440 445 Tyr Arg Tyr Glu Asn Glu Lys Tyr Ser Asp Asn Trp Ala Asp Lys Ala 450 455 460 Val Leu Ser Phe Asp Lys Gln Gly Val Ala Thr Asp Asn Asn Thr Leu 465 470 475 480 Lys Leu Asn Cys Ala Val Tyr Pro Ala Val Asp Lys Ser Cys Arg Ala 485 490 495 Ser Ala Asp Lys Pro Tyr Ser Tyr Asp Ser Ser Asp Arg Phe His Tyr 500 505 510 Arg Glu Gln His Asn Val Leu Asn Ala Ser Phe Glu Lys Ser Leu Lys 515 520 525 Asn Lys Trp Thr Lys His His Leu Thr Leu Gly Phe Gly Tyr Asp Ala 530 535 540 Ser Lys Ala Val Ser Arg Pro Glu Gln Leu Ser His Asn Ala Ala Arg 545 550 555 560 Ile Ser Glu Ser Thr Gly Phe Asp Glu Lys Asn Gln Asp Lys Tyr Arg 565 570 575 Leu Gly Lys Pro Glu Val Val Glu Gly Ser Val Cys Gly Tyr Ile Glu 580 585 590 Thr Leu Arg Ser Arg Lys Cys Val Pro Arg Lys Ile Asn Gly Ser Asn 595 600 605 Ile His Ile Ser Leu Asn Asp Arg Phe Ser Ile Gly Lys Tyr Phe Asp 610 615 620 Phe Ser Leu Gly Gly Arg Tyr Asp Arg Lys Asn Phe Thr Thr Ser Glu 625 630 635 640 Glu Leu Val Arg Ser Gly Arg Tyr Ala Asp Arg Ser Trp Asn Ser Gly 645 650 655 Ile Val Phe Lys Pro Asn Arg His Phe Ser Val Ser Tyr Arg Ala Ser 660 665 670 Ser Gly Phe Arg Thr Pro Ser Phe Gln Glu Leu Phe Gly Ile Asp Ile 675 680 685 Tyr His Asp Tyr Pro Lys Gly Trp Gln Arg Pro Ala Leu Lys Ser Glu 690 695 700 Lys Ala Ala Asn Arg Glu Ile Gly Leu Gln Trp Lys Gly Asp Phe Gly 705 710 715 720 Phe Leu Glu Ile Ser Ser Phe Arg Asn Arg Tyr Thr Asp Met Ile Ala 725 730 735 Val Ala Asp Gln Lys Thr Lys Leu Pro Asp Ser Ala Gly Arg Leu Thr 740 745 750 Glu Ile Asp Ile Arg Asp Tyr Tyr Asn Ala Gln Asn Met Ser Leu Gln 755 760 765 Gly Ile Asn Ile Leu Gly Lys Ile Asp Trp Asn Gly Val Tyr Gly Lys 770 775 780 Leu Pro Glu Gly Leu Tyr Thr Thr Leu Ala Tyr Asn Arg Ile Lys Pro 785 790 795 800 Lys Ser Val Ser Asn Arg Pro Asp Leu Ser Leu Arg Ser Tyr Ala Leu 805 810 815 Asp Ala Val Gln Pro Ser Arg Tyr Val Leu Gly Phe Gly Tyr Asp Gln 820 825 830 Pro Glu Gly Lys Trp Gly Ala Asn Ile Met Leu Thr Tyr Ser Lys Gly 835 840 845 Lys Asn Pro Asp Glu Leu Ala Tyr Leu Ala Gly Asp Gln Lys Arg Tyr 850 855 860 Ser Ala Gly Arg Val Thr Ser Ser Trp Lys Thr Ala Asp Val Ser Ala 865 870 875 880 Tyr Leu Asn Leu Lys Lys Arg Leu Thr Leu Arg Ala Ala Ile Tyr Asn 885 890 895 Ile Gly Asn Tyr Arg Tyr Val Thr Trp Glu Ser Leu Arg Gln Thr Ala 900 905 910 Glu Ser Thr Ala Asn Arg His Gly Gly Asp Ser Asn Tyr Gly Arg Tyr 915 920 925 Ala Ala Pro Gly Arg Asn Phe Ser Leu Ala Leu Glu Met Lys Phe 930 935 940 80 3300 DNA Neisseria gonorrhoeae 80 ctcgggataa cggcatcaat ctttcgggaa atggttcgac taatcctcaa agtttcaaag 60 ccgacaatct tcttgtaacg ggcggctttt acggcccgca ggcggcggaa ttgggcggca 120 ctattttcaa taaggatggg aaatctcttg gtataactga agatattgaa aatgaagttg 180 aaaatgaagc tgatgttggc gaacagttag aacctgaagt taaaccccaa ttcggcgtgg 240 tattcggtgc gaagaaagat aataaagagg tggaaaaatg aataagaaac acggttttcc 300 gctgactttg acggcgttgg ccattgcaac cgcttttccg gcttatgctg cccaagcggg 360 ggcggcggca cttgatgcgg cgcaaagtca atcattgaaa gaggttaccg tccgtgccgc 420 caaagtggga cggcgatcga aagaggcgac aggtttgggc aaaatcgtca aaacgtcgga 480 aacgttgaac aaagaacagg tactcggtat ccgcgacctg acgcgctacg atccgggcgt 540 ggcggttgtc gaacagggca acggcgcgag cggcggctac tcgatacgcg gcgtagataa 600 aaaccgtgtg gcggtttcgg ttgacggcgt tgcccaaata caggcgttta ccgtgcaggg 660 atcgttgagc ggatacggcg gacgcggcgg cagcggcgca atcaacgaaa tcgaatatga 720 aaacatcagc acggtggaaa tcgacaaagg cgccggttcg tccgatcacg gcagcggcgc 780 actcggcggc gcggtcgcct tccgcaccaa agaggcggca gacctgattt cagacggcaa 840 aagctggggg atacaggcaa aaaccgctta cggcagtaaa aaccgccaat ttatgaagtc 900 gctcggcgcg gggttcagca aagacggttg ggaagggctg ctaatccgaa ccgaacgcca 960 agggcgggaa acgcgcccgc acggcgatat tgcggacggg gtggaatacg gcatagaccg 1020 tttggacgcg ttccgccaga catacgatat taaacgcaag acaacagagc catttttctt 1080 agtagagggc gagaatacac tcaagcccgt ggcaaaattg gcgggctacg ggatatattt 1140 gaaccgccag ctcaaccgct gggtaaaaga acgtattgaa caaaatcagc ctttaagtgc 1200 tgaagaagag gcgcaggtgc gggaggcgca ggcgcgccac gaaaacctgt ccgcccaagc 1260 ctacacgggc ggcggcagga tattgcccga tccgatggat taccgcagcg gctcttggct 1320 tgccaagctg ggctaccgct tcggcggcag gcattatgtc ggcggcgtgt ttgaggatac 1380 caaacagcgt tacgacatcc gcgatatgac ggaaaaacag tattacggta cggacgaggc 1440 ggaaaagttt agagacaaga gcggggtgta cgacggcgac gatttccgcg acggcttgta 1500 ttttgtgccg aatatagaag agtggaaggg cgataaaaat ttggtcaagg gcataggttt 1560 gaaatattcc cgcaccaaat ttattgacga acatcaccgc cgccgccgta tgggtttgct 1620 gtaccgttat gaaaatgaga aatactcgga caactgggcg gataaggcgg tgttgtcgtt 1680 tgacaaacag ggcgtggcaa ccgacaacaa cacgctgaag ctgaattgcg ccgtgtatcc 1740 tgccgtggac aaatcctgcc gcgcgtcggc ggacaaaccg tattcctacg acagcagcga 1800 ccgtttccac taccgcgaac agcacaatgt tttgaatgcc tcgtttgaga agtcgctgaa 1860 aaacaaatgg acgaaacacc atctgacttt gggcttcggt tacgatgctt ccaaagcagt 1920 atcccgccca gaacagcttt cccacaatgc ggcaaggatt tcggaatcca cgggattcga 1980 tgaaaagaat caagataagt accgtttggg taagcccgaa gtcgtcgaag ggtcggtctg 2040 cggctatatc gaaaccctgc gttcccgcaa atgcgtgcca agaaaaatca acggcagcaa 2100 tatccacatt tctttgaacg accgtttttc aatcggcaaa tattttgatt tcagcttggg 2160 cggcaggtac gaccggaaaa acttcaccac gtcggaagaa ctcgtccgca gcgggcggta 2220 tgccgaccgt tcgtggaaca gcggcatcgt gttcaaaccg aaccggcatt tttccgtgtc 2280 ttaccgcgcc tccagcggct tcagaacgcc ttccttccaa gaacttttcg ggatagacat 2340 ttatcacgat tatccgaaag gctggcagcg tcccgccctg aaatcggaaa aggcagccaa 2400 ccgggaaatc ggtttgcagt ggaagggcga tttcggcttt ttggaaatca gcagtttccg 2460 caaccgttat accgatatga ttgccgttgc cgatcaaaaa accaaattgc cggattcagc 2520 aggacgattg acagagattg atatacgcga ttattacaat gcccaaaata tgtcgcttca 2580 aggcatcaac atcttgggga aaatcgactg gaacggcgta tacggcaaac tgcccgaagg 2640 cctgtacacc acattggcgt acaaccgtat caaaccgaaa tcggtatcca accggccgga 2700 cttgtccctc cgcagctatg ctttggatgc ggtacagccg tcgcgttatg ttttggggtt 2760 cggatacgac cagcccgagg ggaaatgggg cgcaaacatt atgctgacct attccaaagg 2820 gaaaaaccct gacgagcttg cttatctggc aggcgatcaa aaacgatatt cggcaggaag 2880 ggttacgtct tcttggaaaa cggcagatgt ttccgcttat ctgaatctga aaaaacggct 2940 gaccttgagg gcggctatct acaatatcgg caactaccgc tacgttactt gggaatcctt 3000 gcgccagact gcggaaagca cggcaaaccg gcacggcggc gacagcaact atggaaggta 3060 tgccgcaccg ggcaggaact tcagcctcgc gctcgaaatg aagttttaaa ggaaatgccg 3120 tctggaagct tgatctgcac cccaaaagtc ggactaaacc gccaactgat taaggtgcag 3180 gtttttttga ttcaatataa acaagatttc cgccgtcatt cccgcgcagg cgggaatccg 3240 gacattcaat gctaaggcaa tttatcggaa atgactgaaa ctcaaaaaac cggattccca 3300 81 728 PRT Neisseria gonorrhoeae 81 Met Arg Lys Leu Asn Tyr Tyr Gly Ile Ala Leu Leu Pro Leu Met Leu 1 5 10 15 Ala Ser Cys Gly Gly Asn Phe Gly Val Gln Pro Val Val Glu Ser Thr 20 25 30 Pro Thr Ala Tyr Pro Val Thr Phe Lys Ser Lys Asp Val Pro Thr Ser 35 40 45 Pro Pro Pro Ala Glu Pro Ser Val Glu Thr Thr Pro Val Asn Arg Pro 50 55 60 Ala Val Gly Ala Ala Met Arg Leu Leu Arg Arg Asn Thr Ala Phe His 65 70 75 80 Arg Glu Asp Gly Thr Ala Ile Pro Asp Ser Lys Gln Ala Glu Glu Lys 85 90 95 Leu Ser Phe Lys Glu Gly Asp Val Leu Phe Leu Tyr Gly Ser Lys Gly 100 105 110 Asn Lys Leu Gln Gln Leu Lys Ser Glu Ile His Lys Arg Asp Ser Asp 115 120 125 Val Glu Ile Arg Thr Ser Glu Lys Glu Asn Lys Lys Tyr Gly Tyr Glu 130 135 140 Phe Val Asp Ala Gly Tyr Val Tyr Thr Lys Asn Gly Lys Asp Glu Ile 145 150 155 160 Glu Gln Asn Ser Gly Gly Lys Arg Phe Thr His Arg Phe Gly Tyr Asp 165 170 175 Gly Phe Val Tyr Tyr Ser Gly Glu Arg Pro Ser Gln Ser Leu Pro Ser 180 185 190 Ala Gly Thr Val Lys Tyr Phe Gly Asn Trp Gln Tyr Met Thr Asp Ala 195 200 205 Lys Arg His Arg Thr Gly Lys Ala Val Ala Ser Asp Asp Leu Gly Tyr 210 215 220 Ile Thr Phe Tyr Gly Asn Asp Ile Gly Ala Thr Ser Tyr Ala Ala Lys 225 230 235 240 Asp Ala Asp Asp Arg Glu Lys His Pro Ala Glu Tyr Thr Val Asp Phe 245 250 255 Asp Lys Lys Ile Leu Lys Gly Glu Leu Ile Lys Asn Gln Tyr Val Gln 260 265 270 Lys Lys Asn Asp Pro Lys Lys Pro Leu Thr Ile Tyr Asn Ile Thr Ala 275 280 285 Asp Leu Asn Gly Asn Arg Phe Thr Gly Ser Ala Lys Val Asn Thr Glu 290 295 300 Val Lys Thr Arg His Ala Asp Lys Glu Tyr Leu Phe Phe His Thr Asp 305 310 315 320 Ala Asp Gln Arg Leu Glu Gly Gly Phe Phe Gly Asp Asn Gly Glu Glu 325 330 335 Leu Ala Gly Arg Phe Ile Ser Asn Asp Asn Gly Val Phe Gly Val Phe 340 345 350 Ala Gly Lys Gln Lys Thr Asn Ala Ser Gly Thr Asn Pro Ala Met Pro 355 360 365 Phe Gly Lys His Thr Lys Ile Leu Asp Ser Leu Lys Ile Ser Val Asp 370 375 380 Glu Ala Thr Asp Glu Asn Pro Arg Pro Phe Glu Val Ser Thr Met Pro 385 390 395 400 Asp Phe Gly His Pro Asp Lys Leu Leu Val Glu Gly Arg Glu Ile Pro 405 410 415 Leu Val Ser Lys Glu Lys Thr Ile Asp Leu Ala Asp Gly Arg Lys Met 420 425 430 Thr Val Ser Ala Cys Cys Asp Phe Leu Thr Tyr Val Lys Leu Gly Arg 435 440 445 Ile Lys Thr Glu Arg Pro Ala Val Lys Pro Lys Ala Gln Asp Glu Glu 450 455 460 Asp Ser Gly Ile Asn Asn Gly Glu Glu Ser Glu Asp Glu Glu Glu Ile 465 470 475 480 Ala Glu Glu Ser Glu Asp Glu Val Ser Glu Asp Asp Asn Gly Glu Asp 485 490 495 Glu Asp Glu Ile Val Glu Glu Glu Ala Asp Glu Ala Glu Glu Ile Glu 500 505 510 Glu Glu Ala Glu Glu Glu Glu Pro Glu Glu Glu Ser Pro Glu Glu Gly 515 520 525 Asn Gly Val Ser Asp Gly Ile Pro Pro Ala Pro Glu Ala Leu Lys Gly 530 535 540 Arg Asp Ile Asp Leu Phe Leu Lys Gly Ile Arg Thr Ala Glu Ala Asp 545 550 555 560 Ile Pro Lys Thr Gly Thr Ala His Tyr Thr Gly Thr Trp Glu Ala Arg 565 570 575 Ile Gly Glu Pro Ile Gln Trp Asp Asn Lys Ala Asp Lys Ala Ala Lys 580 585 590 Ala Glu Phe Asp Val Asp Phe Gly Asn Lys Ser Ile Ser Gly Thr Leu 595 600 605 Thr Glu Gln Asn Gly Val Glu Pro Ala Phe Arg Ile Glu Asn Gly Val 610 615 620 Ile Glu Gly Asn Gly Phe His Pro Thr Ala Arg Thr Arg Asp Asn Gly 625 630 635 640 Ile Asn Leu Ser Gly Asn Gly Ser Thr Asn Pro Gln Ser Phe Lys Ala 645 650 655 Asp Asn Leu Leu Val Thr Gly Gly Phe Tyr Gly Pro Gln Ala Ala Glu 660 665 670 Leu Gly Gly Thr Ile Phe Asn Lys Asp Gly Lys Ser Leu Gly Ile Thr 675 680 685 Glu Asp Ile Glu Asn Glu Val Glu Asn Glu Ala Asp Val Gly Glu Gln 690 695 700 Leu Glu Pro Glu Val Lys Pro Gln Phe Gly Val Val Phe Gly Ala Lys 705 710 715 720 Lys Asp Asn Lys Glu Val Glu Lys 725 82 907 PRT Neisseria gonorrhoeae 82 Met Gln Gln Gln His Leu Phe Arg Phe Asn Ile Leu Cys Leu Ser Leu 1 5 10 15 Met Thr Ala Leu Pro Ala Tyr Ala Glu Asn Val Gln Ala Gly Gln Ala 20 25 30 Gln Glu Lys Gln Leu Asp Thr Ile Gln Val Lys Ala Lys Lys Gln Lys 35 40 45 Thr Arg Arg Asp Asn Glu Val Thr Gly Leu Gly Lys Leu Val Lys Thr 50 55 60 Ala Asp Thr Leu Ser Lys Glu Gln Val Leu Asp Ile Arg Asp Leu Thr 65 70 75 80 Arg Tyr Asp Pro Gly Ile Ala Val Val Glu Gln Gly Arg Gly Ala Ser 85 90 95 Ser Gly Tyr Ser Ile Arg Gly Met Asp Lys Asn Arg Val Ser Leu Thr 100 105 110 Val Asp Gly Leu Ala Gln Ile Gln Ser Tyr Thr Ala Gln Ala Ala Leu 115 120 125 Gly Gly Thr Arg Thr Ala Gly Ser Ser Gly Ala Ile Asn Glu Ile Glu 130 135 140 Tyr Glu Asn Val Lys Ala Val Glu Ile Ser Lys Gly Ser Asn Ser Val 145 150 155 160 Glu Gln Gly Ser Gly Ala Leu Ala Gly Ser Val Ala Phe Gln Thr Lys 165 170 175 Thr Ala Asp Asp Val Ile Gly Glu Gly Arg Gln Trp Gly Ile Gln Ser 180 185 190 Lys Thr Ala Tyr Ser Gly Lys Asn Arg Gly Leu Thr Gln Ser Ile Ala 195 200 205 Leu Ala Gly Arg Ile Gly Gly Ala Glu Ala Leu Leu Ile Arg Thr Gly 210 215 220 Arg His Ala Gly Glu Ile Arg Ala His Glu Ala Ala Gly Arg Gly Val 225 230 235 240 Gln Ser Phe Asn Arg Leu Ala Pro Val Glu Asp Gly Ser Asp Tyr Ala 245 250 255 Tyr Phe Val Val Glu Gly Glu Cys Pro Asp Gly Tyr Ala Ala Cys Lys 260 265 270 Asp Lys Pro Lys Lys Asp Val Val Gly Glu Asp Lys Arg Gln Thr Val 275 280 285 Ser Thr Arg Asp Tyr Thr Gly Pro Asn Arg Phe Leu Ala Asp Pro Leu 290 295 300 Ser Tyr Glu Ser Arg Ser Trp Leu Phe Arg Pro Gly Phe Arg Phe Glu 305 310 315 320 Asn Lys Arg His Tyr Ile Gly Gly Ile Leu Glu Arg Thr Gln Gln Thr 325 330 335 Phe Asp Thr Arg Asp Met Thr Val Pro Ala Phe Leu Thr Lys Ala Val 340 345 350 Phe Asp Ala Asn Ser Lys Gln Ala Gly Ser Leu Arg Gly Asn Gly Lys 355 360 365 Tyr Ala Gly Asn His Lys Tyr Gly Gly Leu Phe Thr Asn Gly Glu Asn 370 375 380 Asn Ala Pro Val Gly Ala Glu Tyr Gly Thr Gly Val Phe Tyr Asp Glu 385 390 395 400 Thr His Thr Lys Ser Arg Tyr Gly Leu Glu Tyr Val Tyr Thr Asn Ala 405 410 415 Asp Lys Asp Thr Trp Ala Asp Tyr Ala Arg Leu Ser Tyr Asp Arg Gln 420 425 430 Gly Ile Gly Leu Asp Asn His Phe Gln Gln Thr His Cys Ser Ala Asp 435 440 445 Gly Ser Asp Lys Tyr Cys Arg Pro Ser Ala Asp Lys Pro Phe Ser Tyr 450 455 460 Tyr Lys Ser Asp Arg Val Ile Tyr Gly Glu Ser His Arg Leu Leu Gln 465 470 475 480 Ala Ala Phe Lys Lys Ser Phe Asp Thr Ala Lys Ile Arg His Asn Leu 485 490 495 Ser Val Asn Leu Gly Tyr Asp Arg Phe Gly Ser Asn Leu Arg His Gln 500 505 510 Asp Tyr Tyr Tyr Gln Ser Ala Asn Arg Ala Tyr Ser Leu Lys Thr Pro 515 520 525 Pro Gln Asn Asn Gly Lys Lys Thr Ser Pro Tyr Trp Val Ser Ile Gly 530 535 540 Arg Gly Asn Val Val Thr Gly Gln Ile Cys Arg Ser Gly Asn Asn Thr 545 550 555 560 Tyr Thr Asp Cys Thr Pro Arg Ser Ile Asn Gly Lys Ser Tyr Tyr Ala 565 570 575 Ala Val Arg Asp Asn Val Arg Leu Gly Arg Trp Ala Asp Val Gly Ala 580 585 590 Gly Leu Arg Tyr Asp Tyr Arg Ser Thr His Ser Asp Asp Gly Ser Val 595 600 605 Ser Thr Gly Thr His Arg Thr Leu Ser Trp Asn Thr Gly Ile Val Leu 610 615 620 Lys Pro Ala Asp Trp Leu Asp Leu Thr Tyr Arg Thr Ser Thr Gly Phe 625 630 635 640 Arg Leu Pro Ser Phe Ala Glu Met Tyr Gly Trp Arg Ser Gly Asp Lys 645 650 655 Ile Lys Ala Val Lys Ile Asp Pro Glu Lys Ser Phe Asn Lys Glu Ala 660 665 670 Gly Ile Val Phe Lys Gly Asp Phe Gly Asn Leu Glu Ala Ser Trp Phe 675 680 685 Asp Asn Ala Tyr Arg Asp Leu Ile Val Arg Gly Tyr Glu Ala Glu Ile 690 695 700 Lys Asn Gly Lys Glu Gln Ala Lys Gly Ala Pro Ala Tyr Leu Asn Ala 705 710 715 720 Gln Ser Ala Arg Ile Thr Gly Ile Asn Ile Leu Gly Lys Ile Asp Trp 725 730 735 Asn Gly Val Trp Asp Lys Leu Pro Glu Gly Trp Tyr Ser Thr Phe Ala 740 745 750 Tyr Asn Arg Val Arg Val Arg Asp Ile Lys Lys Arg Ala Asp Arg Thr 755 760 765 Asp Ile Gln Ser His Leu Phe Asp Ala Ile Gln Pro Ser Arg Tyr Val 770 775 780 Val Gly Ser Gly Tyr Asp Gln Pro Glu Gly Lys Trp Gly Val Asn Gly 785 790 795 800 Met Leu Thr Tyr Ser Lys Ala Lys Glu Ile Thr Glu Leu Leu Gly Ser 805 810 815 Arg Ala Leu Leu Asn Gly Asn Ser Arg Asn Thr Lys Ala Thr Ala Arg 820 825 830 Arg Thr Arg Pro Trp Tyr Ile Val Asp Val Ser Gly Tyr Tyr Thr Val 835 840 845 Lys Lys His Phe Thr Leu Arg Ala Gly Val Tyr Asn Leu Leu Asn His 850 855 860 Arg Tyr Val Thr Trp Glu Asn Val Arg Gln Thr Ala Ala Gly Ala Val 865 870 875 880 Asn Gln His Lys Asn Val Gly Val Tyr Asn Arg Tyr Ala Ala Pro Gly 885 890 895 Arg Asn Tyr Thr Phe Ser Leu Glu Met Lys Phe 900 905 83 3035 DNA Neisseria gonorrhoeae 83 cgaagagttg ggcggatggt ttgcctatcc gggcaatgaa caaacgaaaa atgcgcaagc 60 ttcatccggc aatggaaatt cagcaggcag cgcgaccgtg gtattcggtg cgaaacgcca 120 aaagcttgtg caataagcac ggctgccgaa caatcgagaa taaggcttca gacggcatcg 180 ttcctgccga ttccgtctga aagcgaagat tagggaaaca ctatgcaaca gcaacatttg 240 ttccgattca atattttatg cctgtcttta atgactgcgc tgcccgctta tgcagaaaat 300 gtgcaagccg gacaagcaca ggaaaaacag ttggacacca tacaggtaaa agccaaaaaa 360 cagaaaaccc gccgcgataa cgaagtaacc ggtttgggca aattggtcaa aaccgccgac 420 acactcagca aagaacaggt actcgacatc cgcgacctga cgcgttacga ccccggcatc 480 gccgtcgtcg aacaggggcg cggcgcaagc tcgggctact cgatacgcgg tatggacaaa 540 aaccgcgtct ccttgacggt ggacggcttg gcgcaaatac agtcctacac cgcgcaggcg 600 gcattgggcg ggacgaggac ggcgggcagc agcggcgcaa tcaatgaaat cgagtatgaa 660 aacgttaagg ctgtcgaaat cagcaaaggc tcaaactcgg tcgaacaagg cagcggcgca 720 ttggcgggtt cggtcgcatt tcaaaccaaa accgcagacg atgttatcgg ggaaggcagg 780 cagtggggca ttcagagtaa aaccgcctat tccggcaaaa accgggggct tacccaatcc 840 atcgcgctgg cggggcgcat cggcggtgcg gaggctttgc tgatccgcac cggccggcac 900 gcgggggaaa tccgcgccca cgaagccgcc ggacgcggcg ttcagagctt taacaggctg 960 gcgccggttg aagacggcag tgactatgcc tattttgtgg tcgaaggaga atgccctgat 1020 ggatatgcgg cttgtaaaga caaaccgaaa aaagatgttg tcggcgaaga caaacgtcaa 1080 acggtttcca cccgagacta cacgggcccc aaccgcttcc ttgccgatcc gctttcatac 1140 gaaagccggt cgtggctgtt ccgcccgggt tttcgttttg agaataagcg gcactacatc 1200 ggcggcatac tcgaacgcac gcaacaaact ttcgacacgc gcgatatgac ggttccggca 1260 ttcctgacca aggcggtttt tgatgcaaat tcaaaacagg cgggttcttt gcgcggcaac 1320 ggcaaatacg cgggcaacca caaatacggc gggctgttta ccaacggcga aaacaatgcg 1380 ccggtgggcg cggaatacgg tacgggcgtg ttttacgacg agacgcacac caaaagccgc 1440 tacggtttgg aatatgtcta taccaatgcc gataaagaca cttgggcgga ttatgcccgc 1500 ctctcttacg accggcaggg catcggtttg gacaaccatt ttcagcagac gcactgttct 1560 gccgacggtt cggacaaata ttgccgcccg agtgccgaca agccgttttc ctattacaaa 1620 tccgaccgcg tgatttacgg ggaaagccac aggctcttgc aggcggcatt caaaaaatcc 1680 ttcgataccg ccaaaatccg ccacaacctg agcgtgaatc tcggttacga ccgcttcggc 1740 tctaatctgc gccatcagga ttattattat caaagtgcca accgcgccta ttcgttgaaa 1800 acgccccctc aaaacaacgg caaaaaaacc agcccctatt gggtcagcat aggcagggga 1860 aatgtcgtta cggggcaaat ctgccgctcg ggcaacaata cttatacgga ctgcacgccg 1920 cgcagcatca acggcaaaag ctattacgcg gcggtccggg acaatgtccg tttgggcagg 1980 tgggcggatg tcggcgcggg cttgcgctac gactaccgca gcacgcattc ggacgacggc 2040 agcgtttcca ccggcacgca ccgcaccctg tcctggaaca ccggcatcgt cctcaaacct 2100 gccgactggc tggatttgac ttaccgcact tcaaccggct tccgcctgcc ctcgtttgcg 2160 gaaatgtacg gctggcggtc gggcgataaa ataaaagccg tcaaaatcga tccggaaaaa 2220 tcgttcaaca aagaagccgg catcgtgttt aaaggcgatt tcggcaactt ggaggcaagt 2280 tggttcgaca atgcctaccg cgatttgatt gtccggggtt atgaagcgga aattaaaaac 2340 ggcaaagaac aagccaaagg cgccccggct tacctcaatg cccaaagcgc gcggattacc 2400 ggcatcaata ttttgggcaa aatcgattgg aacggcgtat gggataaatt gcccgaaggt 2460 tggtattcta catttgccta taatcgtgtc cgtgtccgcg acatcaaaaa acgcgcagac 2520 cgcaccgata ttcaatcaca cctgtttgat gccatccaac cctcgcgcta tgtcgtcggc 2580 tcgggctatg accaaccgga aggcaaatgg ggcgtgaacg gtatgctgac ttattccaaa 2640 gccaaggaaa tcacagagtt gttgggcagc cgggctttgc tcaacggcaa cagccgcaat 2700 acaaaagcca ccgcgcgccg tacccgccct tggtatattg tggatgtgtc cggttattac 2760 acggttaaaa aacacttcac cctccgtgcg ggcgtgtaca acctcctcaa ccaccgctat 2820 gttacttggg aaaatgtgcg gcaaactgcc gccggcgcag tcaaccaaca caaaaatgtc 2880 ggcgtttaca accgatatgc cgcccccggc cgcaactaca catttagctt ggaaatgaag 2940 ttctaaacgt ccgaacgccg caaatgccgt ctgaaaggct tcagacggcg ttttttacac 3000 aatccccacc gtttcccatc cttcccgata caccg 3035 84 683 PRT Neisseria gonorrhoeae 84 Met Asn Asn Pro Leu Val Asn Gln Ala Ala Met Val Leu Pro Val Phe 1 5 10 15 Leu Leu Ser Ala Cys Leu Gly Gly Gly Gly Ser Phe Asp Leu Asp Ser 20 25 30 Val Asp Thr Glu Ala Pro Arg Ala Ala Pro Lys Tyr Gln Asp Val Pro 35 40 45 Ser Lys Lys Pro Glu Ala Arg Lys Asp Gln Gly Gly Tyr Gly Phe Ala 50 55 60 Met Arg Phe Lys Arg Arg Asn Trp Tyr Arg Ala Ala Asn Glu Asn Glu 65 70 75 80 Val Lys Leu Lys Glu Ser Asp Trp Glu Gln Thr Asp Asp Asp Glu Ile 85 90 95 Lys Asn Pro Phe Lys Gln Lys Asn Ile Ile Asn Ala Leu Pro Gly Asn 100 105 110 Glu Gly Glu Leu Leu Gln Asp Ser Ser Gln Gln Gly Lys Gly Thr Ser 115 120 125 Lys Val Arg Asp His His Asp Phe Lys Tyr Val Trp Ser Gly Phe Phe 130 135 140 Tyr Lys Arg Ile Lys Ile Thr Thr Lys Lys Asp Glu Ser His Lys Ile 145 150 155 160 Ile Glu Ala Arg Ser Gly Pro Asp Gly Tyr Ile Phe Tyr Lys Gly Arg 165 170 175 Asn Pro Ser Arg Lys Leu Pro Val Ser Gly Glu Val Thr Tyr Lys Gly 180 185 190 Thr Trp Asp Phe Leu Thr Asp Val Lys Ala Asn Gln Lys Phe Thr Asp 195 200 205 Leu Gly Asn Ala Ser Thr Lys Ser Gly Asp Gln Tyr Ser Ala Phe Ser 210 215 220 Gly Glu Leu Asp Tyr Ile Val Lys Lys Glu Glu Asp Lys Lys Glu Lys 225 230 235 240 His Lys Gly Leu Gly Leu Thr Thr Glu Ile Thr Val Asp Phe Glu Lys 245 250 255 Lys Thr Leu Ile Gly Lys Leu Ile Lys Asn Asn Met Leu Ile Asn Asn 260 265 270 Asn Thr Lys Pro Thr Thr Gln Tyr Tyr Ser Leu Glu Ala Gln Val Thr 275 280 285 Gly Asn Arg Phe Ser Gly Lys Ala Met Ala Thr Glu Lys Gly Glu Asn 290 295 300 Lys Gln His Pro Phe Val Ser Asp Ser Ser Ser Leu Ser Gly Gly Phe 305 310 315 320 Phe Gly Pro Gln Gly Glu Glu Leu Gly Phe Arg Phe Leu Ser Asp Asp 325 330 335 Gly Lys Val Ala Val Val Gly Ser Ala Lys Thr Lys Asp Glu Thr Ala 340 345 350 Ser Ser Gly Gly Thr Ser Gly Gly Ala Ser Val Ser Thr Ser Asn Gly 355 360 365 Ala Ala Gly Thr Ser Ser Glu Asn Lys Leu Thr Thr Val Leu Asp Ala 370 375 380 Val Glu Leu Thr Pro Asn Gly Lys Lys Ile Lys Asp Leu Asp Asn Phe 385 390 395 400 Ser Asn Ala Ala Gln Leu Val Val Asp Gly Ile Met Ile Pro Leu Leu 405 410 415 Pro Lys Asp Ser Glu Ser Gly Gly Ser His Thr Asp Lys Gly Glu Asn 420 425 430 Gly Lys Thr Ala Phe Ile Tyr Glu Thr Thr Tyr Thr Pro Glu Ser Asp 435 440 445 Lys Glu Asp Ala Gln Thr Gly Met Ala Thr Asn Gly Val Gln Thr Val 450 455 460 Ser Asn Thr Ala Gly Gly Thr Ser Gly Lys Thr Lys Thr His Tyr Glu 465 470 475 480 Val Gln Ala Cys Cys Ser Asn Leu Asn Tyr Leu Lys Tyr Gly Leu Leu 485 490 495 Thr Arg Lys Asn Ser Ser Gln Ala Asp Ala Lys Met Gly Gln Val Glu 500 505 510 Gln Ser Met Phe Leu Gln Gly Glu Arg Thr Asp Glu Lys Glu Ile Pro 515 520 525 Gln Glu Gln Asn Val Val Tyr Ser Gly Thr Trp Tyr Gly His Ile Ala 530 535 540 Thr Asn Gly Thr Ser Trp Thr Arg Glu Ala Ser Asp Gln Glu Asn Gly 545 550 555 560 Asn Arg Ala Asn Phe Asp Val Asn Phe Lys Asp Lys Arg Ile Thr Gly 565 570 575 Thr Leu Thr Ala Glu Asn Arg Ser Glu Ala Thr Phe Thr Ile Glu Ala 580 585 590 Met Ile Glu Gly Asn Gly Phe Lys Gly Thr Ala Lys Thr Gly Asn Gly 595 600 605 Gly Phe Ala Pro Asp Gln Asn Ser Ser Thr Gly Thr His Lys Val His 610 615 620 Ile Thr Asn Ala Ala Val Gln Gly Gly Phe Tyr Gly Pro Asn Ala Glu 625 630 635 640 Glu Leu Gly Gly Trp Phe Ala Tyr Pro Gly Asn Gly Gln Thr Lys Asn 645 650 655 Ala Gln Thr Ser Ser Gly Asn Gly Asn Ser Ala Gly Ser Ala Thr Val 660 665 670 Val Phe Gly Ala Lys Arg Gln Gln Leu Val Lys 675 680 85 2052 DNA Neisseria gonorrhoeae 85 atgaacaatc cattggtgaa tcaggctgct atggtgctgc ccgtgttttt gttgagcgct 60 tgtctgggcg gaggcggcag tttcgatctt gattctgtcg ataccgaagc cccgcgtgcc 120 gcgccaaagt atcaagatgt tccttccaaa aaaccggaag cccgaaaaga ccaaggcgga 180 tacggtttcg cgatgcgctt caagcggcgg aattggtatc gggcggcaaa cgaaaacgag 240 gttaaactga aagagagtga ttgggaacaa acggatgatg atgagatcaa aaaccctttc 300 aaacaaaaaa atattattaa tgccttacct ggaaatgagg gggaattatt gcaagattcc 360 agtcaacaag gtaagggtac atctaaggtt agggaccatc acgattttaa atacgtatgg 420 tcgggttttt tttataaacg gattaagatt acaactaaaa aagacgaatc tcataaaata 480 atcgaagcca gaagcggtcc tgacggttat attttttata aaggcagaaa tccctcgaga 540 aaacttcctg tttcagggga ggttacgtac aaaggtactt gggatttttt aactgatgtg 600 aaagcaaatc agaaatttac agatttagga aatgcttcta cgaaatccgg agaccaatat 660 agtgcttttt ccggggagtt ggattatata gtcaaaaaag aggaggataa aaaagaaaag 720 cacaaaggtt tgggattaac aacggaaata acggttgatt ttgagaaaaa aaccctgatc 780 ggaaaattaa ttaaaaacaa catgttaatc aataataaca ctaaacccac cacccaatat 840 tacagccttg aggctcaagt aacaggcaac cgcttcagcg gcaaggcgat ggcaaccgaa 900 aaaggcgaaa acaaacaaca tccctttgtt tccgactcgt cttctctgag cggcggcttt 960 ttcggcccgc agggtgagga attgggtttc cgctttttga gcgacgatgg aaaagttgcc 1020 gttgtcggca gcgcgaaaac caaagacgaa accgcaagca gtggcggcac ttcgggcggt 1080 gcaagcgttt ccacatcaaa cggtgcggca ggcacgtcgt ctgaaaacaa gctgaccacg 1140 gttttggatg cggttgaatt gacaccaaac ggcaagaaaa tcaaagatct cgacaacttc 1200 agcaacgccg cccaactggt tgtcgacggc attatgattc cgctcctgcc caaggattcc 1260 gaaagcgggg gcagtcatac agataaaggt gaaaacggca aaacagcctt tatctacgaa 1320 acaacctaca cgccggaaag tgataaagaa gacgctcaaa caggtatggc gaccaatggc 1380 gtgcaaaccg tttcaaatac ggcaggcggc acaagtggca aaacaaaaac ccattatgaa 1440 gtccaagcct gctgttccaa cctcaattat ctgaaatacg ggttgctgac gcgtaaaaac 1500 agtagtcaag ctgacgctaa aatgggacaa gttgaacaaa gtatgttcct ccaaggcgag 1560 cgcaccgatg aaaaagaaat tccacaagaa caaaatgtcg tttattcagg cacttggtac 1620 gggcatattg ccaccaacgg cacaagttgg acccgcgaag cctccgatca ggaaaatggt 1680 aatcgggcaa attttgacgt gaatttcaaa gacaaaagaa ttaccggcac gttaaccgct 1740 gaaaacaggt cggaggcaac ctttaccatt gaagccatga ttgagggcaa cggctttaaa 1800 ggtacggcga aaaccggtaa tggcggcttt gcgccggatc aaaacagcag caccggtaca 1860 cataaagtgc acatcacaaa tgccgcggtg cagggcggtt tttacgggcc taacgccgaa 1920 gagttgggcg gttggtttgc ctatccgggc aatggacaaa cgaaaaatgc gcaaacttca 1980 tccggcaatg gaaattcagc aggcagcgcg accgtggtat tcggtgcgaa acgccaacag 2040 cttgtgaaat aa 2052 86 215 PRT Neisseria gonorrhoeae 86 Met Ala Ser Lys Ser Ser Lys Thr Asn Leu Asp Leu Asn Asn Leu His 1 5 10 15 Leu Leu Asn Leu Pro Ala Arg Leu Phe Ile Ala Leu Leu Val Val Ala 20 25 30 Ala Val Leu Gly Leu Gly Tyr Ala Gly Leu Phe Lys Ser Gln Met Glu 35 40 45 Ser Leu Glu Glu Tyr Glu Ala Lys Glu Thr Glu Leu Lys Asn Thr Tyr 50 55 60 Lys Gln Lys Ser Ile Asp Ala Ala Ser Leu Asn Asn Leu Arg Asp Glu 65 70 75 80 Leu Ala Ser Ile Arg Ser Ala Phe Asp Ile Met Leu Lys Gln Leu Pro 85 90 95 Thr Asp Ala Glu Ile Pro Asn Leu Val Gln Glu Leu His Gln Ala Gly 100 105 110 Ser Ser Asn Gly Leu Arg Leu Asp Ser Val Met Pro Gln Pro Pro Val 115 120 125 Asp Asp Gly Pro Ile Lys Lys Leu Pro Tyr Ser Ile Ser Ile Thr Gly 130 135 140 Asn Tyr Glu Gln Ile Ser Gln Phe Thr Arg Asp Val Gly Ser Leu Ser 145 150 155 160 Arg Ile Ile Thr Leu Glu Ser Leu Lys Ile Ala Gln Ser Pro Glu Asn 165 170 175 Gly Gly Asn Pro Asp Gly Lys Ser Ser Ile Leu Asn Leu Ser Ala Ile 180 185 190 Ala Thr Thr Tyr Gln Ala Lys Ser Ile Glu Glu Leu Ala Ala Glu Ala 195 200 205 Ala Gln Asn Ala Glu Gln Lys 210 215 87 180 PRT Neisseria gonorrhoeae 87 Met Lys His Tyr Ala Leu Leu Ile Ser Phe Leu Ala Leu Ser Ala Cys 1 5 10 15 Ser Gln Ser Ser Glu Asp Leu Asn Glu Trp Met Ala Gln Thr Arg Arg 20 25 30 Glu Ala Lys Ala Glu Ile Ile Pro Phe Gln Ala Pro Thr Leu Pro Val 35 40 45 Ala Pro Val Tyr Ser Pro Pro Gln Leu Thr Gly Pro Asn Ala Phe Asp 50 55 60 Phe Arg Arg Met Glu Thr Ala Lys Lys Gly Glu Asn Ala Pro Asp Thr 65 70 75 80 Lys Arg Ile Lys Glu Thr Leu Glu Lys Phe Ser Leu Glu Asn Met Arg 85 90 95 Tyr Val Gly Ile Leu Lys Ser Gly Gln Lys Val Ser Gly Phe Ile Glu 100 105 110 Ala Glu Gly Tyr Val Tyr Thr Val Gly Val Gly Asn Tyr Leu Gly Gln 115 120 125 Asn Tyr Gly Arg Ile Glu Ser Ile Thr Asp Asp Ser Ile Ile Leu Asn 130 135 140 Glu Leu Ile Glu Asp Ser Thr Gly Asn Trp Val Ser Arg Lys Ala Glu 145 150 155 160 Leu Leu Leu Asn Ser Ser Asp Lys Asn Thr Glu Gln Ala Ala Gln Pro 165 170 175 Glu Glu Gln Asn 180 88 720 PRT Neisseria gonorrhoeae 88 Met Asn Thr Lys Leu Thr Lys Ile Ile Ser Gly Leu Phe Val Ala Thr 1 5 10 15 Ala Ala Phe Gln Thr Ala Ser Ala Gly Asn Ile Thr Asp Ile Lys Val 20 25 30 Ser Ser Leu Pro Asn Lys Gln Lys Ile Val Lys Val Ser Phe Asp Lys 35 40 45 Glu Ile Val Asn Pro Thr Gly Phe Val Thr Ser Ser Pro Ala Arg Ile 50 55 60 Ala Leu Asp Phe Glu Gln Thr Gly Ile Ser Met Asp Gln Gln Val Leu 65 70 75 80 Glu Tyr Ala Asp Pro Leu Leu Ser Lys Ile Ser Ala Ala Gln Asn Ser 85 90 95 Ser Arg Ala Arg Leu Val Leu Asn Leu Asn Lys Pro Gly Gln Tyr Asn 100 105 110 Thr Glu Val Arg Gly Asn Lys Val Trp Ile Phe Ile Asn Glu Ser Asp 115 120 125 Asp Thr Val Ser Ala Pro Ala Arg Pro Ala Val Lys Ala Ala Pro Ala 130 135 140 Ala Pro Ala Lys Gln Gln Ala Ala Ala Pro Phe Thr Glu Ser Val Val 145 150 155 160 Ser Val Ser Ala Pro Phe Ser Pro Ala Lys Gln Gln Ala Ala Ala Ser 165 170 175 Ala Lys Gln Gln Ala Ala Thr Pro Ala Lys Gln Thr Asn Ile Asp Phe 180 185 190 Arg Lys Asp Gly Lys Asn Ala Gly Ile Ile Glu Leu Ala Ala Leu Gly 195 200 205 Phe Ala Gly Gln Pro Asp Ile Ser Gln Gln His Asp His Ile Ile Val 210 215 220 Thr Leu Lys Asn His Thr Leu Pro Thr Ala Leu Gln Arg Ser Leu Asp 225 230 235 240 Val Ala Asp Phe Lys Thr Pro Val Gln Lys Val Thr Leu Lys Arg Leu 245 250 255 Asn Asn Asp Thr Gln Leu Ile Ile Thr Thr Thr Gly Asn Trp Glu Leu 260 265 270 Val Asn Lys Ser Ala Ala Pro Gly Tyr Phe Thr Phe Gln Val Leu Pro 275 280 285 Lys Lys Gln Asn Leu Glu Ser Gly Gly Val Asn Asn Ala Pro Lys Thr 290 295 300 Phe Thr Gly Arg Lys Ile Ser Leu Asp Phe Gln Asp Val Glu Ile Arg 305 310 315 320 Thr Ile Leu Gln Ile Leu Ala Lys Glu Ser Gly Met Asn Ile Val Ala 325 330 335 Ser Asp Ser Val Ser Gly Lys Met Thr Leu Ser Leu Lys Asp Val Pro 340 345 350 Trp Asp Gln Ala Leu Asp Leu Val Met Gln Ala Arg Asn Leu Asp Met 355 360 365 Arg Gln Gln Gly Asn Ile Val Asn Met Ala Pro Arg Arg Ala Ala Cys 370 375 380 Gln Arg Gln Ser Leu Leu Thr Ser Gly Lys Arg His Cys Arg Ser Gly 385 390 395 400 Arg Ala Val Phe Pro Lys Leu Pro Ile Glu Ile Gln Lys Cys Gly Arg 405 410 415 Ile Pro Gln His Pro Ala Leu Asp Asn Ala Asp Thr Thr Gly Asn Arg 420 425 430 Asn Thr Leu Val Ser Gly Arg Gly Ser Val Leu Ile Asp Pro Ala Thr 435 440 445 Asn Thr Leu Ile Val Thr Asp Thr Arg Ser Val Ile Glu Lys Phe Arg 450 455 460 Lys Leu Ile Asp Glu Leu Asp Val Pro Ala Gln Gln Val Met Ile Glu 465 470 475 480 Ala Arg Ile Val Glu Ala Ala Asp Gly Phe Ser Arg Asp Leu Gly Val 485 490 495 Lys Phe Gly Ala Thr Gly Arg Lys Lys Leu Lys Asn Glu Thr Ser Ala 500 505 510 Phe Gly Trp Gly Val Asn Ser Gly Phe Gly Gly Gly Asp Lys Trp Glu 515 520 525 Ala Lys Pro Lys Ser Thr Cys Arg Leu Pro Cys Arg Lys Gln His Phe 530 535 540 Ala Gly Ala Arg Asp Phe Ser Gly Ala Leu Asn Leu Glu Leu Ser Ala 545 550 555 560 Ser Glu Ser Leu Ser Lys Thr Lys Thr Leu Ala Asn Pro Arg Val Leu 565 570 575 Thr Gln Asn Arg Lys Glu Ala Lys Ile Glu Ser Gly Tyr Glu Ile Pro 580 585 590 Phe Thr Val Thr Thr Arg Ser Gly Gly Gly Asn Ser Thr Asn Thr Glu 595 600 605 Leu Lys Lys Ala Val Leu Gly Leu Thr Val Thr Ala Asn Ile Thr Pro 610 615 620 Asp Gly Gln Ile Ile Met Thr Val Lys Ile Asn Lys Asp Ser Pro Arg 625 630 635 640 Gln Cys Ala Ser Gly Asn Asn Thr Ile Leu Cys Ile Ser Thr Lys Ser 645 650 655 Leu Asn Thr Gln Ala Met Val Glu Asn Gly Gly Thr Leu Ile Val Gly 660 665 670 Gly Ile Tyr Glu Glu Asn Asn Gly Asn Thr Leu Thr Lys Val Pro Leu 675 680 685 Leu Ala Thr Ser Pro Leu Ser Ala Thr Ser Leu Lys His Ser Gly Lys 690 695 700 Asn Arg Pro Pro Arg Thr Ala Asp Phe Gln Leu Pro Pro Arg Glu Leu 705 710 715 720 89 3829 DNA Neisseria gonorrhoeae 89 aaacgcacag gaggaaactg aatggcttct aaatcatcta aaaccaactt ggatctcaac 60 aaccttcacc tgctcaacct tcctgccagg ctttttatcg ccctgctggt cgttgccgcc 120 gtgctggggc tcggttatgc cggattgttc aaaagccaga tggaatccct tgaggaatat 180 gaagcaaaag aaaccgaact gaaaaacacc tacaaacaga aaagtatcga cgcggccagc 240 ctgaacaacc ttagggacga acttgcctca atccgctctg ccttcgatat catgttgaaa 300 cagctgccga cagatgcaga aattcccaat ttggttcaag agcttcatca ggcgggttcg 360 agcaacggtc tgcgcttgga cagcgttatg ccccaacctc ccgtagatga cggtcccatc 420 aaaaaattac cctattccat ttccattacc ggaaattacg aacagatcag ccaatttacc 480 cgcgatgtcg gcagtctctc ccgaatcatt acccttgagt cgctgaaaat cgcccaatct 540 ccggaaaacg gcggcaatcc tgacggcaag agcagtatcc tgaacctcag cgccattgcc 600 accacctacc aagcaaaatc catagaagag cttgccgcag aagcggcaca aaatgccgag 660 caaaaataac ttacgttagg gaaaccatga aacactatgc cttactcatc agctttctgg 720 ctctctccgc gtgttcccaa agttctgaag acctaaacga atggatggca caaacgcgac 780 gcgaagccaa agcagaaatc atacctttcc aagcacctac cctgccggtt gcgccggtat 840 acagcccgcc gcagcttaca gggccgaacg cattcgactt ccgccgcatg gaaaccgcca 900 aaaaagggga aaatgccccc gacaccaagc gtattaaaga aacgctggaa aaattcagtt 960 tggaaaatat gcgttatgtc ggcattttga agtccggaca gaaagtctcc ggcttcatcg 1020 aggctgaagg ttatgtctac actgtcggtg tcggcaacta tttgggacaa aactacggta 1080 gaatcgaaag cattaccgac gacagcatca tcctgaacga gctgatagaa gacagcacgg 1140 gcaactgggt ttcccgtaaa gcagaactgc tgttgaattc ttccgacaaa aacaccgaac 1200 aagcggcaca gcctgaggaa caaaattaag aagaggatta ctccattatg aataccaaac 1260 tgacaaaaat catttccggt ctctttgtcg caaccgccgc ctttcagacg gcatcggcag 1320 gaaacattac agacatcaaa gtttcctccc tgcccaacaa acagaaaatc gtcaaagtca 1380 gctttgacaa agagattgtc aacccgaccg gcttcgtaac ctcctcaccg gcccgcatcg 1440 ccttggactt tgaacaaacc ggcatttcca tggatcaaca ggtactcgaa tatgccgatc 1500 ctctgttgag caaaatcagt gccgcacaaa acagcagccg tgcgcgtctg gttctgaatt 1560 tgaacaaacc gggccaatac aataccgaag tacgcgggaa caaagtttgg atattcatta 1620 acgaatcgga cgataccgtg tccgcccccg cccgcccagc cgtaaaagcc gcgcctgccg 1680 caccggcaaa acaacaggct gccgcaccgt ttaccgagtc cgtagtatcc gtatccgcac 1740 cgttcagccc ggcaaaacaa caggcagcgg catcggcaaa acaacaggcg gcgacaccgg 1800 caaaacaaac caatatcgat ttccgcaaag acggcaaaaa tgccggcatt atcgaattgg 1860 cggcattggg ctttgccggg cagcccgaca tcagccaaca gcacgaccac atcatcgtta 1920 cgctgaaaaa ccataccctg ccgaccgcgc tccaacgcag tttggatgtg gcagacttca 1980 aaacaccggt tcaaaaggtt acgctgaaac gcctcaataa cgacacccag ctgattatca 2040 caacaaccgg caactgggaa ctcgtcaaca aatccgccgc gcccggatac tttaccttcc 2100 aagtcctgcc gaaaaaacaa aacctcgagt caggcggcgt gaacaatgcg cccaaaacct 2160 tcacaggccg gaaaatctcc cttgacttcc aagatgtcga aatccgcacc atcctgcaga 2220 ttttggcaaa agaatccggg atgaacattg ttgccagcga ctccgtcagc ggcaaaatga 2280 ccctctccct caaagacgta ccttgggatc aggctttgga tttggttatg caggcgcgca 2340 acctcgatat gcgccagcaa gggaacatcg tcaacatggc cccgcgacga gctgcttgcc 2400 aaagacaaag ccttcttaca agcggaaaaa gacattgccg atctgggcgc gctgtattcc 2460 caaaacttcc aattgaaata caaaaatgtg gaagaattcc gcagcatcct gctttggaca 2520 atgccgacac gaccggaaac cgcaacacgc ttgtcagcgg caggggcagc gtgctgatcg 2580 atcccgccac caacaccctg attgttaccg atacccgcag cgtcatcgaa aaattccgca 2640 aactgattga cgaattggac gtacccgcgc aacaagtgat gattgaggcg cgtatcgtcg 2700 aagcggcaga cggcttctcg cgcgatttgg gcgttaagtt cggcgcgaca ggcaggaaaa 2760 aactgaaaaa tgagacgagc gcattcggct ggggcgtgaa ctccggcttc gggggcggcg 2820 ataaatggga ggccaaacca aaatcaacct gccggttgcc gtgccgcaaa cagcatttcg 2880 ctggtgcgcg cgatttctcc ggcgcgttga atttggaatt gtccgcatcc gagtcgcttt 2940 caaaaaccaa aacgcttgcc aatccgcgcg tgctgaccca aaaccgcaaa gaggccaaaa 3000 tcgaatccgg ttacgaaatt ccttttaccg taactacacg ctcgggcggc ggcaactcta 3060 ccaacacgga actcaaaaaa gccgtcttgg ggctgaccgt tacggcgaac atcacgcccg 3120 acggacaaat catcatgacc gtcaaaatca acaaagactc gcctcgacaa tgtgcttcag 3180 gcaacaacac aatcctatgt atttcgacca aaagcctgaa tacgcaggct atggttgaaa 3240 acggcggcac tttgattgtc ggcggtattt atgaagaaaa caacggcaat acgctgacca 3300 aagtccccct gttggctaca tccccgttat cggcaacctc tttaaaacac tcgggaaaaa 3360 accgaccgcc gcgaactgct gattttcaat tacccccgag ggaattatag atacggcgca 3420 acagcctgcg ctattgatgc gtcaaaataa gggcatatgt tttacagcat atgccctttc 3480 tttatgcttt ttgccgcgac cgaaatgccg tcattcccgc gagcgaatcc aacttgtccg 3540 gtttcggttg tttttcgtct cgtaactttt gagccgtcat tcccgcgaaa tcggaaatcc 3600 agtccgttca gtttcggtca tttccgataa attcctgttg cttttcattt ctagattccc 3660 actttcgtgg aataacggcg gaagggataa atcctcgcaa tccaaagcct gctcatttcc 3720 acaaaaaaca gcaacccgaa acaccccgtc attcccgagc aggcggaatc tagaaccgca 3780 acgccaggaa tctgtcggat acggctgaaa ccgaacgact ggattcccg 3829 90 174 PRT Neisseria gonorrhoeae 90 Met Lys Lys Ala Leu Ala Ala Leu Ile Ala Leu Ala Leu Pro Ala Ala 1 5 10 15 Ala Leu Ala Glu Gly Ala Ser Gly Phe Tyr Val Gln Ala Asp Ala Ala 20 25 30 His Ala Lys Ala Ser Ser Ser Leu Gly Ser Ala Lys Gly Phe Ser Pro 35 40 45 Arg Ile Ser Ala Gly Tyr Arg Ile Asn Asp Leu Arg Phe Ala Val Asp 50 55 60 Tyr Thr Arg Tyr Lys Asn Tyr Lys Ala Pro Ser Thr Asp Phe Lys Leu 65 70 75 80 Tyr Ser Ile Gly Ala Ser Val Ile Tyr Asp Phe Asp Thr Gln Ser Pro 85 90 95 Val Lys Pro Tyr Phe Gly Ala Arg Leu Ser Leu Asn Arg Ala Ser Ala 100 105 110 His Leu Gly Gly Ser Asp Ser Phe Ser Lys Thr Ser Ala Gly Leu Gly 115 120 125 Val Leu Ala Gly Val Ser Tyr Ala Val Thr Pro Asn Val Asp Leu Asp 130 135 140 Ala Gly Tyr Arg Tyr Asn Tyr Val Gly Lys Val Asn Thr Val Lys Asn 145 150 155 160 Val Arg Ser Gly Glu Leu Ser Ala Gly Val Arg Val Lys Phe 165 170 91 710 DNA Neisseria gonorrhoeae 91 cggcaaagca gccggatgcc gccgcgtatc ttgaggcatt gaaaatatta cgatgcaaaa 60 agaaaatttc agtataatac ggcaggattc tttaacggat tattaacaat ttttctccct 120 gaccataaag gaaccaaaat atgaaaaaag cacttgccgc actgattgcc ctcgcactcc 180 cggccgccgc actggcggaa ggcgcatccg gcttttacgt ccaagccgat gccgcacacg 240 ccaaagcctc aagctcttta ggttctgcca aaggcttcag cccgcgcatc tccgcaggct 300 accgcatcaa cgacctccgc ttcgccgtcg attacacgcg ctacaaaaac tataaagccc 360 catccaccga tttcaaactt tacagcatcg gcgcgtccgt catttacgac ttcgacaccc 420 aatcgcccgt caaaccgtat ttcggcgcgc gcttgagcct caaccgcgct tccgcccact 480 tgggcggcag cgacagcttc agcaaaacct ccgccggcct cggcgtattg gcgggcgtaa 540 gctatgccgt taccccgaat gtcgatttgg atgccggcta ccgctacaac tacgtcggca 600 aagtcaacac tgtcaaaaac gtccgttccg gcgaactgtc cgccggcgtg cgcgtcaaat 660 tctgatatac gcgttattcc gcaaaccgcc gagccttcgg cggttttttg 710 92 792 PRT Neisseria gonorrhoeae 92 Met Lys Leu Lys Gln Ile Ala Ser Ala Leu Met Met Leu Gly Ile Ser 1 5 10 15 Pro Leu Ala Phe Ala Asp Phe Thr Ile Gln Asp Ile Arg Val Glu Gly 20 25 30 Leu Gln Arg Thr Glu Pro Ser Thr Val Phe Asn Tyr Leu Pro Val Lys 35 40 45 Val Gly Asp Thr Tyr Asn Asp Thr His Gly Ser Ala Ile Ile Lys Ser 50 55 60 Leu Tyr Ala Thr Gly Phe Phe Asp Asp Val Arg Val Glu Thr Ala Asp 65 70 75 80 Gly Leu Leu Leu Leu Thr Val Ile Val Cys Pro Thr Ile Gly Ser Leu 85 90 95 Asn Ile Thr Gly Ala Lys Met Leu Gln Asn Asp Ala Ile Lys Lys Asn 100 105 110 Leu Glu Ser Phe Gly Leu Ala Gln Ser Gln Tyr Phe Asn Gln Ala Thr 115 120 125 Leu Asn Gln Ala Val Ala Gly Leu Lys Glu Glu Tyr Leu Gly Arg Gly 130 135 140 Lys Leu Asn Ile Gln Ile Thr Pro Lys Val Thr Lys Leu Ala Arg Asn 145 150 155 160 Arg Val Asp Ile Asp Ile Thr Ile Asp Glu Gly Lys Ser Ala Lys Ile 165 170 175 Thr Asp Ile Glu Phe Glu Gly Asn Gln Val Tyr Ser Asp Arg Lys Leu 180 185 190 Met Arg Gln Met Ser Leu Thr Glu Gly Gly Ile Trp Thr Trp Leu Thr 195 200 205 Arg Ser Asp Arg Phe Asp Arg Gln Lys Phe Ala Gln Asp Met Glu Lys 210 215 220 Val Thr Asp Phe Tyr Gln Asn Asn Gly Tyr Phe Asp Phe Arg Ile Leu 225 230 235 240 Asp Thr Asp Ile Gln Thr Asn Glu Asp Lys Thr Arg Gln Thr Ile Lys 245 250 255 Ile Thr Val His Glu Gly Gly Arg Phe Arg Trp Gly Lys Val Ser Ile 260 265 270 Glu Gly Asp Thr Asn Glu Val Pro Lys Ala Glu Leu Glu Lys Leu Leu 275 280 285 Thr Met Lys Pro Gly Lys Trp Tyr Glu Arg Gln Gln Met Thr Ala Val 290 295 300 Leu Gly Glu Ile Gln Asn Arg Met Gly Ser Ala Gly Tyr Ala Tyr Ser 305 310 315 320 Glu Ile Ser Val Gln Pro Leu Pro Asn Ala Gly Thr Lys Thr Val Asp 325 330 335 Phe Val Leu His Ile Glu Pro Gly Arg Lys Ile Tyr Val Asn Glu Ile 340 345 350 His Ile Thr Gly Asn Asn Lys Thr Arg Asp Glu Val Val Arg Arg Glu 355 360 365 Leu Arg Gln Met Glu Ser Ala Pro Tyr Asp Thr Ser Lys Leu Gln Arg 370 375 380 Ser Lys Glu Arg Val Glu Leu Leu Gly Tyr Phe Asp Asn Val Gln Phe 385 390 395 400 Asp Ala Val Pro Leu Ala Gly Thr Pro Asp Lys Val Asp Leu Asn Met 405 410 415 Ser Leu Thr Glu Arg Ser Thr Gly Ser Leu Asp Leu Ser Ala Gly Trp 420 425 430 Val Gln Asp Thr Gly Leu Val Met Ser Ala Gly Val Ser Gln Asp Asn 435 440 445 Leu Phe Gly Thr Gly Lys Ser Ala Ala Leu Arg Ala Ser Arg Ser Lys 450 455 460 Thr Thr Leu Asn Gly Ser Leu Ser Phe Thr Asp Pro Tyr Phe Thr Ala 465 470 475 480 Asp Gly Val Ser Leu Gly Tyr Asp Ile Tyr Gly Lys Ala Phe Asp Pro 485 490 495 Arg Lys Ala Ser Thr Ser Val Lys Gln Tyr Lys Thr Thr Thr Ala Gly 500 505 510 Gly Gly Val Arg Met Gly Ile Pro Val Thr Glu Tyr Asp Arg Val Asn 515 520 525 Phe Gly Leu Ala Ala Glu His Leu Thr Val Asn Thr Tyr Asn Lys Ala 530 535 540 Pro Lys Arg Tyr Ala Asp Phe Ile Lys Gln Tyr Gly Lys Thr Asp Gly 545 550 555 560 Ala Asp Gly Ser Phe Lys Gly Leu Leu Tyr Lys Gly Thr Val Gly Trp 565 570 575 Gly Arg Asn Lys Thr Asp Ser Ala Leu Trp Pro Thr Arg Gly Tyr Leu 580 585 590 Thr Gly Val Asn Ala Glu Ile Ala Leu Pro Gly Ser Lys Leu Gln Tyr 595 600 605 Tyr Ser Ala Thr His Asn Gln Thr Trp Phe Phe Pro Leu Ser Lys Thr 610 615 620 Phe Thr Leu Met Leu Gly Gly Glu Val Gly Ile Ala Gly Gly Tyr Gly 625 630 635 640 Arg Thr Lys Glu Ile Pro Phe Phe Glu Asn Phe Tyr Gly Gly Gly Leu 645 650 655 Gly Ser Val Arg Gly Tyr Glu Ser Gly Thr Leu Gly Pro Lys Val Tyr 660 665 670 Asp Glu Tyr Gly Glu Lys Ile Ser Tyr Gly Gly Asn Lys Lys Ala Asn 675 680 685 Val Ser Ala Glu Leu Leu Phe Pro Met Pro Gly Ala Lys Asp Ala Arg 690 695 700 Thr Val Arg Leu Ser Leu Phe Ala Asp Ala Gly Ser Val Trp Asp Gly 705 710 715 720 Arg Thr Tyr Thr Ala Ala Glu Asn Gly Asn Asn Lys Ser Val Tyr Ser 725 730 735 Glu Asn Ala His Lys Ser Thr Phe Thr Asn Glu Leu Arg Tyr Ser Ala 740 745 750 Gly Gly Ala Val Thr Trp Leu Ser Pro Leu Gly Pro Met Lys Phe Ile 755 760 765 Tyr Ala Tyr Pro Leu Lys Lys Lys Pro Glu Asp Glu Ile Gln Arg Phe 770 775 780 Gln Phe Gln Leu Gly Thr Thr Phe 785 790 93 2379 DNA Neisseria gonorrhoeae 93 atgaaactga aacagattgc ctccgcactg atgatgttgg gcatatcgcc tttggcattt 60 gccgacttca ccatccaaga catccgtgtc gaaggcttgc agcgtaccga gccgagcacc 120 gtattcaact acctgcccgt caaagtcggc gacacctaca acgacacaca cggcagtgcc 180 atcatcaaaa gcctgtacgc caccggtttc tttgacgacg tacgagtcga aactgcggac 240 gggctgcttc tgctgaccgt tatcgtatgc cctaccatcg gctcgctcaa catcaccggc 300 gccaaaatgc tgcagaacga cgccatcaag aaaaacctcg aatcgttcgg gctggcgcag 360 tcgcaatact ttaatcaggc gacactcaac caggcagtcg ccggcctgaa agaagaatat 420 ctcgggcgcg gcaaactcaa tatccaaatc acgcccaaag taaccaaact cgcccgcaac 480 cgcgtcgaca tcgacatcac gattgacgag ggcaaatccg ccaaaatcac cgacatcgaa 540 tttgaaggca accaagtcta ttccgaccgc aaactgatgc ggcagatgtc gctgaccgaa 600 ggcggcattt ggacatggct gacacgaagc gaccggttcg accgccagaa attcgcccaa 660 gacatggaaa aagtaaccga cttctaccag aacaacggct acttcgattt ccgtatcctc 720 gataccgaca tccaaaccaa cgaagacaaa accaggcaga ccatcaaaat caccgtccac 780 gaaggcggac gtttccgctg gggcaaagtg tcgattgaag gcgacaccaa cgaagtcccc 840 aaggccgaac tggaaaaact gctgaccatg aagcccggca aatggtacga acgccagcag 900 atgaccgccg ttttgggtga gattcagaac cgcatgggct cggcaggcta cgcatacagc 960 gaaatcagcg tacagccgct gccgaacgcc ggaaccaaaa ccgtcgattt cgtcctgcac 1020 atcgaaccgg gcagaaaaat ctacgtcaac gaaatccaca tcaccggcaa caacaaaacc 1080 cgcgacgaag tcgtgcgccg cgaattgcgc caaatggaat ccgcgcctta cgacacctcc 1140 aagctgcaac gctccaaaga gcgcgtcgag cttttgggct acttcgacaa cgtacagttt 1200 gatgccgtcc cgcttgccgg tacgcccgac aaagtcgatt tgaacatgag cctgaccgaa 1260 cgttccaccg gctcgctcga cttgagcgcg ggctgggttc aggataccgg cttggtcatg 1320 tccgccggcg tatcgcagga caacctgttc ggtacgggca agtcggccgc cctgcgcgcc 1380 tcgcgaagca aaaccacgct caacggctcg ctgtcgttta ccgacccgta cttcacggca 1440 gacggggtca gcctgggcta cgatatttac ggaaaagcct tcgacccgcg caaagcatcg 1500 accagcgtca aacaatataa aaccaccacc gccggcggcg gcgtaaggat gggtatcccc 1560 gttaccgaat acgaccgcgt caatttcggg ctggcggcgg aacacctgac cgtcaacacc 1620 tacaacaaag cacccaaacg ctatgccgac tttatcaaac aatacggcaa aaccgacggc 1680 gcagacggca gcttcaaagg cctgctgtac aaaggcactg tcggctgggg gcgcaacaag 1740 accgacagcg ccttatggcc gacgcgcggc tacctgaccg gcgtaaatgc cgaaatcgcc 1800 ctgcccggca gcaaactgca atactactcc gccacccaca accaaacctg gttcttcccc 1860 ttaagcaaaa ccttcacgct gatgctcggc ggcgaagtcg gcattgcggg cggctacggc 1920 agaaccaaag aaatcccctt ctttgaaaac ttctacggcg gcggcctggg ttcggtgcgc 1980 ggctacgaaa gcggcacgct cggcccgaaa gtgtatgacg aatacggcga aaaaatcagc 2040 tacggcggca acaaaaaagc caacgtctcc gccgagctgc tcttcccgat gcccggtgcg 2100 aaagacgcac gcaccgtccg cctgagcctg tttgccgacg caggcagcgt gtgggacggc 2160 agaacctata ccgccgccga aaacggtaac aacaaatcgg tttactcgga aaacgcgcat 2220 aaatccacct ttaccaacga attgcgctat tccgccggcg gcgcggttac ctggctctcg 2280 cctttgggcc cgatgaaatt catctacgcc tacccgctga agaaaaaacc ggaagacgaa 2340 atccaacgct tccaattcca gctcggcacg acgttctaa 2379 94 1128 DNA Neisseria gonorrhoeae 94 atgaatacac gaaatatgcg ctatattctt ttgacaggac tgttgccgac ggcatccgct 60 tttggagaga ccgcgctgca atgcgccgct ttgacggaca atgttacgcg tttggcgtgt 120 tacgacagga tttttgcggc acagcttccg tcttcggcag ggcaggaagg gcaggagtcg 180 aaagccgtac tcaatctgac ggaaaccgtc cgcagcagct tggataaggg cgaggcggtc 240 attgttgttg aaaaaggcgg ggatgcgctt cctgccgaca gtgcgggcga aaccgccgat 300 atctatacgc ctttgagcct gatgtacgac ttggacaaaa acgatttgcg cgggctgttg 360 ggcgtacgcg aacacaatcc gatgtacctt atgccgtttt ggtataacaa ttcgcccaac 420 tatgccccga gttcgccgac gcgcggtacg actgtacagg aaaaattcgg acagcagaaa 480 cgtgcggaaa ccaaattgca ggtttcgttc aaaagcaaaa ttgccgaaaa tttgtttaaa 540 acccgcgcgg atctgtggtt cggctacacc caaagatccg attggcagat ttacaaccaa 600 ggcaggaaat ccgcgccgtt ccgcaatacg gattacaaac ctgaaatttt cctgacccag 660 cctgtgaagg cggatttgcc gttcggcggc aggctgcgta tgctcggtgc gggttttgtc 720 caccagtcca acggacagag ccgtcccgaa tcgcgttcgt ggaacaggat ttatgccatg 780 gcaggcatgg aatggggcaa attgacggtg attccgcgcg tgtgggtgcg tgcgttcgat 840 cagagcggcg ataaaaacga caatcccgat attgccgact atatggggta tggcgacgtg 900 aagctgcagt accgcctgaa cgacaggcag aatgtgtatt ccgtattgcg ctacaacccc 960 aaaacgggct acggcgcgat tgaagccgcc tacacgtttc cgattaaggg caaactcaaa 1020 ggcgtggtac gcggattcca cggttacggc gagagcctga tcgactacaa ccacaagcag 1080 aacggtatcg gtatcgggtt gatgttcaac gactgggacg gcatctga 1128 95 375 PRT Neisseria gonorrhoeae 95 Met Asn Thr Arg Asn Met Arg Tyr Ile Leu Leu Thr Gly Leu Leu Pro 1 5 10 15 Thr Ala Ser Ala Phe Gly Glu Thr Ala Leu Gln Cys Ala Ala Leu Thr 20 25 30 Asp Asn Val Thr Arg Leu Ala Cys Tyr Asp Arg Ile Phe Ala Ala Gln 35 40 45 Leu Pro Ser Ser Ala Gly Gln Glu Gly Gln Glu Ser Lys Ala Val Leu 50 55 60 Asn Leu Thr Glu Thr Val Arg Ser Ser Leu Asp Lys Gly Glu Ala Val 65 70 75 80 Ile Val Val Glu Lys Gly Gly Asp Ala Leu Pro Ala Asp Ser Ala Gly 85 90 95 Glu Thr Ala Asp Ile Tyr Thr Pro Leu Ser Leu Met Tyr Asp Leu Asp 100 105 110 Lys Asn Asp Leu Arg Gly Leu Leu Gly Val Arg Glu His Asn Pro Met 115 120 125 Tyr Leu Met Pro Phe Trp Tyr Asn Asn Ser Pro Asn Tyr Ala Pro Ser 130 135 140 Ser Pro Thr Arg Gly Thr Thr Val Gln Glu Lys Phe Gly Gln Gln Lys 145 150 155 160 Arg Ala Glu Thr Lys Leu Gln Val Ser Phe Lys Ser Lys Ile Ala Glu 165 170 175 Asn Leu Phe Lys Thr Arg Ala Asp Leu Trp Phe Gly Tyr Thr Gln Arg 180 185 190 Ser Asp Trp Gln Ile Tyr Asn Gln Gly Arg Lys Ser Ala Pro Phe Arg 195 200 205 Asn Thr Asp Tyr Lys Pro Glu Ile Phe Leu Thr Gln Pro Val Lys Ala 210 215 220 Asp Leu Pro Phe Gly Gly Arg Leu Arg Met Leu Gly Ala Gly Phe Val 225 230 235 240 His Gln Ser Asn Gly Gln Ser Arg Pro Glu Ser Arg Ser Trp Asn Arg 245 250 255 Ile Tyr Ala Met Ala Gly Met Glu Trp Gly Lys Leu Thr Val Ile Pro 260 265 270 Arg Val Trp Val Arg Ala Phe Asp Gln Ser Gly Asp Lys Asn Asp Asn 275 280 285 Pro Asp Ile Ala Asp Tyr Met Gly Tyr Gly Asp Val Lys Leu Gln Tyr 290 295 300 Arg Leu Asn Asp Arg Gln Asn Val Tyr Ser Val Leu Arg Tyr Asn Pro 305 310 315 320 Lys Thr Gly Tyr Gly Ala Ile Glu Ala Ala Tyr Thr Phe Pro Ile Lys 325 330 335 Gly Lys Leu Lys Gly Val Val Arg Gly Phe His Gly Tyr Gly Glu Ser 340 345 350 Leu Ile Asp Tyr Asn His Lys Gln Asn Gly Ile Gly Ile Gly Leu Met 355 360 365 Phe Asn Asp Trp Asp Gly Ile 370 375 96 1000 DNA Neisseria gonorrhoeae 96 ttttggcttc cagcgtttcg ttgttttcgt acaagtcgta agtcagcttc agattgttgg 60 cttttttaaa gtcttcgacc gtactctcgt caacataatt cgaccagttg tagatgttca 120 gagtatcggt ggcagcggct tcggcattgg cagcaggtgc gctgcctgct tgaggctgca 180 cggcgttttt ttcgctgccg ccgcaggctg ccagagacag cgcggccaaa acggctaata 240 cggatttttt catacgggca gattcctgat gaaagaggtt ggaaaaaaag aaaaccccgc 300 gccccataaa caccccggcg caaggtttgg gtattgtaaa gtaaatttgt gcaaactcaa 360 agcgatattg gcctgatttt cctaaaaaat taccctgttt ccaaaaaagg gggggaaacg 420 gccgcccgat tttgccgttt ttttgcgccg tcagggtgtc cgacgggcgg atagagaaaa 480 aaggcttgca tataatgtaa acccccttta aaattgcgcg tttacagaat ttatttttct 540 ttcaggagat tccaatatgg caaacagcgc acaagcacgc aaacgtgccc gccagtccgt 600 caaacaacgc gcccacaacg ctagcctgcg taccgcattc cgcaccgcag tgaaaaaagt 660 attgaaagca gtcgaagcag gcgataaagc tgccgcacaa gcggtttacc aagagtccgt 720 caaagtcatc gaccgcatcg ccgacaaagg cgtgtttcat aaaaacaaag cggctcgcca 780 caaaagccgc ctgtctgcaa aagtaaaagc actggcttga tttttgcaaa accgccaagg 840 cggttgatac gcgataagcg gaaaaccctg aagcccgacg gtttcggggt tttctgtatt 900 tcgggggtaa agttcgaaat ggcggaaagg gtgcggtttt ttatccgaat ccgctataaa 960 atgccgtttg aaaaccaata tgccgacaat gggggcggag 1000 97 713 PRT Neisseria gonorrhoeae 97 Met Asn Ala Pro Phe Phe Arg Leu Ser Leu Leu Ser Leu Thr Leu Ala 1 5 10 15 Ala Gly Phe Ala His Ala Ala Glu Asn Asn Ala Asn Val Ala Leu Asp 20 25 30 Thr Val Thr Val Lys Gly Asp Arg Gln Gly Ser Lys Ile Arg Thr Asn 35 40 45 Ile Val Thr Leu Gln Gln Lys Asp Glu Ser Thr Ala Thr Asp Met Arg 50 55 60 Glu Leu Leu Lys Glu Glu Pro Ser Ile Asp Phe Gly Gly Gly Asn Gly 65 70 75 80 Thr Ser Gln Phe Leu Thr Leu Arg Gly Met Gly Gln Asn Ser Val Asp 85 90 95 Ile Lys Val Asp Asn Ala Tyr Ser Asp Ser Gln Ile Leu Tyr His Gln 100 105 110 Gly Arg Phe Ile Val Asp Pro Ala Leu Val Lys Val Val Ser Val Gln 115 120 125 Lys Gly Ala Gly Ser Ala Ser Ala Gly Ile Gly Ala Thr Asn Gly Ala 130 135 140 Ile Ile Ala Lys Thr Val Asp Ala Gln Asp Leu Leu Lys Gly Leu Asp 145 150 155 160 Lys Asn Trp Gly Val Arg Leu Asn Ser Gly Phe Ala Gly Asn Asn Gly 165 170 175 Ala Ser Tyr Gly Ala Ser Val Phe Gly Lys Glu Gly Asn Phe Asp Gly 180 185 190 Leu Phe Ser Tyr Asn Arg Asn Asp Glu Lys Asp Tyr Glu Ala Gly Lys 195 200 205 Gly Phe Arg Asn Asp Asn Gly Gly Lys Thr Val Pro Tyr Ser Ala Leu 210 215 220 Asp Lys Arg Ser Tyr Leu Ala Lys Ile Gly Thr Thr Phe Gly Asp Gly 225 230 235 240 Asp His Arg Ile Val Leu Ser His Met Lys Asp Gln His Arg Gly Ile 245 250 255 Arg Thr Val Arg Glu Glu Phe Ala Val Ser Glu Lys Asn Ser Arg Ile 260 265 270 Thr Ile Lys Arg Gln Ala Pro Ser Tyr Arg Glu Thr Thr Gln Ser Asn 275 280 285 Thr Asn Leu Ala Tyr Thr Gly Lys Asp Leu Gly Phe Val Glu Lys Leu 290 295 300 Asp Ala Asn Ala Tyr Val Leu Glu Lys Lys Arg Tyr Ser Ala Asp Asp 305 310 315 320 Lys Asp Asn Gly Tyr Ala Gly Asn Val Lys Gly Pro Asn His Thr Arg 325 330 335 Ile Ala Thr Arg Ser Met Asn Phe Asn Phe Asp Ser Arg Leu Ala Glu 340 345 350 Gln Thr Leu Leu Lys Tyr Gly Ile Asn Tyr Arg His Gln Glu Ile Lys 355 360 365 Pro Gln Ala Phe Leu Asn Ser Glu Phe Glu Ile Lys Asp Lys Glu Lys 370 375 380 Ala Thr Asn Glu Glu Lys Lys Lys Asn Arg Glu Asn Glu Lys Ile Ala 385 390 395 400 Lys Ala Tyr Arg Leu Thr Asn Pro Thr Lys Thr Asp Thr Gly Ala Tyr 405 410 415 Ile Glu Ala Ile His Glu Ile Asp Gly Phe Thr Leu Thr Gly Gly Leu 420 425 430 Arg Tyr Asp Arg Phe Lys Val Lys Thr His Asp Gly Lys Thr Val Ser 435 440 445 Ser Ser Ser Leu Asn Pro Ser Phe Gly Val Ile Trp Gln Pro Arg Glu 450 455 460 His Trp Ser Phe Ser Ala Ser His Asn Tyr Ala Gly Arg Ser Pro Arg 465 470 475 480 Leu Tyr Asp Ala Leu Gln Thr His Gly Lys Arg Gly Ile Ile Ser Ile 485 490 495 Ala Asp Gly Thr Lys Ala Glu Arg Ala Arg Asn Thr Glu Ile Gly Phe 500 505 510 Asn Tyr Asn Asp Gly Thr Phe Ala Ala Asn Gly Ser Tyr Phe Arg Gln 515 520 525 Thr Ile Lys Asp Ala Leu Ala Asn Pro Gln Asn Arg His Asp Ser Val 530 535 540 Ala Val Arg Glu Ala Val Asn Ala Gly Tyr Ile Lys Asn His Gly Tyr 545 550 555 560 Glu Leu Gly Ala Ser Tyr Arg Thr Gly Gly Leu Thr Ala Lys Val Gly 565 570 575 Val Ser His Ser Lys Pro Arg Phe Tyr Asp Thr His Lys Asp Lys Leu 580 585 590 Leu Ser Ala Asn Pro Glu Phe Gly Ala Gln Val Gly Arg Thr Trp Thr 595 600 605 Ala Ser Leu Ala Tyr Arg Phe Lys Asn Pro Asn Leu Glu Ile Gly Trp 610 615 620 Arg Gly Arg Tyr Val Gln Lys Ala Val Gly Ser Ile Leu Ala Ala Gly 625 630 635 640 Gln Lys Asp Arg Asp Gly Lys Leu Glu Asn Val Val Arg Gln Gly Phe 645 650 655 Gly Val Asn Asp Val Phe Ala Asn Trp Lys Pro Leu Gly Lys Asp Thr 660 665 670 Leu Asn Val Asn Leu Ser Val Asn Asn Val Phe Asp Lys Phe Tyr Tyr 675 680 685 Pro His Ser Gln Arg Trp Thr Asn Thr Leu Pro Gly Val Gly Arg Asp 690 695 700 Val Arg Leu Gly Val Asn Tyr Lys Phe 705 710 98 2600 DNA Neisseria gonorrhoeae 98 aaaccggtac ggcgttgccc cgccttagct caaagagaac gattccctaa ggtgctgaag 60 caccgagtga atcggttccg tactatttgt actgtctgcg gcttcgccgc cttgtcctga 120 tttttgttag tccacatata catttccgac aaaacctgtc aacaaaaaac aacgcttcgc 180 aaataaaaac gataatcagc tttacacaac ccccccccgc taatataaac aaaaataatt 240 attattattt tttcttatcc tgccaaacct taacggtttg gcttaacttc ccttcataca 300 ctcaaaagga cgaacaaatg aacgccccgt ttttccgcct cagcctgctc tcgctcacac 360 ttgccgccgg ctttgcccac gcggcagaaa ataatgccaa tgtcgcattg gataccgtta 420 ccgtaaaagg cgaccgccaa ggcagcaaaa tccgtaccaa catcgttacg cttcaacaaa 480 aagacgaaag caccgcaacc gatatgcgcg aactcttaaa agaagagccc tccatcgatt 540 tcggcggcgg caacggcacg tcccaattcc tgacgctgcg cggtatgggt cagaactctg 600 tcgacatcaa ggtggacaac gcctattccg acagccaaat cctttaccac caaggcagat 660 ttattgtcga tcccgctttg gttaaagtcg tttccgtaca gaaaggcgcg ggttccgcct 720 ctgccggtat cggcgcgacc aacggcgcga tcatcgccaa aaccgtcgat gcccaagacc 780 tgctcaaagg cttggataaa aactggggcg tgcgcctcaa cagcggcttt gccggcaaca 840 acggcgcaag ctacggcgca agcgtattcg gaaaagaggg caacttcgac ggtttgttct 900 cttacaaccg caacgatgaa aaagattacg aagccggcaa aggtttccgc aatgacaacg 960 gcggcaaaac cgtaccgtac agcgcgctgg acaaacgcag ctacctcgcc aaaatcggaa 1020 caaccttcgg cgacggcgac caccgcatcg tgttgagcca tatgaaagac caacaccggg 1080 gcatccgcac tgtgcgtgaa gagtttgccg tcagcgaaaa aaattcacgg ataactatta 1140 aacgccaagc cccatcctac cgcgaaacca ctcaatccaa caccaacttg gcgtacaccg 1200 gcaaagattt gggctttgtc gaaaaactgg atgccaacgc ctatgtgttg gaaaagaaac 1260 gctattccgc cgatgacaaa gataacggct acgcaggcaa tgtaaaaggc cccaaccata 1320 cccgaatcgc cactcggagt atgaacttca acttcgacag ccgccttgcc gaacaaaccc 1380 tgttgaaata cggcatcaac taccgccatc aggaaatcaa accgcaagcg tttttgaact 1440 cggaatttga aataaaagat aaagaaaaag caactaatga agagaaaaag aagaaccgtg 1500 aaaatgaaaa aattgccaaa gcctaccgcc tgaccaaccc gaccaaaacc gataccggcg 1560 cgtatatcga agccattcac gagattgacg gctttaccct gaccggcggg ctgcgttacg 1620 accgcttcaa ggtgaaaacc cacgacggca aaaccgtttc aagcagcagc ctcaacccga 1680 gtttcggcgt gatttggcag ccgcgcgaac actggagctt cagcgcgagc cacaactacg 1740 ccggccgcag cccgcgcctg tatgacgctc tgcaaaccca cggcaagcgc ggcatcatct 1800 cgattgccga cggcacgaaa gccgaacgcg cgcgcaatac cgaaatcggc ttcaactaca 1860 acgacggcac gtttgccgca aacggcagct acttccggca gaccatcaaa gacgcgcttg 1920 ccaatccgca aaaccgccac gactccgtcg ccgtccgcga agccgtcaac gccggctaca 1980 tcaaaaacca cggttacgaa ttgggcgcgt cctaccgcac cggcggcctg accgccaaag 2040 tcggcgtaag ccacagcaaa ccgcgctttt acgatacgca caaagacaag ctgttgagcg 2100 cgaaccctga atttggcgca caagtcggcc gcacttggac ggcctccctt gcctaccgct 2160 tcaaaaaccc gaatctggaa atcggctggc gcggtcgtta tgttcaaaaa gccgtgggtt 2220 cgatattggc ggcaggtcaa aaagaccgcg acggcaaatt ggaaaacgtt gtacgccaag 2280 gtttcggtgt gaacgatgtc ttcgccaact ggaaaccgct gggcaaagac acgctcaatg 2340 ttaatctttc ggttaacaac gtgttcgaca agttctacta tccgcacagc caacgctgga 2400 ccaataccct gccgggcgtg ggacgtgatg tacgcctggg cgtgaactac aagttctaaa 2460 acgcacatcc cgaaaaaatg ccgtctgaaa gcctttcaga cggcatctgt cctgataatt 2520 tgatatatag tggattaaca aaaaccggta cggcgttgcc ccgccttagc tcaaagggaa 2580 cgattcccta aggtgctgaa 2600 99 236 PRT Neisseria gonorrhoeae 99 Met Thr Lys Gln Leu Lys Leu Ser Ala Leu Phe Val Ala Leu Leu Ala 1 5 10 15 Ser Gly Thr Ala Val Ala Gly Glu Ala Ser Val Gln Gly Tyr Thr Val 20 25 30 Ser Gly Gln Ser Asn Glu Ile Val Arg Asn Asn Tyr Gly Glu Cys Trp 35 40 45 Lys Asn Ala Tyr Phe Asp Lys Ala Ser Gln Gly Arg Val Glu Cys Gly 50 55 60 Asp Ala Val Ala Val Pro Glu Pro Glu Pro Ala Pro Val Ala Val Val 65 70 75 80 Glu Gln Ala Pro Gln Tyr Val Asp Glu Thr Ile Ser Leu Ser Ala Lys 85 90 95 Thr Leu Phe Gly Phe Asp Lys Asp Ser Leu Arg Ala Glu Ala Gln Asp 100 105 110 Asn Leu Lys Val Leu Ala Gln Arg Leu Ser Arg Thr Asn Val Gln Ser 115 120 125 Val Arg Val Glu Gly His Thr Asp Phe Met Gly Ser Glu Lys Tyr Asn 130 135 140 Gln Ala Leu Ser Glu Arg Arg Ala Tyr Val Val Ala Asn Asn Leu Val 145 150 155 160 Ser Asn Gly Val Pro Ala Ser Arg Ile Ser Ala Val Gly Leu Gly Glu 165 170 175 Ser Gln Ala Gln Met Thr Gln Val Cys Gln Ala Glu Val Ala Lys Leu 180 185 190 Gly Ala Lys Ala Ser Lys Ala Lys Lys Arg Glu Ala Leu Ile Ala Cys 195 200 205 Ile Glu Pro Asp Arg Arg Val Asp Val Lys Ile Arg Ser Ile Val Thr 210 215 220 Arg Gln Val Val Pro Ala Arg Asn His His Gln His 225 230 235 100 1349 DNA Neisseria gonorrhoeae 100 gaattcctat ccgatttgcc gccatgtttc tacagcggcc tgtatgttgg caattcagca 60 gttgcttctg tatctgctgt acaaatctaa tgagggaata aaatgaccaa acagctgaaa 120 ttaagcgcat tattcgttgc attgctcgct tccggcactg ctgttgcggg cgaggcgtcc 180 gttcagggtt acaccgtaag cggccagtcg aacgaaatcg tacgcaacaa ctatggagaa 240 tgctggaaaa acgcctactt tgataaagca agccaaggtc gcgtagaatg cggcgatgcg 300 gttgccgtcc ccgagcccga acccgcgcct gtcgccgttg tggagcaggc tcctcaatat 360 gttgatgaaa ccatttccct gtctgccaaa accctgttcg gtttcgataa ggattcattg 420 cgcgccgaag ctcaagacaa cctgaaagta ttggcgcaac gcctgagtcg aaccaatgtc 480 caatctgtcc gcgtcgaagg ccataccgac tttatgggtt ctgaaaaata caatcaggct 540 ctgtccgaac gccgcgcata cgtagtggca aacaacctgg tcagcaacgg cgtacctgct 600 tctagaattt ctgctgtcgg cttgggcgaa tctcaagcgc aaatgactca agtttgtcaa 660 gccgaagttg ccaaactggg tgcgaaagcc tctaaagcca aaaaacgtga ggctctgatt 720 gcatgtatcg aacctgaccg ccgcgtagat gtgaaaatcc gcagcatcgt aacccgtcag 780 gttgtgccgg cacgcaatca tcaccaacac taaggctagg taatatcttg ccgatgcatg 840 aggttagcgg attttgtacc gggtactgtt gcaatattcg tgaaacgtcg gccggtatcg 900 atgatgtgaa acaaaccccg cttttgcggg gtttgttttt ttgggtggtt ttctgaaacg 960 gctatcgtca gaatcggggt gcaggttcgg attcggattc agattcatgt ttgtgtccca 1020 ttgccgcgct ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt 1080 acaatagtac ggcaaggcga ggcaacgccg taccggttta aatttaatcc actatatcgg 1140 ttgaaactct gattttaagg cggtaggatg tgggtttgcc catagcaagg gaatcctttc 1200 tgtatcaagc cccgaaaggg ataattcata caaattcacg cctttccccc tcattgggaa 1260 atggatggaa tcgtgcccga tgtgtgcggc actgtatgcc ggatatggtt ttatcatcat 1320 cccttttcgg ttgaaacccc gcggaattc 1349 101 32 DNA Artificial Sequence Primer 101 gggaatccat atgaaaaaac tcttgaaatc gg 32 102 29 DNA Artificial Sequence Primer 102 ggaagatctt tagaagcgga attgtgcat 29 103 36 DNA Artificial Sequence Primer 103 ctgcagaatc gcgaatgaaa aaactcttga aatcgg 36 104 35 DNA Artificial Sequence Primer 104 ctgcagaatc gcgaatgagt agcaagctag tgaac 35 105 29 DNA Artificial Sequence Primer 105 aggagatctt tagaattgga atcctccgg 29 106 1194 DNA Chlamydia trachomatis 106 atgaaaaaac tcttgaaatc ggtattagta tttgccgctt tgagttctgc ttcctccttg 60 caagctctgc ctgtggggaa tcctgctgaa ccaagcctta tgatcgacgg aattctgtgg 120 gaaggtttcg gcggagatcc ttgcgatcct tgcaccactt ggtgtgacgc tatcagcatg 180 cgcgttggtt actacggaga ctttgttttc gaccgtgttt tgaaaactga tgtgaataaa 240 gaatttcaga tgggagcggc gcctactacc agcgatgtag aaggcttaca aaacgatcca 300 acaacaaatg ttgctcgtcc aaatcccgct tatggcaaac acatgcaaga tgctgaaatg 360 tttacgaacg ctgcttacat ggcattaaat atctgggatc gttttgatgt attttgtaca 420 ttgggagcaa ctaccggtta tttaagagga aactccgctt ccttcaactt agttggatta 480 ttcggaacaa aaacacaata ttctaagttt aatacagcga atcttgttcc taacactgct 540 ttggatcgag ctgtggttga gctttataca gacaccacct ttgcttggag cgtaggtgct 600 cgtgcagctc tctgggaatg tgggtgtgca acgttaggag cttctttcca atatgctcaa 660 tctaaaccta aagtagaaga gttaaatgtt ctttgtaatg catccgaatt tactattaat 720 aagccgaaag gatatgttgg ggtggaattt ccacttgata ttaccgcagg aacagaagct 780 gcgacaggga ctaaggatgc ctctattgac taccatgagt ggcaagcaag tttagccctt 840 tcttacagat taaatatgtt cactccttac attggagtta aatggtctag agtaagtttt 900 gatgccgaca cgatccgtat cgctcagcct aaattggctg aagcaatctt ggatgtcact 960 actctaaacc cgaccatcgc tggtaaagga gctgtggtct cttccggaag cgataacgaa 1020 ctggctgata caatgcaaat cgtttccttg cagttgaaca agctgaaatc tagaaaatct 1080 tgcggtattg cagtaggaac gactattgta gatgcagata aatacgcagt tacagttgag 1140 actcgcttga tcgatgagag agcagctcac gtaaatgcac aattccgctt ctaa 1194 107 1182 DNA Chlamydia trachomatis 107 atgaaaaaac tcttgaaatc ggtattagta tttgccgctt tgagttctgc ttcctccttg 60 caagctctgc ctgtggggaa tcctgctgaa ccaagcctta tgatcgacgg aattctgtgg 120 gaaggtttcg gcggagatcc ttgcgatcct tgcgccactt ggtgtgacgc tatcagcatg 180 cgtgttggtt actacggaga ctttgttttc gaccgtgttt tgaaaactga tgtgaataaa 240 gaatttcaga tgggtgccaa gcctacaact gatacaggca atagtgcagc tccatccact 300 cttacagcaa gagagaatcc tgcttacggc cgacatatgc aggatgctga gatgtttaca 360 aatgccgctt gcatggcatt gaatatttgg gatcgttttg atgtattctg tacattagga 420 gccaccagtg gatatcttaa aggaaactct gcttctttca atttagttgg attgtttgga 480 gataatgaaa atcaaaaaac ggtcaaagcg gagtctgtac caaatatgag ctttgatcaa 540 tctgttgttg agttgtatac agatactact tttgcgtgga gcgtcggcgc tcgcgcagct 600 ttgtgggaat gtggatgtgc aactttagga gcttcattcc aatatgctca atctaaacct 660 aaagtagaag aattaaacgt tctctgcaat gcagcagagt ttactattaa taaacctaaa 720 gggtatgtag gtaaggagtt tcctcttgat cttacagcag gaacagatgc tgcgacagga 780 actaaggatg cctctattga ttaccatgaa tggcaagcaa gtttagctct ctcttacaga 840 ctgaatatgt tcactcccta cattggagtt aaatggtctc gagcaagctt tgatgccgat 900 acgattcgta tagcccagcc aaaatcagct acagctattt ttgatactac cacgcttaac 960 ccaactattg ctggagctgg cgatgtgaaa actggcgcag agggtcagct cggagacaca 1020 atgcaaatcg tttccttgca attgaacaag atgaaatcta gaaaatcttg cggtattgca 1080 gtaggaacaa ctattgtgga tgcagacaaa tacgcagtta cagttgagac tcgcttgatc 1140 gatgagagag cagctcacgt aaatgcacaa ttccgcttct aa 1182 108 1023 DNA Chlamydia trachomatis 108 atgagtagca agctagtgaa ctatctccgt ttgactttcc tatctttttt agggatcgca 60 tctacttcat tagacgctat gcctgcgggg aatccggcgt ttccagtcat cccggggatt 120 aatattgaac agaaaaatgc ctgttctttc gatttatgta attcttatga tgtactatcc 180 gcactgtccg gtaacctgaa gctctgcttc tgcggagatt atatcttttc agaagaagct 240 caggtaaaag atgtccctgt cgttacctct gtgacaacag ctggggttgg tccttctcct 300 gatattactt cgacaaccaa aacgcgaaat ttcgatctcg tgaactgtaa tctcaataca 360 aactgtgtag ctgtagcttt ttcccttcct gatcgttcgc tgagcgcgat tcctctgttt 420 gatgtgagtt tcgaagtgaa agtaggagga ctgaaacaat actaccgcct tcccatgaat 480 gcctatcgag acttcacctc ggaacctctc aattctgaat cagaagttac ggacgggatg 540 attgaagtac agtccaatta cggatttgtt tgggatgtta gcttgaaaaa agtcatatgg 600 aaagatggcg tttcctttgt aggcgtcggt gcagactatc gccatgcttc ttgccctatt 660 gactacatca ttgcaaacag tcaagctaat ccagaagtat tcatcgctga ctcggatggg 720 aaactgaact tcaaggagtg gagtgtctgc gtaggtctta ctacctatgt gaatgactac 780 gttcttcctt acttagcgtt ttctataggg agtgtttctc gccaagctcc ggacgacagc 840 ttcaaaaaat tagaagatcg cttcactaac ctcaaattta aagttcgtaa aattaccagc 900 tctcatcgtg gaaacatctg catcggagcg acaaactatg tcgccgataa cttcttctac 960 aacgtagaag gaagatgggg aagccagcgc gctgtgaacg tctccggagg attccaattc 1020 taa 1023 

1. A Gram-negative bacterial bleb not derived from Chlamydia presenting on its surface the PorB outer membrane protein from Chlamydia trachomatis, wherein the combination of the Chlamydia antigen with the native Gram-negative bacterial bleb antigens interact in the prevention or treatment of salpingitis when present in a vaccine formulation.
 2. The Gram-negative bleb of claim 1 further presenting on its surface the PmpG outer membrane proteins from Chlamydia trachomatis.
 3. The Gram-negative bleb of claim 1 further presenting on its surface MOMP from one or more serovars from Chlamydia trachomatis.
 4. A Gram-negative bleb not derived from Chlamydia, presenting on its surface both the PmpG and MOMP (from one or more serovars) outer membrane proteins from Chlamydia trachomatis, wherein the combination of the Chlamydia antigens with the native Gram-negative bacterial bleb antigens interact in the prevention or treatment of salpingitis when present in a vaccine formulation.
 5. The bleb of claims 1-4 which are gonococcal blebs.
 6. The bleb of claim 5 which has been derived from a gonococcal strain which has been modified to upregulate one or more protective gonococcal outer membrane antigens.
 7. The bleb of claims 5 and 6 derived from a gonococcal strain which has been modified to downregulate one or more immunodominant variable or non-protective gonococcal outer membrane antigens.
 8. The bleb of claims 5-7 derived from a strain which has a detoxified lipid A portion of bacterial LPS, due to the strain having been engineered to reduce or switch off expression of one or more genes selected from the group consisting of: htrB, msbB and lpxK.
 9. The bleb of claims 5-8 wherein the bleb preparation is derived from a strain which has a detoxified lipid A portion of bacterial LPS, due to the strain having been engineered to express at a higher level one or more genes selected from the group consisting of: pmrA, pmrB, pmrE and pmrF.
 10. A vaccine composition comprising the bleb of claims 1-9 and a pharmaceutically suitable excipient or carrier.
 11. The vaccine of claim 10, additionally comprising a mucosal adjuvant.
 12. A method of preventing Chlamydia trachomatis infection in a host comprising the steps of administering an effective amount of the vaccine of claim 10 or 11 to a host in need thereof.
 13. The method of claim 12 where in the vaccine is mucosally administered via either a intranasal, oral, or intravaginal route.
 14. A Gram-negative bleb produced from Neisseria meningitidis, Moraxella catharralis or Haemophilus influenzae presenting on its surface a protective antigen from Chlamydia pneumoniae.
 15. The Gram-negative bleb of claim 14 presenting on its surface a PorB outer membrane protein from Chlamydia pneumoniae.
 16. The Gram-negative bleb of claim 15 further_presenting on its surface MOMP outer membrane protein from Chlamydia pneumoniae.
 17. The Gram-negative bleb of claim 15 further_presenting on its surface one or more Pmp outer membrane proteins from Chlamydia pneumoniae.
 18. The Gram-negative bleb of claim 15 further_presenting on its surface Npt1 protein from Chlamydia pneumoniae.
 19. The Gram-negative bleb of claim 14 presenting on its surface one or more Pmp proteins from Chlamydia pneumoniae.
 20. The Gram-negative bleb of claim 19 further_presenting on its surface Npt1 protein from Chlamydia pneumoniae.
 21. The Gram-negative bleb of claim 19 further presenting on its surface MOMP protein from Chlamydia pneumoniae.
 22. The Gram-negative bleb of claim 14 presenting on its surface MOMP protein from Chlamydia pneumoniae.
 23. The Gram-negative bleb of claim 22 further presenting on its surface Npt1 protein from Chlamydia pneumoniae.
 24. The bleb of claims 14-23 which are meningococcal blebs.
 25. The bleb of claim 24 derived from a meningococcal strain that has been modified to upregulate one or more protective meningococcal outer membrane antigens.
 26. The bleb of claim 24 or 25 derived from a meningococcal strain that has been modified to downregulate one or more immunodominant variable or non-protective meningococcal outer membrane antigens.
 27. The bleb of claims 24-26 derived from a strain which has a detoxified lipid A portion of bacterial LPS, due to the strain having been engineered to reduce or switch oft expression of one or more genes selected from the group consisting of: htrB, msbB and lpxK.
 28. The bleb of claims 24-27 wherein the bleb preparation is derived from a strain which has a detoxified lipid A portion of bacterial LPS, due to the strain having been engineered to express at a higher level one or more genes selected from the group consisting of: pmrA, pmrB, pmrE and pmrF.
 29. A vaccine composition comprising the bleb of claims 14-28 and a pharmaceutically suitable excipient or carrier.
 30. The vaccine of claim 29, additionally comprising a mucosal adjuvant.
 31. A method of preventing Chlamydia pneumoniae infection in a host comprising the steps of administering an effective amount of the vaccine of claim 29 or 30 to a host in need thereof.
 32. The method of claim 31 where in the vaccine is mucosally administered via either an intranasal, or oral route. 